A New Rabbit-Skin Model to Evaluate Protective Efficacy of Tuberculosis Vaccines.

Abstract:

:Background: BCG protection is suboptimal and there is significant interest to develop new tuberculosis (TB) vaccines. However, there are significant limitations of the current vaccine evaluation systems in the mouse model. Here, we developed a BCG-challenge rabbit skin model as a new way to evaluate the protective efficacy of selected TB subunit vaccine candidates. Methods: Rabbits were immunized with subunit vaccines, including EAMM (ESAT6-Ag85B-MPT64<190-198>-Mtb8.4), MH (Mtb10.4-HspX), and LT70 (ESAT6-Ag85B-MPT64<190-198>-Mtb8.4-Rv2626c) three times subcutaneously every 3-weeks and challenged with the attenuated Mycobacterium bovis BCG intradermally 6-weeks after last immunization. The immune response induced by the vaccine candidates was measured, the histopathology induced by the BCG challenge was studied, and the number of bacilli in the liquefied caseum was determined. Results: The subunit vaccines generated high antigen-specific IgG antibodies and fastened the liquefaction and healing process, and significantly reduced the viable BCG load. The subunit vaccine LT70 and EAMM-MH reduced BCG bacterial load in comparison to proteins EAMM, MH, Rv2626c, and also BCG itself. The Koch phenomena induced by the LT70 and combination of EAMM-MH were the same as that produced by BCG itself and were more rapid than those induced by the other proteins and the saline controls. Conclusions: The subunit vaccines LT70 and the combination of EAMM-MH showed promising protective efficacy as expected in the rabbit skin model, which can serve as a visual and convenient new model for evaluating TB vaccines.

journal_name

Front Microbiol

authors

Chen H,Liu X,Ma X,Wang Q,Yang G,Niu H,Li S,He B,He S,Dannenberg AM Jr,Zhu B,Zhang Y

doi

10.3389/fmicb.2017.00842

subject

Has Abstract

pub_date

2017-05-17 00:00:00

pages

842

issn

1664-302X

journal_volume

8

pub_type

杂志文章
  • Corrigendum: Antifungal Potential of the Skin Microbiota of Hibernating Big Brown Bats (Eptesicus fuscus) Infected With the Causal Agent of White-Nose Syndrome.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2020.01776.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2020.588889

    authors: Lemieux-Labonté V,Dorville NAS,Willis CKR,Lapointe FJ

    更新日期:2020-10-26 00:00:00

  • An Update on the Evolution of Glucosyltransferase (Gtf) Genes in Streptococcus.

    abstract::In many caries-promoting Streptococcus species, glucosyltransferases (Gtfs) are recognized as key enzymes contributing to the modification of biofilm structures, disruption of homeostasis of healthy microbiota community and induction of caries development. It is therefore of great interest to investigate how Gtf genes...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02979

    authors: Xu RR,Yang WD,Niu KX,Wang B,Wang WM

    更新日期:2018-12-04 00:00:00

  • Draft Genomic Analysis of an Avian Multidrug Resistant Morganella morganii Isolate Carrying qnrD1.

    abstract::Morganella morganii is a commensal bacterium and opportunistic pathogen often present in the gut of humans and animals. We report the 4.3 Mbp draft genome sequence of a M. morganii isolated in association with an Escherichia coli from broilers in Portugal that showed macroscopic lesions consistent with colisepticemia....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01660

    authors: Jones-Dias D,Clemente L,Moura IB,Sampaio DA,Albuquerque T,Vieira L,Manageiro V,Caniça M

    更新日期:2016-10-25 00:00:00

  • Biophysical methods for the study of microbial surfaces.

    abstract::The challenge in studying the surface architecture of different microbial pathogens is to integrate the most current biochemical, spectroscopic, microscopic, and processing techniques. Individually these methods have insufficient sensitivity to reveal complex structures, such as branched, large, viscous polymers with ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00207

    authors: Frases S,Viana NB,Casadevall A

    更新日期:2011-10-10 00:00:00

  • Production of the Plant Hormone Auxin by Salmonella and Its Role in the Interactions with Plants and Animals.

    abstract::The ability of human enteric pathogens to colonize plants and use them as alternate hosts is now well established. Salmonella, similarly to phytobacteria, appears to be capable of producing the plant hormone auxin via an indole-3-pyruvate decarboxylase (IpdC), a key enzyme of the IPyA pathway. A deletion of the Salmon...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02668

    authors: Cox CE,Brandl MT,de Moraes MH,Gunasekera S,Teplitski M

    更新日期:2018-01-12 00:00:00

  • Sterilization of liquid foods by pulsed electric fields-an innovative ultra-high temperature process.

