Abstract：
:The aim of this study was to investigate the effect of divalent metal ions (Ca, Mg(2+) , and Zn(2+) ) on the stability of oxytocin in aspartate buffer (pH 4.5) and to determine their interaction with the peptide in aqueous solution. Reversed-phase high-performance liquid chromatography and high-performance size-exclusion chromatography measurements indicated that after 4 weeks of storage at 55°C, all tested divalent metal ions improved the stability of oxytocin in aspartate-buffered solutions (pH 4.5). However, the stabilizing effects of Zn(2+) were by far superior compared with Ca(2+) and Mg(2+) . Liquid chromatography-tandem mass spectrometry showed that the combination of aspartate and Zn(2+) in particular suppressed the formation of peptide dimers. As shown by isothermal titration calorimetry, Zn(2+) interacted with oxytocin in the presence of aspartate buffer, whereas Ca(2+) or Mg(2+) did not. In conclusion, the stability of oxytocin in the aspartate-buffered solution is strongly improved in the presence of Zn(2+) , and the stabilization effect is correlated with the ability of the divalent metal ions in aspartate buffer to interact with oxytocin. The reported results are discussed in relation to the possible mode of interactions among the peptide, Zn(2+) , and buffer components leading to the observed stabilization effects.
journal_name
J Pharm Scijournal_title
Journal of pharmaceutical sciencesauthors
Avanti C,Hinrichs WLJ,Casini A,Eissens AC,Van Dam A,Kedrov A,Driessen AJM,Frijlink HW,Permentier HPdoi
10.1002/jps.23546subject
Has Abstractpub_date
2013-06-01 00:00:00pages
1734-1741issue
6eissn
0022-3549issn
1520-6017pii
S0022-3549(15)31084-4journal_volume
102pub_type
杂志文章abstract：:Trimethoprim undergoes thermally and photochemically catalyzed hydrolysis or oxidation to give rise to at least five products. The structures of these compounds were determined by physical methods and it was found that the resonance of C-5 in the 13C NMR spectrum is indicative of the substitution pattern of the result...
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pub_type： 临床试验,杂志文章
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更新日期：2000-08-01 00:00:00
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更新日期：1995-02-01 00:00:00
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更新日期：2014-04-01 00:00:00
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更新日期：1980-11-01 00:00:00
