The changes of antifungal susceptibilities caused by the phenotypic switching of Candida species in 229 patients with vulvovaginal candidiasis.

Abstract:

OBJECTIVE:This study was to investigate the changes of antifungal susceptibilities caused by the phenotypic switching in patients with vulvovaginal candidiasis (VVC). METHODS:229 women were enrolled in this study. The vaginal smears of these patients were collected and gram stained for fungal microscopic observation. The vaginal discharge of them in cotton swabs was cultured in sabouraud's agar with chloramphenicol medium. After fungal culture, fungal identification was analyzed using CHROM agar Candida chromogenic and identification medium. Then, the in vitro antifungal susceptibility testing was carried out using the standardized CLSI M27-A2 broth microdilution method. RESULTS:64.63% of Candia species in patients with VVC were Candida albicans and the remainders were non-albicans Candida species. The phenotypic switching was observed in 91.22% of C. albicans infection. In antifungal susceptibility testing, the susceptible rates of C. albicans to voriconazole, fluconazole and itraconazole were significantly higher than that of non-albicans Candida species (P = 0.00, 0.00, 0.00). No matters in patients infected with C. albicans or with non-albicans Candida species, the susceptible rate to fluconazole of the clinical isolates with phenotypic switching was significantly higher than that without phenotypic switching (P = 0.01, 0.01). CONCLUSIONS:In the study, C. albicans was the commonest pathogenic species in patients with VVC, in which the phenotypic switching was easy to occur. The susceptible rates of C. albicans to all antifungal drugs were higher than that of non-albicans Candida species. The susceptible rate to fluconazole was all influenced by the phenotypic switching in C. albicans and non-albicans Candida species.

journal_name

J Clin Lab Anal

authors

Tang Y,Yu F,Huang L,Hu Z

doi

10.1002/jcla.22644

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

e22644

issue

1

eissn

0887-8013

issn

1098-2825

journal_volume

33

pub_type

杂志文章
  • Role of TLR4 and miR-155 in peripheral blood mononuclear cell-mediated inflammatory reaction in coronary slow flow and coronary arteriosclerosis patients.

    abstract:BACKGROUND:To study the role of toll-like receptor 4 (TLR4) and MicroRNA-155 (miR-155) in the peripheral blood mononuclear cell (PBMC)-mediated inflammation in coronary slow flow (CSF) and coronary arteriosclerosis. METHODS:Patients were divided into acute coronary syndrome (ACS), stable angina pectoris (SAP), CSF, an...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22232

    authors: Huang J,Yang Q,He L,Huang J

    更新日期:2018-02-01 00:00:00

  • Platelet antibody analysis by three different tests.

    abstract:BACKGROUND:Platelet-reactive antibodies lead to thrombocytopenia and bleeding disorders, and diverse assays are used for their detection. In this retrospective analysis, the applicability of three different test systems was compared and antibody specificities were assessed. METHODS:Sera of 1,234 patients were tested w...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21750

    authors: Sareban N,Macher S,Drexler C,Posch U,Lanzer G,Schallmoser K

    更新日期:2015-05-01 00:00:00

  • Genetic analysis of chromosome 22q11.2 markers in congenital heart disease.

    abstract::Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.10062

    authors: Shi YR,Hsieh KS,Wu JY,Lee CC,Tsai CH,Yu MT,Chang JS,Tsai FJ

    更新日期:2003-01-01 00:00:00

  • Virulence factors impair epithelial junctions during bacterial infection.

    abstract::Epithelial cells are typically connected through different types of cell junctions that are localized from the apical membrane to the basal surface. In this way, epithelium cells form the first barrier against pathogenic microorganisms and prevent their entry into internal organs and the circulatory system. Recent stu...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章,评审

    doi:10.1002/jcla.23627

    authors: Zheng M,Sun S,Zhou J,Liu M

    更新日期:2020-10-17 00:00:00

  • Reliability of low-avidity IgG and of IgA in the diagnosis of primary infection by rubella virus with adaptation of a commercial test.

    abstract::The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modifi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:

    authors: Gutiérrez J,Rodríguez MJ,De Ory F,Piédrola G,Maroto MC

    更新日期:1999-01-01 00:00:00

  • Hormonal modulation of herpes simplex virus replication in a mouse neuroblastoma cell line.

