Sensitivity of Mycosphaerella fijiensis, Causal Agent of Black Sigatoka of Banana, to Propiconazole.

Abstract:

:ABSTRACT One hundred monoascosporic isolates of Mycosphaerella fijiensis were collected in February and November 1994 from each of two banana (Musa spp.) plantations in Costa Rica. Locations at San Pablo and Coopecariari had been sprayed with propiconazole for the past 7 years to control black Sigatoka. One hundred monoascosporic isolates from a third location, San Carlos, with no history of fungicide use, also were tested for sensitivity to propiconazole. Fifty percent effective concentration (EC(50)) values were calculated for individual isolates by regressing the relative inhibition of colony growth against the natural logarithm of the fungicide concentration. In the February sample, the mean EC(50) values for San Pablo and Coopecariari populations were 0.06 and 0.05 mug a.i. ml(-1), respectively, which were not statistically different (P = 0.05). The mean EC(50) value of the population at San Carlos was 0.008 mug a.i. ml(-1), which was significantly lower (P = 0.001) than the mean EC(50) values obtained at San Pablo and Coopecariari. Frequency distributions of EC(50) values of isolates from the three populations collected in February showed that 80% of isolates from San Pablo and Coopecariari had EC(50) values greater than the highest EC(50) value from San Carlos, indicating a significant shift in reduced sensitivity to propiconazole. Isolates collected in November 1994, after eight treatments of propiconazole at San Pablo and Coopecariari, showed a significant increase in mean EC(50) values compared with the means observed in February. The high proportion of isolates with reduced sensitivity to propiconazole may account for the unsatisfactory control of black Sigatoka between 1992 and 1993 in the two banana plantations at San Pablo and Coopecariari.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Romero RA,Sutton TB

doi

10.1094/PHYTO.1997.87.1.96

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

96-100

issue

1

eissn

0031-949X

issn

1943-7684

journal_volume

87

pub_type

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