MiR-214-3p delays fracture healing in rats with osteoporotic fracture through inhibiting BMP/Smad signaling pathway.

Abstract:

OBJECTIVE:The purpose of this study was to explore the mechanism of micro-ribonucleic acid (miR)-214-3p in regulating fracture healing in rats with osteoporosis. MATERIALS AND METHODS:A total of 30 female Sprague-Dawley rats were selected and randomly divided into 3 groups, including group A [phosphate-buffered saline (PBS), n=10], group B (AntagomiR-NC, n=10), and group C (AntagomiR-214-3p, n=10). All rats underwent ovariectomy, and the osteoporosis rat model was verified by dual-energy X-ray absorptiometry 8 weeks after the operation. Then the osteoporotic fracture was established in rats via a second operation. From the successful modeling until the 6th week, 50 μL PBS (2 nmoL) was intraperitoneally injected in group A, an equal amount of AntagomiR-NC was injected in group B, and an equal amount of AntagomiR-214-3p was injected in group C once a week. At the 6th week, fracture healing of osteoporosis rats was evaluated. At the same time, the expression of miR-214-3p in the three groups was detected via reverse Transcription-Polymerase Chain Reaction (RT-PCR). Furthermore, the protein expressions of bone morphogenetic protein 2 (BMP2) and Smad4 in the three groups were detected via Western blotting (WB). RESULTS:After ovariectomy, the bone mineral density in each group was significantly lower than that before ovariectomy, and the differences were statistically significant (p<0.05). Imaging evaluation demonstrated that compared with group A and B, there were significantly more callus tissues in group C. Meanwhile, the fracture line healing was better and blurred, and the internal fixation had no displacement and loosening. RT-PCR results indicated that the expression level of miR-214-3p in group C was significantly lower than that of the other two groups (p<0.05). WB results showed that the protein expression levels of BMP2 and Smad4 in group C were significantly higher than those of group A and group B (p<0.05). CONCLUSIONS:MiR-214-3p delays fracture healing in rats with osteoporotic fracture by inhibiting the BMP/Smad signaling pathway.

authors

Zhou LG,Shi P,Sun YJ,Liu HZ,Ni JQ,Wang X

doi

10.26355/eurrev_201901_16854

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

449-455

issue

2

eissn

1128-3602

issn

2284-0729

journal_volume

23

pub_type

杂志文章
  • Effect of propofol on myocardial ischemia/reperfusion injury in rats through JAK/STAT signaling pathway.

    abstract:OBJECTIVE:The aim of this study was to investigate the influences of propofol on myocardial ischemia/reperfusion injury in rats through the Janus kinase/signal transducers and the activators of transcription (JAK/STAT) signaling pathway. MATERIALS AND METHODS:A total of 48 Sprague-Dawley (SD) rats were randomly divide...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201907_18456

    authors: Chen X,Wang Y,Xiao ZY,Hou DN,Li DB,Zhang XP

    更新日期:2019-07-01 00:00:00

  • Serum biomarkers in the diagnosis of periprosthetic joint infection: consolidated evidence and recent developments.

    abstract::Periprosthetic Joint Infection (PJI) represents one of the leading causes of revision prosthetic surgery, accounting for 25% of failed Total Knee Replacement (TKR) and 15% of failed Total Hip Replacement (THR). The search for a biomarker that, together with clinical and radiological findings, could improve the managem...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章,评审

    doi:10.26355/eurrev_201904_17473

    authors: Vicenti G,Bizzoca D,Nappi V,Pesce V,Solarino G,Carrozzo M,Moretti F,Dicuonzo F,Moretti B

    更新日期:2019-04-01 00:00:00

  • Pulsatile antagonism on bradykinin 2-receptor (BK2R) by icatibant triggers the most effective kinin-dependent post-conditioning on rat hearts.

    abstract:OBJECTIVE:Pharmacological post-conditioning (PC) by intermittent but not continuous administration of exogenous bradykinin (BK) reduces ischemia/reperfusion (I/R) injury via the Reperfusion Injury Salvage Kinase (RISK) pathway activation. We evaluated whether intermittent administration with icatibant (HOE140), a BK2R ...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201905_17953

    authors: Sgarra L,Coco C,Montagnani M,Potenza MA

    更新日期:2019-05-01 00:00:00

  • MicroRNA-21-5p promotes the inflammatory response after spinal cord injury by targeting PLAG1.

