Abstract:
:We have characterized opioid binding sites in the Sertoli cells of adult and 18-day-old rat testes. Maximal specific etorphine binding was attained after 30 min at 4 C. The binding was reversible, with association and dissociation rate constants of 0.98 X 10(5) M-1 min-1 and 0.33 min-1, respectively. Scatchard analyses and saturation curves revealed a single class of high-affinity, low-capacity binding sites. No opioid binding was observed in Leydig cell cultures. Exposure to opioids for 3 days caused a significant increase in [3H]etorphine specifically bound to the Sertoli cells that was completely prevented by naloxone, demonstrating opioid up-regulation of its own receptor. Chronic opioid treatment of the cultures significantly inhibited androgen-binding protein production, and this effect was prevented by naloxone. Since the circulating concentrations of endorphins (10(-12) M) are lower than the Kd of testis opiate receptors, it is conceivable that opioids of Leydig cell origin act on the specific high-affinity receptors of the Sertoli cells, and may play a role in modulating their function.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Fabbri A,Tsai-Morris CH,Luna S,Fraioli F,Dufau MLdoi
10.1210/endo-117-6-2544subject
Has Abstractpub_date
1985-12-01 00:00:00pages
2544-6issue
6eissn
0013-7227issn
1945-7170journal_volume
117pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Exposure to testosterone (T) during d 30-90 of fetal life results in low-birth-weight offspring, hypergonadotropism, multifollicular ovaries, and early cessation of cyclicity. The multifollicular phenotype may result from failure of follicles to regress and consequent follicular persistence or, alternatively, increase...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1444
更新日期:2005-07-01 00:00:00
abstract::The establishment of dominant ovarian follicles that are capable of ovulating fertilizable oocytes is a fundamental determinant of female fertility. This process is governed by pituitary gonadotropins as well as local ovarian factors. Within the follicle, estrogen acts in an autocrine/paracrine manner to enhance FSH a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0349
更新日期:2005-08-01 00:00:00
abstract::The ileal proglucagon-derived peptides (PGDPs) play important roles in the regulation of gastric function and insulin secretion. We have previously demonstrated the existence of a proximal-distal enteroendocrine loop mediated by glucose-dependent insulinotropic peptide (GIP), through which intestinal PGDP secretion is...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.6.8641190
更新日期:1996-06-01 00:00:00
abstract::In the ovarian follicle, oocyte-secreted factors induce cumulus-specific genes and repress mural granulosa cell specific genes to establish these functionally distinct cell lineages. The mechanism establishing this precise morphogenic pattern of oocyte signaling within the follicle is unknown. The present study invest...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1181
更新日期:2012-09-01 00:00:00
abstract::RNA blot hybridization analysis with cloned rat CRF precursor (prepro-CRF) cDNA as a probe showed that prepro-CRF mRNA existed in rat hypothalamic and extrahypothalamic brain tissue, whereas it was undetectable in the pituitary and adrenal. To study the effect of glucocorticoid on the level of prepro-CRF mRNA in the h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1314
更新日期:1985-10-01 00:00:00
abstract::ACTH and cAMP stimulate steroidogenesis and the mitochondrial electron transport system for steroid hydroxylation in cultured mouse adrenal cortex tumor cells. During this stimulation, the biosynthesis of adrenodoxin, a non-heme iron protein which is one of the electron transport enzymes, was examined. 14C-labeled adr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-99-4-977
更新日期:1976-10-01 00:00:00
abstract::The anterior pituitary (AP) is made of five different cell types. The relative abundance and phenotype of AP cells may change in different physiological situations as an expression of pituitary plasticity. Here, we analyze in detail the phenotype of mouse corticotropes and the effects of acute cold stress on AP cell p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1030
更新日期:2008-05-01 00:00:00
abstract::The existence of renin in a number of extrarenal tissues has been well documented, but the physiological role of extrarenal renin remains unknown. To study the physiological role of adrenal renin, we developed a serum-free culture system for adrenal capsular/zona glomrulosa explants. Explants showed good viability in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-1-486
