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Qualitative and quantitative peptidomic and proteomic approaches to phenotyping chicken semen.

Abstract：

UNLABELLED:Understanding of the avian male gamete biology is essential to improve the conservation of genetic resources and performance in farming. In this study, the chicken semen peptidome/proteome and the molecular phenotype related to sperm quality were investigated. Spermatozoa (SPZ) and corresponding seminal plasma (SP) from 11 males with different fertilizing capacity were analyzed using three quantitative strategies (fluid and intact cells MALDI-MS, SDS-PAGE combined to LC-MS/MS with spectral counting and XIC methods). Individual MALDI profiling in combination with top-down MS allowed to characterize specific profiles per male and to identify 16 biomolecules (e.g.VMO1, AvBD10 and AvBD9 including polymorphism). Qualitative analysis identified 1165 proteins mainly involved in oxidoreduction mechanisms, energy processes, proteolysis and protein localization. Comparative analyses between the most and the least fertile males were performed. The enzymes involved in energy metabolism, respiratory chain or oxido-reduction activity were over-represented in SPZ of the most fertile males. The SP of the most and the least fertile males differed also on many proteins (e.g. ACE, AvBD10 and AvBD9, NEL precursor, acrosin). Thus proteomic is a "phenomic molecular tool" that may help to discriminate avian males on their reproductive capacity. The data have been deposited with ProteomeXchange (identifiers PXD000287 and PXD001254). BIOLOGICAL SIGNIFICANCE:This peptidomic and proteomic study i) characterized for the first time the semen protein composition of the main domestic avian species (Gallus gallus) by analysis of ejaculated spermatozoa and corresponding seminal plasma; ii) established a characteristic molecular phenotype distinguishing semen and males at an individual level; and iii) proposedthe first evidence of biomarkers related to fertility.

journal_name

J Proteomics

journal_title

Journal of proteomics

authors

Labas V,Grasseau I,Cahier K,Gargaros A,Harichaux G,Teixeira-Gomes AP,Alves S,Bourin M,Gérard N,Blesbois E

doi

10.1016/j.jprot.2014.07.024

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

313-35

eissn

1874-3919

issn

1876-7737

pii

S1874-3919(14)00385-6

journal_volume

112

pub_type

杂志文章

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