Abstract:
UNLABELLED:Seed storage albumins are abundant, water-soluble proteins that are degraded to provide critical nutrients for the germinating seedling. It has been established that the sunflower albumins encoded by SEED STORAGE ALBUMIN 2 (SESA2), SESA20 and SESA3 are the major components of the albumin-rich fraction of the common sunflower Helianthus annuus. To determine the structure of sunflowers most important albumins we performed a detailed chromatographic and mass spectrometric characterization to assess what post-translational processing they receive prior to deposition in the protein storage vacuole. We found that SESA2 and SESA20 each encode two albumins. The first of the two SESA2 albumins (SESA2-1) exists as a monomer of 116 or 117 residues, differing by a threonine at the C-terminus. The second of the two SESA2 albumins (SESA2-2) is a monomer of 128 residues. SESA20 encodes the albumin SESA20-2, which is a 127-residue monomer, whereas SESA20-1 was not abundant enough to be structurally described. SESA3, which has been partly characterized previously, was found in several forms with methylation of its asparagine residues. In contrast to other dicot albumins, which are generally matured into a heterodimer, all the dominant mature sunflower albumins SESA2, SESA20-2, SESA3 and its post-translationally modified analogue SESA3-a are monomeric. BIOLOGICAL SIGNIFICANCE:Sunflower plants have been bred to thrive in various climate zones making them favored crops to meet the growing worldwide demand by humans for protein. The abundance of seed storage proteins makes them an important source of protein for animal and human nutrition. This study explores the structures of the dominant sunflower napin-type seed storage albumins to understand what structures evolution has favored in the most abundant proteins in sunflower seed.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Franke B,Colgrave ML,Mylne JS,Rosengren KJdoi
10.1016/j.jprot.2016.05.004subject
Has Abstractpub_date
2016-09-16 00:00:00pages
177-186eissn
1874-3919issn
1876-7737pii
S1874-3919(16)30190-7journal_volume
147pub_type
杂志文章abstract::Leishmaniasis is second most neglected disease after malaria and seems to be a worldwide concern because of increased drug resistance and non-availability of approved vaccine. The underlying molecular mechanism of drug resistance (Amp B) in Leishmania parasites still remains elusive. Herein, the present study investig...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2019.103464
更新日期:2019-09-15 00:00:00
abstract::To seek a potential target for periodontal tissue regeneration, this study aimed to explore the role of Integrin alpha 5 (ITGA5) in human periodontal ligament stem cells (PDLSCs). Transwell assay, Cell Counting Kit 8 (CCK8) assay, cell cycle assay, alkaline phosphatase (ALP) activity, alizarin red staining, and wester...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.03.008
更新日期:2018-05-15 00:00:00
abstract:UNLABELLED:The sea cucumber Apostichopus japonicus is becoming an excellent model marine invertebrate for studies of environmentally-induced aestivation. Reversible protein phosphorylation as a regulatory mechanism in aestivation is known for some terrestrial aestivators but has never before been documented in sea cucu...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.09.016
更新日期:2016-03-01 00:00:00
abstract:UNLABELLED:In the marine environment, bacteria from estuarine and coastal sediments are among the first targets of nanoparticle pollution; it is therefore relevant to improve the knowledge of interactions between bacteria and nanoparticles. In this work, the response of the marine bacterium Pseudomonas fluorescens BA3S...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2016.07.021
更新日期:2016-10-04 00:00:00
abstract:UNLABELLED:Pluripotent stem cell-derived committed neural precursors are an important source of cells to treat neurodegenerative diseases including spinal cord injury. There remains an urgency to identify markers for monitoring of neural progenitor specificity, estimation of neural fate and follow-up correlation with t...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.11.008
更新日期:2016-01-30 00:00:00
abstract:UNLABELLED:Brevibacterium linens DSM 20158 is an industrially important actinobacterium which is well-known for the production of amino acids and enzymes. However, as this strain has an unsequenced genome, there is no detailed information regarding its proteome although another strain of this microbe, BL2, has a shotgu...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.02.029
更新日期:2013-05-27 00:00:00
abstract::Understanding the bioactive roles of colostrum proteins has gained much attention, and in particular, their potential use in human and veterinary medicine has been extensively studied. However, studies of bioactivity have mainly been conducted in vitro, but it has not yet been well characterized at the individual prot...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2010.08.011
更新日期:2011-01-01 00:00:00
abstract:UNLABELLED:Clostridium difficile spores are considered the morphotype of infection, transmission and persistence of C. difficile infections. There is a lack of information on the composition of the outermost exosporium layer of C. difficile spores. Using recently developed exosporium removal methods combined with MS/MS...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.03.035
更新日期:2015-06-18 00:00:00
abstract:UNLABELLED:It is currently unknown how many proteins can be detected in urine. Improving the analytical approach would increase their number and potentially strengthen their predictive potential in diseases. We developed a combination of analytical procedures for maximizing sensitivity and reproducibility of normal hum...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2014.07.021
更新日期:2015-01-01 00:00:00
abstract::Current protocols for quality assurance of platelet concentrates used in transfusion therapy include evaluation of platelet count and pH, in vitro measurements of platelet lysis, membrane activation and microparticle release and assays of platelet ability to respond to aggregation stimuli and to hypotonic shock. Unfor...
