当前位置： SCI文献检索 > ACTA PHARMACOLOGICA SINICA期刊下所有文献 > High prevalence of the B2+C2 subgenotype mixture in patients with chronic hepatitis B in Eastern China.

High prevalence of the B2+C2 subgenotype mixture in patients with chronic hepatitis B in Eastern China.

Abstract：

AIM:To investigate the prevalence of hepatitis B virus (HBV) genotype mixtures among patients with chronic hepatitis B (CHB) in Eastern China. METHODS:A total of 4908 chronic HBV patients from Eastern China were enrolled. HBV genotypes and subgenotypes were determined using a multiplex PCR technique. Serum viral loads and hepatitis B e antigen (HBeAg) levels detected using real-time fluorescent quantitative PCR and ELISA assay, respectively. The presence of precore/basic core promoter (PC/BCP) mutations was examined with PCR and direct sequencing of the amplified products. RESULTS:HBV genotypes B, C, D, B+C, and B+D were found in 19.21%, 64.75%, 1.49%, 13.63%, and 0.92% of the patients, respectively. In 669 patients with the genotype mixture B+C, the subgenotypes B2+C2 and B2+C1 accounted for 68.13% and 31.87%, respectively, no other subgenotypes were identified. HBV B+C was more frequent in the patients with moderate CHB than in patients with mild CHB. In patients with moderate CHB, the subgenotype mixture B2+C2 was lower than B2+C1 (51.97% vs 63.38%), while the opposite situation was found in patients with severe CHB (22.15% vs 15.49%). The highest average viral load was found in patients with the genotype B+C mixture. The prevalence of HBV B2+C2 increased in patients from 50 to 59 years of age and was significantly different from the proportion of patients in the same age group with genotype B (23.2% vs 15.2%). A double mutation (G1896A) in the PC was significantly more common in subgenotype B2+C2 than in subgenotype B2+C1. CONCLUSION:The HBV B2+C2 subgenotype was prevalent in CH patients with a high HBV replication status and correlated with a more severe course of the disease.

journal_name

Acta Pharmacol Sin

journal_title

Acta pharmacologica Sinica

authors

Zhong J,Gao YQ,Sun XH,Zhu XJ,Li M

doi

10.1038/aps.2012.78

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

1271-6

issue

10

eissn

1671-4083

issn

1745-7254

pii

aps201278

journal_volume

33

pub_type

杂志文章

相关文献

ACTA PHARMACOLOGICA SINICA文献大全
  • Betulinic acid regulates generation of neuroinflammatory mediators responsible for tissue destruction in multiple sclerosis in vitro.

    abstract：AIM:To investigate the influences of betulinic acid (BA), a triterpenoid isolated from birch bark, on neuroinflammatory mediators involved in the pathogenesis of multiple sclerosis and experimental autoimmune encephalomyelitis in vitro. METHODS:Encephalitogenic T cells were prepared from draining lymph nodes and spina...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2012.181

    authors： Blaževski J,Petković F,Momčilović M,Paschke R,Kaluđerović GN,Mostarica Stojković M,Miljković D

    更新日期：2013-03-01 00:00:00

  • Effects of gamichunggantang on hyperlipidemia.

    abstract：AIM:To evaluate the therapeutic effects of gamichunggantang (GCT) on hyperlipidemia through high cholesterol diet model. GCT is an Oriental herbal medication, which has been used for the treatment of fatty liver, hyperlipidemia or alcoholic liver disease in Daejeon University Oriental Hospital, Korea since 1999. METHO...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Son CG,Choi WJ,Shin JW,Han SH,Cho JH,Song KC,Cho CK

    更新日期：2003-02-01 00:00:00

  • A novel synthetic compound MCAP suppresses LPS-induced murine microglial activation in vitro via inhibiting NF-kB and p38 MAPK pathways.

    abstract：AIM:To investigate the anti-neuroinflammatory activity of a novel synthetic compound, 7-methylchroman-2-carboxylic acid N-(2-trifluoromethyl) phenylamide (MCAP) against LPS-induced microglial activation in vitro. METHODS:Primary mouse microglia and BV2 microglia cells were exposed to LPS (50 or 100 ng/mL). The express...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2015.138

    authors： Kim BW,More SV,Yun YS,Ko HM,Kwak JH,Lee H,Suk K,Kim IS,Choi DK

    更新日期：2016-03-01 00:00:00

  • Anti-PD-L1 mediating tumor-targeted codelivery of liposomal irinotecan/JQ1 for chemo-immunotherapy.

