Abstract:
:Hypericin photodynamic therapy (HypPDT) has been found to be an efficient inducer of cell death. However, there are indications that HypPDT also activates rescuing pathways. Cell responses to HypPDT are highly dependent on the Hyp intracellular localization and accumulation. We have shown previously that in U87 MG cells Hyp localizes mostly in ER and partially in mitochondria, lysosomes and Golgi, and that HypPDT resulted primarily in apoptosis via the mitochondrial apoptotic pathway. We have also shown that Hyp co-localizes and interacts with anti-apoptotic PKCα in U87 MG cells. To follow up on our previous work, we investigated how HypPDT influences PKCα in U87 MG cells. Here, we show that majority of PKCα present in U87 MG cells is already in a catalytically competent form phosphorylated at Thr638, and it is a likely Bcl2 kinase. The presence of Hyp itself does not affect PKCα distribution. HypPDT acute effect caused PKCα activation and translocation along the plasma membrane and partially in the nuclei. The prolonged effect of HypPDT, 5 and 24h post PDT, results in PKCα located predominantly in cytosol and nuclei. Moreover, we have shown that phosphorylated catalytically competent PKCα is critical for U87 glioma cell viability in response to HypPDT treatment.
journal_name
Photodiagnosis Photodyn Therjournal_title
Photodiagnosis and photodynamic therapyauthors
Dzurová L,Petrovajova D,Nadova Z,Huntosova V,Miskovsky P,Stroffekova Kdoi
10.1016/j.pdpdt.2014.02.010subject
Has Abstractpub_date
2014-06-01 00:00:00pages
213-26issue
2eissn
1572-1000issn
1873-1597pii
S1572-1000(14)00031-3journal_volume
11pub_type
杂志文章abstract::Medication-related osteonecrosis of the jaw (MRONJ) results in progressive destruction of the jawbones, and advanced cases demand surgical intervention. The total removal of necrotic bone is required to prevent recurrence and infection. However, determining the limits between necrotic and healthy bone is a challenge. ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,评审
doi:10.1016/j.pdpdt.2020.102003
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVES:To evaluate the bleaching efficacy of a violet radiation (VR) combined or not with bleaching gels on the color and mineral content of stained teeth. MATERIAL AND METHODS:Enamel/dentin blocks were obtained and stained (n = 50) with coffee, red wine, tobacco smoke, or were left non-stained. The stained or not...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2021.102194
更新日期:2021-01-26 00:00:00
abstract:BACKGROUND:Microorganisms treated by photo-activated disinfection (PAD) in combined periodontal-endodontic (perio-endo) lesions would be exposed to sub-lethal doses of PAD (sPAD). This study evaluated the effect of sPAD using toluidine blue O (TBO) in combination with diode laser irradiation on the growth and biofilm-f...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.08.013
更新日期:2016-12-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to investigate the effects of 5-aminolaevulinic acid mediated photodynamic therapy (ALA-PDT) on the survival activity and apoptosis of human melanoma cell line A375 and non-melanoma skin carcinoma cell line A431 cells. The mechanism for cellular apoptosis was explored. METHODS:...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.01.004
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND:Recently, 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT) is being widely used in cancer therapy owing to the tumor-specific accumulation of photosensitizing protoporphyrin IX (PpIX) after the administration of ALA. In the present study, by focusing on genes involved in the porphyrin biosynthesis ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2011.12.004
更新日期:2012-09-01 00:00:00
abstract:BACKGROUND:Previous in vivo studies on photodynamic therapy (PDT)-treated, high cellular density tumors showed evidences of a bystander effect accompanying the therapy, cellular death continuing beyond the limits of the photochemical reactions in time and space. This process is generated by the initially damaged cells ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2012.03.001
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:The aim of this pilot study was to compare the efficacy of ALA-PDT and red light alone in the treatment of photoaging. METHODS:A total of 14 adults with photoaging skin were recruited. ALA-PDT or red light alone was applied to the forearm extensor. Before and after treatment, the treated sites were examined ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2014.02.007
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND:The potential application of TOOKAD(®)-PDT for the treatment of blood vessels was investigated. TOOKAD(®) (WST09), a novel palladium-bacteriopheophorbide absorbs light in the near IR with a high quantum yield of intersystem crossing. Our study assessed the efficacy of this drug in inducing vascular damage wi...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/S1572-1000(05)00006-2
