Abstract:
:We studied copper uptake in inside-out plasma membrane vesicles derived from roots of copper-sensitive, moderately copper-tolerant and highly copper-tolerant populations of Silene vulgaris (Amsterdam, Marsberg and Imsbach, respectively). Plasma membrane vesicles were isolated using the two-phase partitioning method and copper efflux was measured using direct filtration experiments. Vesicles derived from Imsbach plants accumulated two and three times more copper than those derived from Marsberg and Amsterdam plants, respectively. This accumulation was ATP-dependent. Also, 9-amino-6-chloro-2-methoxyacridine fluorescence quenching rates upon copper addition decreased in the order Imsbach>Marsberg>Amsterdam. Our results support the hypothesis that efflux of copper across the root plasma membrane plays a role in the copper tolerance mechanism in S. vulgaris.
journal_name
Physiol Plantjournal_title
Physiologia plantarumauthors
Van Hoof NA,Koevoets PL,Hakvoort HW,Ten Bookum WM,Schat H,Verkleij JA,Ernst WHdoi
10.1034/j.1399-3054.2001.1130210.xsubject
Has Abstractpub_date
2001-10-01 00:00:00pages
225-232issue
2eissn
0031-9317issn
1399-3054pii
1130210journal_volume
113pub_type
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