Propofol affects mouse embryonic fibroblast survival and proliferation in vitro via ATG5- and calcium-dependent regulation of autophagy.

abstract：AIM:Our previous study demonstrated an siRNA-mediated, allele-specific silencing of mutant genes that cause amyotrophic lateral sclerosis. To improve siRNA design for better therapeutic use of RNA interference, we systematically tested the base-pairing mismatch strategy in the design of asymmetric siRNA. METHODS:A nat...

journal_title：Acta pharmacologica Sinica

authors： Geng CM,Ding HL

更新日期：2008-02-01 00:00:00

abstract：:Parkinson's disease (PD) is a common neurodegenerative disease characterized by motor impairment and progressive loss of dopamine (DA) neurons. At present, the acute application of neurotoxic drugs such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 6-hydroxydopamine (6-OHDA) are commonly used to simulate ...

journal_title：Acta pharmacologica Sinica

authors： Guo CH,Cao T,Zheng LT,Waddington JL,Zhen XC

更新日期：2020-04-01 00:00:00

abstract：:The intercellular communication between leukemia cells and bone marrow mesenchymal stem cells (BM-MSCs) plays more important role in chronic myeloid leukemia (CML) than we previously understood. Recently, we found that microvesicles released from human leukemia cell line K562 (K562-MVs) containing BCR-ABL1 mRNA malign...

journal_title：Acta pharmacologica Sinica

authors： Fu FF,Zhu XJ,Wang HX,Zhang LM,Yuan GL,Chen ZC,Li QB

更新日期：2017-11-01 00:00:00

abstract：AIM:FTY720 is a new synthetic immunosuppressive agent which has a unique mechanism of action and induces long-term graft acceptance in rat and dog allotransplantation as prophylactic administration. The present study investigated whether FTY720 was able to rescue ongoing acute rejection of solid organ transplants in a ...

journal_title：Acta pharmacologica Sinica

authors： Wang MH,Milekhin V,Zhang H,Huang HZ

更新日期：2003-09-01 00:00:00

abstract：AIM:The aim of the study was to evaluate the outcome of adenovirus-mediated p27(KIP1) (Ad-p27) expression on wound healing after filtration surgery and to investigate the inhibition of cell proliferation induced by Ad-p27. METHODS:We constructed the adenovirus recombinant vector Ad-p27 and administered it to a rabbit ...

journal_title：Acta pharmacologica Sinica

authors： Yang JG,Sun NX,Cui LJ,Wang XH,Feng ZH

更新日期：2009-04-01 00:00:00

abstract：:Contact inhibition and its disruption of vascular smooth muscle cells (VSMCs) are important cellular events in vascular diseases. But the underlying molecular mechanisms are unclear. In this study we investigated the roles of microRNAs (miRNAs) in the contact inhibition and its disruption of VSMCs and the molecular me...

journal_title：Acta pharmacologica Sinica

authors： Sun YY,Qin SS,Cheng YH,Wang CY,Liu XJ,Liu Y,Zhang XL,Zhang W,Zhan JX,Shao S,Bian WH,Luo BH,Lu DF,Yang J,Wang CH,Zhang CX

更新日期：2018-05-01 00:00:00

abstract：AIM:To determine whether deguelin can regulate the expression of nuclear factor kappa B (NF-kappaB) binding protein (IkappaBalpha) in U937 human leukemia cells and Raji human B lymphoma cells. METHODS:The localization of IkappaBalpha protein was investigated by using an immunofluorescence method. The expression of Ika...

journal_title：Acta pharmacologica Sinica

authors： Chen WH,Chen Y,Cui GH

更新日期：2006-04-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) have become an essential research platform to study different human diseases once being discovered by Dr. Shinya Yamanaka in 2006. Another breakthrough in biomedical research is the application of CRISPR/Cas9 system for genome editing in mammalian cells. Although numerous studies...