更新日期:2005-03-01 00:00:00
abstract::Candida albicans biofilm is a main cause of infections associated with medical devices such as catheters, contact lens and artificial joint prosthesis. The current treatment comprises antifungal chemotherapy that presents low success rates. Photodynamic inactivation (PDI) involves the combination of a photosensitizing...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2016.12.003
更新日期:2017-03-01 00:00:00
abstract::The relationship between cytomegalovirus (CMV), a severe complication in immunocompromised patients, and graft-versus-host disease (GVHD) has already been reported but remains without complete elucidation, especially concerning oral lesions. Considering that antimicrobial photodynamic therapy (aPDT) has an antimicrobi...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101849
更新日期:2020-12-01 00:00:00
abstract:BACKGROUND:Photodynamic therapy (PDT) and diagnosis (PDD) using 5-aminolevulinic acid (ALA) to control the production of the intracellular photosensitizer protoporphyrin IX (PpIX) are commonly used clinically. Previously, we demonstrated that dormant and drug-induced dormancy-like cancer cells accumulated high PpIX lev...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101893
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:In this study, the photosensitivity of 5-aminolevulinic acid (5-ALA) conjugated gold nanoparticles (conjugate) has been investigated on the MeL-Rm cell line. METHODS:After culture and proliferation of the Mel-Rm cell line, cell toxicity and the suitable cell incubation time of the conjugate were determined....
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2013.03.010
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:Photodynamic therapy has apart from a direct cytotoxic effect also immunomodulatory properties. The aim of our study was to investigate how photodynamic therapy with 5-aminolevulinic acid (ALA-PDT) in sublethal doses influences the secretion of interleukins 6, 8 and 10 from colon cancer cells in vitro. METH...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.02.021
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVE:Evaluate the efficacy of 5-Aminolevulinic Acid Photodynamic Therapy (PDT) in medical treatment of nasal inverted papilloma (NIP). METHODS:Three patients with nasal inverted papilloma were treated with 5-Aminolevulinic Acid Photodynamic Therapy at our department from April to September 2012. The efficacy and ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2013.05.010
更新日期:2013-12-01 00:00:00
abstract::Follicular mucinosis (FM) is a rare inflammatory disorder histologically characterized by mucin deposition in the follicular epithelium. There is no standard therapy for FM and several treatments have been described in the literature. We present the case of a 59 year-old female affected by a recalcitrant FM with diffu...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.07.003
更新日期:2019-09-01 00:00:00
abstract::Photodynamic therapy (PDT) is a promising modality for the treatment of cancer. PDT involves administering a photosensitizing dye, i.e. photosensitizer, that selectively accumulates in tumors, and shining a light source on the lesion with a wavelength matching the absorption spectrum of the photosensitizer, that exert...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,评审
doi:10.1016/j.pdpdt.2019.04.033
更新日期:2019-06-01 00:00:00
abstract::We have more than 10 years experience in the Photobiology Unit, in the use of topical photodynamic therapy (PDT) for non-melanoma skin cancer and other skin diseases. During this time we have undertaken approximately 5000 treatments and this article details the practical aspects of how we treat Bowen's disease with to...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2009.04.001
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVES:The purpose of this study was to determine the usefulness of a quantitative light-induced fluorescence (QLF) technology in detecting dental restorations by comparing the detection ability of dental hygiene students between using conventional visual inspection alone and visual inspection combined with QLF tec...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.pdpdt.2016.12.010
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:Photodynamic therapy is a promising invention to treat infections and cancer where conventional treatments are insufficient and have many side effects. Photodynamic therapy is mainly emphasized as having minimal side effects on healthy cells during local applications, even so photosensitizer can accumulate i...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101891