    abstract::In this study, the effect of the synthetic glucocorticoid hormone dexamethasone (DXM) on HSV replication was studied in a DXM receptor-positive mouse neuroblastoma (NB) cell line. In cells treated with 10(-7) M DXM and then infected with HSV, there was a statistically significant 9-18-fold increase in the amount of vi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860080304

    authors: Sawiris GP,Sydiskis RJ,Bashirelahi N

    更新日期:1994-01-01 00:00:00

  • The impact of pre-analytical variations on biochemical analytes stability: A systematic review.

    abstract:OBJECTIVE:A common problem in clinical laboratories is maintaining the stability of analytes during pre-analytical processes. The aim of this study was to systematically summarize the results of a set of studies about the biochemical analytes stability. METHODS:A literature search was performed on the Advanced search ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章,评审

    doi:10.1002/jcla.23551

    authors: Hedayati M,Razavi SA,Boroomand S,Kheradmand Kia S

    更新日期:2020-09-01 00:00:00

  • Serotypes, antibiotic resistance, and virulence genes of Salmonella in children with diarrhea.

    abstract:BACKGROUND:Salmonella is an important foodborne pathogen that causes acute diarrhea in humans worldwide. This study analyzed the relationships of serotypes and antibiotic resistance with virulence genes of Salmonella isolated from children with salmonellosis. METHODS:Serological typing was performed using the slide-ag...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23525

    authors: Yue M,Li X,Liu D,Hu X

    更新日期:2020-08-14 00:00:00

  • Simultaneous measurement of neutrophil, lymphocyte, and monocyte glutathione by flow cytometry.

    abstract::A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominantly GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860040503

    authors: Scott RB,Collins JM,Matin S,White F,Swerdlow PS

    更新日期:1990-01-01 00:00:00

  • Performance characteristics of a transcription-mediated nucleic acid amplification assay for qualitative detection of hepatitis C virus RNA.

    abstract::The detection of hepatitis C virus (HCV) RNA by nucleic acid amplification techniques is the method of choice to differentiate between ongoing and past infection, and can be used to monitor the course of HCV infection. In this study, we evaluated the performance characteristics of a newly developed transcription-media...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1042

    authors: Ross RS,Viazov SO,Hoffmann S,Roggendorf M

    更新日期:2001-01-01 00:00:00

  • Predictive value of antinative DNA for systemic lupus erythematosus: comparison of radioimmunoassay and enzyme immunoassay.

    abstract::Positive predictive values for the diagnosis of systemic lupus erythematosus (SLE) were determined for two antinative DNA assays: a Farr-type radioimmunoassay (RIA) and a solid-phase enzyme immunoassay (EIA). The study population consisted of all patients whose EIA anti-DNA results were positive (greater than 250 IU) ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860030503

    authors: Clough JD,Chang RK

    更新日期:1989-01-01 00:00:00

  • Association between blood microbiome and type 2 diabetes mellitus: A nested case-control study.

    abstract:BACKGROUND:Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce. METHODS:Fifty incident T2DM cases and 100 matched non-T2DM controls were selected fr...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22842

    authors: Qiu J,Zhou H,Jing Y,Dong C

    更新日期:2019-05-01 00:00:00

  • Nucleic acid analysis by sandwich hybridization.

    abstract::One of the most significant achievements of the biochemist during the past two decades is the use to which immunologically based assays have been put in clinical diagnosis (Hood et al.: Immunology, 1984). The problem faced and surmounted by immunologists in effecting the transition from research tool to routine clinic...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章,评审

    doi:10.1002/jcla.1860030210

    authors: Nicholls PJ,Malcolm AD

    更新日期:1989-01-01 00:00:00

  • Useful information provided by graphic displays of automated cell counter in hematological malignancies.

    abstract:BACKGROUND:Automated cell counters have become more and more sophisticated with passing years. The numerical and graphic data both provide useful clues for suspecting a diagnosis especially when the workload is very high. AIM:We present our experience of useful information provided by graphic displays of an automated ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22392

    authors: Gupta M,Chauhan K,Singhvi T,Kumari M,Grover RK

    更新日期:2018-06-01 00:00:00

  • Analysis of serum gastrin-17 and Helicobacter pylori antibody in healthy Chinese population.

