Purification and Biological Characterization of Host-Specific SV-Toxins from Stemphylium vesicarium Causing Brown Spot of European Pear.

Abstract:

:ABSTRACT Culture filtrates of a pathogenic isolate (IT37) of Stemphylium vesicarium, causing brown spot of European pear, induced veinal necrosis only on pear leaves susceptible to the pathogen. Two host-specific toxins, SV-toxins I and II, were purified from culture filtrates of IT37 by successively using Amberlite XAD-2 resin adsorption, cellulose thin-layer chromatography, and high-performance liquid chromatography under three different sets of conditions. Susceptible cultivars showed veinal necrosis at a SV-toxin I concentration of 0.01 to 0.1 mug/ml, whereas resistant cultivars were insensitive to the toxin at 1,000 mug/ml. SV-toxins I and II caused a dose-dependent increase in electrolyte loss from susceptible leaf tissues. No increase in electrolyte loss was detected in leaf tissues from resistant cultivars. The results of physiological studies indicated that SV-toxins appear to have an early effect on plasma membranes of susceptible leaves. Spores of a nonpathogenic isolate induced necrotic lesions on susceptible leaves in the presence of a small amount of toxin. SV-toxins were detected in intercellular fluids obtained from diseased leaves after inoculation with the pathogen. The results indicate that SV-toxins I and II produced by S. vesicarium can be characterized as host-specific toxins.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Singh P,Bugiani R,Cavanni P,Nakajima H,Kodama M,Otani H,Kohmoto K

doi

10.1094/PHYTO.1999.89.10.947

subject

Has Abstract

pub_date

1999-10-01 00:00:00

pages

947-53

issue

10

eissn

0031-949X

issn

1943-7684

journal_volume

89

pub_type

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