Abstract:
:The role of APX (ascorbate peroxidase) in protection against oxidative stress was examined using transgenic tobacco plants. The full length cDNA, coding Arabidopsis thaliana L. APX fused downstream to the chloroplast transit sequence from A. thaliana glutathione reductase, was cloned into appropriate binary vector and mobilized into Agrobacterium tumefaciens C58C2. Leaf discs were infected with the Agrobacterium and cultured on medium supplied with kanamycin. The incorporation of the gene in tobacco genome was confirmed by Southern dot blot hybridization. Transgenic lines were generated, and the line Chl-APX5 shown to have 3.8-fold the level of APX activity in the wild-type plants. The isolated chloroplasts from this line showed higher APX activity. During early investigation, this line showed enhanced tolerance to the active oxygen-generating paraquat and sodium sulphite. The first generation of this line, also, showed enhanced tolerance to salt, PEG and water stresses, as determined by net photosynthesis. The present data indicate that overproducing the cytosolic APX in tobacco chloroplasts reduces the toxicity of H(2)O(2).
journal_name
Physiol Plantjournal_title
Physiologia plantarumauthors
Badawi GH,Kawano N,Yamauchi Y,Shimada E,Sasaki R,Kubo A,Tanaka Kdoi
10.1111/j.0031-9317.2004.00308.xsubject
Has Abstractpub_date
2004-06-01 00:00:00pages
231-238issue
2eissn
0031-9317issn
1399-3054pii
PPL308journal_volume
121pub_type
杂志文章abstract::alpha-Mannosidase (EC 3.2.1.24) from rice dry seeds was purified to homogeneity. Optimum pH and Km for pNP-alpha-Man hydrolysis were pH 4.3-4.5 and 1.04 mM, respectively. The enzyme digested mannobioses such as Manalpha-1,2Man, Manalpha-1,6Man, Manalpha-1,3Man but Manalpha-1,4Man. Zn2+ ion was required for the activit...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2001.1120103.x
更新日期:2001-05-01 00:00:00
abstract::Cuttings of Populus kangdingensis and Populus cathayana originating from altitudes of 3500 and 1500 m in southwestern China, respectively, were grown for one growing season in the field under ambient or ambient plus supplemental ultraviolet-B (UV-B) radiation with two levels of nutrients. In both species, enhanced UV-...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2009.01328.x
更新日期:2010-03-01 00:00:00
abstract::Cadmium (Cd2+) soil pollution is a global environmental problem caused by the high toxicity of Cd. 24-Epibrassinolide (EBR) is a biodegradable plant steroid involved in response modulation to biotic and abiotic stresses. The aim of this study was to evaluate if the leaf-application of EBR improves the gas exchange and...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.13182
更新日期:2020-08-06 00:00:00
abstract::The evolution of terrestrial plant life was made possible by the establishment of a root system, which enabled plants to migrate from aquatic to terrestrial habitats. During evolution, root organization has gradually progressed from a very simple to a highly hierarchical architecture. Roots are initiated during embryo...
journal_title:Physiologia plantarum
pub_type: 杂志文章,评审
doi:10.1111/ppl.12783
更新日期:2019-01-01 00:00:00
abstract::Pollen viability and germination are known to be sensitive to high temperature (HT). However, the mode by which high temperature impairs pollen functioning is not yet clear. In the present study, we investigated the effect of high temperature on changes occurring in carbohydrate of bell pepper (Capsicum annuum L. cv. ...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2001.1120407.x
更新日期:2001-08-01 00:00:00
abstract::A soluble anionic isoperoxidase (EC 1,11,1,7) was purified from peach (Prunus persica L. Batsch cv. Merry) seeds. Purification was achieved by DEAE-Sephacel, Sephacryl S-300 and CM-cellulose chromatography. The purified isoperoxidase de-carboxylated indole-3-acetic acid (S(0.5) 0.13 mM, Hill coefficient 1.7). Molecula...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.1990.tb00035.x
更新日期:1990-08-01 00:00:00
abstract::Regional climate change in Antarctica would favor the carbon assimilation of Antarctic vascular plants, since rising temperatures are approaching their photosynthetic optimum (10-19°C). This could be detrimental for photoprotection mechanisms, mainly those associated with thermal dissipation, making plants more suscep...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12739
更新日期:2019-03-01 00:00:00
abstract::The effect of various concentrations of aminoethoxyvinylglycine (AVG; 0.32 and 1.28 mM), an ethylene biosynthesis inhibitor, and of the polyamines putrescine (10 mM), spermidine (0.1, 1 and 5 mM) and spermine (2 mM) on peach (Prunus persica L. Batsch cv. Redhaven) fruit ripening was evaluated under field conditions. T...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2002.1140317.x
