Characterization and Testing the Efficiency of Acinetobacter baumannii Phage vB-GEC_Ab-M-G7 as an Antibacterial Agent.

Abstract:

:Acinetobacter baumannii is a gram-negative, non-motile bacterium that, due to its multidrug resistance, has become a major nosocomial pathogen. The increasing number of multidrug resistant (MDR) strains has renewed interest in phage therapy. The aim of our study was to assess the effectiveness of phage administration in Acinetobacter baumannii wound infections in an animal model to demonstrate phage therapy as non-toxic, safe and alternative antibacterial remedy. Using classical methods for the study of bacteriophage properties, we characterized phage vB-GEC_Ab-M-G7 as a dsDNA myovirus with a 90 kb genome size. Important characteristics of vB-GEC_Ab-M-G7include a short latent period and large burst size, wide host range, resistance to chloroform and thermal and pH stability. In a rat wound model, phage application effectively decreased the number of bacteria isolated from the wounds of successfully treated animals. This study highlights the effectiveness of the phage therapy and provides further insight into treating infections caused by MDR strains using phage administration.

journal_name

Front Microbiol

authors

Kusradze I,Karumidze N,Rigvava S,Dvalidze T,Katsitadze M,Amiranashvili I,Goderdzishvili M

doi

10.3389/fmicb.2016.01590

subject

Has Abstract

pub_date

2016-10-04 00:00:00

pages

1590

issn

1664-302X

journal_volume

7

pub_type

杂志文章
  • Transmissible ST3-IncHI2 Plasmids Are Predominant Carriers of Diverse Complex IS26-Class 1 Integron Arrangements in Multidrug-Resistant Salmonella.

    abstract::Diverse mobile genetic elements (MGEs) including plasmids, insertion sequences, and integrons play an important role in the occurrence and spread of multidrug resistance (MDR) in bacteria. It was found in previous studies that IS26 and class 1 integrons integrated on plasmids to speed the dissemination of antibiotic-r...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02492

    authors: Zhao H,Chen W,Xu X,Zhou X,Shi C

    更新日期:2018-10-23 00:00:00

  • The Diverse Functional Roles of Elongation Factor Tu (EF-Tu) in Microbial Pathogenesis.

    abstract::Elongation factor thermal unstable Tu (EF-Tu) is a G protein that catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome inside living cells. Structural and biochemical studies have described the complex interactions needed to effect canonical function. However, EF-Tu has evolved the capacity to execute...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.02351

    authors: Harvey KL,Jarocki VM,Charles IG,Djordjevic SP

    更新日期:2019-10-24 00:00:00

  • Endophytic Bacterial Community Structure and Function of Herbaceous Plants From Petroleum Hydrocarbon Contaminated and Non-contaminated Sites.

    abstract::Bacterial endophytes (BEs) are non-pathogenic residents of healthy plant tissues that can confer benefits to plants. Many Bacterial endophytes have been shown to contribute to plant growth and health, alleviation of plant stress and to in-planta contaminant-degradation. This study examined the endophytic bacterial com...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01926

    authors: Lumactud R,Fulthorpe RR

    更新日期:2018-08-23 00:00:00

  • Streptococcus suis 2 Transcriptional Regulator TstS Stimulates Cytokine Production and Bacteremia to Promote Streptococcal Toxic Shock-Like Syndrome.

    abstract::Two large-scale outbreaks of streptococcal toxic shock-like syndrome (STSLS) have revealed Streptococcus suis 2 to be a severe and evolving human pathogen. We investigated the mechanism by which S. suis 2 causes STSLS. The transcript abundance of the transcriptional regulator gene tstS was found to be upregulated duri...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01309

    authors: Xu Z,Chen B,Zhang Q,Liu L,Zhang A,Yang Y,Huang K,Yan S,Yu J,Sun X,Jin M

    更新日期:2018-06-19 00:00:00

  • Connecting lignin-degradation pathway with pre-treatment inhibitor sensitivity of Cupriavidus necator.

