Abstract:
OBJECTIVES:Cervical cancer is the most common malignant tumors in women leading to serious morbidity and mortality worldwide, especially among developing countries. A main cause of the disease is the high-risk human papillomavirus (HR-HPV) infection. HSIL usually progress to cervical cancer, and low-grade lesions, including LSIL and ASCUS, mostly turn to normal or benign lesions, but there are still a small number of patients who will progress to HSIL. Up to now there is no efficient biomarker clinically available to predict people with high risk to progress into HSIL. This study was conducted to evaluate the value of human papillomavirus (HPV) DNA, p16INK4a protein, and HPV L1 capsid protein in predicting HSIL and minimizing unnecessary colposcopy treatments. METHODS:1222 patients with HR-HPV infection or with abnormal Thinprep cytologic test (TCT) were chosen to conduct colposcopy in the cervical out-patient clinic of Shanghai First Maternity and Infant Hospital affiliated to Shanghai Tongji University from June 2014 to January 2017. TCT, cervical biopsy, HPV DNA and HPVL1 were performed on all patients. 110 patients were selected to detect p16INK4a protein. Hybrid capture 2 (HC-2) was used to detect HPV DNA, and their subgroups using gene typing system. Immunohistochemical technology was used to detect HPV L1 and p16. RESULTS:HPV DNA was positive in 1097 cases, with the positive rate of 89.7% (1097/1222). In particular, the positive expression rates of HPV DNA were 82.3, 95.7, 96.6 and 100% in Normal/CC, LSIL, HSIL and cervical cancer groups, respectively (p < 0.001). HPV L1 was negative in 781 cases with HR-HPV infection, and the overall negative rate is 71.1%. In patients with Normal/CC, LSIL and HSIL, the negative expression rates of HPV L1 were 91.3, 40 and 81.2%, respectively (p value < 0.001). In the 110 patients, HPV L1 was negative in 98.1% (53/54) of Normal/CC, 42.9% (12/28) of LSIL and 85.1% (23/27) of HSIL (p value = 0.0043). P16-positive rates in patients with Normal/CC, LSIL and HSIL were 33.3% (18/54), 75% (21/28) and 96.2% (26/27), respectively (p value < 0.001). 18 out of 28 cases express low positive (+) in LSIL, 25 out of 27 cases express strong positive (3+) in HSIL. Patients with L1(-) p16(+) including 18.5% (10/54) of normal/cervicitis, 60.7% (17/28) of LSIL and 85.1% (23/27) of HSIL (p value < 0.005). Furthermore, patients with L1(-) p16(1+) included 37% (10/27) of normal/cervicitis 59.3% (16/27) of LSIL and 3.7% (1/27) of HSIL; patients with L1(-) p16(2+) consisted of 0% of normal/cervicitis/LSIL and 100% (1/1) of HSIL; patients with L1(-) p16(3+) were composed of 0% of normal/cervicitis, 4.5% (1/22) of LSIL and 95.5% (21/22) of HSIL (p value < 0.005) (Table 6). CONCLUSION:With the increase in the degree of the cervical lesions, the expression of HPV DNA and p16 is up-regulated while HPV L1 protein is down-regulated. HPV DNA, HPV L1 and p16 are useful markers for the prediction of HSIL. Combined detection of these three markers has important potential to predicting HSIL and minimizing unnecessary colposcope examination.
journal_name
Arch Gynecol Obstetjournal_title
Archives of gynecology and obstetricsauthors
Hu H,Zhao J,Yu W,Zhao J,Wang Z,Jin L,Yu Y,Han L,Wang L,Zhu H,Li Fdoi
10.1007/s00404-018-4931-1subject
Has Abstractpub_date
2019-01-01 00:00:00pages
141-149issue
1eissn
0932-0067issn
1432-0711pii
10.1007/s00404-018-4931-1journal_volume
299pub_type
杂志文章abstract:OBJECTIVES:To compare the diagnostic accuracy of brush endometrial sampling with conventional dilatation and curettage in women with abnormal uterine bleeding. PATIENTS AND METHODS:Two hundred and twenty (220) women with abnormal uterine bleeding were included in this comparative study; endometrial sampling was done b...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-014-3523-y
更新日期:2015-05-01 00:00:00
abstract::A kit using mouse monoclonal antibody against human alpha-fetoprotein (anti-AFP kit) has been developed. Cervical secretion (n = 34) and vaginal fluid (n = 15) was obtained from 30 pregnant volunteers. All 31 specimens obtained from women with intact membranes tested negative while all 18 specimens from women with pre...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/BF00634709
更新日期:1995-01-01 00:00:00
abstract:PURPOSE:To test the reliability of the admission test to identify the compromised fetus and thus reduce the neonatal morbidity and mortality by early intervention. METHODS:A prospective analysis over a period of 1 year from December 2007 to December 2008 included 100 antepartum patients and were evaluated for perinata...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-010-1406-4
更新日期:2010-10-01 00:00:00
abstract:PURPOSE:Color Doppler ultrasound (CDU) accuracy of kidneys and bladder in the diagnosis and treatment of hydronephrosis and/or renal colic in pregnancies was evaluated. METHODS:Between January 2010 and September 2012, 234 pregnant women asymptomatic in 204 cases and with unilateral renal colic in 30 (median 26 years) ...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-013-2768-1
更新日期:2013-09-01 00:00:00
abstract::Early detection and repair of bladder perforation reduce postoperative morbidity. We describe two cases of bladder perforation sustained during complicated laparoscopic adhesiolysis and discuss a previously reported simple method of detecting bladder trauma during operative laparoscopy. ...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-007-0413-6
更新日期:2008-01-01 00:00:00
abstract::Tubal sterilization is one of the options chosen by the women who have completed their fertility. Although ectopic pregnancy after tubal sterilization is rare, all women undergoing this procedure should be informed about the risk of it. An ectopic pregnancy in a 36-year-old woman who had undergone tubal sterilization ...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/pl00007502
更新日期:2002-01-01 00:00:00
abstract::Malignant transformation of mature cystic teratomas is uncommon but present in clinical practice. Especially in postmenopausal women, the clinical manifestation of a mature teratoma with undiagnosed malignant transformation as acute abdomen is extremely rare. In these cases, total hysterectomy with bilateral salpingo-...