journal_title:Journal of proteomics
pub_type: 社论,评审
doi:10.1016/j.jprot.2012.07.044
更新日期:2012-12-05 00:00:00
abstract:UNLABELLED:Milk and dairy products are central elements in the human diet. It is estimated that 108kg of milk per year are consumed per person worldwide. Therefore, dairy production represents a relevant fraction of the economies of many countries, being cattle, sheep, goat, water buffalo, and other ruminants the main ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.09.012
更新日期:2013-12-06 00:00:00
abstract::A targeted quantitative proteomic investigation employing the multiple reaction monitoring (MRM, SRM) technique was conducted on strawberry fruit at different development stages. We investigated 22 proteins and isoforms from 32 peptides with 111 peptide transitions, which may be involved in the volatile aroma biosynth...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.06.004
更新日期:2015-08-03 00:00:00
abstract:UNLABELLED:Downy mildew is a serious fungal disease in non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) that is caused by Hyaloperonospora parasitica, which infects members of the Brassicaceae family. For breeding improvement, researchers must understand the defence mechanisms employed by non-hea...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.11.028
更新日期:2014-02-26 00:00:00
abstract::Serum amyloid A (SAA) is a well-described acute phase protein induced during the acute phase response (APR) to infection. Four isoform specific genes are found in most mammals. Depending on species, SAA3 and SAA4 are generally preferentially expressed extrahepatically whereas SAA1 and SAA2 are hepatic isoforms dominat...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.103904
更新日期:2020-09-15 00:00:00
abstract::Plasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants with a comprehensi...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.07.029
更新日期:2015-10-14 00:00:00
abstract::The reprogrammed lipopolysaccharide (LPS) pathway has been reported to render patients more susceptible to the development of post-traumatic multiple organ dysfunction syndrome (MODS). To facilitate thorough understanding of this mechanism, a phosphoproteomic study was utilized to screen the potential signaling molecu...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.07.003
更新日期:2015-10-14 00:00:00
abstract:UNLABELLED:The long cultivation of field pea led to an enormous diversity which, however, seems to hold just little resistance against the ascochyta blight disease complex. The potential of below ground microbial symbiosis to prime the immune system of Pisum for an upcoming pathogen attack has hitherto received little ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2016.03.018
更新日期:2016-06-30 00:00:00
abstract::Organic farming aims to be environmentally sound, but the question as to whether organic cropping brings more nutritional benefits to farmers and consumers than the conventional cropping needs still to be answered. To gain insights into the molecular effects of organic farming we used proteome analysis to analyze cabb...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.06.021
更新日期:2011-11-18 00:00:00
abstract::Lysine succinylation is a newly identified protein post-translational modification (PTM) of lysine residues. Increasing evidences demonstrate that this modification is prevalent in mitochondria and regulates many vital cellular processes, especially metabolism. Here, we determined the succinylome of the silkworm (Bomb...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2017.12.019
更新日期:2018-03-01 00:00:00
abstract:UNLABELLED:Fatal events derived from coronary atherosclerosis are the major cause of mortality in the developed countries. Proteomic analysis of the atherosclerotic coronary artery has been mainly carried out with whole tissue extracts, making it difficult to distinguish the alterations present in every region of the p...