    abstract::To produce lignocellulosic biofuels economically, the complete release of monomers from the plant cell wall components, cellulose, hemicellulose, and lignin, through pre-treatment and hydrolysis (both enzymatic and chemical), and the efficient utilization of these monomers as carbon sources, is crucial. In addition, t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00247

    authors: Wang W,Yang S,Hunsinger GB,Pienkos PT,Johnson DK

    更新日期:2014-05-27 00:00:00

  • Early Ongoing Speciation of Ogataea uvarum Sp. Nov. Within the Grape Ecosystem Revealed by the Internal Variability Among the rDNA Operon Repeats.

    abstract::A yeast strain was isolated during a study on vineyard-associated yeast strains from Apulia in Southern Italy. ITS and LSU D1/D2 rDNA sequences showed this strain not to belong to any known species and was described as the type strain of Ogataea uvarum sp. nov., a close relative of O. philodendri. Several secondary pe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01687

    authors: Roscini L,Tristezza M,Corte L,Colabella C,Perrotta C,Rampino P,Robert V,Vu D,Cardinali G,Grieco F

    更新日期:2018-08-03 00:00:00

  • xCT, not just an amino-acid transporter: a multi-functional regulator of microbial infection and associated diseases.

    abstract::Expression of xCT, a component of the xc (-) amino-acid transporter, is essential for the uptake of cystine required for intracellular glutathione (GSH) synthesis and maintenance of the intracellular redox balance. Therefore, xCT plays an important role not only in the survival of somatic and immune cells, but also in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00120

    authors: Dai L,Noverr MC,Parsons C,Kaleeba JA,Qin Z

    更新日期:2015-02-19 00:00:00

  • The RstAB System Impacts Virulence, Motility, Cell Morphology, Penicillin Tolerance and Production of Type II Secretion System-Dependent Factors in the Fish and Human Pathogen Photobacterium damselae subsp. damselae.

    abstract::The RstB histidine kinase of the two component system RstAB positively regulates the expression of damselysin (Dly), phobalysin P (PhlyP) and phobalysin C (PhlyC) cytotoxins in the fish and human pathogen Photobacterium damselae subsp. damselae, a marine bacterium of the family Vibrionaceae. However, the function of t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00897

    authors: Terceti MS,Vences A,Matanza XM,Barca AV,Noia M,Lisboa J,Dos Santos NMS,do Vale A,Osorio CR

    更新日期:2019-04-24 00:00:00

  • Inhibition of Rumen Methanogens by a Novel Archaeal Lytic Enzyme Displayed on Tailored Bionanoparticles.

    abstract::Methane is a potent greenhouse gas, 25 times more efficient at trapping heat than carbon dioxide. Ruminant methane emissions contribute almost 30% to anthropogenic sources of global atmospheric methane levels and a reduction in methane emissions would significantly contribute to slowing global temperature rises. Here ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02378

    authors: Altermann E,Schofield LR,Ronimus RS,Beatty AK,Reilly K

    更新日期:2018-10-09 00:00:00

  • Microbial Community Structure and the Persistence of Cyanobacterial Populations in Salt Crusts of the Hyperarid Atacama Desert from Genome-Resolved Metagenomics.

    abstract::Although once thought to be devoid of biology, recent studies have identified salt deposits as oases for life in the hyperarid Atacama Desert. To examine spatial patterns of microbial species and key nutrient sources, we genomically characterized 26 salt crusts from three sites along a fog gradient. The communities ar...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01435

    authors: Finstad KM,Probst AJ,Thomas BC,Andersen GL,Demergasso C,Echeverría A,Amundson RG,Banfield JF

    更新日期:2017-07-28 00:00:00

  • Long-term impact of farm management and crops on soil microorganisms assessed by combined DGGE and PLFA analyses.