    abstract：:Immune checkpoint blockade therapy has become a first-line treatment in various cancers. But there are only a small percent of colorectal patients responding to PD-1/PD-L1 blockage immunotherapy. How to increase their treatment efficacy is an urgent and clinically unmet need. It is acknowledged that immunogenic cell d...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-020-00570-8

    authors： He ZD,Zhang M,Wang YH,He Y,Wang HR,Chen BF,Tu B,Zhu SQ,Huang YZ

    更新日期：2020-12-11 00:00:00

  • L655,240, acting as a competitive BACE1 inhibitor, efficiently decreases β-amyloid peptide production in HEK293-APPswe cells.

    abstract：AIM:To identify a small molecule L655,240 as a novel β-secretase (BACE1) inhibitor and to investigate its effects on β-amyloid (Aβ) generation in vitro. METHODS:Fluorescence resonance energy transfer (FRET) was used to characterize the inhibitory effect of L655,240 on BACE1. Surface plasmon resonance (SPR) technology-...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2012.74

    authors： Lu Q,Chen WY,Zhu ZY,Chen J,Xu YC,Kaewpet M,Rukachaisirikul V,Chen LL,Shen X

    更新日期：2012-12-01 00:00:00

  • Enhancement of binding activity of soluble human CD40 to CD40 ligand through incorporation of an isoleucine zipper motif.

    abstract：AIM:To investigate the effect of incorporation of an isoleucine zipper (IZ) motif into CD40 on binding activity of CD40 for the CD40 ligand (CD40L). METHODS:Prokaryotic expression vectors for 2 soluble CD40 derivatives, shCD40His and shCD40IZ containing an IZ domain, were constructed and expressed in Escherichia coli....

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2006.00285.x

    authors： He XH,Xu LH,Liu Y

    更新日期：2006-03-01 00:00:00

  • The artemisinin analog SM934 alleviates dry eye disease in rodent models by regulating TLR4/NF-κB/NLRP3 signaling.

    abstract：:Dry eye disease (DED) is a multifactorial disorder of the tears and ocular surface characterized by manifestations of dryness and irritation. Although the pathogenesis is not fully illuminated, it is recognized that inflammation has a prominent role in the development and deterioration of DED. β-aminoarteether maleate...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-020-0484-5

    authors： Yang FM,Fan D,Yang XQ,Zhu FH,Shao MJ,Li Q,Liu YT,Lin ZM,Cao SQ,Tang W,He SJ,Zuo JP

    更新日期：2020-08-03 00:00:00

  • A cyclic fluctuation model for 24-h ambulatory blood pressure monitoring in Chinese patients with mild to moderate hypertension.

    abstract：AIM:The conventional method for analyzing 24-h ambulatory blood pressure monitoring (24-h ABPM) is insufficient to deal with the large amount of data collected. The aim of this study was to develop a novel cyclic fluctuation model for 24-h ABPM in Chinese patients with mild to moderate hypertension. METHODS:The data w...

    journal_title：Acta pharmacologica Sinica

    pub_type： 临床试验,杂志文章

    doi：10.1038/aps.2013.45

    authors： Sheng YC,Wang K,Xu L,Yang J,He YC,Zheng QS

    更新日期：2013-08-01 00:00:00

  • Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient mice.

    abstract：AIM:Apolipoprotein E (ApoE) plays an important role in the transport and metabolism of lipids. Recent studies show that bone mass is increased in young apoE(-/-) mice. In this study we investigated the bone phenotype and metabolism in aged apoE(-/-) mice. METHODS:Femurs and tibias were collected from 18- and 72-week-o...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2015.95

    authors： Hong W,Xu XY,Qiu ZH,Gao JJ,Wei ZY,Zhen L,Zhang XL,Ye ZB

    更新日期：2015-12-01 00:00:00

  • Oncogenesis of RON receptor tyrosine kinase: a molecular target for malignant epithelial cancers.

