Abstract:
OBJECTIVE:The purpose of this study was to explore the mechanism of micro-ribonucleic acid (miR)-214-3p in regulating fracture healing in rats with osteoporosis. MATERIALS AND METHODS:A total of 30 female Sprague-Dawley rats were selected and randomly divided into 3 groups, including group A [phosphate-buffered saline (PBS), n=10], group B (AntagomiR-NC, n=10), and group C (AntagomiR-214-3p, n=10). All rats underwent ovariectomy, and the osteoporosis rat model was verified by dual-energy X-ray absorptiometry 8 weeks after the operation. Then the osteoporotic fracture was established in rats via a second operation. From the successful modeling until the 6th week, 50 μL PBS (2 nmoL) was intraperitoneally injected in group A, an equal amount of AntagomiR-NC was injected in group B, and an equal amount of AntagomiR-214-3p was injected in group C once a week. At the 6th week, fracture healing of osteoporosis rats was evaluated. At the same time, the expression of miR-214-3p in the three groups was detected via reverse Transcription-Polymerase Chain Reaction (RT-PCR). Furthermore, the protein expressions of bone morphogenetic protein 2 (BMP2) and Smad4 in the three groups were detected via Western blotting (WB). RESULTS:After ovariectomy, the bone mineral density in each group was significantly lower than that before ovariectomy, and the differences were statistically significant (p<0.05). Imaging evaluation demonstrated that compared with group A and B, there were significantly more callus tissues in group C. Meanwhile, the fracture line healing was better and blurred, and the internal fixation had no displacement and loosening. RT-PCR results indicated that the expression level of miR-214-3p in group C was significantly lower than that of the other two groups (p<0.05). WB results showed that the protein expression levels of BMP2 and Smad4 in group C were significantly higher than those of group A and group B (p<0.05). CONCLUSIONS:MiR-214-3p delays fracture healing in rats with osteoporotic fracture by inhibiting the BMP/Smad signaling pathway.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Zhou LG,Shi P,Sun YJ,Liu HZ,Ni JQ,Wang Xdoi
10.26355/eurrev_201901_16854subject
Has Abstractpub_date
2019-01-01 00:00:00pages
449-455issue
2eissn
1128-3602issn
2284-0729journal_volume
23pub_type
杂志文章abstract:OBJECTIVE:The aim of this study was to investigate the influences of propofol on myocardial ischemia/reperfusion injury in rats through the Janus kinase/signal transducers and the activators of transcription (JAK/STAT) signaling pathway. MATERIALS AND METHODS:A total of 48 Sprague-Dawley (SD) rats were randomly divide...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201907_18456
更新日期:2019-07-01 00:00:00
abstract::Periprosthetic Joint Infection (PJI) represents one of the leading causes of revision prosthetic surgery, accounting for 25% of failed Total Knee Replacement (TKR) and 15% of failed Total Hip Replacement (THR). The search for a biomarker that, together with clinical and radiological findings, could improve the managem...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:10.26355/eurrev_201904_17473
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVE:Pharmacological post-conditioning (PC) by intermittent but not continuous administration of exogenous bradykinin (BK) reduces ischemia/reperfusion (I/R) injury via the Reperfusion Injury Salvage Kinase (RISK) pathway activation. We evaluated whether intermittent administration with icatibant (HOE140), a BK2R ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201905_17953
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the expression level of microRNA-21-5p and its target gene PLAG1 in the serum of patients with spinal cord injury (SCI), as well as the role and mechanism of microRNA-21-5p in lipopolysaccharide-induced primary microglial inflammation. PATIENTS AND METHODS:The levels ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202006_21480
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:Frontal recess is the anatomical region most difficult to manage in endoscopic frontal sinus surgery due to the extreme variability of the cell patterns that may be observed in this area. CT has always been the gold standard in preoperative evaluation, but especially in the assessment of the causes of fronta...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-07-01 00:00:00
abstract:OBJECTIVE:Acrodermatitis Continua of Hallopeau (ACH) is a variant of pustular psoriasis often very difficult to treat. Secondary syndactyly, also called "pseudosyndactyly", is rare and can be a complication of burns, dystrophic epidermolysis bullosa or trauma. If left untreated, joint complications and definitive funct...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2015-08-01 00:00:00