    abstract::In the present study, long-term organic and conventional managements were compared at the experimental field of Monsampolo del Tronto (Marche region, Italy) with the aim of investigating soil chemical fertility and microbial community structure. A polyphasic approach, combining soil fertility indicators with microbiol...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00644

    authors: Stagnari F,Perpetuini G,Tofalo R,Campanelli G,Leteo F,Della Vella U,Schirone M,Suzzi G,Pisante M

    更新日期:2014-12-10 00:00:00

  • Fungal ITS1 Deep-Sequencing Strategies to Reconstruct the Composition of a 26-Species Community and Evaluation of the Gut Mycobiota of Healthy Japanese Individuals.

    abstract::The study of mycobiota remains relatively unexplored due to the lack of sufficient available reference strains and databases compared to those of bacterial microbiome studies. Deep sequencing of Internal Transcribed Spacer (ITS) regions is the de facto standard for fungal diversity analysis. However, results are often...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00238

    authors: Motooka D,Fujimoto K,Tanaka R,Yaguchi T,Gotoh K,Maeda Y,Furuta Y,Kurakawa T,Goto N,Yasunaga T,Narazaki M,Kumanogoh A,Horii T,Iida T,Takeda K,Nakamura S

    更新日期:2017-02-15 00:00:00

  • Beyond phage display: non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic, and bioconjugation scaffold.

    abstract::For the past 25 years, phage display technology has been an invaluable tool for studies of protein-protein interactions. However, the inherent biological, biochemical, and biophysical properties of filamentous bacteriophage, as well as the ease of its genetic manipulation, also make it an attractive platform outside t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00755

    authors: Henry KA,Arbabi-Ghahroudi M,Scott JK

    更新日期:2015-08-04 00:00:00

  • Coral-Associated Bacterial Diversity Is Conserved across Two Deep-Sea Anthothela Species.

    abstract::Cold-water corals, similar to tropical corals, contain diverse and complex microbial assemblages. These bacteria provide essential biological functions within coral holobionts, facilitating increased nutrient utilization and production of antimicrobial compounds. To date, few cold-water octocoral species have been ana...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00458

    authors: Lawler SN,Kellogg CA,France SC,Clostio RW,Brooke SD,Ross SW

    更新日期:2016-04-05 00:00:00

  • Insights in the Global Genetics and Gut Microbiome of Black Soldier Fly, Hermetia illucens: Implications for Animal Feed Safety Control.

    abstract::The utilization of the black soldier fly (BSF) Hermetia illucens L. for recycling organic waste into high-quality protein and fat biomass for animal feeds has gained momentum worldwide. However, information on the genetic diversity and environmental implications on safety of the larvae is limited. This study delineate...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01538

    authors: Khamis FM,Ombura FLO,Akutse KS,Subramanian S,Mohamed SA,Fiaboe KKM,Saijuntha W,Van Loon JJA,Dicke M,Dubois T,Ekesi S,Tanga CM

    更新日期:2020-07-07 00:00:00

  • Early Trypanosoma cruzi Infection Triggers mTORC1-Mediated Respiration Increase and Mitochondrial Biogenesis in Human Primary Cardiomyocytes.

    abstract::Chagasic chronic cardiomyopathy is one of the most frequent and severe manifestations of Chagas disease, caused by the parasite Trypanosoma cruzi. The pathogenic and biochemical mechanisms responsible for cardiac lesions remain not completely understood, although it is clear that hypertrophy and subsequent heart dilat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01889

    authors: Libisch MG,Faral-Tello P,Garg NJ,Radi R,Piacenza L,Robello C

    更新日期:2018-08-16 00:00:00

  • Insight Into Function and Subcellular Localization of Plasmopara viticola Putative RxLR Effectors.

    abstract::Grapevine downy mildew, caused by oomycete fungus Plasmopara viticola, is one of the most devastating diseases of grapes across the major production regions of the world. Although many putative effector molecules have been identified from this pathogen, the functions of the majority of these are still unknown. In this...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00692

    authors: Chen T,Liu R,Dou M,Li M,Li M,Yin X,Liu GT,Wang Y,Xu Y

    更新日期:2020-04-21 00:00:00

  • Nutrient Dependent Cross-Kingdom Interactions: Fungi and Bacteria From an Oligotrophic Desert Oasis.

