Abstract：
:Stem cell therapy is a promising strategy for overcoming the limitations of current treatment methods. The modification of stem cell properties may be necessary to fully exploit their potential. Genetic engineering, with an abundance of methodology to induce gene expression in a precise and well-controllable manner, is particularly attractive for this purpose. There are virus-based and non-viral methods of genetic manipulation. Genome-integrating viral vectors are usually characterized by highly efficient and long-term transgene expression, at a cost of safety. Non-integrating viruses are also highly efficient in transduction, and, while safer, offer only a limited duration of transgene expression. There is a great diversity of transfectable forms of nucleic acids; however, for efficient shuttling across cell membranes, additional manipulation is required. Both physical and chemical methods have been employed for this purpose. Stem cell engineering for clinical applications is still in its infancy and requires further research. There are two main strategies for inducing transgene expression in therapeutic cells: transient and permanent expression. In many cases, including stem cell trafficking and using cell therapy for the treatment of rapid-onset disease with a short healing process, transient transgene expression may be a sufficient and optimal approach. For that purpose, mRNA-based methods seem ideally suited, as they are characterized by a rapid, highly efficient transfection, with outstanding safety. Permanent transgene expression is primarily based on the application of viral vectors, and, due to safety concerns, these methods are more challenging. There is active, ongoing research toward the development of non-viral methods that would induce permanent expression, such as transposons and mammalian artificial chromosomes.
journal_name
Acta Neurobiol Exp (Wars)journal_title
Acta neurobiologiae experimentalisauthors
Nowakowski A,Andrzejewska A,Janowski M,Walczak P,Lukomska Bsubject
Has Abstractpub_date
2013-01-01 00:00:00pages
1-18issue
1eissn
0065-1400issn
1689-0035pii
7301journal_volume
73pub_type
杂志文章,评审abstract：:As investigation has been made of the adequacy of direct current to block conduction in the myelinated fibres of the cervical vagus nerve, leaving the nonmyelinated fibres conducting normally. Conduction was assessed from the compound electroneurograms, and from single fibre recording. Conduction of spontaneous activi...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1973-01-01 00:00:00
abstract：:Our work assesses the effects of mu opioid receptor activation on high-threshold Ca2+/Ba2+ currents in freshly dispersed pyramidal neurons of the medial prefrontal cortex in rats. Application of the specific mu receptor agonist (D-Ala2+, N-Me-Phe4+, Gly5+-ol)-enkephalin (DAMGO) at 1 microM decreased Ca2+ current ampli...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Earlier in vivo studies conducted on freely moving and anesthetized rats demonstrated that the posterior hypothalamus (PH) comprises pathways critical for producing the synchronous hippocampal formation (HPC) theta rhythm. In addition, these findings suggested that the frequency of the HPC theta was encoded in the PH ...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Temporal information processing controls many aspects of human mental activity and may be assessed by examining perception of temporal order in the tens of milliseconds time range. Although existing studies suggest an age-related decline in mental abilities, the data on the deterioration of temporal order perception s...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:The involvement of calcium ions in mechanisms of ischemic brain injury has been suggested for several years. Our understanding of the role of intracellular Ca2+ as a trigger of acute neurotoxicity and in the induction of long lasting processes leading to necrotic and/or apoptotic postischemic delayed neuronal death or...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：1996-01-01 00:00:00
abstract：:Proprioceptive effects (PE) on directional hearing, i.e., sound image shifts relatively to the sound source, produced by changes in head position, were studied in their dependence on head movement type in two groups of listeners. The first group included subjects in whom horizontal head deviation to the right or to th...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Spontaneous and evoked activity of red nucleus neurons is described in chloralose anesthetized cats. It is suggested that some effects, e.g. burst discharges and prolonged inhibitory-excitatory responses, were induced by the use of chloralose. ...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:A differentiation of instrumental responses to auditory location stimuli using the Konorski Test was investigated. Each stimulus consisted of two components separated by intrastimulus interval. The components were click series presented from four loudspeakers situated in front of the animal, on horizontal and vertical...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:Due to technological advances in electrophysiology, there is renewed interest in the analysis of local field potentials recorded at many sites simultaneously. In this paper the main problems related to the analysis of LFP are presented, and recent developments in the data analysis methods are reviewed. The focus of th...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：2015-01-01 00:00:00
abstract：:Deprivation of patterned visual information, as in early onset congenital cataract patients, results in a severe impairment in global motion perception. Previously we reported a delayed maturation of the peripheral visual field representation in primary visual area 17, based on a 2-D DIGE screen for protein expression...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2017-01-01 00:00:00
