当前位置： SCI文献检索 > THROMBOSIS RESEARCH期刊下所有文献 > Factor XIII improves platelet adhesion to fibrinogen by protein disulfide isomerase-mediated activity.

Factor XIII improves platelet adhesion to fibrinogen by protein disulfide isomerase-mediated activity.

Abstract：

BACKGROUND:Factor XIII (FXIII), a plasma pro-transglutaminase, consists of two A subunits and two B subunits (FXIIIA2B2). Following activation by thrombin, it cross-links fibrin chains at the final step of coagulation. We previously reported that FXIII subunit A (FXIIIA) serves as a protein disulfide isomerase (PDI), and that PDI promotes platelet adhesion and aggregation. OBJECTIVE:This study sought to examine possible mechanistic effect of FXIII on platelet adhesion to fibrinogen; specifically, the role of its PDI activity. METHODS:Ex vivo experiments: Blood platelets derived from five patients with hereditary FXIIIA deficiency before and after treatment with Fibrogammin-P (FXIIIA2B2 concentrate) were washed and incubated on immobilized fibrinogen. Bound platelets were stained and counted by microscopy. In vitro experiments: Platelets derived from patients before treatment and five healthy controls were washed and analyzed for adhesion in the presence or absence of Fibrogammin-P or recombinant FXIII (FXIIIA2 concentrate). RESULTS:In ex vivo experiments, one hour after Fibrogammin-P treatment, mean (±SEM) platelet adhesion to fibrinogen increased by 27±2.32% (p<0.001). In in vitro experiments, treatment with Fibrogammin-P or recombinant FXIII (10IU/mL each) enhanced platelet adhesion to fibrinogen (in patients, by 29.95±6.7% and 29.05±5.3%, respectively; in controls, by 26.06±3.24% and 26.91±4.72, respectively; p<0.04 for all). Iodoacetamide-treated FXIII (I-FXIII), where transglutaminase activity is blocked, showed similar enhanced adhesion as untreated FXIII. By contrast, addition of an antibody that specifically blocks FXIIIA-PDI activity inhibited FXIII-mediated platelet adhesion to fibrinogen by 65%. CONCLUSION:These findings indicate that FXIII-induced enhancement of platelet adhesion is mediated by FXIII-PDI activity.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

Lahav J,Tvito A,Bagoly Z,Dardik R,Inbal A

doi

10.1016/j.thromres.2012.12.003

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

338-41

issue

4

eissn

0049-3848

issn

1879-2472

pii

S0049-3848(12)00858-4

journal_volume

131

pub_type

杂志文章

相关文献

THROMBOSIS RESEARCH文献大全
  • The direct binding of human factor VII in plasma to recombinant human tissue factor.

    abstract：:The extrinsic pathway of coagulation is initiated when zymogen factor VII binds to its cell surface receptor tissue factor. Recently recombinant human tissue factor has become available and therefore in this study the direct binding of human factor VII in plasma to recombinant tissue factor was explored. Factor VII bi...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(93)90103-u

    authors： Sridhara S,Clarke BJ,Ofosu FA,High KA,Blajchman MA

    更新日期：1993-05-15 00:00:00

  • Accumulation of platelets as a key mechanism of human erection.

    abstract：:In this study, the role of platelets in the human erectile response was assessed. Twenty patients with erectile dysfunction were studied by means of (111)In-oxine-platelets and blood pool imaging using 99mTc. Seventeen patients received intracavernously prostaglandin E1, and three patients received papaverine together...

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0049-3848(98)00065-6

    authors： Rodrigues M,O'Grady J,Stackl W,Granegger S,Sinzinger H

    更新日期：1998-07-15 00:00:00

  • Thrombin generation post elective caesarean section: effect of low molecular weight heparin.

    abstract：INTRODUCTION:Caesarean section (CS) is a significant risk factor for venous thromboembolism.. Low molecular weight heparin (LMWH) is commonly used for thromboprophylaxis post emergency caesarean delivery. However, no consensus exists regarding LMWH thromboprophylaxis following elective caesarean section. Measures of th...

