Abstract：
:Soluble proteins from leaves of Nicotiana glauca Grah., N. langsdorffii Weinm., their reciprocal hybrids and amphiploid hybrid (N. glaucaxN. langsdorffii) were resolved by two-dimensional gel electrophoresis. Among a group of well-resolved polypeptides, in the isoelectric-point range of 5-5.5 and relative-molecular-mass (Mr) range of 18-23 kilodaltons (kDa), species-specific variation was observed. Polypeptides designated "L" and "l" are specific to N. langsdorffii, and "G" and "g" to N. glauca, while "C" is common to both species. Polypeptides "L", "G" and "C" are localized in the chloroplasts and associated with thylakoid membranes. Polypeptide "L" is more acidic than polypeptide "G", and both polypeptides have an Mr of 23 kDa. They were isolated from two-dimensional gels and their first 13 N-terminal amino-acid sequences were determined. These were found to be identical to the 13N-terminal amino acids of the photosystem II (PSII) 23-kDa polypeptide from spinach (T. Jansen et al. (1987) FEBS Lett. 216, 234-240) and, except for one change, to those from pea (R. Wales et al. (1989) Plant Molec. Biol., in press). Polypeptides "G" and "L" cross-react with antiserum against the PSII 23-kDa polypeptide from pea. Therefore, polypeptides "G" and "L" are extrinsic PSII 23-kDa polypeptides. They appear jointly and in equal amounts in the reciprocal hybrids. Since chloroplasts in Nicotiana are maternally inherited, these results demonstrate that polypeptides "G" and "L" are encoded by nuclear genes, are polymorphic variants of the PSII 23-kDa polypeptide, and are inherited in a Mendelian manner.
journal_name
Plantajournal_title
Plantaauthors
Hua SB,Dube SK,Barnett NM,Kung SDdoi
10.1007/BF00391086subject
Has Abstractpub_date
1989-10-01 00:00:00pages
397-402issue
3eissn
0032-0935issn
1432-2048journal_volume
179pub_type
杂志文章相关文献
PLANTA文献大全abstract：:Transitory starch is stored during the day inside chloroplasts and then broken down at night for export. Recent data indicate that maltose is the major form of carbon exported from the chloroplast at night but its fate in the cytosol is unknown. An amylomaltase gene ( malQ) cloned from Escherichia coli is necessary fo...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-003-1127-z
更新日期：2004-01-01 00:00:00
abstract：:Studies reported here reveal a low nitrate reductase activity in the shoots of MTU 9 rice plants while in roots high enzyme activity has been recorded.Under pathogenesis, a low nitrate reductase activity in the roots and a high enzyme activity in the shoots have been recorded in susceptible rice plants. In the resista...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00390132
更新日期：1966-06-01 00:00:00
abstract：:The induction of monospore-development in the Conchocelis stage of Porphyra tenera KJELLM. was investigated. 1. Experiments with light-breaks during the dark-periods indicate the decisive role of a photoperiodic control. 2. The critical daylength is approximately 10 hr. Daily light periods longer than 10 hrs inhibit t...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00387423
更新日期：1967-03-01 00:00:00
abstract：:A 5-methyltryptophan(5-MT)-resistant cell line of wild carrot (Daucus carota L.), W001, that exhibited auxin-independent callus growth, was found to accumulate indole-3-acetic acid (IAA) and tryptophan (trp). Anthranilate-synthetase activity in W001 cell extract was less sensitive to feedback inhibition by trp than in...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388358
更新日期：1979-01-01 00:00:00
abstract：:Phaseolus coccineus embryos at the heartshaped and the middle cotyledonary stages were cultured in vitro on media added with different concentrations of zeatin (Z) or zeatin riboside (Zr). Growth of early embryos was clearly favored by concentrations of Z from 10(-8) M to 10(-5) M, lower concentrations having no effec...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00384586
更新日期：1979-10-01 00:00:00
abstract：:In this work, we analyze protein phosphatase (PP) involvement in the sucrose-mediated induction of fructan metabolism in wheat (Triticum aestivum). The addition of okadaic acid (OA), a PP-inhibitor, to sucrose-fed leaves reduced fructosylsucrose-synthesizing activity (FSS) induction in a dose-dependent manner. The exp...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-009-1002-7
更新日期：2009-10-01 00:00:00
abstract：:The ability of wild potatoes (Solanum spp.) to adapt to potentially stressful environmental temperatures was investigated by measuring the cold and heat tolerances of plants grown near sea-level in Lima following collection of tubers from plants growing naturally at altitudes ranging from 450 to 4,200 m. Relative cold...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00392980
更新日期：1983-10-01 00:00:00