    abstract:OBJECTIVE:The aim of this study was to investigate the expression level of microRNA-21-5p and its target gene PLAG1 in the serum of patients with spinal cord injury (SCI), as well as the role and mechanism of microRNA-21-5p in lipopolysaccharide-induced primary microglial inflammation. PATIENTS AND METHODS:The levels ...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202006_21480

    authors: Han K,Wang FR,Yu MQ

    更新日期:2020-06-01 00:00:00

  • 3D cone beam (CBCT) in evaluation of frontal recess: findings in youth population.

    abstract:BACKGROUND:Frontal recess is the anatomical region most difficult to manage in endoscopic frontal sinus surgery due to the extreme variability of the cell patterns that may be observed in this area. CT has always been the gold standard in preoperative evaluation, but especially in the assessment of the causes of fronta...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Minni A,Messineo D,Attanasio G,Pianura E,D'Ambrosio F

    更新日期:2012-07-01 00:00:00

  • Iloprost administration in acrodermatitis of Hallopeau complicated by acquired toes syndactyly: a case report and review of the literature.

    abstract:OBJECTIVE:Acrodermatitis Continua of Hallopeau (ACH) is a variant of pustular psoriasis often very difficult to treat. Secondary syndactyly, also called "pseudosyndactyly", is rare and can be a complication of burns, dystrophic epidermolysis bullosa or trauma. If left untreated, joint complications and definitive funct...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章,评审

    doi:

    authors: Segreto F,Tosi D,Marangi GF,Pendolino AL,Santoro S,Gigliofiorito P,Persichetti P

    更新日期:2015-08-01 00:00:00

  • Correlations of degree of coronary artery stenosis with blood lipid, CRP, Hcy, GGT, SCD36 and fibrinogen levels in elderly patients with coronary heart disease.

    abstract:OBJECTIVE:To explore the correlations between the degree of coronary artery stenosis with blood lipid, C-reactive protein (CRP), homocysteine (Hcy), gamma-glutamyl transpeptidase (GGT), soluble cluster determinant 36 (sCD36), and fibrinogen (Fib) levels in elderly patients with coronary heart disease. PATIENTS AND MET...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201911_19453

    authors: Han K,Lu Q,Zhu WJ,Wang TZ,Du Y,Bai L

    更新日期:2019-11-01 00:00:00

  • LncRNALUADT1 is overexpressed in colorectal cancer and its expression level is related to clinicopathology.

    abstract:OBJECTIVE:LncRNAs participate in the proliferation, apoptosis, and invasion of colorectal cancer. We aimed at investigating the uncovered effect of lncRNALUADT1 on colorectal cancer. PATIENTS AND METHODS:The relative expression level of lncRNALUADT1 in tumor specimen was tested by Real-time quantitative PCR. The assoc...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201804_14816

    authors: Zhang XF,Zhang Y,Shen Z,Yang GG,Wang HD,Li LF,Liu DC,Qiu JM

    更新日期:2018-04-01 00:00:00

  • Long noncoding RNA GIHCG is a potential diagnostic and prognostic biomarker and therapeutic target for renal cell carcinoma.

    abstract:OBJECTIVE:Long noncoding RNA (lncRNA) GIHCG has been reported as an oncogene in hepatocellular carcinoma. However, the expression, roles, and clinical values of GIHCG in renal cell carcinoma (RCC) remain unclear. The aim of this study was to investigate the expression, roles, diagnostic and prognostic values of GIHCG i...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201801_14099

    authors: He ZH,Qin XH,Zhang XL,Yi JW,Han JY

    更新日期:2018-01-01 00:00:00

  • Association between hsa_circ_0006156 expression and incidence of gastric cancer.

