Resistance to Ditylenchus destructor Infection in Sweet Potato by the Expression of Small Interfering RNAs Targeting unc-15, a Movement-Related Gene.

Abstract:

:Stem nematode (Ditylenchus destructor) is one of most serious diseases that limit the productivity and quality of sweet potato (Ipomoea batatas), a root crop with worldwide importance for food security and nutrition improvement. Hence, there is a global demand for developing sweet potato varieties that are resistant to the disease. In this study, we have investigated the interference of stem nematode infectivity by the expression of small interfering RNAs (siRNAs) in transgenic sweet potato that are homologous to the unc-15 gene, which affects the muscle protein paramyosin of the pathogen. The production of double-stranded RNAs and siRNAs in transgenic lines with a single transgene integration event was verified by Northern blot analysis. The expression of unc-15 was reduced dramatically in stem nematodes collected from the inoculated storage roots of transgenic plants, and the infection areas of their storage roots were dramatically smaller than that of wild-type (WT). Compared with the WT, the transgenic plants showed increased yield in the stem nematode-infested field. Our results demonstrate that the expression of siRNAs targeting the unc-15 gene of D. destructor is an effective approach in improving stem nematode resistance in sweet potato, in adjunct with the global integrated pest management programs.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Fan W,Wei Z,Zhang M,Ma P,Liu G,Zheng J,Guo X,Zhang P

doi

10.1094/PHYTO-04-15-0087-R

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

1458-65

issue

11

eissn

0031-949X

issn

1943-7684

journal_volume

105

pub_type

杂志文章
  • Zoospore density-dependent behaviors of Phytophthora nicotianae are autoregulated by extracellular products.

    abstract::Phytophthora species are destructive fungus-like plant pathogens that use asexual single-celled flagellate zoospores for dispersal and plant infection. Many of the zoospore behaviors are density-dependent although the underlying mechanisms are poorly understood. Here, we use P. nicotianae as a model and demonstrate au...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-100-7-0632

    authors: Kong P,Hong C

    更新日期:2010-07-01 00:00:00

  • The Role of a Host-Induced Arginase of Xanthomonas oryzae pv. oryzae in Promoting Virulence on Rice.

    abstract::The plant bacterial pathogen Xanthomonas oryzae pv. oryzae causes bacterial blight of rice, which is one of the most destructive rice diseases prevalent in Asia and parts of Africa. Despite many years of research, how X. oryzae pv. oryzae causes bacterial blight of rice is still not completely understood. Here, we sho...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-02-19-0058-R

    authors: Zhang Y,Wu G,Palmer I,Wang B,Qian G,Fu ZQ,Liu F

    更新日期:2019-11-01 00:00:00

  • Pathogenicity and In Planta Mycotoxin Accumulation Among Members of the Fusarium graminearum Species Complex on Wheat and Rice.

    abstract::ABSTRACT Fusarium head blight (FHB), or scab, is a destructive disease of small grains caused by members of the Fusarium graminearum species complex, comprised of at least nine distinct, cryptic species. Members of this complex are known to produce mycotoxins including the trichothecenes deoxynivalenol (DON) along wit...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-1397

    authors: Goswami RS,Kistler HC

    更新日期:2005-12-01 00:00:00

  • Sensitivity of Mycosphaerella fijiensis, Causal Agent of Black Sigatoka of Banana, to Propiconazole.

    abstract::ABSTRACT One hundred monoascosporic isolates of Mycosphaerella fijiensis were collected in February and November 1994 from each of two banana (Musa spp.) plantations in Costa Rica. Locations at San Pablo and Coopecariari had been sprayed with propiconazole for the past 7 years to control black Sigatoka. One hundred mo...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1997.87.1.96

    authors: Romero RA,Sutton TB

    更新日期:1997-01-01 00:00:00

  • Comparative Genomics to Develop a Specific Multiplex PCR Assay for Detection of Clavibacter michiganensis.