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.thromres.2012.01.008

    authors： Ismail SK,Norris L,Muttukrishna S,Higgins JR

    更新日期：2012-11-01 00:00:00

  • The use of eConsults to improve access to specialty care in thrombosis medicine.

    abstract：INTRODUCTION:Electronic consultations (eConsults) are asynchronous, consultative electronic-based communications that are aimed to improve efficient and timely access to specialist advice. Our study aim was to evaluate the use and impact of the Canadian Champlain BASE™ (Building Access to Specialists through eConsultat...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2017.11.002

    authors： Skeith L,Mohamed M,Karovitch A,Liddy C,Afkham A,Archibald D,Keely E

    更新日期：2017-12-01 00:00:00

  • The value of tomographic ventilation/perfusion scintigraphy (V/PSPECT) for follow-up and prediction of recurrence in pulmonary embolism.

    abstract：BACKGROUND:Pulmonary embolism (PE) is diagnosed with imaging techniques such as ventilation/perfusion (V/P) lung scintigraphy or multidetector computed tomography of the pulmonary arteries (MDCT). Lung scintigraphy can be performed with planar (V/P PLANAR) and tomographic (V/P SPECT) techniques. V/P SPECT has higher se...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2012.09.002

    authors： Alhadad A,Miniati M,Alhadad H,Gottsäter A,Bajc M

    更新日期：2012-12-01 00:00:00

  • Cilostazol inhibits HMGB1 release in LPS-activated RAW 264.7 cells and increases the survival of septic mice.

    abstract：INTRODUCTION:Inflammation and coagulation play important roles in the pathogenesis of sepsis. Anticoagulants with anti-inflammatory action draw attention as therapeutic agent in sepsis. OBJECTIVE:Whether cilostazol (6-[4-(1-cyclohexyl-1H-tetrazol-5-yl) butoxy]-3,4-dihydro-2-(1H)-quinolinone), anticoagulant, protects m...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2015.06.017

    authors： Chang KC

    更新日期：2015-08-01 00:00:00

  • Correlation between plasma levels of growth factors and von Willebrand factor.

    abstract：:Intimal proliferation of smooth muscle cells is an important characteristic of developing atherosclerotic lesions and late occlusion of venous bypass grafts. Platelet-derived growth factor, released from aggregating platelets at sites of endothelial injury, has been suggested as a main factor responsible for intimal h...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(89)90042-x

    authors： Nilsson J,Elgue G,Wallin M,Hamsten A,Blombck M

    更新日期：1989-04-15 00:00:00

  • Screening itself for disseminated intravascular coagulation may reduce mortality in sepsis: A nationwide multicenter registry in Japan.

    abstract：OBJECTIVES:Screening of patients with sepsis for disseminated intravascular coagulation (DIC) has been recommended in several guidelines. However, DIC screening is still not widely accepted as an essential component of sepsis management, partly because of a lack of evidence that DIC screening has an effect on mortality...

    journal_title：Thrombosis research

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.thromres.2017.11.023

    authors： Umemura Y,Yamakawa K,Hayakawa M,Hamasaki T,Fujimi S,Japan Septic Disseminated Intravascular Coagulation (J-Septic DIC) study group.

    更新日期：2018-01-01 00:00:00

  • Treatment of haemophilia patients in East Germany prior to and after reunification in 1990.

    abstract：:The reunification of Germany in 1990 brought with it major challenges in terms of unifying the care offered to haemophilia patients. At that time, most of the treatment centres belonged to the largest regional hospitals. The centre for paediatric haemophilia patients in Leipzig was at the University Hospital. In this ...

    journal_title：Thrombosis research

    pub_type： 历史文章,杂志文章,评审

    doi：10.1016/j.thromres.2013.10.018

    authors： Lenk H

    更新日期：2014-11-01 00:00:00

  • Platelet aggregation by a phospholipase C from Pseudomonas aeruginosa.

    abstract：:The effects and mechanism of action of a phospholipase C (PLC) from Pseudomonas aeruginosa on human platelet rich plasma were examined to better understand the interaction of PLC with human platelets. PLC caused platelet aggregation in a concentration-dependent manner. Enzymatic activity of PLC was necessary for aggre...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(88)90115-6

    authors： Coutinho IR,Berk RS,Mammen E

    更新日期：1988-09-01 00:00:00

  • Synergistic potentiation by epinephrine of collagen or thrombin-induced calcium mobilization in human platelets.

