Abstract：
:Flow cytometry is used in the analysis of the multi-parameter optical properties of individual particles such as eukaryotic cells, prokaryotic cells, and viruses in the flow system. Virions, or complexes consisting of virus particles attached to the specific antibody in suspension are individually arranged in a linear stream, which flows through the detection device. The parameters measured by the flow cytometer include the volume of the particles or cells, the morphological complexity, the presence of pigments, RNA content, virion surface markers, and enzymatic activity. It is possible to collect two morphological parameters and one or more signals of the fluorescence of a single particle. Multi-parameter analysis provides for the definition a population of cells based on their phenotype. Flow cytometry is characterized by the automatic determination of the value of the parameter set for a large number of individual particles or cells in the course of each measurement. For example, 100,000 or more particles such as virus, bacteria, or fungal spores are analyzed one after another typically over a period of 1 min. The limit of detection in such studies is 100 fluorescing particles per cell. Theoretically, in the case of the influenza virus, this will be one copy of the virion combined in a complex with specific antibodies and with a built-in fluorescent label.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Wozniak-Kosek A,Brydak LBdoi
10.1007/978-94-007-6627-3_10subject
Has Abstractpub_date
2013-01-01 00:00:00pages
65-70eissn
0065-2598issn
2214-8019journal_volume
788pub_type
杂志文章abstract：:Human heart rate variability (HRV) was examined during a mild respiratory acidosis induced by inhalation of a normoxic hypercapnic gas mixture. On two separate occasions, separated by 72 but not more than 120hr, ECG was recorded from 9 normotensive subjects during supine rest. ECG was recorded for 20 min breathing roo...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-0-387-73693-8_63
更新日期：2008-01-01 00:00:00
abstract：:Mouse embryonic stem cells (mESCs) release into the medium in which they are cultured heterogeneous populations of microvesicles (mESMVs), important components of cell-cell communication, that transfer their contents not only to other stem cells but also to cells of other origins. The purpose of these studies was to d...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-319-17121-0_75
更新日期：2016-01-01 00:00:00
abstract：:A virus inducing a cytopathic effect on porcine alveolar macrophages was isolated from the lungs of a pig with respiratory problems and lesions of proliferative and necrotizing pneumonia. The isolate was found to react with porcine reproductive and respiratory syndrome virus (PRRSV) monoclonal antibodies by indirect i...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-1899-0_23
更新日期：1995-01-01 00:00:00
abstract：:In this brief review, I focused on the abdominal expiratory motor pattern in the rat. In the vagotomized adult rat, hypercapnic acidosis evoked two patterns of the abdominal expiratory activity; one with low amplitude expiratory discharge (E-all activity) that persisted throughout the expiratory phase, and another wit...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-5692-7_31
更新日期：2010-01-01 00:00:00
abstract：:Since many aspects of schizophrenia are also manifested at the peripheral level in proliferating cell types, this chapter describes the analysis of skin fibroblasts biopsied from living patients. The method focuses on cell culture and sample preparation for characterization of the model. The resulting cell extracts ca...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-319-52479-5_29
更新日期：2017-01-01 00:00:00
abstract：:The pattern of contaminants in pharmaceutical and feed grade L-tryptophan (Trp) was investigated in a market survey of 22 lots of 6 different manufacturers. To date, 5 case associated contaminants in Showa Denko tryptophan (SD-Trp) known to cause the autoimmune disease eosinophilia-myalgia syndrome (EMS) have been str...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4709-9_59
更新日期：1999-01-01 00:00:00
abstract：:ATP stimulates arachidonic acid release and prostaglandin biosynthesis (most likely via phospholipase A2 (PLA2) activation) and phospholipase C (PLC) activation in cultured rabbit coronary microvessel endothelial cells. Pertussis toxin pretreatment inhibits ATP stimulated prostaglandin release, but not ATP stimulated ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-5805-3_7
更新日期：1990-01-01 00:00:00