    abstract:BACKGROUND:Gastrin-17 (G-17) and Helicobacter pylori (H pylori) antibody are widely used in the screening of gastric diseases, especially in gastric cancer. In this study, we aimed to evaluate the value of G-17 and H pylori antibody in gastric disease screening. METHODS:Healthy males and females (1368 and 1212, respec...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23518

    authors: Liu W,Sun Y,Yuan Y

    更新日期:2020-08-30 00:00:00

  • Ischemia-modified albumin in acute aortic dissection.

    abstract:BACKGROUND:Acute aortic dissection (AOD) is associated with high mortality and early diagnosis and treatment are essential. Ischemia-modified albumin (IMA) is a marker of myocardial ischemia whereas cardiac enzymes are released when myocardial necrosis occurs. We investigated, for the first time, whether IMA increases ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20418

    authors: Sbarouni E,Georgiadou P,Marathias A,Panagiotakos D,Geroulanos S,Voudris V

    更新日期:2010-01-01 00:00:00

  • Increased serum adiponectin predicts improved coronary flow and clinical outcomes in patients with ST-segment elevation myocardial infarction treated by primary percutaneous coronary intervention.

    abstract:BACKGROUND:Previous studies suggested that adiponectin (APN) could ameliorate ischemia/reperfusion injury and endothelial dysfunction in patients with acute myocardial infarction. However, the relationship between serum APN level and coronary flow after primary percutaneous coronary intervention (PPCI) in patients with...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 临床试验,杂志文章

    doi:10.1002/jcla.22864

    authors: Liang S,Li H,Shen X,Liu R

    更新日期:2019-06-01 00:00:00

  • Twelve years of the Brazilian External Quality Assessment Program in Immunohematology: benefits of the program.

    abstract::The Brazilian External Quality Assessment Program in Immunohematology (BEQAPI) was introduced with the objective of evaluating the quality of diagnosis in immunohematology. From 1992 to 2003, proficiency tests for ABO grouping, Rh (D, C, c, E, e), K phenotyping, direct antiglobulin testing (DAT), antibody screening (A...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20080

    authors: Melo L,Pellegrino J Jr,Bianco C,Castilho L

    更新日期:2005-01-01 00:00:00

  • Detection of submicroscopic chromosomal aberrations by chromosomal microarray analysis for the prenatal diagnosis of central nervous system abnormalities.

    abstract:BACKGROUND:Central nervous system (CNS) abnormalities are a group of serious birth defects associated with high rates of stillbirths, infant death, or abnormal development, and various disease-causing copy number variations play a much more important role in the etiology of CNS abnormalities. This study intends to pres...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23434

    authors: Song T,Xu Y,Li Y,Jia L,Zheng J,Dang Y,Wan S,Zheng Y,Zhang J,Yang H

    更新日期:2020-10-01 00:00:00

  • Performance Evaluation of a Salivary Amylase Biosensor for Stress Assessment in Military Field Research.

    abstract:BACKGROUND:A convenient biosensor for real-time measurement of biomarkers for in-field psychophysiological stress research and military operations is desirable. We evaluated a hand-held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two dif...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21840

    authors: Peng HT,Savage E,Vartanian O,Smith S,Rhind SG,Tenn C,Bjamason S

    更新日期:2016-05-01 00:00:00

  • Preliminary study of the relationship between promoter methylation of the ANGPTL2 gene and coronary heart disease.

    abstract:BACKGROUND:Coronary heart disease (CHD) is primarily caused by atherosclerosis of coronary arteries. It is largely an inflammatory disease of the vascular wall. The inflammation is related to DNA methylation. Angiopoietin-like protein 2 (ANGPTL2) has various functions in several chronic inflammatory diseases. Macrophag...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22702

    authors: Zhou J,Chen L,Yang X,Huang X,Wang Z,Peng P,Lian J

    更新日期:2019-03-01 00:00:00

  • Transferrin D protein variants in the diagnosis of congenital disorders of glycosylation (CDG).

    abstract::Congenital disorders of glycosylation are a rapidly growing group of inherited (neuro)metabolic disorders characterized by defects in glycosylation of proteins and lipids. This study discusses an analytical problem in the differentiation between hypoglycosylation and transferrin (Tf) protein variants. Analysis of seru...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20293

    authors: Marklová E,Albahri Z

    更新日期:2009-01-01 00:00:00

  • Rapid and sensitive headspace gas chromatography-mass spectrometry method for the analysis of ethanol in the whole blood.