    abstract：:Changes in cytoplasmic free calcium in response to epinephrine in combination with other agonists were studied in human platelets loaded with the fluorescent calcium indicator, quin 2. In the presence of 1mM external calcium, epinephrine augmented the increase in cytoplasmic free calcium in response to collagen or thr...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(87)90153-8

    authors： Ardlie NG,Bell LK,McGuiness JA

    更新日期：1987-05-15 00:00:00

  • Comparison of platelet aggregability and P-selectin surface expression on platelets isolated by different methods.

    abstract：:Three methods commonly used for isolation of blood platelets from plasma were compared. Platelets were isolated by: 1) a washing method; 2) a method of metrizamide-gradient centrifugation; 3) a modified method of gel-filtration. The last method employed BSA-Sepharose gel instead of routinely used Sepharose gel saturat...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(00)00282-6

    authors： Walkowiak B,Kralisz U,Michalec L,Majewska E,Koziolkiewicz W,Ligocka A,Cierniewski CS

    更新日期：2000-09-01 00:00:00

  • Construction and its expression of a new retroviral vector containing a human blood coagulation factor IX cDNA.

    abstract：:We constructed a new factor IX expression vector containing a full length factor IX cDNA. The Moloney Murine Leukemia Virus (MoMLV)-based retroviral vector pLRNL was cloned with the entire coding region of human factor IX down stream of the promoter of Rous sarcoma virus, giving rise to the construct pL9RNL. The GP+E ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(93)90038-p

    authors： Matsushita T,Emi N,Takahashi I,Takamatsu J,Saito H

    更新日期：1993-02-15 00:00:00

  • Comparison of microvesicle tissue factor activity in non-cancer severely ill patients and cancer patients.

    abstract：INTRODUCTION:Increased levels of circulating tissue factor (TF)-positive microvesicles (MVs) may increase the risk of thrombosis. Indeed, TF-positive MVs are detected in plasma of patients with various types of diseases. In this study, we measured levels of MV TF activity in non-cancer severely ill patients and cancer ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2018.03.001

    authors： Hisada Y,Thålin C,Lundström S,Wallén H,Mackman N

    更新日期：2018-05-01 00:00:00

  • Studies on the anticoagulant action of Aspilia africana.

    abstract：:An anticoagulant activity was identified and isolated from the leaves of a West African plant, Aspilia africana by gel filtration on Sephadex G-100. The anticoagulant factor had an apparent molecular weight of approximately 60,000 d. Upon incubation with plasma, it prolonged the partial thromboplastin time, prothrombi...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(87)90455-5

    authors： Hanna MM,Niemetz J

    更新日期：1987-08-15 00:00:00

  • Intravascular cysteinyl-leukotriene formation by clotting whole human blood. Evidence from clamped umbilical vein segments and thrombus specimens.

    abstract：:We have recently demonstrated that contact activation of the intrinsic coagulation cascade in vitro is accompanied not only by thromboxane (TX) B2 generation but also by the formation of 5-lipoxygenase-derived cysteinyl-leukotrienes (LT). In our present study we have investigated the effects of the vascular wall on th...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(93)90175-n

    authors： Weide I,Winking M,Simmet T

    更新日期：1993-10-01 00:00:00

  • Abnormally high prevalence of major components of the metabolic syndrome in subjects with early-onset idiopathic venous thromboembolism.

    abstract：BACKGROUND:Although patients with idiopathic VTE are at higher than normal risk of asymptomatic atherosclerosis and of cardiovascular events, the impact of cardiovascular risk factors on VTE is poorly understood. OBJECTIVE:To assess the prevalence of the metabolic syndrome and of its components in patients with early-...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2010.12.005

    authors： Di Minno MN,Tufano A,Guida A,Di Capua M,De Gregorio AM,Cerbone AM,Tarantino G,Di Minno G

    更新日期：2011-03-01 00:00:00

  • Circulating platelet-leukocyte aggregates: a marker of microvascular injury in diabetic patients.

