Abstract:
BACKGROUND:Recently, 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is being widely used in cancer therapy owing to the tumor-specific accumulation of photosensitizing protoporphyrin IX (PpIX) after the administration of ALA. In the present study, by focusing on genes involved in the porphyrin biosynthesis pathway, we aimed to explore biomarkers that are predictive for the efficacy of ALA-PDT. METHODS:We used five lines of human gastric cancer cells to measure the ALA-based photocytotoxicity. ALA-induced production of PpIX in cancer cells was quantified by fluorescence spectrophotometry. To examine the potential involvement of PEPT1 and ABCG2 in the ALA-PDT sensitivity, stable cell lines overexpressing PEPT1 were established and ABCG2-specific siRNA used. RESULTS:We observed that three cell lines were photosensitive, whereas the other two cell lines were resistant to ALA-based photocytotoxicity. The ALA-based photocytotoxicity was found to be well correlated with intracellular PpIX levels, which suggests that certain enzymes and/or transporters involved in ALA-induced PpIX production are critical determinants. We found that high expression of the peptide transporter PEPT1 (ALA influx transporter) and low expression of the ATP-binding cassette transporter ABCG2 (porphyrin efflux transporter) determined ALA-induced PpIX production and cellular photosensitivity in vitro. CONCLUSION:PEPT1 and ABCG2 are key players in regulating intracellular PpIX levels and determining the efficacy of ALA-based photocytotoxicity against gastric cancer cells in vitro. Evaluation of the expression levels of PEPT1 and ABCG2 genes could be useful to predict the efficacy of ALA-PDT. Primers specific to those target genes are practical and useful biomarkers for predicting the photo-sensitivity to ALA-PDT.
journal_name
Photodiagnosis Photodyn Therjournal_title
Photodiagnosis and photodynamic therapyauthors
Hagiya Y,Endo Y,Yonemura Y,Takahashi K,Ishizuka M,Abe F,Tanaka T,Okura I,Nakajima M,Ishikawa T,Ogura Sdoi
10.1016/j.pdpdt.2011.12.004subject
Has Abstractpub_date
2012-09-01 00:00:00pages
204-14issue
3eissn
1572-1000issn
1873-1597pii
S1572-1000(11)00457-1journal_volume
9pub_type
杂志文章abstract:BACKGROUND:The objective of this paper was to verify if the oral administration of δ-aminolevulinic acid (ALA) in animals with prostate tumor can increase the sensitivity of cancer diagnosis by protoporphyrin IX blood autofluorescence. In this study, the autofluorescence of blood porphyrin was analyzed using fluorescen...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2010.12.006
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:Erosive oral lichen planus (EOLP) poses a substantial risk of malignant transformation into squamous cell cancer. The absence of established treatment gives way to alternative therapeutic strategies, including photodynamic therapy. The aim of the study was to evaluate the efficacy of PDT in the treatment of ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2017.01.178
更新日期:2017-06-01 00:00:00
abstract::Photodynamic therapy (PDT) has emerged as an effective and minimally invasive treatment option for several diseases, including some forms of cancer. However, several drawbacks of the approved photosensitizers (PS), such as insufficient light absorption at therapeutically relevant wavelengths hampered the clinical effe...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,评审
doi:10.1016/j.pdpdt.2015.07.001
更新日期:2016-03-01 00:00:00
abstract::Port wine stain (PWS) birthmarks are congenital vascular malformations characterized by ectatic capillaries in the papillary layer of the dermis. They usually appear at birth and tend to become darker and thicker with age. Cosmetic and pathological changes can cause great depression in all ages. Laser-mediated selecti...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,评审
doi:10.1016/j.pdpdt.2009.08.001
更新日期:2009-09-01 00:00:00
abstract::Extramedullary plasmacytomas (EMP) are rare plasma cell neoplasms that are mostly located in the mucosal surfaces of the upper aerodigestive tract. The standard treatment is radiotherapy (RT), with surgical resection reserved as salvage procedure. In this report a patient with a EMP, located in the nasopharynx and ref...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2011.03.340
更新日期:2011-09-01 00:00:00
abstract:SIGNIFICANCE:The estimation of tissue oxygenation is vital in the diagnosis and therapeutic evaluation of a huge assortment of diseases. However, the hyperspectral (HS) imaging system is a rising innovation that can be utilized to build a highly sensitive, non-invasive, and tissue hemoglobin immersion map. OBJECTIVE:A...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2021.102190
更新日期:2021-01-25 00:00:00