journal_title：Acta pharmacologica Sinica

authors： Geng BC,Choi KH,Wang SZ,Chen P,Pan XD,Dong NG,Ko JK,Zhu H

更新日期：2020-11-01 00:00:00

abstract：AIM:To investigate the effects of angiotensin II (Ang II) on corticotropin-releasing hormone (CRH) and argipressin (AVP) release from median eminence (ME) of hypothalamus during acute hypoxia in rats. METHODS:Simulated hypoxia was performed in a hypobaric chamber. CRH and AVP were determined by radioimmunoassay (RIA)....

journal_title：Acta pharmacologica Sinica

authors： Wu Y,Du JZ

更新日期：2000-11-01 00:00:00

abstract：AIM:To study the changes of phospholipase D (PLD) activity of actively sensitized rat peritoneal mast cells (RPMC) in degranulation. METHODS:Degranulation of RPMC was determined by measurement of beta-hexosaminidase release. PLD activity assay was carried out by measurement of PLD product, choline, with chemiluminesce...

journal_title：Acta pharmacologica Sinica

authors： Lu YB,Wu M,Zhou HL

更新日期：2004-01-01 00:00:00

abstract：AIM:To study whether etodolac enantiomers have pharmacokinetic difference after oral administration. METHODS:Fourteen rats, divided into two groups randomly, were orally given S-(+)- or R-(-)-etodolac at a single dose of 20 mg/kg, respectively. Blood samples were collected before and at 5, 10, 20, 30 min and 1, 3, 6, ...

journal_title：Acta pharmacologica Sinica

authors： Shi JM,Lai SG,Xu CJ,Duan GL,Li D

更新日期：2004-08-01 00:00:00

abstract：AIM:To observe the effects of tripcholorolide (T4) on inflammatory reaction of mouse alveolar macrophages. METHODS:RT-PCR was used to investigate tumor necrosis factor alpha (TNF alpha), interleukin-1 beta (IL-1 beta), IL-6, IL-10, and inducible nitric-oxide synthase (iNOS) mRNA expression in alveolar macrophages afte...

journal_title：Acta pharmacologica Sinica

authors： Qiu HB,Yang Y,Zhou SX

更新日期：2000-12-01 00:00:00

abstract：AIM:To explore the effects of glutamine on growth and apoptosis of hepatoma cells. METHODS:Mice inoculated with hepatoma cell (H22) suspension subcutaneously at right axilla were orally administered with glutamine (GLN) solution. Human hepatoma cell culture (SMMC-7721) was treated with different concentrations of GLN ...

journal_title：Acta pharmacologica Sinica

authors： Liu SL,Shi DY,Shen ZH,Wu YD

更新日期：2000-07-01 00:00:00

abstract：:Terpene lactones are a class of bioactive constituents of standardized preparations of Ginkgo biloba leaf extract, extensively used as add-on therapies in patients with ischemic cardiovascular and cerebrovascular diseases. This investigation evaluated human pharmacokinetics of ginkgo terpene lactones and impact of the...

journal_title：Acta pharmacologica Sinica

authors： Liu XW,Yang JL,Niu W,Jia WW,Olaleye OE,Wen Q,Duan XN,Huang YH,Wang FQ,Du FF,Zhong CC,Li YF,Xu F,Gao Q,Li L,Li C

更新日期：2018-12-01 00:00:00

abstract：:Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as neural apoptosis regulated convertase (NARC1), is a key modulator of cholesterol metabolism. PCSK9 increases the serum concentration of low-density lipoprotein cholesterol by escorting low-density lipoprotein receptors (LDLRs) from the membrane of he...

journal_title：Acta pharmacologica Sinica

authors： He NY,Li Q,Wu CY,Ren Z,Gao Y,Pan LH,Wang MM,Wen HY,Jiang ZS,Tang ZH,Liu LS

