Latex-less opium poppy: cause for less latex and reduced peduncle strength.

Abstract:

:A genotype 'Sujata' developed earlier at CSIR-CIMAP from its parent 'Sampada' is considered to be the latex-less variety of Papaver somniferum. These two genotypes are contrasting in terms of latex and stem strength. Earlier we have carried out microarray analysis to identify differentially expressing genes from the capsules of the two genotypes. In this study, the peduncles of the two genotypes were compared for the anatomy revealing less number of laticifers in the cortex and vascular bundles. One of the important cell wall-related genes (for laccase) from the microarray analysis showing significantly higher expression in 'Sampada' capsule was taken up for further characterization in the peduncle here. It was functionally characterized through transient overexpression and RNAi suppression in 'Sujata' and 'Sampada'. The increase in acid insoluble lignin and total lignin in overexpressed tissue of 'Sujata', and comparable decrease in suppressed tissue of 'Sampada', along with corresponding increase and decrease in the transcript abundance of laccase confirm the involvement of laccase in lignin biosynthesis. Negligible transcript in phloem compared to the xylem tissue localized its expression in xylem tissue. This demonstrates the involvement of P. somniferum laccase in lignin biosynthesis of xylem, providing strength to the peduncle/stem and preventing lodging.

journal_name

Physiol Plant

journal_title

Physiologia plantarum

authors

Chaturvedi N,Singh SK,Shukla AK,Lal RK,Gupta MM,Dwivedi UN,Shasany AK

doi

10.1111/ppl.12086

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

436-45

issue

3

eissn

0031-9317

issn

1399-3054

journal_volume

150

pub_type

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