    abstract::The intention of this study was to investigate the inactivation of endospores by a combined thermal and pulsed electric field (PEF) treatment. Therefore, self-cultivated spores of Bacillus subtilis and commercial Geobacillus stearothermophilus spores with certified heat resistance were utilized. Spores of both strains...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00400

    authors: Reineke K,Schottroff F,Meneses N,Knorr D

    更新日期:2015-05-06 00:00:00

  • oriC-encoded instructions for the initiation of bacterial chromosome replication.

    abstract::Replication of the bacterial chromosome initiates at a single origin of replication that is called oriC. This occurs via the concerted action of numerous proteins, including DnaA, which acts as an initiator. The origin sequences vary across species, but all bacterial oriCs contain the information necessary to guide as...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00735

    authors: Wolański M,Donczew R,Zawilak-Pawlik A,Zakrzewska-Czerwińska J

    更新日期:2015-01-06 00:00:00

  • Subgingival microbiota in health compared to periodontitis and the influence of smoking.

    abstract::The etiology of periodontitis has traditionally been associated to a consortium of three bacterial species-the so-called "red-complex" of periodontal disease-which has been the target for most diagnostic and therapeutic strategies. However, other species have also been found to correlate with disease severity. In addi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00119

    authors: Camelo-Castillo AJ,Mira A,Pico A,Nibali L,Henderson B,Donos N,Tomás I

    更新日期:2015-02-24 00:00:00

  • The Rhizosphere Microbiome of Mikania micrantha Provides Insight Into Adaptation and Invasion.

    abstract::Mikania micrantha is a noxious invasive plant causing enormous economic losses and ecological damage. Soil microbiome plays an important role in the invasion process of M. micrantha, while little is known about its rhizosphere microbiome composition and function. In this study, we identified the distinct rhizosphere m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01462

    authors: Yin L,Liu B,Wang H,Zhang Y,Wang S,Jiang F,Ren Y,Liu H,Liu C,Wan F,Wang H,Qian W,Fan W

    更新日期:2020-07-07 00:00:00

  • Recombination Does Not Hinder Formation or Detection of Ecological Species of Synechococcus Inhabiting a Hot Spring Cyanobacterial Mat.

    abstract::Recent studies of bacterial speciation have claimed to support the biological species concept-that reduced recombination is required for bacterial populations to diverge into species. This conclusion has been reached from the discovery that ecologically distinct clades show lower rates of recombination than that which...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01540

    authors: Melendrez MC,Becraft ED,Wood JM,Olsen MT,Bryant DA,Heidelberg JF,Rusch DB,Cohan FM,Ward DM

    更新日期:2016-01-14 00:00:00

  • Detection of Antimicrobial Resistance Genes in the Milk Production Environment: Impact of Host DNA and Sequencing Depth.

    abstract::Over the past decades, antimicrobial resistance (AMR) has been recognized as one of the most serious threats to public health. Although originally considered a problem to human health, the emerging crisis of AMR requires a "One Health" approach, considering human, animal, and environmental reservoirs. In this regard, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01983

    authors: Rubiola S,Chiesa F,Dalmasso A,Di Ciccio P,Civera T

    更新日期:2020-08-26 00:00:00

  • Lactic Acid Treatment of Cereals and Dietary Phytase Modified Fecal Microbiome Composition Without Affecting Expression of Virulence Factor Genes in Growing Pigs.

    abstract::Besides the major nutrients, phosphorus (P) is an essential mineral for both the host animal and the porcine gut microbiota. Different strategies including phytase supplementation and more recently lactic acid (LA) are used to enhance the P availability from cereals in pig diets; however, their impact on the gut micro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02345

    authors: Klinsoda J,Vötterl J,Zebeli Q,Metzler-Zebeli BU

    更新日期:2019-10-15 00:00:00

  • In vitro Determination of Extracellular Proteins from Xylella fastidiosa.

    abstract::The phytopathogen Xylella fastidiosa causes economic losses in important agricultural crops. Xylem vessel occlusion caused by biofilm formation is the major mechanism underlying the pathogenicity of distinct strains of X. fastidiosa. Here, we provide a detailed in vitro characterization of the extracellular proteins o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.02090

    authors: Mendes JS,Santiago AS,Toledo MA,Horta MA,de Souza AA,Tasic L,de Souza AP

    更新日期:2016-12-26 00:00:00

  • PVP-SVM: Sequence-Based Prediction of Phage Virion Proteins Using a Support Vector Machine.

    abstract::Accurately identifying bacteriophage virion proteins from uncharacterized sequences is important to understand interactions between the phage and its host bacteria in order to develop new antibacterial drugs. However, identification of such proteins using experimental techniques is expensive and often time consuming; ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00476

    authors: Manavalan B,Shin TH,Lee G

    更新日期:2018-03-16 00:00:00

  • Continuous Adaptive Evolution of a Fast-Growing Corynebacterium glutamicum Strain Independent of Protocatechuate.