    abstract:OBJECTIVE:To screen the differentially expressed circular ribonucleic acids (circRNAs) related to gastric cancer and to explore their associations with the clinicopathological features of gastric cancer. PATIENTS AND METHODS:Cancer tissues of 50 gastric cancer patients undergoing surgical resection in our hospital fro...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202003_20667

    authors: He YX,Ju H,Li N,Jiang YF,Zhao WJ,Song TT,Ren WH

    更新日期:2020-03-01 00:00:00

  • Role of TNF-alpha polymorphism in patients with nickel allergy: a marker of susceptibility to contact polysensitization.

    abstract:OBJECTIVE:Nickel allergy is the most frequent contact allergy in the industrialized country. In allergic contact dermatitis after the presentation of haptenated peptides by resident or newly recruited skin cells, activated CD8+ T cells release IFN-γ and TNF-α, these cytokines are potent activator of keratinocytes. The ...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Colagiovanni A,Di Renzo L,Sarlo F,Schiavino D,De Lorenzo A

    更新日期:2016-06-01 00:00:00

  • MiR-155-5p accelerates cerebral ischemia-reperfusion injury via targeting DUSP14 by regulating NF-κB and MAPKs signaling pathways.

    abstract:OBJECTIVE:This study aimed to explore the role of miR-155-5p in middle cerebral artery occlusion/reperfusion (MCAO/R) model in rats and oxygen-glucose deprivation/reoxygenation (OGD/R)-induced SH-SY5Y cells. In addition, this study also aimed to explore the underlying mechanisms to expect that miR-155-5p may be investi...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202002_20198

    authors: Shi Y,Li K,Xu K,Liu QH

    更新日期:2020-02-01 00:00:00

  • MiR-490-3p inhibited the proliferation and metastasis of esophageal squamous cell carcinoma by targeting HMGA2.

    abstract:OBJECTIVE:To identify the potential role of miR-490-3p in the development of esophageal squamous cell carcinoma (ESCC), and to explore the possible underlying mechanism. PATIENTS AND METHODS:Human ESCC tissues and cancer-adjacent normal tissues were collected. The mRNA expression level of miR-490-3p was detected by qu...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201812_16527

    authors: Kang NN,Ge SL,Zhang RQ,Huang YL,Liu SD,Wu KM

    更新日期:2018-12-01 00:00:00

  • Circ_0009910 promotes proliferation and metastasis of hepatocellular carcinoma cells through miR-335-5p/ROCK1 axis.

    abstract:OBJECTIVE:CircRNAs serve an essential role in regulating the development and progression of various tumors. The aim of this study was to examine the role and mechanism of circ_0009910 in hepatocellular carcinoma (HCC). MATERIALS AND METHODS:RT-qPCR was used to detect the expression of circ_0009910 and miR-335-5p in ti...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202002_20349

    authors: Li HW,Liu J

    更新日期:2020-02-01 00:00:00

  • IPO5 promotes malignant progression of esophageal cancer through activating MMP7.

    abstract:OBJECTIVE:Previous studies have found that IPO5 is a cancer-promoting gene. However, the role of IPO5 in esophageal cancer has not been reported. This study aims to investigate the expression characteristics of IPO5 in esophageal cancer, and to further analyze its relationship with clinical parameters and prognosis of ...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202004_21004

    authors: Li XF,Aierken AL,Shen L

    更新日期:2020-04-01 00:00:00

  • Effect of mangosteen (Garcinia mangostana L.) extracts as a feed additive on growth and hematological parameters of African catfish (Clarias gariepinus) fingerlings.

    abstract:AIM:The efficacy of dietary inclusion of various parts of mangosteen (Garcinia mangostana L.) extract on growth and hematological parameters of African catfish (Clarias gariepinus) fingerlings were investigated. MATERIAL AND METHODS:Ninety six fingerlings were randomly distributed into each of ten 20L plastic aquaria....

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Soosean C,Marimuthu K,Sudhakaran S,Xavier R

    更新日期:2010-07-01 00:00:00

  • Ischemic preconditioning protects brain from ischemia/reperfusion injury by attenuating endoplasmic reticulum stress-induced apoptosis through PERK pathway.

    abstract:OBJECTIVE:The purpose of this study was to explore the effects of cerebral ischemic preconditioning which can decrease brain ischemia/reperfusion (I/R) injury by inhibiting endoplasmic reticulum (ER) stress-induced apoptosis. MATERIALS AND METHODS:The focal cerebral ischemia rat was selected as the experimental model....

