当前位置： SCI文献检索 > ACTA PHARMACOLOGICA SINICA期刊下所有文献 > Epigallocatechin-3-gallate inhibits proliferation and migration of human colon cancer SW620 cells in vitro.

Epigallocatechin-3-gallate inhibits proliferation and migration of human colon cancer SW620 cells in vitro.

Abstract：

AIM:Epigallocatechin-3-gallate (EGCG) is the major polyphenolic constituent in green tea. The aim of this study is to investigate the effects of EGCG on proliferation and migration of the human colon cancer SW620 cells. METHODS:Proliferation and migration of SW620 cells were induced by the protease-activated receptor 2-agonist peptide (PAR2-AP, 100 μmol/L) or factor VIIa (10 nmol/L), and analyzed using MTT and Transwell assays, respectively. The cellular cytoskeleton was stained with rhodamine-conjugated phalloidin and examined with a laser scanning confocal fluorescence microscope. The expression of caspase-7, tissue factor (TF) and matrix metalloproteinase (MMP)-9 in the cells was examined using QT-PCR, ELISA and Western blot assays. The activation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) and nuclear factor-kappa B (NF-κB) signaling pathways was analyzed with Western blot. RESULTS:Both PAR2-AP and factor VIIa promoted SW620 cell proliferation and migration, and caused cytoskeleton reorganization (increased filopodia and pseudopodia). Pretreatment with EGCG (25, 50, 75, and 100 μg/mL) dose-dependently blocked the cell proliferation and migration induced by PAR2-AP or factor VIIa. EGCG (100 μg/mL) prevented the cytoskeleton changes induced by PAR2-AP or factor VIIa. EGCG (100 μg/mL) counteracted the down-regulation of caspase-7 expression and up-regulation of TF and MMP-9 expression in the cells treated with PAR2-AP or factor VIIa. Furthermore, it blocked the activation of ERK1/2 and NF-κB (p65/RelA) induced by PAR2-AP or factor VIIa. CONCLUSION:EGCG blocks the proliferation and migration of SW620 cells induced by PAR2-AP and factor VIIa via inhibition of the ERK1/2 and NF-κB pathways. The compound may serve as a preventive and therapeutic agent for colon cancers.

journal_name

Acta Pharmacol Sin

journal_title

Acta pharmacologica Sinica

authors

Zhou F,Zhou H,Wang T,Mu Y,Wu B,Guo DL,Zhang XM,Wu Y

doi

10.1038/aps.2011.139

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

120-6

issue

1

eissn

1671-4083

issn

1745-7254

pii

aps2011139

journal_volume

33

pub_type

杂志文章

相关文献

ACTA PHARMACOLOGICA SINICA文献大全
  • Effect of valsartan and fosinopril on catecholamine-induced cardiac hypertrophy.

    abstract：AIM:To study the influence of angiotensin (Ang) II receptor antagonist (AT1) valsartan and angiotensin-converting enzyme (ACE) inhibitor fosinopril on the cardiac hypertrophy induced by catecholamine. METHODS:A cardiac hypertrophy model was produced by i.p. norepinephrine (NE) 1.5 mg.kg-1.d-1 x 15 d. The animals were ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Xiang Y,Huang J

    更新日期：2000-09-01 00:00:00

  • Smart nanoparticles improve therapy for drug-resistant tumors by overcoming pathophysiological barriers.

    abstract：:The therapeutic outcome of chemotherapy is severely limited by intrinsic or acquired drug resistance, the most common causes of chemotherapy failure. In the past few decades, advancements in nanotechnology have provided alternative strategies for combating tumor drug resistance. Drug-loaded nanoparticles (NPs) have se...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2016.84

    authors： Liu JP,Wang TT,Wang DG,Dong AJ,Li YP,Yu HJ

    更新日期：2017-01-01 00:00:00

  • Caspase-11/4 and gasdermin D-mediated pyroptosis contributes to podocyte injury in mouse diabetic nephropathy.

