Purification of an anionic isoperoxidase from peach seeds and its immunological comparison with other anionic isoperoxidases.

Abstract:

:A soluble anionic isoperoxidase (EC 1,11,1,7) was purified from peach (Prunus persica L. Batsch cv. Merry) seeds. Purification was achieved by DEAE-Sephacel, Sephacryl S-300 and CM-cellulose chromatography. The purified isoperoxidase de-carboxylated indole-3-acetic acid (S(0.5) 0.13 mM, Hill coefficient 1.7). Molecular mass, determined by gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis, was ca 60 kDa. Polyclonal antibodies were raised in rabbit against this isoperoxidase. Using immunoprecipitation this isoenzyme was found to be immunologically different from other soluble anionic isoperoxidases isolated from peach seeds.

journal_name

Physiol Plant

journal_title

Physiologia plantarum

authors

Quesada MA,Tigier HA,Bukovac MJ,Valpuesta V

doi

10.1111/j.1399-3054.1990.tb00035.x

subject

Has Abstract

pub_date

1990-08-01 00:00:00

pages

623-8

issue

4

eissn

0031-9317

issn

1399-3054

journal_volume

79

pub_type

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