    abstract::Corynebacterium glutamicum is a commonly applied host for the industrial production of amino acids. While valued for its robustness, it is somewhat inferior to competing strains such as Escherichia coli because of the relatively low growth rate of 0.40 h-1 in synthetic, industrial media. Accordingly, adaptive laborato...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01648

    authors: Graf M,Haas T,Müller F,Buchmann A,Harm-Bekbenbetova J,Freund A,Nieß A,Persicke M,Kalinowski J,Blombach B,Takors R

    更新日期:2019-08-06 00:00:00

  • 5-Aminosalicylic Acid Alters the Gut Bacterial Microbiota in Patients With Ulcerative Colitis.

    abstract::Background: The aim of this study was to clarify the effect of 5-aminosalicylic acid (5-ASA) treatment on gut bacterial microbiota in patients with ulcerative colitis (UC). Methods: A total of 57 UC patients, including 20 untreated and 37 5-ASA-treated, were recruited into an exploration cohort. We endoscopically coll...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01274

    authors: Xu J,Chen N,Wu Z,Song Y,Zhang Y,Wu N,Zhang F,Ren X,Liu Y

    更新日期:2018-06-13 00:00:00

  • Comparison of methods for the enumeration of enterohemorrhagic Escherichia coli from veal hides and carcasses.

    abstract::The increased association of enterohemorrhagic Escherichia coli (EHEC) with veal calves has led the United States Department of Agriculture Food Safety and Inspection Service to report results of veal meat contaminated with the Top 7 serogroups separately from beef cattle. However, detection methods that can also prov...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01062

    authors: Luedtke BE,Bosilevac JM

    更新日期:2015-09-29 00:00:00

  • Efficient Biofilm-Based Fermentation Strategies for L-Threonine Production by Escherichia coli.

    abstract::Biofilms provide cells favorable growth conditions, which have been exploited in industrial biotechnological processes. However, industrial application of the biofilm has not yet been reported in Escherichia coli, one of the most important platform strains, though the biofilm has been extensively studied for pathogeni...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01773

    authors: Chen T,Liu N,Ren P,Xi X,Yang L,Sun W,Yu B,Ying H,Ouyang P,Liu D,Chen Y

    更新日期:2019-08-02 00:00:00

  • Distinctive Gene Expression Profiles and Effectors Consistent With Host Specificity in Two Formae Speciales of Marssonina brunnea.

    abstract::The knowledge on the host specificity of a pathogen underlying an interaction is becoming an urgent necessity for global warming. In this study, the gene expression profiles and the roles of effectors in host specificity were integrally characterized with two formae speciales, multigermtubi and monogermtubi, of a hemi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00276

    authors: Ren F,Yan DH,Wu G,Sun X,Song X,Li R

    更新日期:2020-03-10 00:00:00

  • Extracellular Trap Formation in Response to Trypanosoma cruzi Infection in Granulocytes Isolated From Dogs and Common Opossums, Natural Reservoir Hosts.

    abstract::Granulocytes mediate the first line of defense against infectious diseases in humans as well as animals and they are well known as multitasking cells. They can mediate antimicrobial activity by different strategies depending on the pathogen they encounter. Besides phagocytosis, a key strategy against extracellular pat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00966

    authors: de Buhr N,Bonilla MC,Jimenez-Soto M,von Köckritz-Blickwede M,Dolz G

    更新日期:2018-05-15 00:00:00

  • Combined Metabarcoding and Co-occurrence Network Analysis to Profile the Bacterial, Fungal and Fusarium Communities and Their Interactions in Maize Stalks.

    abstract::Fusarium Head Blight (FHB) is one of the most devastating diseases of cereals worldwide, threatening both crop production by affecting cereal grain development, and human and animal health by contaminating grains with mycotoxins. Despite that maize residues constitute the primary source of inoculum for Fusarium pathog...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00261

    authors: Cobo-Díaz JF,Baroncelli R,Le Floch G,Picot A

    更新日期:2019-02-18 00:00:00

  • Predicting the Responses of Soil Nitrite-Oxidizers to Multi-Factorial Global Change: A Trait-Based Approach.

    abstract::Soil microbial diversity is huge and a few grams of soil contain more bacterial taxa than there are bird species on Earth. This high diversity often makes predicting the responses of soil bacteria to environmental change intractable and restricts our capacity to predict the responses of soil functions to global change...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00628

    authors: Le Roux X,Bouskill NJ,Niboyet A,Barthes L,Dijkstra P,Field CB,Hungate BA,Lerondelle C,Pommier T,Tang J,Terada A,Tourna M,Poly F

    更新日期:2016-05-17 00:00:00

  • Global Transcriptomic Changes Induced by Infection of Cucumber (Cucumis sativus L.) with Mild and Severe Variants of Hop Stunt Viroid.