    abstract：:Recepteur d'origine nantais (RON) belongs to a subfamily of receptor tyrosine kinases (RTK) with unique expression patterns and biological activities. RON is activated by a serum-derived growth factor macrophage stimulating protein (MSP). The RON gene transcription is essential for embryonic development and critical i...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1111/j.1745-7254.2006.00361.x

    authors： Wang MH,Yao HP,Zhou YQ

    更新日期：2006-06-01 00:00:00

  • Retinol induces morphological alterations and proliferative focus formation through free radical-mediated activation of multiple signaling pathways.

    abstract：AIM:Toxicity of retinol (vitamin A) has been previously associated with apoptosis and/or cell malignant transformation. Thus, we investigated the pathways involved in the induction of proliferation, deformation and proliferative focus formation by retinol in cultured Sertoli cells of rats. METHODS:Sertoli cells were i...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2011.202

    authors： Gelain DP,Pasquali MA,Caregnato FF,Castro MA,Moreira JC

    更新日期：2012-04-01 00:00:00

  • Astragaloside IV attenuates myocardial ischemia/reperfusion injury in rats via inhibition of calcium-sensing receptor-mediated apoptotic signaling pathways.

    abstract：:Astragaloside IV (AsIV) is an active saponin extracted from Astragalus membranaceus, which has shown cardioprotective effects in a number of experimental animals. In this study we investigated the molecular mechanisms by which AsIV attenuated the myocardial ischemia reperfusion (MI/R)-induced injury in vitro and in vi...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-018-0082-y

    authors： Yin B,Hou XW,Lu ML

    更新日期：2019-05-01 00:00:00

  • A simple, quick, and efficient CRISPR/Cas9 genome editing method for human induced pluripotent stem cells.

    abstract：:Induced pluripotent stem cells (iPSCs) have become an essential research platform to study different human diseases once being discovered by Dr. Shinya Yamanaka in 2006. Another breakthrough in biomedical research is the application of CRISPR/Cas9 system for genome editing in mammalian cells. Although numerous studies...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-020-0452-0

    authors： Geng BC,Choi KH,Wang SZ,Chen P,Pan XD,Dong NG,Ko JK,Zhu H

    更新日期：2020-11-01 00:00:00

  • Discrimination analysis of mass spectrometry proteomics for ovarian cancer detection.

    abstract：AIM:A discrimination analysis has been explored for the probabilistic classification of healthy versus ovarian cancer serum samples using proteomics data from mass spectrometry (MS). METHODS:The method employs data normalization, clustering, and a linear discriminant analysis on surface-enhanced laser desorption ioniz...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2008.00861.x

    authors： Hong YJ,Wang XD,Shen D,Zeng S

    更新日期：2008-10-01 00:00:00

  • Pharmacological effects of saw palmetto extract in the lower urinary tract.

    abstract：:Saw palmetto extract (SPE), an extract from the ripe berries of the American dwarf palm, has been widely used as a therapeutic remedy for urinary dysfunction due to benign prostatic hyperplasia (BPH) in Europe. Numerous mechanisms of action have been proposed for SPE, including the inhibition of 5alpha-reductase. Toda...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2009.1

    authors： Suzuki M,Ito Y,Fujino T,Abe M,Umegaki K,Onoue S,Noguchi H,Yamada S

    更新日期：2009-03-01 00:00:00

  • Thrombin induced connective tissue growth factor expression in rat vascular smooth muscle cells via the PAR-1/JNK/AP-1 pathway.

    abstract：AIM:To investigate the signaling pathways involved in thrombin-induced connective tissue growth factor (CTGF) expression in rat vascular smooth muscle cells (VSMCs). METHODS:Experiments were preformed on primary rat aortic smooth muscle cells (RASMCs) and a rat VSMC line (A10). CTGF protein levels were measured using ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2011.178

    authors： Ko WC,Chen BC,Hsu MJ,Tsai CT,Hong CY,Lin CH

    更新日期：2012-01-01 00:00:00

  • Schisandrin B decreases the sensitivity of mitochondria to calcium ion-induced permeability transition and protects against ischemia-reperfusion injury in rat hearts.

