Identification and Characterization of Menadione and Benzethonium Chloride as Potential Treatments of Pierce's Disease of Grapevines.

Abstract:

:Xylella fastidiosa infects a wide range of plant hosts and causes Pierce's disease (PD) of grapevines. The type 1 multidrug resistance (MDR) efflux system is essential for pathogenicity and survival of bacterial pathogens in planta. X. fastidiosa, with a single MDR system, is significantly more vulnerable to inhibition by small-molecule treatments than most bacterial pathogens that typically carry redundant MDR systems. A high-throughput cell viability assay using a green fluorescent protein-marked strain of X. fastidiosa Temecula 1 was developed to screen two Prestwick combinatorial small-molecule libraries of drugs and phytochemicals (1,600 chemicals in total) approved by the Food and Drug Administration and European Medicines Agency for cell growth inhibition. The screens revealed 215 chemicals that inhibited bacterial growth by >50% at 50 µM concentrations. Seven chemicals proved to lyse X. fastidiosa cells at 25 µM, including four phytochemicals. Menadione (2-methyl-1,4-naphthoquinone, vitamin K) from the phytochemical library and benzethonium chloride (a topical disinfectant) from the chemical library both showed significant bactericidal activity against X. fastidiosa. Both menadione and benzethonium chloride foliar spray (15 and 5 mM, respectively) and soil drench (5 and 25 mM, respectively) treatments were equally effective in reducing PD symptoms by 54 to 59% and revealed that the effects of both chemical treatments became systemic. However, menadione was phytotoxic when applied as a foliar spray at effective concentrations, causing significant loss of photosynthetic capacity.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Zhang S,Jain M,Fleites LA,Rayside PA,Gabriel DW

doi

10.1094/PHYTO-07-18-0244-FI

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

233-239

issue

2

eissn

0031-949X

issn

1943-7684

journal_volume

109

pub_type

杂志文章
  • Septoria Leaf Spot of Banana: A Newly Discovered Disease Caused by Mycosphaerella eumusae (Anamorph Septoria eumusae).

    abstract::ABSTRACT A previously undescribed leaf spot disease of banana has been discovered in southern and Southeast Asia. The fungus identified as the causal agent of this leaf spot has a Mycosphaerella teleomorph stage and a Septoria anamorph stage. Isolation and reinoculation of the fungus to banana reproduced symptoms and ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2000.90.8.884

    authors: Carlier J,Zapater MF,Lapeyre F,Jones DR,Mourichon X

    更新日期:2000-08-01 00:00:00

  • Gentian mosaic virus: A New Species in the Genus Fabavirus.

    abstract::ABSTRACT A viral isolate, designated N-1 and obtained from a gentian (Gentiana scabra) plant that exhibited mosaic symptoms, was transmitted mechanically to nine plant species in six families. These plants are known as hosts of fabaviruses. The N-1 isolate was composed of isometric particles 30 nm in diameter and incl...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-0192

    authors: Kobayashi YO,Kobayashi A,Hagiwara K,Uga H,Mikoshiba Y,Naito T,Honda Y,Omura T

    更新日期:2005-02-01 00:00:00

  • Resurgence of Less-Studied Smut Fungi as Models of Phytopathogenesis in the Omics Age.

    abstract::The smut fungi form a large, diverse, and nonmonophyletic group of plant pathogens that have long served as both important pests of human agriculture and, also, as fertile organisms of scientific investigation. As modern techniques of molecular genetic analysis became available, many previously studied species that pr...

    journal_title:Phytopathology

    pub_type: 杂志文章,评审

    doi:10.1094/PHYTO-02-16-0075-RVW

    authors: Toh SS,Perlin MH

    更新日期:2016-11-01 00:00:00

  • Core Rot Development in Red Delicious Apples Is Affected by Susceptibility of the Seed Locule to Alternaria alternata Colonization.

