A ten-year search for synchronous cells: obstacles, solutions, and practical applications.

Abstract:

:My effort to use synchronously dividing cultures to examine the Escherichia coli cell cycle involved a 10-year struggle with failure after failure punctuated by a few gratifying successes, especially at the end. In this essay, I recount my personal journey in this obsessive experimental pursuit. That narrative is followed by a description of a simplified version of the "baby machine," a technique that was developed to obtain minimally disturbed, synchronously growing E. coli cells. Subsequent studies with this methodology led to an understanding of the basic properties of the relationship between chromosome replication and cell division. Accordingly, I end this reminiscence with a simple, fool-proof graphical strategy for deducing the pattern of chromosome replication during the division cycle of cells growing at any rate.

journal_name

Front Microbiol

authors

Helmstetter CE

doi

10.3389/fmicb.2015.00238

subject

Has Abstract

pub_date

2015-03-25 00:00:00

pages

238

issn

1664-302X

journal_volume

6

pub_type

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