    abstract::Clavibacter michiganensis is a Gram-positive bacterial pathogen that proliferates in the xylem vessels of tomato, causing bacterial wilt and canker symptoms. Accurate detection is a crucial step in confirming outbreaks of bacterial canker and developing management strategies. A major problem with existing detection me...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-10-19-0405-R

    authors: Thapa SP,O'Leary M,Jacques MA,Gilbertson RL,Coaker G

    更新日期:2020-03-01 00:00:00

  • Soybean sudden death syndrome species diversity within north and South america revealed by multilocus genotyping.

    abstract::Sudden death syndrome (SDS) of soybean has become a serious constraint to the production of this crop in North and South America. Phenotypic and multilocus molecular phylogenetic analyses, as well as pathogenicity experiments, have demonstrated that four morphologically and phylogenetically distinct fusaria can induce...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-100-1-0058

    authors: O'Donnell K,Sink S,Scandiani MM,Luque A,Colletto A,Biasoli M,Lenzi L,Salas G,González V,Ploper LD,Formento N,Pioli RN,Aoki T,Yang XB,Sarver BA

    更新日期:2010-01-01 00:00:00

  • Genes for parasite-specific, nonhost resistance in populus.

    abstract::ABSTRACT Nonhost resistance is hypothesized to protect plants in a nonspecific manner. For highly specialized parasites, this hypothesis applies not only to distantly related plants but also to resistant congeners of the host species. Congeners of Populus spp. were hybridized to create two interspecific hybrid poplar ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-0779

    authors: Newcombe G

    更新日期:2005-07-01 00:00:00

  • Distinguishing Galactomyces citri-aurantii from G. geotrichum and characterizing population structure of the two postharvest sour rot pathogens of fruit crops in California.

    abstract::A growth assay in lemon juice and polymerase chain reaction amplifications using newly designed species-specific primers from endopolygalacturonase and β-tubulin genes rapidly differentiated isolates of the morphologically similar fruit sour rot pathogens Galactomyces citriaurantii and G. geotrichum. Isolates of both ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-05-11-0156

    authors: McKay AH,Förster H,Adaskaveg JE

    更新日期:2012-05-01 00:00:00

  • Natural Hybrids of Phytophthora nicotianae and Phytophthora cactorum Demonstrated by Isozyme Analysis and Random Amplified Polymorphic DNA.

    abstract::ABSTRACT Three similar isolates of Phytophthora (Phytophthora sp-h) were obtained from diseased Spathiphyllum and Primula plants. Cultural characteristics did not fit any known description of Phytophthora species. The Phytophthora sp-h isolates are papillate, are homothallic, possess 80 to 86% amphigynous antheridia, ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1998.88.9.922

    authors: Man In 't Veld WA,Veenbaas-Rijks WJ,Ilieva E,de Cock AW,Bonants PJ,Pieters R

    更新日期:1998-09-01 00:00:00

  • Epicuticular Wax and White Pine Blister Rust Resistance in Resistant and Susceptible Selections of Eastern White Pine (Pinus strobus).

    abstract::ABSTRACT Epicuticular wax on needles was evaluated for its influence on Cronartium ribicola infection of resistant and susceptible selections of Pinus strobus. Environmental scanning electron microscopy comparisons revealed that needles from a resistant selection of eastern white pine, P327, had a significantly higher...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-96-0171

    authors: Smith JA,Blanchette RA,Burnes TA,Gillman JH,David AJ

    更新日期:2006-02-01 00:00:00

  • Genetic Variability of Grapevine Pinot gris virus and Its Association with Grapevine Leaf Mottling and Deformation.

    abstract::The role of Grapevine Pinot gris virus (GPGV) in the etiology of grapevine leaf mottling and deformation was investigated by biological and molecular assays. A survey on different cultivars from the Trentino Region in Italy showed a widespread distribution of GPGV, which was associated with symptomatic (79%) but also ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-09-14-0241-R

    authors: Saldarelli P,Giampetruzzi A,Morelli M,Malossini U,Pirolo C,Bianchedi P,Gualandri V

