Enhanced ATP-dependent copper efflux across the root cell plasma membrane in copper-tolerant Silene vulgaris.

Abstract:

:We studied copper uptake in inside-out plasma membrane vesicles derived from roots of copper-sensitive, moderately copper-tolerant and highly copper-tolerant populations of Silene vulgaris (Amsterdam, Marsberg and Imsbach, respectively). Plasma membrane vesicles were isolated using the two-phase partitioning method and copper efflux was measured using direct filtration experiments. Vesicles derived from Imsbach plants accumulated two and three times more copper than those derived from Marsberg and Amsterdam plants, respectively. This accumulation was ATP-dependent. Also, 9-amino-6-chloro-2-methoxyacridine fluorescence quenching rates upon copper addition decreased in the order Imsbach>Marsberg>Amsterdam. Our results support the hypothesis that efflux of copper across the root plasma membrane plays a role in the copper tolerance mechanism in S. vulgaris.

journal_name

Physiol Plant

journal_title

Physiologia plantarum

authors

Van Hoof NA,Koevoets PL,Hakvoort HW,Ten Bookum WM,Schat H,Verkleij JA,Ernst WH

doi

10.1034/j.1399-3054.2001.1130210.x

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

225-232

issue

2

eissn

0031-9317

issn

1399-3054

pii

1130210

journal_volume

113

pub_type

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