abstract：:Fructose malabsorption is characterized by the inability to absorb fructose efficiently. Consequently fructose reaches the colon and is broken down by bacteria to short-fatty-acids, CO2 and H2. Recently we found that fructose malabsorption was associated with signs of depression. It was therefore of interest to find o...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4709-9_9
更新日期：1999-01-01 00:00:00
abstract：:Mitochondria have long been known to be critical for cell survival due to their role in energy metabolism. However, not until the mid-1990s did it become evident that mitochondria are also active participants in programmed cell death (PCD). This chapter focuses mainly on the role the mitochondria in mammalian cell dea...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4020-6554-5_3
更新日期：2008-01-01 00:00:00
abstract：:There is no doubt that feeding and nutrient availability modify the metabolism through hormonal changes. It is otherwise interesting to better define the influence of the imposed or freely chosen meal distribution during the day; in fact the daily feed intake in a relatively short time, namely during the daylight, see...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/0-306-46832-8_27
更新日期：2000-01-01 00:00:00
abstract：:We have described our current criteria for selecting those patients with acute V T-E who merit long-term protection, for accomplishing the acute to long-term transition efficiently and for deciding when such protection can be interrupted. Naturally, in individual patients, special circumstances may exist which alter t...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4757-5985-3_17
更新日期：1987-01-01 00:00:00
abstract：:The European Directive 98/79/EC on in vitro diagnostic medical devices (IVD) regulates the marketing and post market surveillance of IVD in the European Economic Area. In cases of incidents and field corrective actions, the manufacturers have to inform the responsible Competent Authorities (CA). In Germany, the Federa...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-94-007-4546-9_48
更新日期：2013-01-01 00:00:00
abstract：:Macromolecular crystallography has been central to the emergence and development of structural biology as a scientific discipline. Approximately 90% of the more than 138,000 three-dimensional structures currently available in the Protein Data Bank (PDB) archive, the single, global open access data resource for macromo...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-13-2200-6_2
更新日期：2018-01-01 00:00:00
abstract：:Autoantibodies against neutrophil cytoplasmic antigens (ANCA) are found in patients with different kinds of systemic vasculitis and glomerulonephritis, both characteristic manifestations of systemic lupus erythematosus (SLE). The present study was designed to clarify the nature of ANCA seen in SLE patients and their c...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-9182-2_65
更新日期：1993-01-01 00:00:00
abstract：:The activation of the magnocellular oxytocin system by different physiological stimuli will require specific genomic responses that may or may not reflect the electrical and short-term secretory activity of the neurones. One of the main determinants of synthetic activity is the rate of transcription and this can be al...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Oxygen deprivation following cessation of blood flow to vital organs such as brain, heart, and kidney is a ubiquitous human disease, invariably leading to devastating consequences. Studies in experimental models support the contention that membrane permeability is altered, ion fluxes impaired, and energy stores deplet...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-3426-6_28
更新日期：1992-01-01 00:00:00
abstract：:Antigen 43 (Ag43) is a prominent hetero-oligomeric protein complex in the outer membrane of Escherichia coli. It is composed of two subunits. alpha 43 (M(r) 60, 000) and beta 43 (M(r) 53, 000) in 1:1 stoichiometry. alpha 43 is surface expressed, extends beyond the O-side chains of smooth lipopolysaccharide and is boun...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-1828-4_56
更新日期：1997-01-01 00:00:00
abstract：:We describe the events surrounding the identification of the exosome complex and the subsequent early development of the field. Like many scientific discoveries, the initial identification and characterization ofthe exosome was based on a combination of skill, good fortune--and the availability of cutting edge technol...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：
更新日期：2010-01-01 00:00:00
abstract：:Endocrine fibroblast growth factors (FGFs) have been recognized as hormones that regulate a variety of metabolic processes. FGF19 is secreted from intestine upon feeding and acts on liver to suppress bile acid synthesis. FGF21 is secreted from liver upon fasting and acts on adipose tissue to promote lipolysis and resp...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4614-0887-1_2