    更新日期:2015-04-01 00:00:00

  • Influence of crop management on take-all development and disease cycles on winter wheat.

    abstract::ABSTRACT Wheat was assessed at four crop growth stages for take-all (Gaeumannomyces graminis var. tritici) in a series of field trials that studied the effects of five wheat management practices: sowing date, plant density, nitrogen fertilizer dose and form, and removal/burial of cereal straw. An equation expressing d...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1997.87.1.26

    authors: Colbach N,Lucas P,Meynard JM

    更新日期:1997-01-01 00:00:00

  • The Effect of Resin and Monoterpenes on Spore Germination and Growth in Fusarium circinatum.

    abstract::Resin obtained from Pinus radiata and five monoterpene components of resin (limonene, α-pinene, β-pinene, camphene, and myrcene) were tested to determine their effects on mycelial growth and germination and survival of spores of Fusarium circinatum, the cause of pitch canker in pine, and F. temperatum, which is interf...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-02-14-0027-R

    authors: Slinski SL,Zakharov F,Gordon TR

    更新日期:2015-01-01 00:00:00

  • Mechanism of Action of the Benzimidazole Fungicide on Fusarium graminearum: Interfering with Polymerization of Monomeric Tubulin But Not Polymerized Microtubule.

    abstract::Tubulins are the proposed target of clinically relevant anticancer drugs, anthelmintic, and fungicide. β2-tubulin of the plant pathogen Fusarium graminearum was considered as the target of benzimidazole compounds by homology modeling in our previous work. In this study, α1-, α2-, and β2-tubulin of F. graminearum were ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-08-15-0186-R

    authors: Zhou Y,Xu J,Zhu Y,Duan Y,Zhou M

    更新日期:2016-08-01 00:00:00

  • Meta-analysis in plant pathology: synthesizing research results.

    abstract::ABSTRACT Meta-analysis is a set of statistical procedures for synthesizing research results from a number of different studies. An estimate of a statistical effect, such as the difference in disease severity for plants with or without a management treatment, is collected from each study along with a measure of the var...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.9.1013

    authors: Rosenberg MS,Garrett KA,Su Z,Bowden RL

    更新日期:2004-09-01 00:00:00

  • trans-Dominant Inhibition of Geminiviral DNA Replication by Bean Golden Mosaic Geminivirus rep Gene Mutants.

    abstract::ABSTRACT Geminiviruses are a group of single-stranded DNA viruses that cause major losses on a number of important crops throughout the world. Bean golden mosaic virus (BGMV) is a typical bipartite, whitefly-transmitted geminivirus that causes a severe disease on beans (Phaseolus vulgaris) in the Western Hemisphere. T...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.6.480

    authors: Hanson SF,Maxwell DP

    更新日期:1999-06-01 00:00:00

  • Callose Synthase Family Genes Involved in the Grapevine Defense Response to Downy Mildew Disease.

    abstract::The deposition of callose is a common plant defense response to intruding pathogens and part of the plant's innate immunity. In this study, eight grapevine callose synthase (CalS) genes were identified and characterized. To investigate biological function of CalS in grapevine against the infection of Plasmopara vitico...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-07-15-0166-R

    authors: Yu Y,Jiao L,Fu S,Yin L,Zhang Y,Lu J

    更新日期:2016-01-01 00:00:00

  • Vegetative Compatibility Groups in Colletotrichum coccodes, the Causal Agent of Black Dot on Potato.