journal_title:Archives of gynecology and obstetrics
pub_type: 信件
doi:10.1007/s00404-009-1097-x
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:hTFM in primary vulvar cancer is an important prognostic factor. Ideally, a diameter of > 8 mm should be achieved after primary surgery. The role of VIN III persistence after primary surgery in vulvar cancer is still unclear. The main objective of the current study was to study the role of residual VIN III r...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-018-4887-1
更新日期:2018-11-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to compare a low-dose aspirin treatment on placental and perinatal effects in the patients with poor obstetric history such as preeclampsia, intrauterine growth retardation (IUGR) in previous pregnancy. STUDY DESIGN:This retrospective study of 86 pregnant women was conducted between...
journal_title:Archives of gynecology and obstetrics
pub_type: 临床试验,杂志文章
doi:10.1007/s00404-006-0162-y
更新日期:2006-07-01 00:00:00
abstract:PURPOSE:To evaluate the efficacy and safety for mother and child of using intravenous iron isomaltoside (IV-IIM) during pregnancy. METHODS:Using an appointment register, we retrospectively identified all pregnant women who received a single dose of 1000 or 1500 mg IV-IIM in the maternity ward of Falu Hospital and subs...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章,评审
doi:10.1007/s00404-020-05509-2
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:The Nitric Oxide (NO) system plays an important role in the establishment and maintenance of the feto-placental circulation. Research on the pathogenesis of preeclampsia in several studies has established the involvement of the NO-system in preeclampsia and fetal intrauterine growth restriction (IUGR). In th...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-006-0149-8
更新日期:2006-06-01 00:00:00
abstract:PURPOSE:To analyze the effect of Chlamydia trachomatis (C. trachomatis) on adverse pregnancy outcomes based on the currently available evidence. METHODS:Multiple databases were comprehensively searched from the available date of inception through December 9, 2019. The effect of C. trachomatis on adverse pregnancy outc...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00404-020-05664-6
更新日期:2020-09-01 00:00:00
abstract:PURPOSE:Both cigarette smoking and alcohol consumption are somehow implicated in sperm function, but the impact of these two lifestyle factors on sperm parameters remains controversial. The present study is focused on the impact of cigarette smoking and alcohol consumption separately and combined on sperm parameters an...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-014-3281-x
更新日期:2014-10-01 00:00:00
abstract:OBJECTIVE:Our objective was to review the hysterectomies performed in our clinic for the last 17 years to reveal the facts in relation to the operation. MATERIALS AND METHODS:The records of the patients who had undergone hysterectomy in Istanbul University Cerrahpasa School of Medicine Department of Obstetrics and Gyn...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-003-0546-1
更新日期:2004-12-01 00:00:00
abstract:PURPOSE:To identify risk factors for the development of severe perineal lacerations and to give recommendations for their prevention in nulliparous women. METHODS:A retrospective case-control analysis of deliveries at our University Hospital was performed. Multiparae, Caesarean sections, twin pregnancies, fetal breech...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-009-1063-7
更新日期:2010-01-01 00:00:00
abstract::Vaginal hysterectomy is less invasive than hysterectomy performed via the abdominal approach. The vaginal approach may be made difficult by prior conisation, a need to remove the adnexae or marked uterine enlargement requiring morcellement. In this retrospective study we have investigated the impact of these factors o...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/BF02390389
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:The aim of this study was to evaluate the phosphorylated isoform of insulin-like growth factor binding protein-1 (phIGFBP-1) in endocervical secretions as a predictor of preterm delivery in symptomatic and asymptomatic pregnant women. METHODS:The study included 105 patients between 24 and 34 weeks' gestatio...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-008-0703-7
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:This study aimed to confirm that biglycan (BGN) can promote the migration and invasion in endometrial cancer both in vitro and in vivo and the possible therapeutic value of BGN in endometrial cancer. METHODS:Western blot was used to screen out the higher protein level of BGN in human endometrial cancer cells...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-015-3844-5
更新日期:2016-02-01 00:00:00
