Abstract:
OBJECTIVE:Micro ribonucleic acids (miRNAs) and Hedgehog (Hh) signaling pathway play key roles in the proliferation, migration and invasion of tumor cells. The aim of this study was to investigate the role of miR-132 and Hh signaling pathway in the proliferation and apoptosis of pancreatic cancer cells, and to investigate the possible underlying mechanism. MATERIALS AND METHODS:The expressions of miR-132 and Shh (a ligand of Hh) in clinical pancreatic cancer specimens and pancreatic cancer cell lines were determined by qRT-PCR. Meanwhile, the correlation between the two molecules was analyzed. Pancreatic cancer cell line (MiaPaCe-2a) was transfected with miR-132 mimics and inhibitor. The effects of miR-132 up- and down-regulation on the expressions of miR-132, Shh, Cyclin-D1, cleaved Caspase-3 and cleaved Caspase-9 were detected. In addition, the exact role of miR-132 in the proliferation, apoptosis and distribution of MiaPaCe-2a cells were investigated. RESULTS:The expression level of miR-132 in pancreatic cancer specimens and pancreatic cancer cell lines was significantly elevated when compared with that of control group. Meanwhile, miR-132 expression was negatively correlated with the expression level of Shh. Moreover, transfection with miR-132 mimics evidently up-regulated miR-132 expression. Moreover, miR-132 up-regulation significantly decreased the mRNA and protein expressions of Shh, facilitated the proliferation of MiaPaCe-2a cells, reduced the protein expressions of Cyclin-D1, cleaved Caspase-3 and cleaved Caspase-9, and suppressed cell apoptosis. On the contrary, miR-132 inhibitor transfection significantly inhibited the proliferative activity of MiaPaCe-2a cells, decreased the proportion of cells in G1 phase, and increased the proportion of cells in G2/M phase. CONCLUSIONS:MiR-132 promotes proliferation and inhibits apoptosis of pancreatic cancer cells through Hh signaling pathway.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Zhao DW,Hou YS,Sun FB,Han B,Li SJdoi
10.26355/eurrev_201903_17236subject
Has Abstractpub_date
2019-03-01 00:00:00pages
1978-1985issue
5eissn
1128-3602issn
2284-0729journal_volume
23pub_type
杂志文章abstract:OBJECTIVE:Atrial fibrillation (AF) is the most common type of arrhythmia, especially in rheumatic heart disease (RHD) patients. The differences in structural remodeling and electrical remodeling between the left and right atrium associated with AF in RHD patients are well known, and alterations in the expression profil...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201810_16165
更新日期:2018-10-01 00:00:00
abstract:OBJECTIVE:Extracellular signal-regulated kinase (ERK)/mitogen activated protein kinase (MAPK) signal pathway participates in cell proliferation, cycle, and apoptosis. MiR-16 is down-regulated in the pituitary tumor. This study investigated the role and related mechanism of miR-16 on pituitary tumor proliferation, cycle...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201803_14464
更新日期:2018-03-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVE:DJ-1 is a negative regulator of PTEN and plays a role in tumorigenesis. Abnormal miR-203 expression is associated with pancreatic cancer. Bioinformatics analysis showed a targeted relationship between miR-203 and DJ-1 3'-UTR. This study investigated whether miR-203 regulates DJ-1 expression and its role in pa...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201910_19278
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:The diagnostic and staging approach for the mediastinal lymphadenopathies, with or whithout pulmonary lesions endoscopically visible, is based on transbronchial needle aspiration (TBNA) during fiberoptic bronchoscopy and on mediastinoscopy. One important factor impacting on TBNA sensitivity is the rapid on s...
journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in juvenile rats with nephrotic syndrome, and to explore its effects on inflammatory changes and renal injury. MATERIALS AND METHODS:24 Sprague-Dawley (SD) rats were randomly divi...
journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201905_17831
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doi:10.26355/eurrev_202002_20209
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journal_title:European review for medical and pharmacological sciences
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更新日期:2017-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2014-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2001-03-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2018-11-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2017-06-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2016-04-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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journal_title:European review for medical and pharmacological sciences
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更新日期:2018-09-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-05-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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doi:10.26355/eurrev_201809_15952
更新日期:2018-09-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201809_15809
更新日期:2018-09-01 00:00:00
abstract::Allergic bronchopulmonary aspergillosis (ABPA) occurs in 7-9% of patients with cystic fibrosis (CF) and causes a worsening of lung function and respiratory symptoms. Standard treatment of ABPA consists of oral steroids; however, higher corticosteroid therapy associated to antifungal agent (itraconazole) long-term trea...
journal_title:European review for medical and pharmacological sciences
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doi:
更新日期:2014-07-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2019-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-04-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
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更新日期:2020-10-01 00:00:00
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journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201712_13999
更新日期:2017-12-01 00:00:00