An apple NAC transcription factor enhances salt stress tolerance by modulating the ethylene response.

Abstract:

:It is known that ethylene signaling is involved in the regulation of the salt stress response. However, the molecular mechanism of ethylene-regulated salt stress tolerance remains largely unclear. In this study, an apple NAM ATAF CUC transcription factor, MdNAC047, was isolated and functionally characterized to be involved in ethylene-modulated salt tolerance. MdNAC047 gene was significantly induced by salt treatment and its overexpression conferred increased tolerance to salt stress and facilitated the release of ethylene. Quantitative real-time-PCR analysis demonstrated that overexpression of MdNAC047 increased the expression of ethylene-responsive genes. Electrophoretic mobility shift assay, yeast one-hybrid and dual-luciferase assays suggested that MdNAC047 directly binds to the MdERF3 (ETHYLENE RESPONSE FACTOR) promoter and activates its transcription. In addition, genetic analysis assays indicated that MdNAC047 regulates ethylene production at least partially in an MdERF3-dependent pathway. Overall, we found a novel 'MdNAC047-MdERF3-ethylene-salt tolerance' regulatory pathway, which provide new insight into the link between ethylene and salt stress.

journal_name

Physiol Plant

journal_title

Physiologia plantarum

authors

An JP,Yao JF,Xu RR,You CX,Wang XF,Hao YJ

doi

10.1111/ppl.12724

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

279-289

issue

3

eissn

0031-9317

issn

1399-3054

journal_volume

164

pub_type

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