abstract::Perifolliculitis capitis abscedens et suffodiens (PCAS), also known as dissecting cellulitis of the scalp (DCS), is a rare, chronic, suppurative inflammatory disease of the scalp which may evolve into cicatricial alopecia. Its refractory nature has a detrimental impact on patients' quality of life. Here, we report fiv...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101660
更新日期:2020-06-01 00:00:00
abstract:AIM:The aim of the present clinical trial was to evaluate the efficacy of photodynamic therapy (PDT) as an adjunct to mechanical debridement (MD) in the treatment of severe peri-implantitis (PI) with abscess. MATERIALS AND METHODS:Selected individuals with severe PI were divided into two groups: Group A: received meth...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101750
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Nanosecond pulsed electric field (nsPEF) treatment is a new anti-cancer strategy with ultrashort pulse duration and high intensity of electric fields. The application of nsPEFs affects all intra- and extracellular membranes and independently initiates the process of apoptosis within cancer cells, leading the...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2015.11.002
更新日期:2015-12-01 00:00:00
abstract:BACKGROUND:Condyloma acuminatum is a highly infectious disease caused by the human papillomavirus. Previous studies have shown that 5-aminolevulinic acid photodynamic therapy can inhibit proliferation of condyloma acuminatum keratinocytes. However, the effect of 5-aminolevulinic acid photodynamic therapy on condyloma a...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.07.018
更新日期:2019-12-01 00:00:00
abstract::A case of recalcitrant dissecting cellulitis of the scalp (DCS) in a 23-year-old male patient was treated with 4 times of topical ALA-PDT at intervals of 10-15 days. The patient responded well without any unbearable adverse effects and presented satisfactory effects. Before applying this therapy, the patient and his d...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.10.001
更新日期:2018-12-01 00:00:00
abstract::We report the case of a patient with recurrence of follicular carcinoma of the thyroid 8 years after surgical resection followed by external beam radiotherapy and radio-iodine treatment. The patient was treated by endoscopic photodynamic therapy (PDT) with complete endoscopic response after 12 months with good symptom...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/S1572-1000(04)00016-X
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND/AIM:Laser-based photodynamic endoscopic diagnosis (LPDED) is a type of endoscopic diagnosis that uses the fluorescence caused by the photochemical reaction that occurs when a fluorescent substance is irradiated by a light of a specific wavelength. Although 5-aminolevulinic acid (5-ALA) can detect early gastr...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.02.005
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:In this study, the photosensitivity of 5-aminolevulinic acid (5-ALA) conjugated gold nanoparticles (conjugate) has been investigated on the MeL-Rm cell line. METHODS:After culture and proliferation of the Mel-Rm cell line, cell toxicity and the suitable cell incubation time of the conjugate were determined....
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2013.03.010
更新日期:2013-12-01 00:00:00
abstract:INTRODUCTION:The aim of the present study was to evaluate the influence of ultrasonic activation (US) of the final irrigant in the removal of the photosensitizer from root canal walls after photodynamic therapy. METHODS:The root canals of 60 single-rooted bovine extracted teeth were filled with 0.01% methylene blue an...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.12.011
更新日期:2017-03-01 00:00:00
abstract:AIM:The aim of this study was to evaluate the effectiveness of final decontamination protocols against Enterococcus faecalis and their influence on bond strength of filling material to root canal dentin. MATERIAL AND METHODS:Ninety root canals were enlarged with ProTaper system and inoculated with E. faecalis for 15da...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.11.004
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:an accurate measurement of anterior chamber depth (ACD) both in eyes that underwent corneal refractive surgery (CRS) and in eyes that did not, can be crucial, as in case of intraocular lens (IOL) calculation in eyes that need to undergo cataract extraction, or during the evaluation of glaucomatous patients. ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101976
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:Quantitative Light-induced Fluorescence-Digital (QLF-D) detects red fluorescence of plaque, but is almost solely used on labial anterior tooth surfaces. As distribution and formation of plaque varies distinctly within the dentition, our aim was to investigate, how red fluorescing plaque is related to disclos...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.102063