    abstract::ABSTRACT Alternaria alternata is the causal organism of core rot decay symptoms in susceptible cv. Red Delicious but not in resistant cv. Golden Delicious. The two cultivars did not differ in natural colonization of the style and ovary during the first week after full bloom; colonization of the ovary in the susceptibl...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-11-1415

    authors: Niem J,Miyara I,Ettedgui Y,Reuveni M,Flaishman M,Prusky D

    更新日期:2007-11-01 00:00:00

  • Molecular mapping of rsp1, rsp2, and rsp3 genes conferring resistance to septoria speckled leaf blotch in barley.

    abstract::ABSTRACT Septoria speckled leaf blotch (SSLB) caused by Septoria passerinii is a common disease in barley. SSLB resistance genes Rsp1, Rsp2, and Rsp3 have previously been identified in the United States Department of Agriculture National Small Grains collection accessions CIho 14300, CIho 4780, and CIho 10644, respect...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-2-0155

    authors: Lee SH,Neate SM

    更新日期:2007-02-01 00:00:00

  • The Use of Green Fluorescent Protein-Tagged Recombinant Viruses to Test Lettuce mosaic virus Resistance in Lettuce.

    abstract::ABSTRACT Seed certification and the use of cultivars containing one of two, probably allelic, recessive genes, mo1(1) and mo1(2), are the principal control methods for Lettuce mosaic virus (LMV) in lettuce. Although for a few LMV isolates, mo1(2) confers resistance with most isolates, the genes mo1(1) or mo1(2) confer...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2002.92.2.169

    authors: Candresse T,Le Gall O,Maisonneuve B,German-Retana S,Redondo E

    更新日期:2002-02-01 00:00:00

  • Sensitivity of Mycosphaerella fijiensis, Causal Agent of Black Sigatoka of Banana, to Propiconazole.

    abstract::ABSTRACT One hundred monoascosporic isolates of Mycosphaerella fijiensis were collected in February and November 1994 from each of two banana (Musa spp.) plantations in Costa Rica. Locations at San Pablo and Coopecariari had been sprayed with propiconazole for the past 7 years to control black Sigatoka. One hundred mo...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1997.87.1.96

    authors: Romero RA,Sutton TB

    更新日期:1997-01-01 00:00:00

  • Populations of Nonpathogenic Fusarium oxysporum Associated with Roots of Four Plant Species Compared to Soilborne Populations.

    abstract::ABSTRACT The effect of the plant on the diversity of soilborne populations of Fusarium oxysporum was assessed after successive cultures of flax, melon, tomato, and wheat in separate samples of the same soil. Forty soil-borne isolates of F. oxysporum and forty root-colonizing isolates of each plant species were sampled...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1997.87.7.693

    authors: Edel V,Steinberg C,Gautheron N,Alabouvette C

    更新日期:1997-07-01 00:00:00

  • Molecular analyses of colletotrichum species from almond and other fruits.

    abstract::ABSTRACT Isolates of Colletotrichum spp. from almond, avocado, and strawberry from Israel and isolates of the pink subpopulation from almond from the United States were characterized by various molecular methods and compared with morphological identification. Taxon-specific primer analysis grouped the avocado isolates...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2000.90.6.608

    authors: Freeman S,Minz D,Jurkevitch E,Maymon M,Shabi E

    更新日期:2000-06-01 00:00:00

  • Reproductive Ability of Hybrids of Striga aspera and Striga hermonthica.

    abstract::ABSTRACT Striga aspera and S. hermonthica are sympatric in Africa. Each may serve as virulent gene reservoirs for the other if they hybridize and their hybrids are virulent and fertile. Intraspecific and interspecific crosses were made within and between the species, and reproductive success was determined. Freshly ha...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1998.88.6.563

    authors: Aigbokhan EI,Berner DK,Musselman LJ

    更新日期:1998-06-01 00:00:00

  • Extending the fungal host range of a partitivirus and a mycoreovirus from Rosellinia necatrix by inoculation of protoplasts with virus particles.