更新日期：2017-03-01 00:00:00

abstract：AIM:To acquire the specific RNA aptamers inhibiting human red blood cell (RBC) acetylcholinesterase (AChE). METHODS:Systematic evolution of ligands by exponential enrichment (SELEX) aptamer against human red blood cell membrane AChE was selected by microtiter plate method in vitro. The specifity binding to AChE was de...

journal_title：Acta pharmacologica Sinica

authors： Zhang XM,Shao NS,Chi MG,Sun MJ

更新日期：2003-07-01 00:00:00

abstract：AIM:To study the protein and mRNA expressions of pro-inflammatory and anti-inflammatory cytokines in the brain of rats with atherosclerosis (AS) and the effects of Ginkgo biloba extract (GbE) on expressions of cytokines. METHODS:The experimental model of AS in rats were established by intraperitioneal injection of vit...

journal_title：Acta pharmacologica Sinica

authors： Jiao YB,Rui YC,Li TJ,Yang PY,Qiu Y

更新日期：2005-07-01 00:00:00

abstract：AIM:To investigate the protective effects of hydrogen sulfide (H2S) against inflammation, oxidative stress and apoptosis in a rat model of resuscitated hemorrhagic shock. METHODS:Hemorrhagic shock was induced in adult male SD rats by drawing blood from the femoral artery for 10 min. The mean arterial pressure was main...

journal_title：Acta pharmacologica Sinica

authors： Xu DQ,Gao C,Niu W,Li Y,Wang YX,Gao CJ,Ding Q,Yao LN,Chai W,Li ZC

更新日期：2013-12-01 00:00:00

abstract：:Recent evidence shows that raddeanin A (RA), an oleanane-type triterpenoid saponin extracted from Anemone raddeana Regel, exerts remarkable cytotoxicity against cancer cells in vitro and in vivo. In addition, RA has also been found to activate autophagy in human gastric cancer cells. In this study, we investigated the...

journal_title：Acta pharmacologica Sinica

authors： Guan YD,Jiang SL,Yu P,Wen M,Zhang Y,Xiao SS,Xu XJ,Cheng Y

更新日期：2018-04-01 00:00:00

abstract：AIM:To discover novel cell division cycle 25 (CDC25) B inhibitors and elucidate the mechanisms of inhibition in cancer cells. METHODS:Cell growth inhibition was detected by MTT assay, the cell cycle was analyzed by flow cytometry, and protein expression and phosphorylation was examined by Western blot analysis. RESUL...

journal_title：Acta pharmacologica Sinica

authors： Zhou YB,Feng X,Wang LN,Du JQ,Zhou YY,Yu HP,Zang Y,Li JY,Li J

更新日期：2009-09-01 00:00:00

abstract：:The hypnotic agent propofol is effective for the induction and maintenance of anesthesia. However, recent studies have shown that propofol administration is related to arrhythmias. Propofol displays both pro- and anti-arrhythmic effects in a concentration-dependent manner. Data indicate that propofol can convert supra...

journal_title：Acta pharmacologica Sinica

authors： Liu Q,Kong AL,Chen R,Qian C,Liu SW,Sun BG,Wang LX,Song LS,Hong J

更新日期：2011-06-01 00:00:00

abstract：AIM:G protein-coupled inwardly rectifying potassium channels (GIRK) are important for neuronal signaling and membrane excitability. In the present study, we intend to find whether GIRK channels express functionally in adult rat dorsal root ganglion (DRG) neurons. METHODS:We used RT-PCR to detect mRNA for 4 subunits of...

journal_title：Acta pharmacologica Sinica

authors： Gao XF,Zhang HL,You ZD,Lu CL,He C

更新日期：2007-02-01 00:00:00

abstract：AIM:To examine the effects of anisomycin on glioma cells and the related mechanisms in vitro. METHODS:The U251 and U87 human glioblastoma cell lines were tested. The growth of the cells was analyzed using a CCK-8 cell viability assay. Apoptosis was detected using a flow cytometry assay. The expression of proteins and ...