abstract:OBJECTIVE:To explore the correlations between the degree of coronary artery stenosis with blood lipid, C-reactive protein (CRP), homocysteine (Hcy), gamma-glutamyl transpeptidase (GGT), soluble cluster determinant 36 (sCD36), and fibrinogen (Fib) levels in elderly patients with coronary heart disease. PATIENTS AND MET...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201911_19453
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVE:LncRNAs participate in the proliferation, apoptosis, and invasion of colorectal cancer. We aimed at investigating the uncovered effect of lncRNALUADT1 on colorectal cancer. PATIENTS AND METHODS:The relative expression level of lncRNALUADT1 in tumor specimen was tested by Real-time quantitative PCR. The assoc...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201804_14816
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVE:Long noncoding RNA (lncRNA) GIHCG has been reported as an oncogene in hepatocellular carcinoma. However, the expression, roles, and clinical values of GIHCG in renal cell carcinoma (RCC) remain unclear. The aim of this study was to investigate the expression, roles, diagnostic and prognostic values of GIHCG i...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201801_14099
更新日期:2018-01-01 00:00:00
abstract:OBJECTIVE:To screen the differentially expressed circular ribonucleic acids (circRNAs) related to gastric cancer and to explore their associations with the clinicopathological features of gastric cancer. PATIENTS AND METHODS:Cancer tissues of 50 gastric cancer patients undergoing surgical resection in our hospital fro...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202003_20667
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:Nickel allergy is the most frequent contact allergy in the industrialized country. In allergic contact dermatitis after the presentation of haptenated peptides by resident or newly recruited skin cells, activated CD8+ T cells release IFN-γ and TNF-α, these cytokines are potent activator of keratinocytes. The ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:This study aimed to explore the role of miR-155-5p in middle cerebral artery occlusion/reperfusion (MCAO/R) model in rats and oxygen-glucose deprivation/reoxygenation (OGD/R)-induced SH-SY5Y cells. In addition, this study also aimed to explore the underlying mechanisms to expect that miR-155-5p may be investi...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202002_20198
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:To identify the potential role of miR-490-3p in the development of esophageal squamous cell carcinoma (ESCC), and to explore the possible underlying mechanism. PATIENTS AND METHODS:Human ESCC tissues and cancer-adjacent normal tissues were collected. The mRNA expression level of miR-490-3p was detected by qu...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201812_16527
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:CircRNAs serve an essential role in regulating the development and progression of various tumors. The aim of this study was to examine the role and mechanism of circ_0009910 in hepatocellular carcinoma (HCC). MATERIALS AND METHODS:RT-qPCR was used to detect the expression of circ_0009910 and miR-335-5p in ti...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202002_20349
更新日期:2020-02-01 00:00:00
abstract:OBJECTIVE:Previous studies have found that IPO5 is a cancer-promoting gene. However, the role of IPO5 in esophageal cancer has not been reported. This study aims to investigate the expression characteristics of IPO5 in esophageal cancer, and to further analyze its relationship with clinical parameters and prognosis of ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202004_21004
更新日期:2020-04-01 00:00:00
abstract:AIM:The efficacy of dietary inclusion of various parts of mangosteen (Garcinia mangostana L.) extract on growth and hematological parameters of African catfish (Clarias gariepinus) fingerlings were investigated. MATERIAL AND METHODS:Ninety six fingerlings were randomly distributed into each of ten 20L plastic aquaria....
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to explore the effects of cerebral ischemic preconditioning which can decrease brain ischemia/reperfusion (I/R) injury by inhibiting endoplasmic reticulum (ER) stress-induced apoptosis. MATERIALS AND METHODS:The focal cerebral ischemia rat was selected as the experimental model....