abstract：:New features of actual choice behavior and effortful information processing in rats were demonstrated in an eight-arm radial maze through modifications of a matching-to-sample task. Two attempts were allowed for a squad of hooded Sprague-Dawley rats (n=7) for finding a reward in a testing phase of the task. The result...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:The effects of unilateral ablation of the cortical motor forelimb area on instrumental avoidance responses consisting of lifting the forelimb and holding it within a 4 cm wide "safety zone" were studied in dogs. It was found that the surgery irreversibly impaired the search for the safety zone, while the ability to ma...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:Preconditioning is an experimental strategy for reducing ischemic brain damage. There are reports that brief exposure of neurons to NMDA-receptor antagonists may be an adequate preconditioning stressor. We studied effects of preconditioning of the cerebellar granule cells (CGC) in primary culture by 30-minute exposure...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:The relationship between neocortical high voltage spindle activity (HVS) and the level of arousal (state of vigilance) was investigated in imp-DAK rats. Two groups of single records (6 h and 24 h) and one group of repeated ones (6 h daily for three consecutive days) were analysed. Five levels of arousal (states) were ...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Computer techniques were employed to construct a respiratory control model in which the variables treated were programmed according to a series of mathematical equations, with the purpose of eliciting the relationship of V-PCO2 (minute ventilation-partial pressure of carbon dioxide) on a transient time basis. It was d...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1973-01-01 00:00:00
abstract：:During paradoxical sleep electrical stimulation of the mesencephalic reticular formation and emotiogenic structures in the mesencephalon and diencephalon produced depression or an increase in the theta rhythm of the hippocampal and entorhinal electrical activity. However, stimulation not involving behavioral arousal d...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1975-01-01 00:00:00
abstract：:Discrimination learning of instrumental responses to auditory compound stimuli was investigated in opossums using the R-no R (go-no go) differentiation. Each compound stimulus consisted of two factors: quality and location. Each correct response performed to the conditioned positive, or "safe" stimulus, was rewarded b...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Poly (lactide‑co‑glycolide) (PLGA) nanoparticles (NPs) are biodegradable carriers that participate in the transport of neuroprotective drugs across the blood brain barrier (BBB). Targeted brain‑derived neurotrophic factor (BDNF) delivery across the BBB could provide neuroprotection in brain injury. We tested the neuro...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2020-01-01 00:00:00
abstract：:During the normal course of neuronal differentiation gangliosides undergo marked changes in both quantity and quality. These changes, i.e. several-fold increase in concentration and appearance of the gangliotetraose family, have been observed both in vivo and in neuronal cell cultures. In addition to these naturally o...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章,评审
doi：
更新日期：1990-01-01 00:00:00
abstract：:Incubation of the acutely dissected rat hippocampal slices in calcium-containing media resulted in spontaneous activation-translocation of classical PKC isoforms and their subsequent (especially gamma-type) proteolytic degradation. These changes were blocked by calpain inhibitor MDL 28 170 in 100 microM concentration....
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:For years, interneurons expressing vasoactive intestinal peptide (VIP) interneurons and their function within the neocortex have been shrouded in mystery. Their relatively small size and minimal representation in the cortex have made investigation difficult. Due to their service role performed in co‑operation with gli...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2019-01-01 00:00:00
abstract：:The hippocampal EEG activity was recorded in male rats during the response-contingent reinforcement of a female. The instrumental response was a bar-pressing on a fixed-ratio-10 reinforcement schedule. The frequency of bar-pressing tends to increase during the succeeding copulatory series. The similar phenomenon was o...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:Adult, male, Long-Evans hooded rats were subjected to a spinal cord transection in the mid-thoracic region. Following surgery, animals were divided into groups for treatments with ACTH, Piromen, isobutyl-2-cyanoacrylate and Cytoxan. Animals were sacrificed at varying intervals from 7-180 days. Horizontal sections of t...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:The aim of the study was to evaluate REM sleep parameters, especially the temporal characteristics of rapid eye movement activity, in depressed patients, and to compare three different methods for scoring of REM density. The sleep of 15 nonmedicated depressed patients and 13 healthy controls was recorded during two co...
journal_title：Acta neurobiologiae experimentalis
pub_type： 临床试验,杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The high-affinity muscarinic antagonist L-[3H]quinuclidinyl benzi-late (L-[3H]QNB) was used to label muscarinic acetylcholine receptors in the astrocyte-enriched fraction isolated from rat hippocampus. Analysis of the saturation and drug inhibition data showed that [3H]QNB binding in the astroglia fraction was saturab...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:A new hypothesis concerning the mechanism of the P. rufescens' slave-raids, is proposed. (i) The so-called pseudo-scouting does not contribute to the slave-raids. (ii) The direction of the raid is determined by the random movement of the most easily aroused individuals - the so-called activists. (iii) Once the directi...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:We have studied MHC class II antigen expression and lymphocytic infiltration during dopaminergic neurone degeneration produced by intoxication with 1-methyl-4-phenyl-1,2,3,6-tetrahydropiridine (MPTP). Microglial activation was observed in the striatum and in the substantia nigra (SN) in this model. We noticed a marked...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The influence of bilateral injections of serotonin (5-HT) and methysergide (MET) to antero-medial hypothalamus (HA) on carbachol-induced emotional-defensive behavior was investigated. Earlier (15 min) stimulation of 5-HT receptors in HA region by 5-HT injections evoked a decrease in the level of vocal response (number...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1985-01-01 00:00:00
abstract：:The objective was to assess changes in the width of the subarachnoid space (SAS) and amplitude of cerebrovascular pulsation (CVP) during acute elevation of intracranial pressure (ICP) using Near Infrared Transillumination/Back Scattering Sounding (NIR-T/BSS). Changes in the width of the SAS and amplitude of CVP were o...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：:Connnections of the cortical region situated in the medial and lateral walls of the presylvian fissure in the dog were investigated using the method of retrograde HRP transport. The patterns of subcortico-cortical and cortico-cortical projections sho,w that the cortical field of the medial presylvian wall (paraorbital...
journal_title：Acta neurobiologiae experimentalis
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