    abstract：:Diabetic nephropathy (DN) is characterized by sterile inflammation with continuous injury and loss of renal inherent parenchyma cells. Podocyte is an essential early injury target in DN. The injury and loss of podocytes are closely associated with proteinuria, the early symptom of renal injury in DN. However, the exac...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-020-00525-z

    authors： Cheng Q,Pan J,Zhou ZL,Yin F,Xie HY,Chen PP,Li JY,Zheng PQ,Zhou L,Zhang W,Liu J,Lu LM

    更新日期：2020-09-23 00:00:00

  • Epigallocatechin gallate inhibits angiotensin II-induced endothelial barrier dysfunction via inhibition of the p38 MAPK/HSP27 pathway.

    abstract：AIM:To investigate the effect of epigallocatechin gallate (EGCG) on angiotensin II (Ang II)-induced stress fiber formation and hyperpermeability in endothelial cells. METHODS:Human umbilical vein endothelial cells (HUVECs) were treated with Ang II in the absence or presence of EGCG or mitogen-activated protein kinases...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2010.75

    authors： Yang D,Liu J,Tian C,Zeng Y,Zheng YH,Fang Q,Li HH

    更新日期：2010-10-01 00:00:00

  • The transcription factor PBX3 promotes tumor cell growth through transcriptional suppression of the tumor suppressor p53.

    abstract：:Pre-B-cell leukemia transcription factor 3 (PBX3) is a member of the PBX family and contains a highly conserved homologous domain. PBX3 is involved in the progression of gastric cancer, colorectal cancer, and prostate cancer; however, the detailed mechanism by which it promotes tumor growth remains to be elucidated. H...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-020-00599-9

    authors： Li WF,Herkilini A,Tang Y,Huang P,Song GB,Miyagishi M,Kasim V,Wu SR

    更新日期：2021-02-01 00:00:00

  • Astragaloside IV attenuates myocardial ischemia/reperfusion injury in rats via inhibition of calcium-sensing receptor-mediated apoptotic signaling pathways.

    abstract：:Astragaloside IV (AsIV) is an active saponin extracted from Astragalus membranaceus, which has shown cardioprotective effects in a number of experimental animals. In this study we investigated the molecular mechanisms by which AsIV attenuated the myocardial ischemia reperfusion (MI/R)-induced injury in vitro and in vi...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-018-0082-y

    authors： Yin B,Hou XW,Lu ML

    更新日期：2019-05-01 00:00:00

  • Angiotensin II type 2 receptor gene polymorphisms and essential hypertension.

    abstract：AIM:To identify the genetic variants of angiotensin II type 2 receptor (AT2R) gene in a Chinese population and to determine whether the AT2R gene polymorphisms are associated with essential hypertension (EH). METHODS:The detection of single nucleotide polymorphisms (SNPs) was performed in 19 subjects by a direct DNA s...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Zhang Y,Zhang KX,Wang GL,Huang W,Zhu DL

    更新日期：2003-11-01 00:00:00

  • Production of neutralizing monoclonal antibody against human vascular endothelial growth factor receptor II.

    abstract：AIM:To prepare neutralizing monoclonal antibody (mAb) against extracellular immunoglobulin (Ig)-like domain III of vascular endothelial growth factor receptor KDR and study its biological activity. METHODS:Soluble KDR Ig domain III (KDR-III) fusion protein was expressed in E Coli and purified from the bacterial peripl...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Li R,Xiong DS,Shao XF,Liu J,Xu YF,Xu YS,Liu HZ,Zhu ZP,Yang CZ

    更新日期：2004-10-01 00:00:00

  • A novel derivative of valepotriate inhibits the PI3K/AKT pathway and causes Noxa-dependent apoptosis in human pancreatic cancer cells.

    abstract：:Natural compound valepotriate exhibits inhibitory activity against a number of cancers, but the effect of valepotriate against pancreatic cancer is unclear, and the structure-activity relationship of valepotriate has not been characterized. In this study, we performed a structure-based similarity search and found 16 h...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-019-0354-1

    authors： Yan YY,Shi KY,Teng F,Chen J,Che JX,Dong XW,Lin NM,Zhang B

    更新日期：2020-06-01 00:00:00

  • Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract.