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:Photodynamic therapy (PDT) is a well-known and effective treatment for non-melanoma skin-cancer. Numerous studies have also shown its effectiveness in mycosis fungoides. The aim of the study was to analyse MF patients treated with PDT at the Dermatology Unit of Bologna University. METHODS:We retrospectively...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2017.09.004
更新日期:2017-12-01 00:00:00
abstract:BACKGROUND:Long treatment periods to reach a substantial inactivation of microorganisms are one of the critical challenges in the photodynamic therapy field. METHODS:Planktonic suspensions of Streptococcus mutans were treated in different groups: presence of rose bengal (RB at 2μM) and light exposure by a new high pot...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.05.001
更新日期:2016-09-01 00:00:00
abstract:BACKGROUND:This study investigated the relationship between manual dexterity and dental biofilm accumulation in independent older Koreans using Quantitative Light-Induced Fluorescence-Digital (QLF-D). METHODS:This cross-sectional study included 44 participants recruited from senior welfare facilities in South Korea an...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,多中心研究
doi:10.1016/j.pdpdt.2018.11.007
更新日期:2019-03-01 00:00:00
abstract::Photophysical and in vitro photocytotoxicity studies were performed for the photosensitizer Dimegine, a disodium salt 2.4-di(alpha-methoxyethyl)-deuteroporphyrin-IX with very low systemic toxicity. The singlet oxygen and luminescence quantum yield were ΦΔ = 0,65 ± 0,06, and Φƒ=0,11 ± 0,01 respectively, and were indepe...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.11.006
更新日期:2019-03-01 00:00:00
abstract::Surgical resection for atypical polypoid adenomyoma (APA) is an option for fertility preservation. Due to the high recurrence rate of APA, studies have been conducted to improve total resection of tumors. Photodynamic diagnosis (PDD) using 5-aminolevulinic acid (5-ALA) improves tumor resection, but this has not been a...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.06.019
更新日期:2019-09-01 00:00:00
abstract::Hypericin photodynamic therapy (HypPDT) has been found to be an efficient inducer of cell death. However, there are indications that HypPDT also activates rescuing pathways. Cell responses to HypPDT are highly dependent on the Hyp intracellular localization and accumulation. We have shown previously that in U87 MG cel...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2014.02.010
更新日期:2014-06-01 00:00:00
abstract::Chronic renal failure (CRF) is a symptom of kidney damage in the terminal stages. If a patient is not treated, then CRF will progress to uremia, which greatly reduces the lifespan of the patient. However, current screening strategies, including routine urine tests and medical imaging investigations, have poor sensitiv...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101792
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Vascular-targeted photodynamic therapy (V-PDT) has shown good selectivity and efficacy in the treatment of certain types of vascular disease, including port wine stains, age-related macular degeneration, and esophageal varices. This study was conducted to test the efficacy and safety of V-PDT in the treatmen...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2011.11.003
更新日期:2012-06-01 00:00:00
abstract:PURPOSE:To investigate the autofluorescence (AF) characteristics of punctate inner choroidopathy (PIC) and to evaluate the progression rate of retinal pigment epithelium (RPE) atrophy lesions in PIC using confocal scanning laser ophthalmoscopy. METHODS:Twenty-two eyes of 14 PIC cases and 21 eyes of 21 non-proliferativ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2014.07.002
更新日期:2014-12-01 00:00:00
abstract:OBJECTIVE:The aim of this pilot study was to compare the efficacy of ALA-PDT and red light alone in the treatment of photoaging. METHODS:A total of 14 adults with photoaging skin were recruited. ALA-PDT or red light alone was applied to the forearm extensor. Before and after treatment, the treated sites were examined ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2014.02.007
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND:The treatment of periodontal disease focuses on eradication or suppression of the pathogenic microbiota within the periodontal pocket. There are some mechanical and chemical ways, and recently antimicrobial photodynamic therapy to eliminate the bacteria. The aim of this in vitro study was to compare the effe...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.04.003
更新日期:2016-09-01 00:00:00
abstract::The tumor microenvironment (TME) affects not only tumor growth and metastasis, but also therapy efficacy. In the present study, an oxygen self-supplying delivery system for a photosensitizer (O2-PDS), with an oxygen source of calcium peroxide (CPO), has been designed to induce multi-path tumor apoptosis through intera...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.101642
更新日期:2020-03-01 00:00:00