journal_title:Journal of proteomics
pub_type: 临床试验,杂志文章
doi:10.1016/j.jprot.2013.01.032
更新日期:2013-04-26 00:00:00
abstract::Atrazine (ATZ), the second most commonly used herbicide in the United States, is an endocrine disrupting chemical linked to cancer and a common drinking water contaminant. This study further investigates ATZ-related developmental toxicity by testing the following hypotheses in zebrafish: the effects of embryonic ATZ e...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.07.006
更新日期:2018-08-30 00:00:00
abstract::Heat shock protein 70s (Hsp70s) are a highly conserved class of molecular chaperones that fold a large proportion of the proteome. Nematostella vectensis (Nv) is an estuarine sea anemone that has emerged as a model species to characterize molecular responses to physiological stressors due to its exposure to diverse, e...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2019.103416
更新日期:2019-08-30 00:00:00
abstract::Soil fungistasis limits the effect of fungal agents designed to control plant-parasitic nematodes. Benzaldehyde is a fungistatic factor produced by soil microorganisms that can suppress conidial germination, but the molecular mechanism of this suppression is unknown. In this study, three conidial proteomes of Arthrobo...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.09.016
更新日期:2019-02-10 00:00:00
abstract::Fatty acid desaturate 2 (Fads2) is associated with many chronic diseases. Nevertheless, comprehensive researches on its role have not been performed. We here conducted an integrated analysis of long-chain polyunsaturated fatty acid (LC-PUFA) metabolism of fads2-deletion zebrafish (fads2-/-) by transcriptomics, proteom...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.103967
更新日期:2020-10-30 00:00:00
abstract::Snake venoms are composed of approximately 90% of proteins with several pharmacological activities having high potential in research as biological tools. One of the most abundant compounds is phospholipases A2 (PLA2), which are the most studied venom protein due to their wide pharmacological activity. Using a combinat...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.10.020
更新日期:2016-01-10 00:00:00
abstract::Hollow fiber flow field-flow fractionation (HF FlFFF) has been demonstrated as a tool for pre-fractionating proteomes by differences in molecular mass (Mr), where the resulting protein fractions are subsequently digested and analyzed by shotgun proteomics using two-dimensional liquid chromatography-electrospray ioniza...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2008.02.001
更新日期:2008-04-30 00:00:00
abstract::In analysing the red blood cell cytoplasmic proteome, in search for low abundance proteins, 15 amino acid (AA; Arg, Asn, Asp, Gln, Gly, His, Ile, Lys, Phe, Pro, Ser, Thr, Trp, Tyr, and Val) probes, used individually, captured a total of 787 unique gene products. Of those, 76 were found to be the common catch of all AA...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2009.04.001
更新日期:2009-07-21 00:00:00
abstract::The role of Leishmania braziliensis in the development of different clinical forms of American Tegumentary Leishmaniasis (ATL) is unclear, but it has been suggested that molecules secreted/released by parasites could modulate the clinical outcome. Here, we analyzed the infection rate and cytokine profile of macrophage...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.104077
更新日期:2021-02-10 00:00:00
abstract:UNLABELLED:Erwinia amylovora is a Gram-negative plant pathogen that causes fire blight. This disease affects most members of the Rosaceae family including apple and pear. Here, an infection model is introduced to study proteomic changes in a highly virulent E. amylovora strain upon interaction with its host as compared...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2016.02.018
更新日期:2016-04-29 00:00:00
abstract::The regulation of mitochondrial function is essential for cardiomyocyte adaptation to cellular stress. While it has long been understood that phosphorylation regulates flux through metabolic pathways, novel phosphorylation sites are continually being discovered in all functionally distinct areas of the mitochondrial p...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.02.014
更新日期:2012-08-03 00:00:00