    abstract::Fifteen years after transfer to hops, hop stunt viroid-grapevine (HSVd-g) was replaced by HSVd-hop (HSVd-h), a sequence variant that contains changes at five different positions. HSVd-g54 is a laboratory mutant derived from HSVd-g that differs from its progenitor by a single G to A substitution at position 54. While i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02427

    authors: Xia C,Li S,Hou W,Fan Z,Xiao H,Lu M,Sano T,Zhang Z

    更新日期:2017-12-12 00:00:00

  • Diversity and Composition of Airborne Fungal Community Associated with Particulate Matters in Beijing during Haze and Non-haze Days.

    abstract::To assess the diversity and composition of airborne fungi associated with particulate matters (PMs) in Beijing, China, a total of 81 PM samples were collected, which were derived from PM2.5, PM10 fractions, and total suspended particles during haze and non-haze days. The airborne fungal community in these samples was ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00487

    authors: Yan D,Zhang T,Su J,Zhao LL,Wang H,Fang XM,Zhang YQ,Liu HY,Yu LY

    更新日期:2016-04-14 00:00:00

  • A PAS domain-containing regulator controls flagella-flagella interactions in Campylobacter jejuni.

    abstract::The bipolar flagella of the foodborne bacterial pathogen Campylobacter jejuni confer motility, which is essential for virulence. The flagella of C. jejuni are post-translationally modified, but how this process is controlled is not well understood. In this work, we have identified a novel PAS-domain containing regulat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00770

    authors: Reuter M,Periago PM,Mulholland F,Brown HL,van Vliet AH

    更新日期:2015-07-30 00:00:00

  • Fungal diversity in grape must and wine fermentation assessed by massive sequencing, quantitative PCR and DGGE.

    abstract::The diversity of fungi in grape must and during wine fermentation was investigated in this study by culture-dependent and culture-independent techniques. Carignan and Grenache grapes were harvested from three vineyards in the Priorat region (Spain) in 2012, and nine samples were selected from the grape must after crus...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01156

    authors: Wang C,García-Fernández D,Mas A,Esteve-Zarzoso B

    更新日期:2015-10-23 00:00:00

  • Diversity of Mobile Genetic Elements in the Mitogenomes of Closely Related Fusarium culmorum and F. graminearum sensu stricto Strains and Its Implication for Diagnostic Purposes.

    abstract::Much of the mitogenome variation observed in fungal lineages seems driven by mobile genetic elements (MGEs), which have invaded their genomes throughout evolution. The variation in the distribution and nucleotide diversity of these elements appears to be the main distinction between different fungal taxa, making them ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01002

    authors: Kulik T,Brankovics B,van Diepeningen AD,Bilska K,Żelechowski M,Myszczyński K,Molcan T,Stakheev A,Stenglein S,Beyer M,Pasquali M,Sawicki J,Wyrȩbek J,Baturo-Cieśniewska A

    更新日期:2020-05-25 00:00:00

  • Evidence of New Endemic Clusters of Human T-Cell Leukemia Virus (HTLV) Infection in Bahia, Brazil.

    abstract:Background:Salvador, Bahia (northeastern Brazil), has been identified as the epicenter of Human T-cell leukemia virus Human T-cell leukemia virus (HTLV) type 1 infection in the country. This study aims to estimate the rate of HTLV infection and the geographical distribution of this virus in this state. Methods:All HTL...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01002

    authors: Pereira FM,de Almeida MDCC,Santos FLN,Carreiro RP,Regis-Silva CG,Galvão-Castro B,Grassi MFR

    更新日期:2019-05-14 00:00:00

  • Conductive Fe3O4 Nanoparticles Accelerate Syntrophic Methane Production from Butyrate Oxidation in Two Different Lake Sediments.

    abstract::Syntrophic methanogenesis is an essential link in the global carbon cycle and a key bioprocess for the disposal of organic waste and production of biogas. Recent studies suggest direct interspecies electron transfer (DIET) is involved in electron exchange in methanogenesis occurring in paddy soils, anaerobic digesters...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01316

    authors: Zhang J,Lu Y

    更新日期:2016-08-22 00:00:00

  • GPD1 and ADH3 Natural Variants Underlie Glycerol Yield Differences in Wine Fermentation.

    abstract::Glycerol is one of the most important by-products of alcohol fermentation, and depending on its concentration it can contribute to wine flavor intensity and aroma volatility. Here, we evaluated the potential of utilizing the natural genetic variation of non-coding regions in budding yeast to identify allelic variants ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01460

    authors: Tapia SM,Cuevas M,Abarca V,Delgado V,Rojas V,García V,Brice C,Martínez C,Salinas F,Larrondo LF,Cubillos FA

    更新日期:2018-07-03 00:00:00