    abstract::The potential host range of mycoviruses is poorly understood because of the lack of suitable inoculation methods. Recently, successful transfection has been reported for somatically incompatible fungal isolates with purified virus particles of two mycoviruses, the partitivirus RnPV1-W8 (RnPV1) and the mycoreovirus RnM...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-100-9-0922

    authors: Kanematsu S,Sasaki A,Onoue M,Oikawa Y,Ito T

    更新日期:2010-09-01 00:00:00

  • Hypovirulence and Double-Stranded RNA in Botrytis cinerea.

    abstract::ABSTRACT Twenty-one strains of Botrytis cinerea isolated from 13 species of plants grown in China were compared for pathogenicity on Brassica napus, mycelial growth on potato dextrose agar, and presence of double-stranded (ds)RNA. The results showed that the strain CanBc-1 was severely debilitated in pathogenicity and...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-12-1590

    authors: Wu MD,Zhang L,Li GQ,Jiang DH,Hou MS,Huang HC

    更新日期:2007-12-01 00:00:00

  • Detection of citrus huanglongbing-associated 'Candidatus Liberibacter asiaticus' in citrus and Diaphorina citri in Pakistan, seasonal variability, and implications for disease management.

    abstract::We report the detection of the huanglongbing (HLB)-associated bacterium 'Candidatus Liberibacter asiaticus' from both plants and insects in Pakistan and the seasonal variability in the numbers of 'Ca. L. asiaticus'-positive psyllid vector, Diaphorina citri. Our studies showed that 'Ca. L. asiaticus' was detectable fro...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-08-13-0224-R

    authors: Razi MF,Keremane ML,Ramadugu C,Roose M,Khan IA,Lee RF

    更新日期:2014-03-01 00:00:00

  • Sequence and simple-sequence repeat analyses of the fungal pathogen Seiridium cardinale indicate California is the most likely source of the cypress canker epidemic for the Mediterranean region.

    abstract::Seiridium cardinale is the pathogenic fungus of unknown origin responsible for a world pandemic known as cypress canker affecting several species of Cupressaceae in both the Northern and Southern Hemisphere. In this study, a comparative genetic analysis of worldwide populations was performed using sequence analysis of...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-05-11-0144

    authors: Della Rocca G,Eyre CA,Danti R,Garbelotto M

    更新日期:2011-12-01 00:00:00

  • Population Structure of Phytophthora cinnamomi in South Africa.

    abstract::ABSTRACT Phytophthora cinnamomi isolates collected from 1977 to 1986 and 1991 to 1993 in two regions in South Africa were analyzed using isozymes. A total of 135 isolates was analyzed for 14 enzymes representing 20 putative loci, of which four were polymorphic. This led to the identification of nine different multiloc...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1997.87.8.822

    authors: Linde C,Drenth A,Kemp GH,Wingfield MJ,von Broembsen SL

    更新日期:1997-08-01 00:00:00

  • Pathogenicity and In Planta Mycotoxin Accumulation Among Members of the Fusarium graminearum Species Complex on Wheat and Rice.

    abstract::ABSTRACT Fusarium head blight (FHB), or scab, is a destructive disease of small grains caused by members of the Fusarium graminearum species complex, comprised of at least nine distinct, cryptic species. Members of this complex are known to produce mycotoxins including the trichothecenes deoxynivalenol (DON) along wit...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-1397

    authors: Goswami RS,Kistler HC

    更新日期:2005-12-01 00:00:00

  • Development of a variable number of tandem repeats typing scheme for the bacterial rice pathogen Xanthomonas oryzae pv. oryzicola.

    abstract::Xanthomonas oryzae pv. oryzicola is an important bacterial pathogen responsible for outbreaks of bacterial leaf streak (BLS) on rice, mostly occurring in Asia and parts of Africa. To better monitor epidemics and assess population structures, efficient tools that allow the precise identification and diagnosis of pathog...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-04-12-0078-R

    authors: Zhao S,Poulin L,Rodriguez-R LM,Serna NF,Liu SY,Wonni I,Szurek B,Verdier V,Leach JE,He YQ,Feng JX,Koebnik R