    abstract::Microbial interactions play a key role in ecosystem functioning, with nutrient availability as an important determinant. Although phylogenetically distant bacteria and fungi commonly co-occur in nature, information on their cross-kingdom interactions under unstable, extreme environments remains poor. Hence, the aims o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01755

    authors: Velez P,Espinosa-Asuar L,Figueroa M,Gasca-Pineda J,Aguirre-von-Wobeser E,Eguiarte LE,Hernandez-Monroy A,Souza V

    更新日期:2018-08-06 00:00:00

  • Immune Protection of Rhoptry Protein 21 (ROP21) of Toxoplasma gondii as a DNA Vaccine Against Toxoplasmosis.

    abstract::Toxoplasma gondii rhoptry proteins (TgROPs) are the major targets as key molecules for immunodiagnosis as well as immunoprophylaxis because of their initial presentation to the host immune system. In this work, it was aimed at evaluating the protection effect of TgROP21 DNA vaccine on experimental mice subjected to T....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00909

    authors: Zhang Z,Li Y,Wang M,Xie Q,Li P,Zuo S,Kong L,Wang C,Wang S

    更新日期:2018-05-08 00:00:00

  • Potential Biological and Climatic Factors That Influence the Incidence and Persistence of Highly Pathogenic H5N1 Avian Influenza Virus in Egypt.

    abstract::Highly pathogenic H5N1 avian influenza virus (A/H5N1) of clade 2.2.1 is endemic in poultry in Egypt where the highest number of human infections worldwide was reported. During the last 12 years the Egyptian A/H5N1 evolved into several genotypes. In 2007-2014 vaccinated poultry suffered from antigenic drift variants of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00528

    authors: Salaheldin AH,Kasbohm E,El-Naggar H,Ulrich R,Scheibner D,Gischke M,Hassan MK,Arafa AA,Hassan WM,Abd El-Hamid HS,Hafez HM,Veits J,Mettenleiter TC,Abdelwhab EM

    更新日期:2018-03-27 00:00:00

  • High-Throughput Proteomics Identifies Proteins With Importance to Postantibiotic Recovery in Depolarized Persister Cells.

    abstract::Bacterial populations produce phenotypic variants called persisters to survive harmful conditions. Persisters are highly tolerant to antibiotics and repopulate environments after the stress has vanished. In order to resume growth, persisters have to recover from the persistent state, but the processes behind recovery ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00378

    authors: Spanka DT,Konzer A,Edelmann D,Berghoff BA

    更新日期:2019-03-06 00:00:00

  • Activity, Abundance, and Localization of Quorum Sensing Receptors in Vibrio harveyi.

    abstract::Quorum sensing (QS) is a process enabling a bacterial population to communicate via small molecules called autoinducers (AIs). This intercellular communication process allows single cells to synchronize their behavior within a population. The marine bacterium Vibrio harveyi ATCC BAA-1116 channels the information of th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00634

    authors: Lorenz N,Shin JY,Jung K

    更新日期:2017-04-18 00:00:00

  • The RpoS Gatekeeper in Borrelia burgdorferi: An Invariant Regulatory Scheme That Promotes Spirochete Persistence in Reservoir Hosts and Niche Diversity.

    abstract::Maintenance of Borrelia burgdorferi within its enzootic cycle requires a complex regulatory pathway involving the alternative σ factors RpoN and RpoS and two ancillary trans-acting factors, BosR and Rrp2. Activation of this pathway occurs within ticks during the nymphal blood meal when RpoS, the effector σ factor, tra...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01923

    authors: Caimano MJ,Groshong AM,Belperron A,Mao J,Hawley KL,Luthra A,Graham DE,Earnhart CG,Marconi RT,Bockenstedt LK,Blevins JS,Radolf JD