journal_title：Acta pharmacologica Sinica

authors： Li JY,Huang JY,Li M,Zhang H,Xing B,Chen G,Wei D,Gu PY,Hu WX

更新日期：2012-07-01 00:00:00

abstract：AIM:To investigate the protective effects of prostaglandin E(1) (PGE(1)) against H(2)O(2)-induced oxidative damage on human umbilical vein endothelial cells (HUVECs). METHODS:HUVECs were pretreated with PGE(1) (0.25, 0.50, and 1.00 micromol/L) for 24 h and exposed to H(2)O(2) (200 micromol/L) for 12 h, and cell viabil...

journal_title：Acta pharmacologica Sinica

authors： Fang WT,Li HJ,Zhou LS

更新日期：2010-04-01 00:00:00

abstract：:Volatile anesthetics improve postischemic cardiac function and reduce infarction even when administered for only a brief time at the onset of reperfusion. A recent study showed that sevoflurane postconditioning (SPC) attenuated myocardial reperfusion injury, but the underlying mechanisms remain unclear. In this study,...

journal_title：Acta pharmacologica Sinica

authors： Qiao SG,Sun Y,Sun B,Wang A,Qiu J,Hong L,An JZ,Wang C,Zhang HL

更新日期：2019-01-01 00:00:00

abstract：AIM:To Study the interaction of macrophage colony-stimulating factor (M-CSF) and interleukin-10 (IL-10) in productions of IL-12 and IL-18 and expressions of CD14, CD23, and CD64 by human monocytes. METHODS:Purified adherent human monocytes were cultured with M-CSF or IL-10 alone, or with M-CSF+IL-10 and 2-3d later, th...

journal_title：Acta pharmacologica Sinica

authors： Ji XH,Yao T,Qin JC,Wang SK,Wang HJ,Yao K

更新日期：2004-10-01 00:00:00

abstract：:Central nervous system (CNS) disorders represent a broad spectrum of brain ailments with short- and long-term disabilities, and nanomedicine-based approaches provide a new therapeutic approach to treating CNS disorders. A variety of potential drugs have been discovered to treat several neuronal disorders; however, the...

journal_title：Acta pharmacologica Sinica

authors： Hanif S,Muhammad P,Chesworth R,Rehman FU,Qian RJ,Zheng M,Shi BY

更新日期：2020-07-01 00:00:00

abstract：:The signal transducer and activator of transcription 3 (STAT3) plays a critical role in platelet functions. This study sought to understand the effects of the STAT3 inhibitor SC99 on platelet activation and aggregation. Immunoblotting assays were applied to measure the effects of SC99 on the STAT3 signaling pathway. A...

journal_title：Acta pharmacologica Sinica

authors： Xu Z,Xu YJ,Hao YN,Ren LJ,Zhang ZB,Xu X,Cao BY,Dai KS,Zhu L,Fang Q,Kong Y,Mao XL

更新日期：2017-05-01 00:00:00

abstract：:Liver fibrosis is excessive accumulation of extracellular matrix proteins that results from various chronic liver diseases. Hepatic stellate cells (HSCs) play an essential role in the pathogenesis of liver fibrosis. Danggui Buxue Tang (DBT) is a classic formula of Chinese traditional medicine. We previously showed tha...

journal_title：Acta pharmacologica Sinica

authors： Guo T,Liu ZL,Zhao Q,Zhao ZM,Liu CH

更新日期：2018-09-01 00:00:00

abstract：AIM:Aquaporins (AQPs) are the water-channels that play important roles in brain water homeostasis and in cerebral edema induced by brain injury. In this study we investigated the relationship between AQPs and a neuroprotective agent curcumin that was effective in the treatment of brain edema in mice with intracerebral ...

journal_title：Acta pharmacologica Sinica

authors： Wang BF,Cui ZW,Zhong ZH,Sun YH,Sun QF,Yang GY,Bian LG

更新日期：2015-08-01 00:00:00