abstract：:Soybeans provide an excellent source of protein in animal feed. Soybean protein quality can be enhanced by increasing the concentration of sulfur-containing amino acids. Previous attempts to increase the concentration of sulfur-containing amino acids through the expression of heterologous proteins have met with limite...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-011-1487-8
更新日期：2012-01-01 00:00:00
abstract：:Cyanelles isolated from the alga Cyanophora paradoxa Korschikoff synthesized cyanelle proteins in vitro. This synthesis was stimulated by light and totally inhibited by chloramphenicol. Cycloheximide had only a small inhibitory effect. Electrophoretic separation of the labelled soluble cyanelle proteins yielded at lea...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00394488
更新日期：1988-01-01 00:00:00
abstract：:Transpiration from excised leaves of Anthephra pubescens Nees was enhanced by 1 and 10 mmol m(-3) kinetin. Stomatal opening in isolated epidermal strips of A. pubescens under CO2-free air and in the absence of K(+) was enhanced by 10 mmol m(-3) kinetin. ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388157
更新日期：1977-01-01 00:00:00
abstract：:In the course of the life cycle of synchronized Euglena gracilis (14: 10 h light-dark cycles) two separate peaks of plastidial DNA synthesis appear. A first maximum (stage I) occurs 1 to 2 hours, after initiation of the light period, the second maximum (stage II) 9 to 10 hours after beginning of the light period. The ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00390074
更新日期：1975-01-01 00:00:00
abstract：:We used a heterologous system (transgenic Nicotiana tabacum L.) to investigate the processing, assembly and targeting of phytohemagglutinin (PHA), the lectin of the common bean, Phaseolus vulgaris L. In the bean, this glycoprotein accumulates in the protein bodies of the storage parenchyma cells in the cotyledons, and...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00392425
更新日期：1988-08-01 00:00:00
abstract：:The differentiation of phloem anastomoses linking the longitudinal vascular bundles has been studied in stem internodes of Cucurbita maxima Duchesne, C. pepo L. and Dahlia pinnata Cav. These anastomoses comprise naturally occurring regenerative sieve tubes which redifferentiate from interfascicular parenchyma cells in...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00262647
更新日期：1996-04-01 00:00:00
abstract：:The presence of a newly formed primary cell wall was shown to be required for attachment and subsequent transformation of tobacco leaf protoplasts by Agrobacterium tumefaciens in cocultivation experiments. In these experiments both protoplasts at different stages after their isolation and cell-wall inhibitors were use...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00401165
更新日期：1985-11-01 00:00:00
abstract：MAIN CONCLUSION:We introduced a novel combination of chromatographic techniques for the purification and analysis of a new UV-sunscreen mycosporine-like amino acid (MAA) in the terrestrial green alga Prasiola calophylla. Prasiola calophylla (Carmichael ex Greville) Kützing (Trebouxiophyceae, Chlorophyta) is a typical m...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-015-2396-z
更新日期：2016-01-01 00:00:00
abstract：:Experiments have been carried out to determine the basis for the dependence of chloroplast pigment synthesis on protein synthesis in dark-grown cells of Euglena gracilis greening in the light. The complete inhibition of chlorophyll synthesis brought about by actidione (10 μg/ml) when added half way through the greenin...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00406651
更新日期：1967-06-01 00:00:00
abstract：:The regulation of gravistimulation-induced ethylene production and its role in gravitropic bending was studied in Antirrhinum majus L. cut flower stems. Gravistimulation increased ethylene production in both lower and upper halves of the stems with much higher levels observed in the lower half. Expression patterns of ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-004-1359-6
更新日期：2005-01-01 00:00:00
abstract：:A major wall protein of suspension-cultured cells of French bean has been isolated and characterised. It can be prepared from walls or the culture filtrate and in composition it is particularly rich in proline, valine and glutamic acid/glutamine and contains appreciable amounts of hydroxyproline. The N-terminus shows ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00201935
更新日期：1992-05-01 00:00:00
abstract：MAIN CONCLUSION:Four polygalacturonase gene family members were highlighted that contribute to elucidate the roles of polygalacturonase during the fertility conversion process in male-sterile wheat. Polygalacturonase (PG) belongs to a large family of hydrolases with important functions in cell separation during plant g...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-020-03435-w