更新日期:2002-03-01 00:00:00
abstract::Photosystem II (PSII) activity was examsined in leaves of chilling-sensitive cucumber (Cucumis sativus L.), tomato (Lycopersicum esculentum L.), and maize (Zea mays L.), and in chilling-tolerant barley (Hordeum vulgare L.) illuminated with moderate white light (300 micro mol m(-2) s(-1)) at 4 degrees C using chlorophy...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.0031-9317.2004.00305.x
更新日期:2004-06-01 00:00:00
abstract::Endophytic fungi are potential sources of secondary metabolites; however, they are little known for phytohormones secretion and amelioration of plant growth under abiotic stresses. We isolated a novel endophyte from the roots of Cucumis sativus and identified it as a strain of Exophiala sp. by sequencing internal tran...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2011.01508.x
更新日期:2011-12-01 00:00:00
abstract::The massive increase in carbon dioxide concentration in the atmosphere driven by human activities is causing huge negative consequences and new sustainable sources of energy, food and materials are highly needed. Algae are unicellular photosynthetic microorganisms that can provide a highly strategic contribution to th...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12915
更新日期:2019-05-01 00:00:00
abstract::The effect of variable autumn temperatures in combination with decreasing irradiance and daylength on photosynthesis, growth cessation and freezing tolerance was investigated in northern- and southern-adapted populations of perennial ryegrass (Lolium perenne) and timothy (Phleum pratense) intended for use in regions a...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12548
更新日期:2017-07-01 00:00:00
abstract::Medicago intertexta and Melilotus indicus, two wild leguminous herbs with different tolerance to salinity were investigated for NaCl-induced changes in the expression level of some Na(+) transporters. M. indicus plants grew well at NaCl concentration from 0 to 400 mM, whereas growth of M. intertexta plants was severel...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2007.00940.x
更新日期:2007-09-01 00:00:00
abstract::Raphanus sativus L. cv. Rimbo was grown for 10 days after emergence in hydroponic culture containing 0.12 (control), 5, 10 and 15 micro M copper. The seeds were germinated in the presence of the copper solution. The Cu contents increased with the treatment in both shoots and roots, maintaining in the roots a value eig...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2003.00068.x
更新日期:2003-05-01 00:00:00
abstract::We investigated the fate of carbon dioxide (CO2 ) absorbed by roots or internally produced by respiration using gas exchange and stable isotopic labeling. CO2 efflux from detached leaves supplied with bicarbonate/CO2 solutions was followed over six cycles. CO2 effluxes were detected when bicarbonate solution at high p...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12767
更新日期:2019-03-01 00:00:00
abstract::The small monocot plant Brachypodium distachyon is rapidly emerging as a powerful model system to study questions unique to the monocot crops. An extensive BLAST search was carried to identify putative orthologues of the Arabidopsis NRT2 genes in the fully sequenced Brachypodium genome. Seven genes encoding putative h...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12716
更新日期:2019-03-01 00:00:00
abstract::Cucumber (Cucumis sativus L.) cv. Xintaimici (a chilling-resistant cultivar) and cv. Jinyan no. 4 (a chilling-sensitive cultivar) were subjected to two temperatures (15/15 and 25/18 degrees C) under low light (100 mumol m(-2) s(-1)) to understand the relationship between ultrastructural changes and the antioxidant abi...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2007.01036.x
更新日期:2008-04-01 00:00:00
abstract::Sequestration of Pb by synthetic chelates has been reported to increase bioavailability, uptake, and translocation of this metal in plants. In this work the potential phytotoxic effects of Pb-EDTA were investigated in Phaseolus vulgaris L. cv. Limburgse vroege plants grown on hydroponics. Addition of 50 microM Pb-EDTA...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2002.1150307.x
更新日期:2002-07-01 00:00:00
abstract::Shoot-root communication is involved in plant stress responses, but its mechanism is largely unknown. To determine the role of roots in stress tolerance, cucumber (Cucumis sativus) shoots from plants with roots of their own or with figleaf gourd (Cucurbita ficifolia, a chilling-tolerant species) or luffa (Luffa cylind...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12200