更新日期:1989-07-01 00:00:00
abstract::The antral hormone gastrin and its intestinal relative, cholecystokinin (CCK), are pivotal in the regulation of gastric functions. Other gastric hormones like ghrelin, peptide YY (PYY), and islet amyloid polypeptide (IAPP), however, also contribute to the regulation of acid secretion, motility, and feeding. Because ga...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0938
更新日期:2005-10-01 00:00:00
abstract::Hypothalamic inflammation and gliosis are proposed to participate in the pathogenesis of high-fat diet-induced obesity. Because other factors and nutrients also induce weight gain and adiposity, we analyzed the inflammatory and glial responses to a sucrose (S)-enriched diet. Neonatal overnutrition (NON) exacerbates we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-2196
更新日期:2013-07-01 00:00:00
abstract::Although the extent of proptosis in exophthalmic Graves' disease has been measured directly and shown to correlate with serum content of a bioassayable exophthalmus-producing factor (EPS;1), a comparable relationship in an experimental model has not been reported. Progressive exophthalmos, measured from photographs an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-1-151
更新日期:1975-01-01 00:00:00
abstract::Estradiol-17beta (E2) is suspected to exert a role in the regulation of testicular activity. Using a nonmammalian vertebrate model (the frog, Rana esculenta), we have investigated whether c-fos activity is detectable in the testis during the annual sexual cycle and whether E2 exerts a regulatory role on spermatogenesi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.7.6790
更新日期:1999-07-01 00:00:00
abstract::The expression of cholesterol side-chain cleavage cytochrome P450 (P450scc), 17 alpha-hydroxylase cytochrome P450 (P45017 alpha), and 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) was studied in bovine placenta and fetal adrenal glands throughout gestation. The levels of expression of these enzymes were much lower ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.5.1374010
更新日期:1992-05-01 00:00:00
abstract::Dehydroepiandrosterone (DHEA) fed at 0.4% in the diet is known to exert strong antihyperglycemic effects in C57BL/KsJ genetically diabetic (db/db) mice. Three of the major metabolic products of DHEA; DHEA sulfate, alpha-hydroxyetiocholanolone (alpha-ET), and beta-hydroxyetiocholanolone (beta-ET) when fed at 0.1% in th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-239
更新日期:1984-07-01 00:00:00
abstract::In humans, there is a significant decrease in serum T(3) and increase in rT(3) at different time points after myocardial infarction, whereas serum TSH and T(4) remain unaltered. We report here a time course study of pituitary-thyroid function and thyroid hormone metabolism in rats subjected to myocardial infarction by...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0043
更新日期:2007-10-01 00:00:00
abstract::Calcitonin (CT) and related peptides, such as CT gene-related peptide and salmon CT (sCT)-like peptide, are present in the rat nervous system and the pituitary gland, and sCT markedly inhibits basal and TRH-stimulated PRL release from anterior pituitary (AP) cells. Because TRH-induced PRL release is known to involve i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-127-2-613
更新日期:1990-08-01 00:00:00
abstract::Acromegaly is associated with an increased incidence of cardiovascular disease. Transgenic mice expressing bovine GH (bGH) gene have previously been used to examine the effects of chronic GH stimulation on cardiovascular function. Results concerning systolic blood pressure (SBP) in bGH mice are conflicting. We hypothe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1899
更新日期:2014-03-01 00:00:00
abstract::The prevailing view of sexual differentiation of mammalian brain is that androgen synthesized in the fetal and neonatal testis and aromatized centrally during a perinatal sensitive period is the sole source of brain estradiol and the primary determinant of sex differences. Subregions of the diencephalon are among the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0607
更新日期:2011-01-01 00:00:00
abstract::LH supplementation in assisted reproductive technology cycles improves the ongoing pregnancy rate in women with poor ovarian response (POR). However, our knowledge of the precise role of LH during the follicular phase of the menstrual cycle is incomplete. To explore the role of LH in the maturation of small antral fol...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00123