更新日期：2020-08-01 00:00:00
abstract：:The occurrence and distribution of a multifunctional chaperonin-60 (cpn60), the GroEL protein, was demonstrated in the cyanobacterium Anabaena PCC 7120 by using a rabbit anti-GroEL (Escherichia coli) antibody. Western-blot analysis showed a distinct cross-reaction with a protein of approx. 65 kilodaltons, analogous to...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00197575
更新日期：1991-12-01 00:00:00
abstract：:Gravitropic plant growth is due to gravistimulated asymmetric extension rates of the affected flanks of the graviresponding organ. Differential growth of the upper and lower flanks (UFs, LFs) of graviresponding plant organs may in principle be achieved by various biochemical and/or biophysical asymmetries. The gravist...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/pl00008100
更新日期：1997-09-01 00:00:00
abstract：:A method has been devised to ensure capture of large numbers of live paramecia within a short period of time under controlled conditions by the bladders of Utricularia gibba. The method permits a direct evaluation of the role of entrapped animals in the nutrition of this carnivorous plant. Paramecia captured by the bl...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385021
更新日期：1968-06-01 00:00:00
abstract：:Following tuber induction, potato tubers undergo a period of dormancy during which visible bud growth is inhibited. The length of the dormancy period is under environmental, physiological and hormonal control. Sucrose availability is one prerequisite for bud break. In the absence of sucrose, no bud break occurs. Thus,...
journal_title：Planta
pub_type： 杂志文章,评审
doi：10.1007/s00425-013-1968-z
更新日期：2014-01-01 00:00:00
abstract：:Isolated chloroplasts from spinach leaf cells, chloroplast subfractions, and a cell-free system of the cyanobacterium Synechococcus CCAP 6312 incorporated [1-(14)C]isopentenyl pyrophosphate in high yields into prenyl lipids. Products were polyprenols (C20, C45) chlorophylls, quinoid compounds, and fatty acid prenyl es...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00395899
更新日期：1985-01-01 00:00:00
abstract：:Etiolated maize leaves (Zea mays L.) contain a major isozyme of NADP-dependent malic enzyme (L-malate dehydrogenase, decarboxylating, EC 1.1.1.40) having an isoelectric point of 5.28±0.03, a Km (L-malate) 0.3-0.6 mM at pH 7.45; a broad pH optimum around pH 6.9 under the conditions of assay; a molecular weight of 280,0...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00388771
更新日期：1979-01-01 00:00:00
abstract：:Free porphyrins and their magnesium complexes, including chlorophylls, are potent photo-sensitizers. Plants usually accumulate these compounds bound to proteins together with protective compounds like carotenoids. Besides their protective role, carotenoids can play a structural role in these complexes. To analyze the ...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-006-0409-7
更新日期：2007-03-01 00:00:00
abstract：:On intact, 3-week-old plants of Phaseolus the larger bud in the axils of the primary leaves shows slow, continuous elongation growth. Release from correlative inhibition can be detected within 30 min following decapitation. When 0.1% indoleacetic acid in lanolin is applied to the decapitated stem stump, the lateral bu...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00383862
更新日期：1975-01-01 00:00:00
abstract：:Taking into account the literature on the so-called sieve-tube slime ("mictoplasm", "slime strands") and regarding its fine structure more in detail the term plasmatic filament ("Plasmafilament") is proposed and will be used in this paper to characterize the individual exceedingly fine subunit of the plasmatic network...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/BF00385169
更新日期：1967-03-01 00:00:00
abstract：:Fe uptake in dicotyledonous plants is mediated by a root plasma membrane-bound ferric reductase that reduces extracellular Fe(III)-chelates, releasing Fe(2+) ions, which are then absorbed via a metal ion transporter. We previously showed that Fe deficiency induces an increased capacity to absorb Fe and other micronutr...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-003-1156-7
更新日期：2004-03-01 00:00:00
abstract：:To study the interdependence of sucrose accumulation and its hydrolyzing enzyme, soluble acid invertase (AI; EC 3.2.1.26), in fructan-accumulating temperate grasses and cereals, experiments were performed in which sucrose synthesis was abolished in leaves of Lolium temulentum by four independent inhibitory factors, ea...
journal_title：Planta
pub_type： 杂志文章
doi：10.1007/s00425-004-1281-y
更新日期：2004-09-01 00:00:00