更新日期:2014-11-01 00:00:00
abstract::A treatment period as brief as 8 h in 10(-3) M EGTA completely blocks gravitropism in 70-80% of the treated coleoptiles of oats (Avena sativa L. cv. Garry) without inhibiting growth. Only about 10% of the plants perfused in water failed to exhibit gravitropism. Subsequent perfusion of EGTA-treated plants with calcium ...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.1984.tb06354.x
更新日期:1984-07-01 00:00:00
abstract::Inositol polyphosphate kinases play important roles in diverse cellular processes. In this study, the function of an inositol polyphosphate kinase gene homolog named ThIPK2 from a dicotyledonous halophyte Thellungiella halophila was investigated. The deduced translation product (ThIPK2) shares 85% identity with the Ar...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2009.01235.x
更新日期:2009-08-01 00:00:00
abstract::Methyl jasmonate (MeJA) is an airborne signaling phytohormone that can induce changes in endogenous jasmonates (JAs) and cause photosynthetic responses. However, the response of these two aspects of citrus plants at different MeJA concentrations is still unclear. Four MeJA concentrations were used in two citrus variet...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.13170
更新日期:2020-11-01 00:00:00
abstract::Time courses of photochemical reflectance index (PRI) of an attached cucumber leaf during a dark-light transition were compared with those of photochemical yields of photosystem II (YII ) to discuss the feasibility of PRI imaging for estimating the efficiency of photosynthetic light use. YII and PRI were simultaneousl...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12745
更新日期:2019-03-01 00:00:00
abstract::Sweet sorghum (Sorghum bicolor) is a C4 drought resistant species with a huge potential for bioenergy. Accentuated reductions in water availability for crop production and altered rainfall distribution patterns, however, will have direct impact on its physiological attributes, metabolic functions and plant growth. The...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12016
更新日期:2013-09-01 00:00:00
abstract::In plants, CALCIUM-DEPENDENT PROTEIN KINASES (CDPKs/CPKs) are involved in calcium signaling in response to endogenous and environmental stimuli. Here, we report that ZmCPK11, one of maize CDPKs, participates in salt stress response and tolerance. Salt stress induced expression and upregulated the activity of ZmCPK11 i...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12938
更新日期:2020-01-01 00:00:00
abstract::The ATP-binding cassette (ABC) superfamily is a large protein family with diverse physiological functions in all kingdoms of life. One distinguished subfamily, the pleiotropic drug resistance (PDR) transporters, has only been identified in plants and fungi. Here, we identified a Nicotiana tabacum PDR gene, NtPDR6, whi...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/ppl.12267
更新日期:2015-02-01 00:00:00
abstract::The suitability of the infiltration-centrifugation method for collection of apoplastic fluid from intact leaves was evaluated for different plant species. Large differences with respect to infiltrability of the leaves, which correlated inversely with stomatal and mesophyll resistance, became apparent. Osmolality of in...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1034/j.1399-3054.2001.1110405.x
更新日期:2001-04-01 00:00:00
abstract::Flowering and dwarfism induced by 5-azacytidine and zebularine, which both cause DNA demethylation, were studied in a short-day (SD) plant Pharbitis nil (synonym Ipomoea nil), var. Violet whose photoinduced flowering state does not last for a long period of time. The DNA demethylating reagents induced flowering under ...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2010.01345.x
更新日期:2010-05-01 00:00:00
abstract::To understand the role of fruit cuticle lipid composition in fruit water loss, an advanced backcross population, the BC(2)F(2) , was created between the Capsicum annuum (PI1154) and the Capsicum chinense (USDA162), which have high and low post-harvest water loss rates, respectively. Besides dramatic differences in fru...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.2012.01592.x
更新日期:2012-09-01 00:00:00
abstract::Using Avena sativa L. cv. Victory oat seedlings and excised p-1 stem segments (including the p-1 and p-2 internodes) the effect of exogenously supplied ethylene and the removal of ethylene on internodal extension and gravitropic bending was assessed. Similarly, the ability of the excised system to respond to gravisti...
journal_title:Physiologia plantarum
pub_type: 杂志文章
doi:10.1111/j.1399-3054.1985.tb02389.x
更新日期:1985-01-01 00:00:00