    更新日期:2012-10-01 00:00:00

  • Patterns of Virulence in Natural Populations of Puccinia coronata on Wild Oat in Israel and in Agricultural Populations on Cultivated Oat in the United States.

    abstract::ABSTRACT Crown rust (Puccinia coronata) in indigenous populations of Avena sterilis has been cited as an example of stability of wild pathosystems that consist of natural mixtures of resistance and virulence. This study confirmed that virulence/avirulence polymorphisms in P. coronata on A. sterilis in Israel are highl...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.5.505

    authors: Leonard KJ,Anikster Y,Manisterski J

    更新日期:2004-05-01 00:00:00

  • Resistance to the Emerging Moroccan Watermelon Mosaic Virus in Squash.

    abstract::Moroccan watermelon mosaic virus (MWMV) represents an emerging threat to cucurbit production in the Mediterranean Basin. We sequenced the near complete genome of MWMV-SQ10_1.1, a cloned Spanish isolate. MWMV-SQ10_1.1 has the typical potyvirus genomic structure, and phylogenetic analysis showed that it shared a common ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-10-18-0395-R

    authors: Miras M,Juárez M,Aranda MA

    更新日期:2019-05-01 00:00:00

  • Persistence and spatial autocorrelation of clones of Erysiphe necator overwintering as mycelium in dormant buds in an isolated vineyard in northern Italy.

    abstract::The population structure of the grape powdery mildew fungus, Erysiphe necator (formerly Uncinula necator), has been hypothesized to vary from being clonal to highly diverse and recombining. We report here on the structure of an E. necator population sampled during a 4-year period from an isolated vineyard in northern ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-98-2-0148

    authors: Cortesi P,Pizzatti C,Bertocchi D,Milgroom MG

    更新日期:2008-02-01 00:00:00

  • Association of Little cherry virus 1 (LChV1) with the Shirofugen stunt disease and characterization of the genome of a divergent LChV1 isolate.

    abstract::Double-stranded RNAs purified from the V2356 ('Successa') sour cherry source of the Shirofugen stunt disease (SSD) were sequenced using a 454 pyrosequencing multiplex approach. The 15,646 reads obtained were assembled into 279 contigs, 5 of which, totaling almost 16.9 kbp and 5,332 reads (34% of sample reads), showed ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-10-12-0275-R

    authors: Candresse T,Marais A,Faure C,Gentit P

    更新日期:2013-03-01 00:00:00

  • Genetic and Phenotypic Analysis of Soybean mosaic virus Resistance in PI 88788 Soybean.

    abstract::ABSTRACT Resistance to Soybean mosaic virus (SMV) was identified in PI 88788 soybean, a germ plasm accession from China that is used widely as a source of resistance to soybean cyst nematode. Strains SMV-G1 through -G7 infected the inoculated leaves of PI 88788 but were not detected in upper, noninoculated trifoliolat...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.7.687

    authors: Gunduz I,Buss GR,Chen P,Tolin SA

    更新日期:2004-07-01 00:00:00

  • Purification and Biological Characterization of Host-Specific SV-Toxins from Stemphylium vesicarium Causing Brown Spot of European Pear.

    abstract::ABSTRACT Culture filtrates of a pathogenic isolate (IT37) of Stemphylium vesicarium, causing brown spot of European pear, induced veinal necrosis only on pear leaves susceptible to the pathogen. Two host-specific toxins, SV-toxins I and II, were purified from culture filtrates of IT37 by successively using Amberlite X...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.10.947

    authors: Singh P,Bugiani R,Cavanni P,Nakajima H,Kodama M,Otani H,Kohmoto K

    更新日期:1999-10-01 00:00:00

  • Localized Melanization of Appressoria Is Required for Pathogenicity of Venturia inaequalis.