    abstract：AIM:To study the protein and mRNA expressions of pro-inflammatory and anti-inflammatory cytokines in the brain of rats with atherosclerosis (AS) and the effects of Ginkgo biloba extract (GbE) on expressions of cytokines. METHODS:The experimental model of AS in rats were established by intraperitioneal injection of vit...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2005.00106.x

    authors： Jiao YB,Rui YC,Li TJ,Yang PY,Qiu Y

    更新日期：2005-07-01 00:00:00

  • The effect of pinacidil on postshock activation and ventricular defibrillation threshold in canine hearts.

    abstract：AIM:To determine the postshock activation patterns with both successful and failed shocks in a canine model of ventricular fibrillation, and whether piniacidil, an early after-depolarization (EAD) inhibitor, altered the defibrillation threshold (DFT) and postshock activation patterns. METHODS:In 6 beagles, a basket ca...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2012.96

    authors： Jin Q,Zhang N,Zhou J,Lin CJ,Pang Y,Gu G,Shen WF,Wu LQ

    更新日期：2012-12-01 00:00:00

  • Dysfunction of myocardial taurine transport and effect of taurine supplement in rats with isoproterenol-induced myocardial injury.

    abstract：AIM:To study the alterations of myocardial taurine transport function, taurine transporter (TAUT), and cysteine sulfinate decarboxylase (CSD) mRNA as well as effect of exogenous taurine in rats with isoproterenol (ISO)-induced cardiomegaly. METHODS:[3H]-Taurine uptake and release on myocardium were determined. Binding...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Shi YR,Bu DF,Qi YF,Gao L,Jiang HF,Pang YZ,Tang CS,Du JB

    更新日期：2002-10-01 00:00:00

  • TSPO ligands prevent the proliferation of vascular smooth muscle cells and attenuate neointima formation through AMPK activation.

    abstract：:Abnormal growth of the intimal layer of blood vessels (neointima formation) contributes to the progression of atherosclerosis and in-stent restenosis. Recent evidence shows that the 18-kDa translocator protein (TSPO), a mitochondrial membrane protein, is involved in diverse cardiovascular diseases. In this study we in...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-019-0293-x

    authors： Wu LP,Gong ZF,Wang H,Zhou ZS,Zhang MM,Liu C,Ren HM,Yang J,Han Y,Zeng CY

    更新日期：2020-01-01 00:00:00

  • Protective effect of polypeptide from Chlamys farreri on hairless mice damaged by ultraviolet A.

    abstract：AIM:To study the protective effect of the polypeptide isolated from Chlamys farreri (PCF) on hairless mice skin damaged by ultraviolet A. METHODS:Enzymes and malondialdehyde (MDA) were determined by biochemical methods; the expressions of Bcl-2 protein and NOS protein were examined by immunohistochemical technique. Th...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Wang CB,Yao RY,Liu ZT,Zhong WZ,Liu XP,Wang YJ

    更新日期：2002-09-01 00:00:00

  • W2476 ameliorates β-cell dysfunction and exerts therapeutic effects in mouse models of diabetes via modulation of the thioredoxin-interacting protein signaling pathway.

    abstract：:Recent evidence shows that high glucose levels recruit carbohydrate response element-binding protein, which binds the promoter of thioredoxin-interacting protein (txnip), thereby regulating its expression in β-cells. Overexpression of txnip not only induces β-cell apoptosis but also reduces insulin production. Thus, t...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2017.15

    authors： Li T,Lin GY,Zhong L,Zhou Y,Wang J,Zhu Y,Feng Y,Cai XQ,Liu Q,Nosjean O,Boutin JA,Renard P,Yang DH,Wang MW

    更新日期：2017-07-01 00:00:00

  • Type I natural killer T cells: naturally born for fighting.