更新日期:2018-06-01 00:00:00
abstract::It is well established that LH has an obligatory role in the acute production of progesterone by the primate corpus luteum in vivo because interruption of LH support to the corpus luteum at any time during the luteal phase is accompanied by an immediate and sustained fall in serum progesterone concentrations. However,...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.5.1425410
更新日期:1992-11-01 00:00:00
abstract::Fibroblast growth factor (FGF) 21, a unique, largely liver-derived endocrine member of the FGF superfamily, is often thought of as a fasting factor owing to its induction in rodents during starvation. However, FGF21 is not increased by fasting for periods of <7 days in humans; instead, it rises sharply after acute alc...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2018-00208
更新日期:2018-05-01 00:00:00
abstract::To examine the mechanism of estrogen's direct stimulation of steroidogenesis in the rabbit corpus luteum, we tested the hypothesis that the effect of estrogen on progestin production occurs at the site of processing of the precursor for pregnenolone (i.e. cholesterol) in the mitochondrion. For this purpose, we manipul...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-1948
更新日期:1988-05-01 00:00:00
abstract::PTH stimulates synthesis and secretion of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in renal proximal tubule cells through activation of the protein kinase-A (PKA) or the protein kinase-C (PKC) signaling pathway. The relative contribution of the two transducing systems was explored using PTH fragments with selective act...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.2.8344210
更新日期:1993-08-01 00:00:00
abstract::Studies were undertaken using the opiate receptor antagonist naloxone (NAL) to evaluate the relative influence of endogenous opioid peptides (EOP) on LH secretion in cycling and ovariectomized, steroid-treated adult rats. Intact animals received NAL (2 mg/kg, sc) or saline (control vehicle) at 0800 and 1400 h on estru...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-5-1806
更新日期:1983-11-01 00:00:00
abstract::In the present study, steroidogenesis in two different Leydig tumor cell lines was compared. One, the MA-10 mouse tumor cell line, produces steroids and the steroidogenic acute regulatory (StAR) protein only when stimulated by trophic hormones and cAMP analogs. The other, the R2C rat tumor cell line, produces steroids...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0941
更新日期:2004-12-01 00:00:00
abstract::31P nuclear magnetic resonance (NMR) was used to study the effects of 17 beta-estradiol on the content of phosphates and on the flux catalyzed by creatine kinase in immature rat uteri. Perifusion with oxygenated medium at 36 C maintained the uteri in a viable state for at least 10 h in vitro during 31P NMR measurement...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-4-1631
更新日期:1988-04-01 00:00:00
abstract::Neuropeptide Y (NPY) is a powerful orexigenic factor, and alphaMSH is a melanocortin (MC) peptide that induces satiety by activating the MC4 receptor subtype. Genetic models with disruption of MC4 receptor signaling are associated with obesity. In the present study, a 7-day intracerebroventricular infusion to male rat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.12.7842
更新日期:2000-12-01 00:00:00
abstract::Human livers, obtained from donors at the time of transplant, were homogenized in 0.25 M sucrose and fractionated by differential centrifugation. The specific binding of [125I] human (h) GH to total particulate fractions from 18 livers varied from 0.4-5.1% of the total radioactivity/100 micrograms protein. Binding aff...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-4-2167
更新日期:1989-10-01 00:00:00
abstract::Regulation of galanin gene expression in the anterior pituitary (AP) is positively influenced by estrogen in rodents and undetermined in humans. The objective of this study was to investigate the mechanism behind estrogen induction of galanin by identifying any putative estrogen receptor (ER) binding sequences within ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.11.5507
更新日期:1997-11-01 00:00:00
abstract::In previous work we showed that the chondrodysplastic phenotype of mice homozygous for a null mutation of the PTH-related peptide (PTHrP) gene was due in part to reduced proliferation and aberrant differentiation of growth plate chondrocytes. In the present study we have extended those observations by examining chondr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.11.8895380
更新日期:1996-11-01 00:00:00