    abstract::ABSTRACT During formation of appressoria produced from conidia and ascospores of Venturia inaequalis, a dark brown ring structure was detected at the base of appressoria. This melanized appressorial ring structure (MARS) was attached to the leaf surface like a sealing ring and formed the fungus-plant interface; it is ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-10-1222

    authors: Steiner U,Oerke EC

    更新日期:2007-10-01 00:00:00

  • Characterization of a Viral EPS-Depolymerase, a Potential Tool for Control of Fire Blight.

    abstract::ABSTRACT A 3.3-kb fragment of genomic DNA from bacteriophage Phi-Ea1h encoding an amylovoran-directed depolymerase lyase was sequenced, and three open reading frames (ORFs) were detected. The first ORF could encode a lysozyme and the second a holin that may form a pore supporting cell lysis by the lysozyme. The third ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2000.90.11.1263

    authors: Kim WS,Geider K

    更新日期:2000-11-01 00:00:00

  • Characterization of Antagonistic Bacillus methylotrophicus Isolated From Rhizosphere and Its Biocontrol Effects on Maize Stalk Rot.

    abstract::Stalk rot is one of the most serious and widespread diseases in maize, and effective control measures are currently lacking. Therefore, this study aimed to develop a new biological agent to manage this disease. An antagonistic bacterial strain, TA-1, was isolated from rhizosphere soil and identified as Bacillus methyl...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-07-18-0220-R

    authors: Cheng X,Ji X,Ge Y,Li J,Qi W,Qiao K

    更新日期:2019-04-01 00:00:00

  • A Single Locus Leads to Resistance of Arabidopsis thaliana to Bacterial Wilt Caused by Ralstonia solanacearum Through a Hypersensitive-like Response.

    abstract::ABSTRACT Strains of Ralstonia solanacearum have been shown to cause bacterial wilt in some, but not all, ecotypes of Arabidopsis thaliana. We demonstrate here that after inoculation of the leaves of resistant ecotype S96 with R. solanacearum strain Ps95 necrosis around the inoculation site rapidly appeared and no furt...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.8.673

    authors: Ho GD,Yang CH

    更新日期:1999-08-01 00:00:00

  • LtEPG1, a Secretory Endopolygalacturonase Protein, Regulates the Virulence of Lasiodiplodia theobromae in Vitis vinifera and Is Recognized as a Microbe-Associated Molecular Patterns.

    abstract::The Lasiodiplodia theobromae genome encodes numerous glycoside hydrolases involved in organic matter degradation and conducive to pathogen infection, whereas their molecular mechanisms are still largely unknown. Here, we identified the glycoside hydrolase family 28 endopolygalacturonase LtEPG1 in L. theobromae and cha...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-04-20-0118-R

    authors: Thilini Chethana KW,Peng J,Li X,Xing Q,Liu M,Zhang W,Hyde KD,Zhao W,Yan J

    更新日期:2020-10-01 00:00:00

  • Characterization of the Dual Subcellular Localization of Lilium LsGRP1, a Plant Class II Glycine-Rich Protein.

    abstract::The defense-related gene LsGRP1 exhibits an increased level of expression in Lilium spp. after being infected by Botrytis elliptica, the fungal pathogen of lily leaf blight. In this study, the expression profile of the LsGRP1 protein (a plant class II glycine-rich protein) was characterized biochemically and its subce...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-01-14-0020-R

    authors: Lin CH,Chen CY

    更新日期:2014-10-01 00:00:00

  • Wheat (Triticum aestivum) Is Susceptible to the Parasitic Angiosperm Striga hermonthica, a Major Cereal Pathogen in Africa.

    abstract::ABSTRACT Striga hermonthica is a parasitic weed endemic to sub-Saharan Africa. It most commonly parasitizes sorghum, maize, pearl millet, and upland rice, lowering yields and affecting the welfare of over 100 million people, principally subsistence farmers. Cereal crops with complete resistance to this pathogen have n...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-1294

    authors: Vasey RA,Scholes JD,Press MC

    更新日期:2005-11-01 00:00:00