    abstract：:Type capital I, Ukrainian natural killer T cells (NKT cells), a subset of CD1d-restricted T cells with invariant Valphabeta TCR, are characterized by prompt production of large amounts of Th1 and/or Th2 cytokines upon primary stimulation through the TCR complex. The rapid release of cytokines implies that type capital...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2010.119

    authors： Tan JQ,Xiao W,Wang L,He YL

    更新日期：2010-09-01 00:00:00

  • Neuronal nicotinic acetylcholine receptors are important targets for alcohol reward and dependence.

    abstract：:Neuronal nicotinic acetylcholine receptors are important targets for alcohol reward and dependence. Alcoholism is a serious public health problem and has been identified as the third major cause of preventable mortality in the world. Worldwide, about 2 billion people consume alcohol, with 76.3 million having diagnosab...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2013.181

    authors： Wu J,Gao M,Taylor DH

    更新日期：2014-03-01 00:00:00

  • Effects of (-)stepholidine in animal models for schizophrenia.

    abstract：AIM:(-)Stepholidine (SPD) is an active ingredient of the Chinese herb Stephania intermedia, which binds to the dopamine D(1) and D(2) like receptors. Biochemical, electrophysiological and behavioural experiments have provided strong evidence that SPD is both a D(1) and a D(2) antagonist, which could make SPD a unique a...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2006.00365.x

    authors： Ellenbroek BA,Zhang XX,Jin GZ

    更新日期：2006-09-01 00:00:00

  • Effects of Ginkgolide B on action potential and calcium, potassium current in guinea pig ventricular myocytes.

    abstract：AIM:To investigate the effect of Ginkgolide B (GB) on action potential (AP), delayed rectifier potassium current (IK), and L-type calcium current (I(Ca-L)) in guinea pig ventricular myocytes. METHODS:Single ventricular myocytes were isolated by an enzymatic dissociation method. AP, IK, I(Ca-L) were recorded by whole-c...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Qi XY,Zhang ZX,Xu YQ

    更新日期：2004-02-01 00:00:00

  • Doxorubicin-induced vasomotion and [Ca(2+)](i) elevation in vascular smooth muscle cells from C57BL/6 mice.

    abstract：AIM:To explore the action of doxorubicin on vascular smooth muscle cells. METHODS:Isometric tension of denuded or intact thoracic aortic vessels was recorded and [Ca(2+)](i) in isolated aortic smooth muscle cells was measured by using Fluo-3. RESULTS:Doxorubicin induced phasic and tonic contractions in denuded vessel...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2009.145

    authors： Shen B,Ye CL,Ye KH,Zhuang L,Jiang JH

    更新日期：2009-11-01 00:00:00

  • Monocarbonyl curcumin analog A2 potently inhibits angiogenesis by inducing ROS-dependent endothelial cell death.

    abstract：:Excessive and abnormal vessel growth plays a critical role in the pathogenesis of many diseases, such as cancer. Angiogenesis is one of the hallmarks of cancer growth, invasion, and metastasis. Discovery of novel antiangiogenic agents would provide new insights into the mechanisms of angiogenesis, as well as potential...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-019-0224-x

    authors： Liu B,Cui LS,Zhou B,Zhang LL,Liu ZH,Zhang L

    更新日期：2019-11-01 00:00:00

  • Effect of cholecystokinin octapeptide on diacylglycerol-PKC signaling pathway in rat pulmonary interstitial macrophages stimulated by lipopolysaccharide.

    abstract：AIM:To investigate the effect of cholecystokinin octapeptide (CCK-8) on the diacylglycerol-protein kinase C (DAG-PKC) signaling pathway in rat pulmonary interstitial macrophages (PIM) stimulated by lipopolysaccaride (LPS). METHODS:The PIM from rat lung tissues were isolated using the collagenase digestion method combi...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2005.00217.x

    authors： Xu SJ,Gao WJ,Cong B,Ma CL,Li SJ,Ling YL,Gu ZY,Yao YX

    更新日期：2005-12-01 00:00:00

  • Propofol affects mouse embryonic fibroblast survival and proliferation in vitro via ATG5- and calcium-dependent regulation of autophagy.