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201712_14020

    authors: Hu YQ,Chen W,Yan MH,Lai JJ,Tang N,Wu L

    更新日期:2017-12-01 00:00:00

  • MiR-221 affects proliferation and apoptosis of gastric cancer cells through targeting SOCS3.

    abstract::Since this article has been suspected of research misconduct and the corresponding authors did not respond to our request to prove originality of data and figures, "MiR-221 affects proliferation and apoptosis of gastric cancer cells through targeting SOCS3, by Q.-Y. Zhou, P.-L. Peng, Y.-H. Xu, published in Eur Rev Med...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202007_22226

    authors: Zhou QY,Peng PL,Xu YH

    更新日期:2020-07-01 00:00:00

  • LncRNA DCST1-AS1 accelerates the proliferation, metastasis and autophagy of hepatocellular carcinoma cell by AKT/mTOR signaling pathways.

    abstract:OBJECTIVE:The previous research revealed that long noncoding RNAs (lncRNAs) play a vital role in the development of hepatocellular carcinoma (HCC). To further discuss the underlying mechanisms of lncRNA DCST1-AS1 in the pathogenesis of HCC. PATIENTS AND METHODS:We screened the abnormally expressed genes in HCC tissues...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201907_18423

    authors: Li J,Zhai DS,Huang Q,Chen HL,Zhang Z,Tan QF

    更新日期:2019-07-01 00:00:00

  • Use of Eribulin mesylate as second-line therapy in elderly patients with HER/2 negative metastatic breast cancer (MBC): efficacy, tolerability and Quality of Life.

    abstract:OBJECTIVE:Eribulin mesylate (Halaven®) is a non-taxane inhibitor of microtubule indicated as monotherapy in patients with metastatic breast cancer (MBC), which progresses after anthracycline and taxanes therapy. In this retrospective observational study, we want to evaluate the efficacy of Eribulin in elderly women wit...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202012_24171

    authors: De Luca R,Alù M,Genova G,Grassadonia A,Cicero G

    更新日期:2020-12-01 00:00:00

  • LINC01198 promotes colorectal cancer cell proliferation and inhibits apoptosis via Notch signaling pathway.

    abstract:OBJECTIVE:To detect the expression level of long intergenic non-protein coding RNA 1198 (LINC01198) in colorectal cancer (CRC) tissues and cells, to investigate the effect of LINC01198 on the biological function of CRC cells through in vivo and in vitro experiments, and to explore its molecular mechanism. PATIENTS AND...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202008_22641

    authors: Chen S,Yuan H,Chen BC,Wan ZA,Tu SL,Hu XY

    更新日期:2020-08-01 00:00:00

  • The role of nuclear medicine in the diagnosis of spondylodiscitis.

    abstract:BACKGROUND:The diagnosis of spondylodiscitis can be difficult, because the patients history, subjective symptoms and physical findings are often inconclusive, particularly in the early stages. AIM:To perform an overview on the role of nuclear medicine procedures with single photon emission tomography (SPET) and positr...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章,评审

    doi:

    authors: Treglia G,Focacci C,Caldarella C,Mattoli MV,Salsano M,Taralli S,Giordano A

    更新日期:2012-04-01 00:00:00

  • Electroionotherapy in acute arthrorheumatic pain.

    abstract::The authors evaluated the efficacy of an electronic treatment for pain, using an ion flow generator, BE-101 model by Bio-Ejt, on 19 patients suffering from acute pain of an arthrorheumatic nature. Each patient was treated for two weeks every other day (6 sittings), each sitting lasting 20 minutes at an intensity of ab...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 临床试验,杂志文章

    doi:

    authors: Maugeri D,Russo MS,Bonanno MR,Curasí MP,Speciale S,Santangelo A,Panebianco P,Malaguarnera M