    abstract:BACKGROUND:For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min. ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21698

    authors: Xiao HT,He L,Tong RS,Yu JY,Chen L,Zou J,Li JQ,Bian Y,Zhang Y

    更新日期:2014-09-01 00:00:00

  • New proposed guidelines for early identification of successful myeloid and erythroid engraftment in hematopoietic stem cell transplantation.

    abstract:BACKGROUND:After hematopoietic stem cell transplantation (HSCT), early detection of engraftment would be of critical value for clinicians. The aim of this study was to identify faster parameters for engraftment. METHODS:We evaluated blood cell parameters including complete blood count (CBC), differential counts, and v...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21712

    authors: Yahng SA,Lee JW,Kim Y,Kim M,Oh EJ,Park YJ,Lee JW,Cho B,Han K

    更新日期:2014-11-01 00:00:00

  • The microarray identification circular RNA hsa_circ_0105015 up-regulated involving inflammation pathway in essential hypertension.

    abstract:BACKGROUND:Essential hypertension (EH) is an inflammatory disease, and endothelial dysfunction induced by chronic inflammation is one of the pathogeneses of EH. The expression of some inflammatory mediators may be regulated by the interaction of circular RNAs (circRNAs) and microRNAs (miRNAs). METHODS:An Agilent human...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23603

    authors: He X,Bao X,Tao Z,Sun J,Zheng S,Zhong F,Zhang L

    更新日期:2020-11-24 00:00:00

  • The importance of serum biglycan levels as a fibrosis marker in patients with chronic hepatitis B.

    abstract:BACKGROUND:Liver biopsy is recommended in the majority of patients with chronic viral hepatitis for fibrosis evaluation. Because of the potential risks of liver biopsy, many studies related to non-invasive biomarkers of hepatic fibrosis have been performed. We aimed to assess the diagnostic value of serum biglycan as a...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22109

    authors: Ciftciler R,Ozenirler S,Yucel AA,Cengiz M,Erkan G,Buyukdemirci E,Sönmez C,Esendaglı GY

    更新日期:2017-09-01 00:00:00

  • Multivariable index for assessing the activity and predicting all-cause mortality in antineutrophil cytoplasmic antibody-associated vasculitis.

    abstract:BACKGROUND:So far, there has been no tool to estimate activity at diagnosis and predict all-cause mortality in patients with ANCA-associated vasculitis (AAV). Hence, we determined the initial predictors of them in patients with AAV. METHODS:We retrospectively reviewed the medical records of 182 patients with AAV. Seve...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23022

    authors: Kim MK,Choi H,Kim JY,Song JJ,Park YB,Lee SW

    更新日期:2020-01-01 00:00:00

  • Broad-spectrum next-generation sequencing-based diagnosis of a case of Nager syndrome.

    abstract:BACKGROUND:Nager syndrome is a rare genetic syndrome characterized by craniofacial and preaxial limb anomalies. Haploinsufficiency of the SF3B4 gene has been identified as a significant reason for Nager syndrome. Treacher Collins syndrome (TCS) has similar facial features; however, the TCOF1, POLR1D, and POLR1C genes h...

    journal_title:Journal of clinical laboratory analysis

    pub_type:

    doi:10.1002/jcla.23426

    authors: Zhao J,Yang L

    更新日期:2020-09-01 00:00:00

  • Identification of potential metabolite markers for colon cancer and rectal cancer using serum metabolomics.

    abstract:BACKGROUND:To determine the metabolic characteristics of patients with colon cancer (CC) and rectal cancer (RC) using gas chromatography-mass spectrometry (GC-MS)-based metabolomics. METHODS:In this study, serum samples were collected from 22 CC patients and 23 RC patients preoperatively and postoperatively and 45 hea...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23333

    authors: Wu J,Wu M,Wu Q

    更新日期:2020-08-01 00:00:00

  • Co-sensitization and cross-reactivity of Blomia tropicalis with two Dermatophagoides species in Guangzhou, China.

    abstract:BACKGROUND:Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22981

    authors: Liu X,Zheng P,Zheng SG,Zhai Y,Zhao X,Chen Y,Cai C,Wu Z,Huang Z,Zou X,Liao C,Sun B

    更新日期:2019-11-01 00:00:00