Calcium paradox in skeletal muscles: physiologic and microscopic observations.

Abstract:

:Immersion of rat hemidiaphragms in Ca2+-free Krebs solution (KS) containing Ca2+ chelator in vitro leads to separation of basal lamina from the plasma membrane, as well as transient contracture and rapid loss of twitch response [calcium paradox (CP) phase 1]. Subsequent immersion in regular KS results in necrosis of muscle fibers accompanied by slowly increasing contracture (CP phase 2). This contracture could be prevented or reduced by using either Ca2+-free KS or calcium channel blockers, but not by dantrolene sodium, implying that after drastic reduction of extracellular and sarcolemmal Ca2+ during CP phase 1, the sarcolemma has lost its ability to control normal Ca2+ fluxes. Contracture did not develop at 21 degrees C. CP is a convenient model to study calcium-induced muscle cell death and the role of Ca2+ in maintaining sarcolemmal integrity.

journal_name

Muscle Nerve

journal_title

Muscle & nerve

authors

Soza M,Karpati G,Carpenter S

doi

10.1002/mus.880090306

subject

Has Abstract

pub_date

1986-03-01 00:00:00

pages

222-32

issue

3

eissn

0148-639X

issn

1097-4598

journal_volume

9

pub_type

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