更新日期：2012-01-01 00:00:00
abstract：:Exposure to hyperoxia, especially under hyperbaric conditions, causes an enhanced oxidative stress particularly in lung tissue. To test the potential hazardous effect of either a single or repeated hyperbaric oxygen treatment (HBO) on the cellular defence system the glutathione status of lung tissue from rats exposed ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-3428-0_13
更新日期：1992-01-01 00:00:00
abstract：:Mobile health applications are steadily gaining momentum in the modern world given the omnipresence of various mobile or Wi-Fi connections. Given that the bandwidth of these connections increases over time, especially in conjunction with advanced modulation and error-correction codes, whereas the latency drops, the co...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-030-32622-7_2
更新日期：2020-01-01 00:00:00
abstract：:Streptococcus pneumoniae (Pnc) is one of the leading pathogens in the world. Attachment to respiratory mucosal and lung surfaces is presumed to be involved in carriage, in disease and in the interaction with macrophages initiating innate immune responses. We hypothesized that bacterial adhesins mediate Pnc adhesion an...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/0-306-46831-X_6
更新日期：2000-01-01 00:00:00
abstract：:Schizophrenia alters basic brain processes of perception, emotion, and judgment to cause hallucinations, delusions, thought disorder, and cognitive deficits. Unlike neurodegeneration diseases that have irreversible neuronal degeneration and death, schizophrenia lacks agreeable pathological hallmarks, which makes it on...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-7091-0932-8_22
更新日期：2012-01-01 00:00:00
abstract：:Flaxseed has been used as an edible grain in different parts of the world since ancient times. However, use of flaxseed oil has been limited due to its high content of polyunsaturated fatty acids. Nonetheless, alpha-linolenic acid, dietary fiber and lignans of flaxseed have regained attention. New varieties of flaxsee...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4899-1925-0_26
更新日期：1998-01-01 00:00:00
abstract：:Fumonisins, a group of mycotoxins produced by Fusarium moniliforme in corn, have been implicated in several animal and human diseases. F. moniliforme and the fumonisins are an area of incresing concern for corn producers and consumers. Consequently, there is interest in reducing human and animal exposure to these fung...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4899-1379-1_30
更新日期：1996-01-01 00:00:00
abstract：:Labetalol is a drug used in the treatment of hypertensive disorders of pregnancy (HDP). In a previous study we investigated the influence of the maternal use of labetalol on the cerebral autoregulation (CA) mechanism of neonates. In that study, we found that labetalol induces impaired CA during the first day of life, ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4939-0620-8_23
更新日期：2014-01-01 00:00:00
abstract：:Lymphocyte development and ontogenetic changes in erythroid cells have been studied in chick-chick yolk sac-embryo chimeras constructed of histoincompatible partners. The results obtained indicate that the early chick yolk sac produces transiently erythroid stem cells whereas definitive erythrocytes are derived from t...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-9066-4_2
更新日期：1982-01-01 00:00:00
abstract：:Taurine transporter (TauT) has been identified as a target gene of p53 tumor suppressor. TauT is also found to be overexpressed in variety type of human cancers, such as leukemia. This study showed that expression of TauT was upregulated by c-Myc and c-Jun oncogenes. To explore whether blocking of TauT inhibits tumor ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-981-13-8023-5_50
更新日期：2019-01-01 00:00:00
abstract：:Fertility preservation includes all the necessary steps that have to be taken in order to implement a woman's aim for preserving and enhancing her future chances of having her own biological offspring.There are two available choices for patients to maintain their fertility.The first category includes all the preventiv...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-41596-9_25
更新日期：2020-01-01 00:00:00
abstract：:Coronary blood flow depends upon the vascular resistance distributed non-uniformly within the coronary microcirculation. Coronary microvascular resistance is governed by metabolic, myogenic, endothelial and neurohumoral influences. A number of these control mechanisms interact locally within a microvascular segment. I...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-2946-0_17
更新日期：1993-01-01 00:00:00