    abstract：:Propofol is a commonly used intravenous anesthetic agent, which has been found to affect cell survival and proliferation especially in early life. Our previous studies show that propofol-induced neurodegeneration and neurogenesis are closely associated with cell autophagy. In the present study we explored the roles of...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-019-0303-z

    authors： Xu ZD,Wang Y,Liang G,Liu ZQ,Ma WH,Chu CT,Wei HF

    更新日期：2020-03-01 00:00:00

  • Microbial transformation of naproxen by Cunninghamella species.

    abstract：AIM:The metabolites of naproxen produced by Cunninghamella species were isolated and identified, and further to compare the similarities between microbial transformation and mammalian metabolism. METHODS:Naproxen was transformed by three strains of Cunninghammella species (Cunninghamella blakeslesna AS 3.153, Cunningh...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Zhong DF,Sun L,Liu L,Huang HH

    更新日期：2003-05-01 00:00:00

  • Effect of tetrandrine on free intracellular calcium in cultured calf basilar artery smooth muscle cells.

    abstract：AIM:To study the effects of tetrandrine (Tet) on extracellular Ca2+ influx and intracellular Ca2+ release in cultured calf basilar artery smooth muscle cells. METHODS:Free intracellular calcium was examined by a system of measurement of AR-CM-MIC, using Fura 2-AM as a fluorescent indicator. RESULTS:In the presence of...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Wang B,Xiao JG

    更新日期：2002-12-01 00:00:00

  • Proteome reference map and regulation network of neonatal rat cardiomyocyte.

    abstract：AIM:To study and establish a proteome reference map and regulation network of neonatal rat cardiomyocyte. METHODS:Cultured cardiomyocytes of neonatal rats were used. All proteins expressed in the cardiomyocytes were separated and identified by two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assist...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2011.86

    authors： Li ZJ,Liu N,Han QD,Zhang YY

    更新日期：2011-09-01 00:00:00

  • Apoptosis initiated by carbon tetrachloride in mitochondria of rat primary cultured hepatocytes.

    abstract：AIM:To investigate the mitochondria-initiated apoptosis pathway involved in Carbon tetrachloride (CCl4) hepatotoxicity in vitro. METHODS:Several cytotoxicity endpoints, including WST-8 metabolism, lactate dehydrogenase leakage and morphological changes, were examined. The 5,5'-dithio-bis(2-nitrobenzoic acid) reaction ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2005.00143.x

    authors： Cai Y,Gong LK,Qi XM,Li XH,Ren J

    更新日期：2005-08-01 00:00:00

  • Metformin modulates insulin post-receptor signaling transduction in chronically insulin-treated Hep G2 cells.

    abstract：AIM:To study the effect of chronic insulin treatment on insulin post-receptor signaling transduction and whether the effects of metformin are transmitted throughout the cascade of insulin signaling intermediates in a human hepatoma cell line (Hep G2). METHODS:Hep G2 cells were incubated in serum free media containing ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Yuan L,Ziegler R,Hamann A

    更新日期：2003-01-01 00:00:00

  • Changes of phospholipase D activity of rat peritoneal mast cells in degranulation.

    abstract：AIM:To study the changes of phospholipase D (PLD) activity of actively sensitized rat peritoneal mast cells (RPMC) in degranulation. METHODS:Degranulation of RPMC was determined by measurement of beta-hexosaminidase release. PLD activity assay was carried out by measurement of PLD product, choline, with chemiluminesce...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Lu YB,Wu M,Zhou HL

    更新日期：2004-01-01 00:00:00

  • Correlative changes of interferon-gamma and interleukin-4 between cortical layer and pulmonary airway of sensitized rats.

    abstract：AIM:To explore correlation of T helper 1 (Th1) and Th2 cytokine between the cortical layer and pulmonary airway in ovalbumin-induced rat asthma model. METHODS:Aerosol antigen-induced changes of inflammation in bronchoalveolar lavage fluids (BALF) and pulmonary histologic section in sensitized rats were investigated. C...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Xie QM,Chen JQ,Shen WH,Bian RL

    更新日期：2002-03-01 00:00:00