High-performance liquid chromatographic determination of 4-amino-5-chloro-N-[(1-ethylimidazolin-2-yl)methyl]-2-methoxybenzamide and its metabolite in biological fluids. Applications in pharmacokinetic studies.


:A high-performance liquid chromatographic method with spectrofluorometric detection has been developed for the analysis of a new substituted benzamide and its metabolite in body fluids. The detection was performed at 270 nm for excitation, and 370 nm for emission. The proposed technique is selective, reliable, and sensitive. The detection limit is 1 ng/mL for the parent drug and 2 ng/mL for the imidazole derivative. The reproducibility of the analytical method through statistical coefficients is approximately 5%. The accuracy of the method is good; that is, the relative error is less than 2%. To validate this method, the stability of these two drugs was tested at the time of conservation of urine and plasma samples and during all steps of the analytical method.


J Pharm Sci


Bressolle F,Bres J,Moulin A




Has Abstract


1990-06-01 00:00:00














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    journal_title:Journal of pharmaceutical sciences

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    authors: Trapani G,Franco M,Trapani A,Lopedota A,Latrofa A,Gallucci E,Micelli S,Liso G

    更新日期:2004-12-01 00:00:00

  • Application of one-phase end-point change system in two-phase titration to amine drug analysis.

    abstract::A titration method was developed for the determination of diphenhydramine, quinine, neostigmine, sparteine, strychnine, homatropine, atropine, physostigmine, and procaine in aqueous solution. Tetraphenylborate was used as a titrant with tetrabromophenolphthalein ethyl ester as an indicator in the presence of organic s...

    journal_title:Journal of pharmaceutical sciences

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    authors: Tsubouchi M,Mitsushio H,Yamasaki N,Matsuoka K

    更新日期:1981-11-01 00:00:00

  • Oil-frozen W₁/O/W₂ double emulsions for dermal biomacromolecular delivery containing ethanol as chemical penetration enhancer.

    abstract::Oil-frozen water-in-oil-in-water (W₁/O/W₂) double emulsions (DE) containing ethanol up to 40% (w/v) in the external aqueous W₂ phase exhibited external coalescence upon thawing of the oil phase, releasing up to 85% of the encapsulated protein of the internal aqueous phase. These emulsions were studied in vitro as pote...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Jaimes-Lizcano YA,Lawson LB,Papadopoulos KD

    更新日期:2011-04-01 00:00:00

  • Synthesis and preliminary pharmacology of an internal standard for assay of neostigmine.

    abstract::The synthesis of the diethyl analog of neostigmine, its preliminary pharmacology, and its use as an internal standard for the GLC assay of neostigmine are described. Both the diethyl analog and neostigmine undergo thermal demethylation in the injection port. The column selected produced satisfactory resolution and sho...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Ward HE Jr,Freeman JJ,Sowell JW,Kosh JW

    更新日期:1981-04-01 00:00:00

  • Antibody response to aggregated human interferon alpha2b in wild-type and transgenic immune tolerant mice depends on type and level of aggregation.

    abstract::The aim of this study was to determine the sensitivity of transgenic immune tolerant mice for the type and level of aggregation of recombinant human interferon alpha2b (rhIFNalpha2b). RhIFNalpha2b was aggregated by metal-catalyzed oxidation or by incubation at elevated temperature and various pHs. Native rhIFNalpha2b ...

    journal_title:Journal of pharmaceutical sciences

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    authors: Hermeling S,Schellekens H,Maas C,Gebbink MF,Crommelin DJ,Jiskoot W

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  • Development of budesonide nanocluster dry powder aerosols: processing.

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    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: El-Gendy N,Selvam P,Soni P,Berkland C

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  • Determination of captopril in human blood and urine by GLC-selected ion monitoring mass spectrometry after oral coadministration with its isotopomer.

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    authors: Ocal Y,Kurum B,Karahan S,Tezcaner A,Ozen S,Keskin D

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  • Physicochemical, crystallographic, thermal, and spectroscopic behavior of crystalline and X-ray amorphous ciclesonide.

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    authors: Feth MP,Volz J,Hess U,Sturm E,Hummel RP

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  • Ionophoric properties of polysorbate 80.

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  • Impact of curcumin supersaturation in antibacterial photodynamic therapy--effect of cyclodextrin type and amount: studies on curcumin and curcuminoides XLV.

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    authors: Hegge AB,Nielsen TT,Larsen KL,Bruzell E,Tønnesen HH

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  • Modeling of the Biological Activity of Monoclonal Antibodies Based on the Glycosylation Profile.

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    authors: Bartusik-Czubek E,Toboła P,Czubek B,Bednarek M,Balcerek J,Pietrucha T,Jaros S

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  • Stability of tetrahydrocannabinols II.

    abstract::The biphasic degradation of delta9-tetrahydrocannabinol (I), as monitored by flame-ionization GLC, produced delta8-tetrahydrocannabinol (II), cannabidiol (X), 9-hydroxyhexahydrocannabinol (VI), 9,10-dihydro-9-hydroxyisocannabidiol (VI), and and 6,12-dihydro-6-hydroxycannabidiol (VIII) in acidic solutions. Further iden...

    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Garrett ER,Gouyette AJ,Roseboom H

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    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: de Armas HN,Peeters OM,Van den Mooter G,Blaton N

    更新日期:2007-05-01 00:00:00

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    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Kyyrönen K,Urtti A

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  • Physical characterization of clostridium difficile toxins and toxoids: effect of the formaldehyde crosslinking on thermal stability.

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    pub_type: 杂志文章


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    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Chafetz L,Desai MP,Sukonik L

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    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Nolting A,Costa TD,Vistelle R,Rand KH,Derendorf H

    更新日期:1996-04-01 00:00:00

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    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Schote U,Ganz P,Fahr A,Seelig J

    更新日期:2002-03-01 00:00:00

  • In vivo evaluation of controlled-release products.

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    journal_title:Journal of pharmaceutical sciences

    pub_type: 杂志文章


    authors: Hwang SS,Bayne W,Theeuwes F

    更新日期:1993-11-01 00:00:00