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201712_14020
更新日期:2017-12-01 00:00:00
abstract::Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "MiR-221 affects proliferation and apoptosis of gastric cancer cells through targeting SOCS3, by Q.-Y. Zhou, P.-L. Peng, Y.-H. Xu, published in Eur Rev Med...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202007_22226
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:The previous research revealed that long noncoding RNAs (lncRNAs) play a vital role in the development of hepatocellular carcinoma (HCC). To further discuss the underlying mechanisms of lncRNA DCST1-AS1 in the pathogenesis of HCC. PATIENTS AND METHODS:We screened the abnormally expressed genes in HCC tissues...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201907_18423
更新日期:2019-07-01 00:00:00
abstract:OBJECTIVE:Eribulin mesylate (Halaven®) is a non-taxane inhibitor of microtubule indicated as monotherapy in patients with metastatic breast cancer (MBC), which progresses after anthracycline and taxanes therapy. In this retrospective observational study, we want to evaluate the efficacy of Eribulin in elderly women wit...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202012_24171
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:To detect the expression level of long intergenic non-protein coding RNA 1198 (LINC01198) in colorectal cancer (CRC) tissues and cells, to investigate the effect of LINC01198 on the biological function of CRC cells through in vivo and in vitro experiments, and to explore its molecular mechanism. PATIENTS AND...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202008_22641
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:The diagnosis of spondylodiscitis can be difficult, because the patients history, subjective symptoms and physical findings are often inconclusive, particularly in the early stages. AIM:To perform an overview on the role of nuclear medicine procedures with single photon emission tomography (SPET) and positr...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2012-04-01 00:00:00
abstract::The authors evaluated the efficacy of an electronic treatment for pain, using an ion flow generator, BE-101 model by Bio-Ejt, on 19 patients suffering from acute pain of an arthrorheumatic nature. Each patient was treated for two weeks every other day (6 sittings), each sitting lasting 20 minutes at an intensity of ab...
journal_title:European review for medical and pharmacological sciences
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-03-01 00:00:00
abstract:OBJECTIVE:To study the expression level of the long non-coding ribonucleic acid (lncRNA) KCNQ1 overlapping transcript 1 (KCNQ1OT1) in the patients with myelodysplastic syndrome (MDS), as well as the correlation between the expression level of lncRNA KCNQ1OT1 and the clinical diagnosis and prognosis of MDS. PATIENTS AN...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202005_21341
更新日期:2020-05-01 00:00:00
abstract:OBJECTIVES:CHARGE is an acronym referring to the aspects of this rare syndromic condition. Patients with CHARGE association are today considered as subjects lacking in pathognomonic dental alterations. The present study is aimed at adding to the body of evidence of the cases reported in literature and the continuous cl...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:Age-related macular degeneration (AMD) is mainly characterized by dysfunction of retinal pigment epithelium (RPE) cells. This study aimed to investigate the protective effects of resveratrol on oxidative damaged RPE cells. MATERIALS AND METHODS:Human D407 cells were divided into normal control (NC), H2O2 tre...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201901_16786
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:Diabetes mellitus (DM) causes organ dysfunction and increases the sensitivity of organs to damages.To test this hypothesis, we used renal ischemia/reperfusion (I/R) experiment to evaluate the renoprotective activity of telmisartan versus pioglitazone on I/R induced renal damage in diabetic rats. MATERIALS A...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-05-01 00:00:00
abstract:OBJECTIVE:This paper aims at screening the common differential genes of coronary atherosclerotic heart disease (CAD) and ischemic cardiomyopathy (ICM), and to conduct pathway analysis and protein-protein interaction (PPI) network analysis for the differential genes. MATERIALS AND METHODS:The CAD and ICM datasets were ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201806_15182
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVE:To explore whether Ipriflavone could prevent postmenopausal osteoporosis (PMOP) and improve bone quality via promoting osteogenesis of bone marrow-derived mesenchymal stem cell (MSCs). MATERIALS AND METHODS:MSCs were extracted from rats and identified using flow cytometry. Osteogenic specific genes and adipo...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201807_15527
更新日期:2018-07-01 00:00:00
abstract:OBJECTIVE:Patients with severe burns often develop acute lung injury (ALI), systemic inflammatory response syndrome (SIRS) often complicates with ALI. Sivelestat sodium hydrate is an effective drug against ALI. However, the mechanisms of this beneficial effect are still poorly understood. In the current study, we evalu...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00