Histological evaluation of cutaneous wound healing in presence of nitrous oxide in rats.

Abstract:

:Three-centimetre linear incisions were made in 90 young male Lew/f Mai rats by scalpel through skin to the investing fascia of the left flank. The skin was undermined sharply for a distance of 0.5 cm along both sides of the entire length of the incision. The wound site was closed per primam with Michel clips and left uncovered. Following emergence from anesthesia, rats were randomly allocated to one of three groups of 30 rats each: one group breathed air only and acted as control; the second was exposed to 20% N2O for 24 hours daily until sacrificed (continuous group); the third was exposed to 20% N2O for 8 hours daily until sacrificed (intermittent group). Ten rats from each of the three groups were sacrificed after 3, 7, or 10 days postincision. After sacrifice, sections from the wound stained with hematoxylin and eosin or reticulin were examined microscopically. No histological evidence of an effect on wound healing of skin occurred in rats exposed to 20% N2O.

journal_name

Anesth Analg

journal_title

Anesthesia and analgesia

authors

Shah NK,Kripke BJ,Sanzone CF,Cosman EB

doi

10.1213/00000539-197809000-00003

subject

Has Abstract

pub_date

1978-09-01 00:00:00

pages

527-33

issue

5

eissn

0003-2999

issn

1526-7598

journal_volume

57

pub_type

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