    abstract：AIM:In order to elucidate the molecular mechanism underlying the cardioprotection afforded by schisandrin B (Sch B), the effect of Sch B treatment on the sensitivity of mitochondria to Ca2+-stimulated permeability transition (PT) was investigated in rat hearts under normal and ischemia-reperfusion (I-R) conditions. RE...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2007.00614.x

    authors： Chiu PY,Leung HY,Siu AH,Poon MK,Ko KM

    更新日期：2007-10-01 00:00:00

  • Endothelium-derived hyperpolarizing factor mediated relaxations in pig coronary arteries do not involve Gi/o proteins.

    abstract：AIM:Endothelium-dependent relaxations to certain neurohumoral substances are mediated by pertussis toxin-sensitive Gi/o protein. Our experiments were designed to determine the role, if any, of pertussis toxin-sensitive G-proteins in relaxations attributed to endothelium-derived hyperpolarizing factor (EDHF). METHODS:P...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2008.00905.x

    authors： Ng KF,Leung SW,Man RY,Vanhoutte PM

    更新日期：2008-12-01 00:00:00

  • Human umbilical cord blood mesenchymal stem cell transplantation suppresses inflammatory responses and neuronal apoptosis during early stage of focal cerebral ischemia in rabbits.

    abstract：AIM:Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) have been shown to ameliorate cerebral ischemia in animal models. In this study we investigated the effects of hUCB-MSCs on inflammatory responses and neuronal apoptosis during the early stage of focal cerebral ischemia in rabbits. METHODS:Focal cerebra...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2014.9

    authors： Zhu Y,Guan YM,Huang HL,Wang QS

    更新日期：2014-05-01 00:00:00

  • Rapamycin prevents the mutant huntingtin-suppressed GLT-1 expression in cultured astrocytes.

    abstract：AIM:To investigate the effects of rapamycin on glutamate uptake in cultured rat astrocytes expressing N-terminal 552 residues of mutant huntingtin (Htt-552). METHODS:Primary astrocyte cultures were prepared from the cortex of postnatal rat pups. An astrocytes model of Huntington's disease was established using the ast...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2011.162

    authors： Chen LL,Wu JC,Wang LH,Wang J,Qin ZH,Difiglia M,Lin F

    更新日期：2012-03-01 00:00:00

  • Brain IL-1 beta was involved in reserpine-induced behavioral depression in rats.

    abstract：AIM:To investigate the mechanism of brain interleukin-1 beta (IL-1 beta) in reserpine-induced behavioral depression in rats. METHODS:Porsult swim test was used in the measurement of depressive behavior and ELISA was used in measurement of brain IL-1 beta. RESULTS:Intraperitoneal injection of reserpine (0, 4, 6, and 8...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Huang QJ,Jiang H,Hao XL,Minor TR

    更新日期：2004-03-01 00:00:00

  • Effects of Ginkgolide B on action potential and calcium, potassium current in guinea pig ventricular myocytes.

    abstract：AIM:To investigate the effect of Ginkgolide B (GB) on action potential (AP), delayed rectifier potassium current (IK), and L-type calcium current (I(Ca-L)) in guinea pig ventricular myocytes. METHODS:Single ventricular myocytes were isolated by an enzymatic dissociation method. AP, IK, I(Ca-L) were recorded by whole-c...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Qi XY,Zhang ZX,Xu YQ

    更新日期：2004-02-01 00:00:00

  • CYP2E1 mediated isoniazid-induced hepatotoxicity in rats.

    abstract：AIM:To investigate the role of CYP2E1 in isoniazid (INH)-induced hepatotoxicity and the influence of rifampicin (RFP) on INH-induced liver injury. METHODS:Rats were treated with INH alone (100 mg/kg, ip) or co-administered with RFP (100 mg/kg, ig) for 10 d and 21 d. Hepatotoxicity was assayed by plasma enzymes (sALT, ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Yue J,Peng RX,Yang J,Kong R,Liu J

    更新日期：2004-05-01 00:00:00

  • Cardiovascular actions of Radix Stephaniae Tetrandrae: a comparison with its main component, tetrandrine.