    更新日期:2019-08-21 00:00:00

  • Emerging Trends in Biological Treatment of Wastewater From Unconventional Oil and Gas Extraction.

    abstract::Unconventional oil and gas exploration generates an enormous quantity of wastewater, commonly referred to as flowback and produced water (FPW). Limited freshwater resources and stringent disposal regulations have provided impetus for FPW reuse. Organic and inorganic compounds released from the shale/brine formation, m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.569019

    authors: Acharya SM,Chakraborty R,Tringe SG

    更新日期:2020-09-09 00:00:00

  • Identification of Genetic Variants via Bacterial Respiration Gas Analysis.

    abstract::Indole is a signal molecule derived from the conversion of tryptophan, and it is present in bacterial respiratory gas. Besides influencing bacterial growth, indole exhibits effects on human health, including a positive effect on inflammation and protection against pathogens. However, a high fecal indole concentration ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.581571

    authors: Koga N,Hosomi T,Zwama M,Jirayupat C,Yanagida T,Nishino K,Yamasaki S

    更新日期:2020-11-16 00:00:00

  • Growth and membrane fluidity of food-borne pathogen Listeria monocytogenes in the presence of weak acid preservatives and hydrochloric acid.

    abstract::This study addresses a major issue in microbial food safety, the elucidation of correlations between acid stress and changes in membrane fluidity of the pathogen Listeria monocytogenes. In order to assess the possible role that membrane fluidity changes play in L. monocytogenes tolerance to antimicrobial acids (acetic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00152

    authors: Diakogiannis I,Berberi A,Siapi E,Arkoudi-Vafea A,Giannopoulou L,Mastronicolis SK

    更新日期:2013-06-14 00:00:00

  • Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil.

    abstract::The human T-lymphotropic virus (HTLV) is part of the group of retroviruses that share similar routes of transmission to the human immunodeficiency virus (HIV). Coinfection of these viruses can affect the clinical course of both infections, and reports have shown a quicker progression to AIDS and the development of HIV...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.572381

    authors: Alencar SP,Souza MC,Fonseca RRS,Menezes CR,Azevedo VN,Ribeiro ALR,Lima SS,Laurentino RV,Barbosa MDAAP,Freitas FB,Oliveira-Filho AB,Machado LFA

    更新日期:2020-10-22 00:00:00

  • The haloarchaeal MCM proteins: bioinformatic analysis and targeted mutagenesis of the β7-β8 and β9-β10 hairpin loops and conserved zinc binding domain cysteines.

    abstract::The hexameric MCM complex is the catalytic core of the replicative helicase in eukaryotic and archaeal cells. Here we describe the first in vivo analysis of archaeal MCM protein structure and function relationships using the genetically tractable haloarchaeon Haloferax volcanii as a model system. Hfx. volcanii encodes...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00123

    authors: Kristensen TP,Maria Cherian R,Gray FC,MacNeill SA

    更新日期:2014-03-26 00:00:00

  • Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives.

    abstract::Arid regions show relatively fewer species in comparison to better-watered biomes, but the competition for the few nutrients is very distinct. Here, in total 373 bacterial strains were isolated from rhizospheric soils obtained from three different sampling sites in Tunisia. Their potential for the production of antimi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02742

    authors: Nasfi Z,Busch H,Kehraus S,Linares-Otoya L,König GM,Schäberle TF,Bachoual R

    更新日期:2018-11-13 00:00:00

  • Fungal Diversity in Tomato Rhizosphere Soil under Conventional and Desert Farming Systems.

    abstract::This study examined fungal diversity and composition in conventional (CM) and desert farming (DE) systems in Oman. Fungal diversity in the rhizosphere of tomato was assessed using 454-pyrosequencing and culture-based techniques. Both techniques produced variable results in terms of fungal diversity, with 25% of the fu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01462

    authors: Kazerooni EA,Maharachchikumbura SSN,Rethinasamy V,Al-Mahrouqi H,Al-Sadi AM

    更新日期:2017-08-02 00:00:00