    更新日期:1999-03-01 00:00:00

  • Significance of LncRNA KCNQ1OT1 expression in diagnosis and prognosis judgment of myelodysplastic syndrome.

    abstract:OBJECTIVE:To study the expression level of the long non-coding ribonucleic acid (lncRNA) KCNQ1 overlapping transcript 1 (KCNQ1OT1) in the patients with myelodysplastic syndrome (MDS), as well as the correlation between the expression level of lncRNA KCNQ1OT1 and the clinical diagnosis and prognosis of MDS. PATIENTS AN...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202005_21341

    authors: Zhang SF,Jin L,Chen YF

    更新日期:2020-05-01 00:00:00

  • CHARGE syndrome: an overview on dental and maxillofacial features.

    abstract:OBJECTIVES:CHARGE is an acronym referring to the aspects of this rare syndromic condition. Patients with CHARGE association are today considered as subjects lacking in pathognomonic dental alterations. The present study is aimed at adding to the body of evidence of the cases reported in literature and the continuous cl...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Inchingolo F,Pacifici A,Gargari M,Acitores Garcia JI,Amantea M,Marrelli M,Dipalma G,Inchingolo AM,Rinaldi R,Inchingolo AD,Pacifici L,Tatullo M

    更新日期:2014-01-01 00:00:00

  • Resveratrol protects against oxidative damage of retinal pigment epithelium cells by modulating SOD/MDA activity and activating Bcl-2 expression.

    abstract:OBJECTIVE:Age-related macular degeneration (AMD) is mainly characterized by dysfunction of retinal pigment epithelium (RPE) cells. This study aimed to investigate the protective effects of resveratrol on oxidative damaged RPE cells. MATERIALS AND METHODS:Human D407 cells were divided into normal control (NC), H2O2 tre...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201901_16786

    authors: Yang Y,Wu ZZ,Cheng YL,Lin W,Qu C

    更新日期:2019-01-01 00:00:00

  • Renoprotective activity of telmisartan versus pioglitazone on ischemia/reperfusion induced renal damage in diabetic rats.

    abstract:OBJECTIVES:Diabetes mellitus (DM) causes organ dysfunction and increases the sensitivity of organs to damages.To test this hypothesis, we used renal ischemia/reperfusion (I/R) experiment to evaluate the renoprotective activity of telmisartan versus pioglitazone on I/R induced renal damage in diabetic rats. MATERIALS A...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Tawfik MK

    更新日期:2012-05-01 00:00:00

  • Bioinformatics analysis of common differential genes of coronary artery disease and ischemic cardiomyopathy.

    abstract:OBJECTIVE:This paper aims at screening the common differential genes of coronary atherosclerotic heart disease (CAD) and ischemic cardiomyopathy (ICM), and to conduct pathway analysis and protein-protein interaction (PPI) network analysis for the differential genes. MATERIALS AND METHODS:The CAD and ICM datasets were ...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201806_15182

    authors: Li GM,Zhang CL,Rui RP,Sun B,Guo W

    更新日期:2018-06-01 00:00:00

  • Ipriflavone promotes osteogenesis of MSCs derived from osteoporotic rats.

    abstract:OBJECTIVE:To explore whether Ipriflavone could prevent postmenopausal osteoporosis (PMOP) and improve bone quality via promoting osteogenesis of bone marrow-derived mesenchymal stem cell (MSCs). MATERIALS AND METHODS:MSCs were extracted from rats and identified using flow cytometry. Osteogenic specific genes and adipo...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201807_15527

    authors: Gao AG,Zhou YC,Hu ZJ,Lu BB

    更新日期:2018-07-01 00:00:00

  • Sivelestat sodium hydrate attenuates acute lung injury by decreasing systemic inflammation in a rat model of severe burns.

    abstract:OBJECTIVE:Patients with severe burns often develop acute lung injury (ALI), systemic inflammatory response syndrome (SIRS) often complicates with ALI. Sivelestat sodium hydrate is an effective drug against ALI. However, the mechanisms of this beneficial effect are still poorly understood. In the current study, we evalu...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Xiao XG,Zu HG,Li QG,Huang P

    更新日期:2016-01-01 00:00:00