    abstract：:Diabetes is associated with multiple disorders including metabolic, cellular and blood disturbances leading to vascular complications. Increased circulating levels of platelet-leukocyte aggregates (PLA) have been described in several thrombotic diseases. In this study, we have evaluated circulating PLA in diabetic pat...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2007.07.016

    authors： Elalamy I,Chakroun T,Gerotziafas GT,Petropoulou A,Robert F,Karroum A,Elgrably F,Samama MM,Hatmi M

    更新日期：2008-01-01 00:00:00

  • Fluorescent activity-based probe for the selective detection of Factor VII activating protease (FSAP) in human plasma.

    abstract：:The zymogen form of circulating Factor VII activating protease (FSAP) is activated by histones that are released as a consequence of tissue damage or excessive inflammation. This is likely to have consequences in a number of disease conditions such as stroke, atherosclerosis, liver fibrosis, thrombosis and cancer. To ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2019.08.016

    authors： Rut W,Nielsen NV,Czarna J,Poreba M,Kanse SM,Drag M

    更新日期：2019-10-01 00:00:00

  • Postpartum blood transfusion and hemorrhage as independent risk factors for venous thromboembolism.

    abstract：INTRODUCTION:Profuse postpartum hemorrhage (PPH) and red blood cell (RBC) transfusion have been suggested to be associated with venous thromboembolic events (VTE). However, it is not fully clear if they are independent major risk factors. METHODS:Women who gave birth in the Stockholm area between 1999 and 2002 were th...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2018.03.002

    authors： Thurn L,Wikman A,Lindqvist PG

    更新日期：2018-05-01 00:00:00

  • Acquired activated protein C resistance in pregnancy.

    abstract：:Pregnancy induces several haemostatic perturbations. Some authors described possible acquired activated protein C resistance (APCR) during normal pregnancy. We wanted to test this possibility and to evaluate if this acquired APCR might contribute to the known increased tendency to thrombosis associated with pregnancy....

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章

    doi：10.1016/0049-3848(96)00177-6

    authors： Schlit AF,Col-De Beys C,Moriau M,Lavenne-Pardonge E

    更新日期：1996-11-01 00:00:00

  • Subcutaneous treatment of deep venous thrombosis with low molecular weight heparin. A dose finding study with LMWH-Novo.

    abstract：:Treatment of deep venous thrombosis with low molecular weight heparin (LMWH-Novo, Logiparin) was carried out with two different doses of Logiparin, 75 XaI U/kg b.w. twice daily and 150 XaI U/kg b.w. once daily subcutaneously for 5 days. Simultaneously warfarin was given from the first day of heparin treatment. Mean ag...

    journal_title：Thrombosis research

    pub_type： 临床试验,杂志文章

    doi：10.1016/0049-3848(89)90307-1

    authors： Siegbahn A,Y-Hassan S,Boberg J,Bylund H,Neerstrand HS,Ostergaard P,Hedner U

    更新日期：1989-09-15 00:00:00

  • Factors that affect the size of platelet aggregates in epinephrine-induced activation: a study using the particle counting method based upon light scattering.

    abstract：:Platelet aggregate size was determined with a newly-developed platelet aggregometer, PA-100 (KOWA), which can quantitatively evaluate the size and number of platelet aggregates by means of the particle counting method based upon light scattering. Epinephrine-induced platelet aggregation consists of two phases, the for...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(96)00026-6

    authors： Satoh K,Ozaki Y,Qi R,Yang L,Asazuma N,Yatomi Y,Kume S

    更新日期：1996-03-01 00:00:00

  • Antithrombotic activity of a synthetic heparin pentasaccharide in a rabbit stasis thrombosis model using different thrombogenic challenges.

    abstract：:A synthetic pentasaccharide, representing the critical sequence required in heparin for binding to antithrombin III (AT III), produces strong anti-factor Xa activity in vitro in the presence of AT III and is devoid of any activity directed towards thrombin. This pentasaccharide provides a unique tool to study the ques...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/0049-3848(87)90280-5

    authors： Walenga JM,Petitou M,Lormeau JC,Samama M,Fareed J,Choay J

    更新日期：1987-04-15 00:00:00

  • CCL5 deficiency reduces neointima formation following arterial injury and thrombosis in apolipoprotein E-deficient mice.