    abstract::ABSTRACT Black dot of potato, caused by Colletotrichum coccodes, is a disease of growing economic importance, but the degree of genetic diversity and pathogenic differentiation among isolates is unknown. Using nitrate auxotrophic (Nit) mutants, we characterized vegetative compatibility groups (VCG) diversity for C. co...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2002.92.8.827

    authors: Nitzan N,Hazanovsky M,Tal M,Tsror Lahkim L

    更新日期:2002-08-01 00:00:00

  • Effects of Varying Environmental Conditions on Biological Control of Fusarium Wilt of Tomato by Nonpathogenic Fusarium spp.

    abstract::ABSTRACT The influence of varying environmental and cropping conditions including temperature, light, soil type, pathogen isolate and race, and cultivar of tomato on biological control of Fusarium wilt of tomato by isolates of nonpathogenic Fusarium oxysporum (CS-20 and CS-24) and F. solani (CS-1) was evaluated in gre...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2002.92.11.1160

    authors: Larkin RP,Fravel DR

    更新日期:2002-11-01 00:00:00

  • Potato Virus Y (PVY) Isolates from Solanum betaceum Represent Three Novel Recombinants Within the PVYN Strain Group and Are Unable to Systemically Spread in Potato.

    abstract::Tamarillo, or tree tomato (Solanum betaceum), is a perennial small tree or shrub species cultivated in subtropical areas for fresh fruit and juice production. In Ecuador, tamarillo orchards are affected by several viruses, with one previously identified as potato virus Y (PVY); however, the specific strain composition...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-04-20-0111-R

    authors: Green KJ,Funke CN,Chojnacky J,Alvarez-Quinto RA,Ochoa JB,Quito-Avila DF,Karasev AV

    更新日期:2020-09-01 00:00:00

  • Reproductive Ability of Hybrids of Striga aspera and Striga hermonthica.

    abstract::ABSTRACT Striga aspera and S. hermonthica are sympatric in Africa. Each may serve as virulent gene reservoirs for the other if they hybridize and their hybrids are virulent and fertile. Intraspecific and interspecific crosses were made within and between the species, and reproductive success was determined. Freshly ha...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1998.88.6.563

    authors: Aigbokhan EI,Berner DK,Musselman LJ

    更新日期:1998-06-01 00:00:00

  • A Double Mutation in the Conserved Motifs of the Helper Component Protease of Papaya Leaf Distortion Mosaic Virus for the Generation of a Cross-Protective Attenuated Strain.

    abstract::Potyviral helper component protease (HC-Pro), as a major determinant of symptom expression in susceptible plants, is a likely target candidate in the production of attenuated strains for cross-protection. In this study, single or double mutations of Lys (K) to Glu (E) in the Lys-Ile-Thr-Cys motif and Arg (R) to Ile (I...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-09-19-0328-R

    authors: Tuo D,Zhou P,Zhao G,Yan P,Tan D,Li X,Shen W

    更新日期:2020-01-01 00:00:00

  • Functional analysis of Pid3-A4, an ortholog of rice blast resistance gene Pid3 revealed by allele mining in common wild rice.

    abstract::The rice blast resistance gene Pid3 encodes a nucleotide-binding-site leucine-rich repeat (NBS-LRR) protein. This gene was cloned from the rice 'Digu' (indica) by performing a genome-wide comparison of the NBS-LRR gene family between two genome-sequenced varieties, '9311' (indica) and 'Nipponbare' (japonica). In this ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-10-12-0260-R

    authors: Lv Q,Xu X,Shang J,Jiang G,Pang Z,Zhou Z,Wang J,Liu Y,Li T,Li X,Xu J,Cheng Z,Zhao X,Li S,Zhu L

    更新日期:2013-06-01 00:00:00

  • Ultrastructural and Cytochemical Aspects of the Biological Control of Botrytis cinerea by Candida saitoana in Apple Fruit.