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:We demonstrate a new diagnostic method, Photodiagnostic Infrared Spectroscoppy (PDIS), which is able to detect circulating tumor clusters and circulating tumor cells in the circulatory system. METHODS:The PDIS method is based on photodiagnostic physics and high resolution spectroscopy and is using calibrate...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101755
更新日期:2020-06-01 00:00:00
abstract::Surgical resection for atypical polypoid adenomyoma (APA) is an option for fertility preservation. Due to the high recurrence rate of APA, studies have been conducted to improve total resection of tumors. Photodynamic diagnosis (PDD) using 5-aminolevulinic acid (5-ALA) improves tumor resection, but this has not been a...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.06.019
更新日期:2019-09-01 00:00:00
abstract::Wounds constitute severe problems in public health. Inappropriate manipulation to promote wound healing and indiscriminate use of antibiotics may contribute to failure in wound treatment, leading to bacterial growth and resistance. Appropriate and correct approaches to wound treatment are crucially important. Further,...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101682
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Condyloma acuminatum is a highly infectious disease caused by the human papillomavirus. Previous studies have shown that 5-aminolevulinic acid photodynamic therapy can inhibit proliferation of condyloma acuminatum keratinocytes. However, the effect of 5-aminolevulinic acid photodynamic therapy on condyloma a...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.07.018
更新日期:2019-12-01 00:00:00
abstract::Metal-semiconductor core-shell type Pd@SiO2 nanoparticles (NPs) were successfully synthesized by Stober's method and the product was characterized by UV-vis, XRD, FT-IR, SEM, HR-TEM and EDX techniques. In vitro Photodynamic activity and DNA binding studies of Pd@SiO2 core shell nanoparticles were studied. Cell viabili...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.03.008
更新日期:2019-06-01 00:00:00
abstract:OBJECTIVE:To evaluate the clinical efficacy and safety of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) combined with a carbon dioxide laser (CO2 laser + PDT) versus CO2 laser for the treatment of low-grade vaginal intraepithelial neoplasms (vaginal LSIL). METHODS:We recruited 40 patients with vaginal LSIL and ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2020.101731
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Verrucous carcinoma of the penis is a rare disease characterized by verrucous lesions. The traditional treatment methods for verrucous carcinoma result in loss of sexual function are not satisfactory. The purpose of this study was to evaluate the safety and effect of topical aminolevulinic acid photodynamic ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.08.015
更新日期:2018-12-01 00:00:00
abstract:BACKGROUND/AIM:Laser-based photodynamic endoscopic diagnosis (LPDED) is a type of endoscopic diagnosis that uses the fluorescence caused by the photochemical reaction that occurs when a fluorescent substance is irradiated by a light of a specific wavelength. Although 5-aminolevulinic acid (5-ALA) can detect early gastr...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2018.02.005
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Extramammary Paget disease (EMPD) is an uncommon malignant neoplasm affecting apocrine gland-bearing skin which usually occurs in the anogenital area of patients older than 50 years. However, as EMPD in old men develops in penoscrotal area and sometimes asymptomatic, in situ or dermal invasion is usually wid...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2014.03.006
更新日期:2014-09-01 00:00:00
abstract::The objective of this study was to evaluate and compare the clinical response to PDT (Photodynamic Therapy) in field cancerization using two aminolevulinate derivatives. Forty patients with multiple actinic keratosis (AK) on forearms and hands scattered received two sessions of ALA and MAL-PDT at 630 nm (36 J/cm2). Th...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 杂志文章
doi:10.1016/j.pdpdt.2019.101603
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:The objective of this study was to improve the feasibility of applying topic 5-aminolevulinic acid (ALA) in photodiagnosis (PD) and treatment of condyloma caused by human papillomavirus (HPV) using two homemade handheld devices and to discuss the photodynamic therapy (PDT) as a suitable alternative for each ...
journal_title:Photodiagnosis and photodynamic therapy
pub_type: 临床试验,杂志文章
doi:10.1016/j.pdpdt.2011.09.001
更新日期:2012-03-01 00:00:00