    abstract：:Activated platelets secrete different chemokines, among others CCL5, thereby triggering inflammatory cell recruitment into the vessel wall. Here, we investigated how CCL5 deficiency influences vascular remodeling processes. Experiments were performed in apolipoprotein E and CCL5 double deficient (ApoE(-/-)×CCL5(-/-)) ...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2016.06.013

    authors： Czepluch FS,Meier J,Binder C,Hasenfuss G,Schäfer K

    更新日期：2016-08-01 00:00:00

  • Tinzaparin in cancer associated thrombosis beyond 6months: TiCAT study.

    abstract：INTRODUCTION:The safety and efficacy of low-molecular-weight heparin (LMWH) treatment in patients with cancer-associated thrombosis (CAT) beyond 6months are unknown. Our aim was to determine the safety of long-term tinzaparin use in patients with CAT. METHODS:We performed a prospective, open, single arm, multicentre s...

    journal_title：Thrombosis research

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.thromres.2017.07.004

    authors： Jara-Palomares L,Solier-Lopez A,Elias-Hernandez T,Asensio-Cruz M,Blasco-Esquivias I,Marin-Barrera L,de la Borbolla-Artacho MR,Praena-Fernandez JM,Montero-Romero E,Navarro-Herrero S,Serrano-Gotarredona MP,Sánchez-Díaz JM,Palacios

    更新日期：2017-09-01 00:00:00

  • Conditional knockout of tissue factor pathway inhibitor 2 in vascular endothelial cells accelerates atherosclerotic plaque development in mice.

    abstract：BACKGROUND:Tissue factor pathway inhibitor-2 (TFPI-2) regulates matrix metalloproteinases activation and extracellular matrix degradation. Over-expression of TFPI-2 enhances atherosclerotic plaque stability. The aim of this study is to investigate the effect of conditional knockout (KO) of TFPI-2 in vascular endothelia...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2015.11.010

    authors： Hong J,Liu R,Chen L,Wu B,Yu J,Gao W,Pan J,Luo X,Shi H

    更新日期：2016-01-01 00:00:00

  • VTE prophylaxis for the medical patient: where do we stand? - a focus on cancer patients.

    abstract：:Acutely ill medical patients are at moderate to high risk of venous thromboembolism (VTE): approximately 10-30% of general medical patients may develop deep-vein thrombosis or pulmonary embolism, and the latter is a leading contributor to deaths in hospital. Medical conditions associated with a high risk of VTE includ...

    journal_title：Thrombosis research

    pub_type： 杂志文章,评审

    doi：10.1016/S0049-3848(10)70008-6

    authors： Cohen AT,Nandini B,Wills JO,Ota S

    更新日期：2010-04-01 00:00:00

  • Plasminogen activator inhibitor-1 4G/5G genotype and residual venous occlusion following acute unprovoked deep vein thrombosis of the lower limb: A prospective cohort study.

    abstract：BACKGROUND:A recent study suggested that the plasminogen activator inhibitor (PAI)-1 4G/5G genotype may play a role in the resolution of deep vein thrombosis (DVT) after surgery. In the present study, we investigated the association between PAI-1 4G/5G genotype and the persistence of venous occlusion after acute idiopa...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/j.thromres.2017.03.017

    authors： Giurgea GA,Brunner-Ziegler S,Jilma B,Sunder-Plassmann R,Koppensteiner R,Gremmel T

    更新日期：2017-05-01 00:00:00

  • Superiority of enoxaparin over heparin in combination with a GPIIb/IIIa receptor antagonist during coronary thrombolysis in dogs.

    abstract：:It is known that a low-molecular-weight heparin (LMWH) is more effective than unfractionated heparin in unstable angina/non-Q-wave myocardial infarction (UA/NQMI) and the platelet GPIIb/IIIa receptors play an important role in acute myocardial infarction (AMI). Therefore, enoxaparin might have a similar advantage over...

    journal_title：Thrombosis research

    pub_type： 杂志文章

    doi：10.1016/s0049-3848(01)00242-0

    authors： Rebello SS,Kasiewski CJ,Bentley RG,Morgan SR,Chu V,Bostwick JS,Klein SI,Perrone MH,Leadley RJ

    更新日期：2001-05-01 00:00:00