    abstract::ABSTRACT Biocontrol activity of Candida saitoana and its interaction with Botrytis cinerea in apple wounds were investigated. When cultured together, yeast attached to Botrytis sp. hyphal walls. In wounded apple tissue, C. saitoana restricted the proliferation of B. cinerea, multiplied, and suppressed disease caused b...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1998.88.4.282

    authors: El-Ghaouth A,Wilson CL,Wisniewski M

    更新日期:1998-04-01 00:00:00

  • Coupling Spore Traps and Quantitative PCR Assays for Detection of the Downy Mildew Pathogens of Spinach (Peronospora effusa) and Beet (P. schachtii).

    abstract::Downy mildew of spinach (Spinacia oleracea), caused by Peronospora effusa, is a production constraint on production worldwide, including in California, where the majority of U.S. spinach is grown. The aim of this study was to develop a real-time quantitative polymerase chain reaction (qPCR) assay for detection of airb...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-02-14-0054-R

    authors: Klosterman SJ,Anchieta A,McRoberts N,Koike ST,Subbarao KV,Voglmayr H,Choi YJ,Thines M,Martin FN

    更新日期:2014-12-01 00:00:00

  • Identification and Characterization of Menadione and Benzethonium Chloride as Potential Treatments of Pierce's Disease of Grapevines.

    abstract::Xylella fastidiosa infects a wide range of plant hosts and causes Pierce's disease (PD) of grapevines. The type 1 multidrug resistance (MDR) efflux system is essential for pathogenicity and survival of bacterial pathogens in planta. X. fastidiosa, with a single MDR system, is significantly more vulnerable to inhibitio...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-07-18-0244-FI

    authors: Zhang S,Jain M,Fleites LA,Rayside PA,Gabriel DW

    更新日期:2019-02-01 00:00:00

  • Extending the fungal host range of a partitivirus and a mycoreovirus from Rosellinia necatrix by inoculation of protoplasts with virus particles.

    abstract::The potential host range of mycoviruses is poorly understood because of the lack of suitable inoculation methods. Recently, successful transfection has been reported for somatically incompatible fungal isolates with purified virus particles of two mycoviruses, the partitivirus RnPV1-W8 (RnPV1) and the mycoreovirus RnM...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-100-9-0922

    authors: Kanematsu S,Sasaki A,Onoue M,Oikawa Y,Ito T

    更新日期:2010-09-01 00:00:00

  • Spiroplasma citri Spiralin Acts In Vitro as a Lectin Binding to Glycoproteins from Its Insect Vector Circulifer haematoceps.

    abstract::ABSTRACT In order to understand the molecular mechanisms underlying transmission of Spiroplasma citri by the leafhopper Circulifer haematoceps, we screened leafhopper proteins as putative S. citri-binding molecules using a spiroplasma overlay assay of protein blots (Far-western assay). Insect proteins were separated b...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-0541

    authors: Killiny N,Castroviejo M,Saillard C

    更新日期:2005-05-01 00:00:00

  • Improved Control of Postharvest Decay of Pears by the Combination of Candida sake (CPA-1) and Ammonium Molybdate.

    abstract::ABSTRACT The potential enhancement of Candida sake (CPA-1) by ammonium molybdate to control blue and gray mold caused by Penicillium expansum and Botrytis cinerea, respectively, on Blanquilla pears was investigated. In laboratory trials, improved control of blue and gray molds was obtained with the application of ammo...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2002.92.3.281

    authors: Nunes C,Usall J,Teixidó N,Abadias M,Viñas I

    更新日期:2002-03-01 00:00:00

  • Effect of wetness duration and temperature on the development of anthracnose on selected almond tissues and comparison of cultivar susceptibility.

    abstract::Blossoms, leaves, fruit, and woody tissues of almond can be affected by anthracnose caused by Colletotrichum acutatum. Because the disease occurs throughout rainy spring seasons, the effect of temperature and wetness duration on disease development was evaluated in controlled studies. The lowest inoculum concentration...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-07-10-0193

    authors: Diéguez-Uribeondo J,Förster H,Adaskaveg JE

    更新日期:2011-08-01 00:00:00