abstract:PURPOSE:To identify key genes related to the effect of estrogen on ovarian cancer. METHODS:Microarray data (GSE22600) were downloaded from Gene Expression Omnibus. Eight estrogen and seven placebo treatment samples were obtained using a 2 × 2 factorial designs, which contained 2 cell lines (PEO4 and 2008) and 2 treatm...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-015-3833-8
更新日期:2016-02-01 00:00:00
abstract:PURPOSE:To calculate the measurement error of the hourly fetal urine production rate (HFUPR) and evaluate the implication of different methods for measuring the HFUPR, i.e. ellipsoid versus sum-of-cylinders method. METHODS:The calculation was based on sonographic documentation of the increased bladder volumes during t...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-009-1242-6
更新日期:2010-11-01 00:00:00
abstract:PURPOSE:To evaluate intracytoplasmic sperm injection (ICSI) outcome of women over age 39 and to determine when to discourage such couples to undergo IVF using their own oocytes. METHODS:Four hundred ninety-five consecutive women (n=668 cycles) over age 39 were evaluated by year-by-year age increments to discriminate t...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-009-1116-y
更新日期:2010-02-01 00:00:00
abstract:PURPOSE:Τo estimate the discrepancy rate between colposcopy, punch biopsy histology and surgical specimen histology as well as the positive (PPV) and negative predictive value (NPV) of colposcopic diagnosis for high-grade squamous intraepithelial lesions (HGSIL). METHODS:A prospective study was conducted during the pe...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-018-4714-8
更新日期:2018-05-01 00:00:00
abstract:PURPOSE:Many studies have demonstrated the effectiveness of isoflavones on menopausal symptoms; however, these mostly used high dosages. Because high-dose isoflavone may result in endometrial hyperplasia, we investigated whether low-dose isoflavone aglycone alleviates menopausal symptoms similarly to high dosages. MET...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00404-015-3849-0
更新日期:2016-03-01 00:00:00
abstract::Uterine leiomyomas affect 20-30 % of women 35 years and older. Extrauterine leiomyomas are rare and present a greater diagnostic challenge. Those unusual growth patterns occur more often in women of reproductive age with a history of hysterectomy or surgery for uterine leiomyomas. They have been reported in the litera...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章,评审
doi:10.1007/s00404-014-3356-8
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:To evaluate the clinical outcomes after fresh transfer of blastocysts cultured from vitrified-thawed cleavage embryos (VTCE) compared with conventional frozen-thawed blastocysts transfer (FBT), or with the usual fresh blastocysts transfer (FRBT). METHODS:A total of 155 cycles undergoing fresh transfer of VTCE ...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-019-05236-3
更新日期:2019-09-01 00:00:00
abstract:BACKGROUND:MicroRNAs (miRNAs) have been increasingly exploited in human malignancies. The regulation of microRNA-455-5p (miR-455-5p) has been shown in several cancers, except for cervical carcinoma. Therefore, the role of miR-455-5p was exploited in cervical carcinoma. METHODS:The qRT-PCR experiment was used to assess...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-020-05536-z
更新日期:2020-05-01 00:00:00
abstract:PURPOSE:To investigate the relationship between hyaluronan binding (HB) assay and pregnancy rates in intrauterine insemination (IUI) cycles. METHODS:This prospective cohort study was done in Hacettepe University, a tertiary care center for reproductive medicine. Seventy-one consecutive couples who suffered from unexpl...
journal_title:Archives of gynecology and obstetrics
pub_type: 临床试验,杂志文章
doi:10.1007/s00404-012-2476-2
更新日期:2012-12-01 00:00:00
abstract:PURPOSE:The occurrence of diminished ovarian reserve (DOR) in women was growing in recent years. Although in vitro fertilization and embryo transfer (IVF-ET) became an effective treatment for DOR, the live-birth (LB) rate remains unsatisfactory. This study aimed to investigate the impact factors of LB rate in women wit...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/s00404-018-4884-4
更新日期:2018-11-01 00:00:00
abstract::We report the case of a 66-year-old woman who had a hysterectomy because of uterine tumour. Histological examination showed a leiomyoma with degenerative changes and extensive extramedullary haematopoiesis. There was no evidence of any haematological or systemic disease. Extramedullary haematopoiesis in patients witho...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/BF02389579
更新日期:1990-01-01 00:00:00
abstract::Between 1969 and 1991 there were 166,410 births in Southern Israel with 13 maternal deaths (7.8/100,000). In the Jewish population there were 119,130 deliveries with 7 maternal deaths (5.9/100,000), and the Bedouins had 47,280 deliveries with 6 maternal deaths (12.7/100,000). Prenatal care was an important preventive ...
journal_title:Archives of gynecology and obstetrics
pub_type: 杂志文章
doi:10.1007/BF02426359
更新日期:1993-01-01 00:00:00