    abstract：:A comparison of the cardiovascular actions of the extract of Radix Stephaniae Tetrandrae (RST), the root of a Chinese hero Stephania tetrandra S Moore, in rats with those of tetrandrine (Tet), the best known active component of RST was reviewed. The RST extract inhibits Ca2+ influx into the myocyte and reduces protein...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：

    authors： Wong TM,Wu S,Yu XC,Li HY

    更新日期：2000-12-01 00:00:00

  • Association of ALOX15 gene polymorphisms with obesity-related phenotypes in Chinese nuclear families with male offspring.

    abstract：AIM:Genetic variation in ALOX12, which encoded human 12-lipoxygenase, was found to be associated with fat mass in young Chinese men. The objective of this study was to investigate the relationship between single nucleotide polymorphisms (SNPs) and haplotypes in the ALOX15 gene and obesity-related phenotypes in Chinese ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2011.167

    authors： Ke YH,Xiao WJ,He JW,Zhang H,Yu JB,Hu WW,Gu JM,Gao G,Yue H,Wang C,Hu YQ,Li M,Liu YJ,Fu WZ,Zhang ZL

    更新日期：2012-02-01 00:00:00

  • Mechanisms of antiprostate cancer by gum mastic: NF-kappaB signal as target.

    abstract：AIM:To study the effect of gum mastic, a natural resin, on the proliferation of androgen-independent prostate cancer PC-3 cells, and further investigate the mechanisms involved in this regulatory system, taking nuclear factor kappaB (NF-kappaB) signal as the target. METHODS:3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2007.00536.x

    authors： He ML,Li A,Xu CS,Wang SL,Zhang MJ,Gu H,Yang YQ,Tao HH

    更新日期：2007-03-01 00:00:00

  • Molecular modeling, quantum polarized ligand docking and structure-based 3D-QSAR analysis of the imidazole series as dual AT(1) and ET(A) receptor antagonists.

    abstract：AIM:Both endothelin ETA receptor antagonists and angiotensin AT1 receptor antagonists lower blood pressure in hypertensive patients. A dual AT1 and ETA receptor antagonist may be more efficacious antihypertensive drug. In this study we identified the mode and mechanism of binding of imidazole series of compounds as dua...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2013.129

    authors： Singh KD,Muthusamy K

    更新日期：2013-12-01 00:00:00

  • Epigallocatechin-3-gallate inhibits proliferation and migration of human colon cancer SW620 cells in vitro.

    abstract：AIM:Epigallocatechin-3-gallate (EGCG) is the major polyphenolic constituent in green tea. The aim of this study is to investigate the effects of EGCG on proliferation and migration of the human colon cancer SW620 cells. METHODS:Proliferation and migration of SW620 cells were induced by the protease-activated receptor ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2011.139

    authors： Zhou F,Zhou H,Wang T,Mu Y,Wu B,Guo DL,Zhang XM,Wu Y

    更新日期：2012-01-01 00:00:00

  • Hyposmotic challenge modulates function of L-type calcium channel in rat ventricular myocytes through protein kinase C.

    abstract：AIM:To study the effects and mechanisms by which hyposmotic challenge modulate function of L-type calcium current (I(Ca,L)) in rat ventricular myocytes. METHODS:The whole-cell patch-clamp techniques were used to record I(Ca,L) in rat ventricular myocytes. RESULTS:Hyposmotic challenge(∼220 mosmol/L) induced biphasic c...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2010.112

    authors： Luo AT,Luo HY,Hu XW,Gao LL,Liang HM,Tang M,Hescheler J

    更新日期：2010-11-01 00:00:00

  • Action of aluminum on high voltage-dependent calcium current and its modulation by ginkgolide B.

    abstract：AIM:To investigate the effect of aluminum (Al) on high voltage-dependent calcium current (I(HVA)) and its modulation by ginkgolide B (Gin B). METHODS:The whole-cell, patch-clamp technique was used to record IHVA from acutely isolated hippocampal CA1 pyramydal neurons in rats. RESULTS:Al 0.1 mmol/L (low concentration)...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2005.00073.x

    authors： Chen L,Liu CJ,Tang M,Li A,Hu XW,Du YM,Shen JJ,Lu YL,Heschler J

    更新日期：2005-05-01 00:00:00