Weakening and Delayed Mortality of Fusarium oxysporum by Heat Treatment: Flow Cytometry and Growth Studies.

Abstract:

:ABSTRACT Survival of Fusarium oxysporum f. sp. niveum following heat treatments was studied using flow cytometric, physiological, and microscopic assays. We exposed germinating conidia to sublethal temperatures from 36 to 42 degrees C for 60 min, followed by rhodamine 123 staining and flow cytometry, and found increasing levels of fluorescence that reflect a change in mitochondrial membrane potential, indicating a weakening induced by stress. Viability of conidia or germinating conidia of the fungus exposed to heat decreased with increasing temperature, as assessed by fluorescent staining. However, viability was higher than that assessed with the 5-day-long plate count method and was further reduced 13 and 24 h after treatment, suggesting delayed mortality of the heat-treated germinating conidia. Delayed mortality was substantiated by observing these conidia with light and fluorescent scanning electron microscopy and by subculturing single germinating conidia that had been previously heated. Programmed cell death was not observed in heat-treated conidia or germinating conidia of F. oxysporum based on the detection of plasma membrane phosphatidylserine translocation, cell-cycle measurements, detection of DNA fragmentation, or microscopic observation of apoptotic bodies. We hypothesize that propagules, which survived the heating and apparently are alive, may undergo further irreversible detrimental processes, eventually leading to their death by yet unidentified mechanisms. These findings suggest that pathogen propagules also might be affected under lower temperatures, possibly facilitating pathogen control by heating.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Assaraf MP,Ginzburg C,Katan J

doi

10.1094/PHYTO.2002.92.9.956

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

956-63

issue

9

eissn

0031-949X

issn

1943-7684

journal_volume

92

pub_type

杂志文章
  • A Plant Nutrient- and Microbial Protein-Based Resistance Inducer Elicits Wheat Cultivar-Dependent Resistance Against Zymoseptoria tritici.

    abstract::The induction of plant defense mechanisms by resistance inducers is an attractive and innovative alternative to reduce the use of fungicides on wheat against Zymoseptoria tritici, the responsible agent of Septoria tritici blotch (STB). Under controlled conditions, we investigated the resistance induction in three whea...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-03-19-0075-R

    authors: Ors M,Randoux B,Siah A,Couleaud G,Maumené C,Sahmer K,Reignault P,Halama P,Selim S

    更新日期:2019-12-01 00:00:00

  • Meta-analysis of the effects of triazole-based fungicides on wheat yield and test weight as influenced by Fusarium head blight intensity.

    abstract::ABSTRACT Multivariate random-effects meta-analyses were conducted on 12 years of data from 14 U.S. states to determine the mean yield and test-weight responses of wheat to treatment with propiconazole, prothioconazole, tebuconazole, metconazole, and prothioconazole+tebuconazole. All fungicides led to a significant inc...

    journal_title:Phytopathology

    pub_type: 杂志文章,meta分析

    doi:10.1094/PHYTO-100-2-0160

    authors: Paul PA,McMullen MP,Hershman DE,Madden LV

    更新日期:2010-02-01 00:00:00

  • Metallothionein and bZIP Transcription Factor Genes from Velvetleaf and Their Differential Expression Following Colletotrichum coccodes Infection.

    abstract::ABSTRACT Colletotrichum coccodes is a biocontrol agent of velvetleaf (Abutilon theophrasti), a noxious weed of corn and soybean. Metallothioneins (MTs) and basic region/leucine zipper motif (bZIP) are heavy-metal-binding proteins and transcription factors, respectively, that have been related to several plant processe...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-96-1116

    authors: Dauch AL,Jabaji-Hare SH

    更新日期:2006-10-01 00:00:00

  • Isolation, Selection, and Efficacy of Pochonia chlamydosporia for Control of Rotylenchulus reniformis on Cotton.

    abstract::ABSTRACT The reniform nematode, Rotylenchulus reniformis, is a serious threat to cotton (Gossypium hirsutum) production in the United States, causing an annual loss of about $80 million. The objective of this study was to isolate fungi from eggs of R. reniformis and select potential biocontrol agents for R. reniformis...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-0890

    authors: Wang K,Riggs RD,Crippen D

    更新日期:2005-08-01 00:00:00

  • Etiology and Epidemiological Conditions Promoting Fusarium Root Rot in Sweetpotato.

    abstract::Sweetpotato production in the United States is limited by several postharvest diseases, and one of the most common is Fusarium root rot. Although Fusarium solani is believed to be the primary causal agent of disease, numerous other Fusarium spp. have been reported to infect sweetpotato. However, the diversity of Fusar...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-01-16-0009-R

    authors: Scruggs AC,Quesada-Ocampo LM

    更新日期:2016-08-01 00:00:00

  • A High-Throughput Microtiter-Based Fungicide Sensitivity Assay for Oomycetes Using Z'-Factor Statistic.

    abstract::Current methods to quantitatively assess fungicide sensitivity for a diverse range of oomycetes are slow and labor intensive. Microtiter-based assays can be used to increase throughput. However, many factors can affect their quality and reproducibility. Therefore, efficient and reliable methods for detection of assay ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-01-19-0018-R

    authors: Noel ZA,Rojas AJ,Jacobs JL,Chilvers MI

    更新日期:2019-09-01 00:00:00

  • Characterization of a synergistic interaction between two cucurbit-infecting begomoviruses: Squash leaf curl virus and Watermelon chlorotic stunt virus.

    abstract::Squash leaf curl virus (SLCV) and Watermelon chlorotic stunt virus (WmCSV) are cucurbit-infecting bipartite begomoviruses. Both viruses are found in the eastern Mediterranean basin but the effects of dual infection of both viruses on melon (Cucumis melo L.) have not been described. 'Arava' melon plants were inoculated...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-06-10-0159

    authors: Sufrin-Ringwald T,Lapidot M

    更新日期:2011-02-01 00:00:00

  • Molecular mapping of the major resistance quantitative trait locus qHS2.09 with simple sequence repeat and single nucleotide polymorphism markers in maize.

    abstract::The major quantitative trait locus (QTL) qHS2.09 plays an important role in resistance to head smut during maize breeding and production. In this study, a near-isogenic line (NIL), L34, which harbors the major QTL qHS2.09 in bin 2.09, was developed using a resistant donor 'Mo17' in a susceptible genetic background 'Hu...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-12-11-0330

    authors: Weng J,Liu X,Wang Z,Wang J,Zhang L,Hao Z,Xie C,Li M,Zhang D,Bai L,Liu C,Zhang S,Li X

    更新日期:2012-07-01 00:00:00

  • Cpvma1, a Vacuolar H+-ATPase Catalytic Subunit of Cryphonectria parasitica, is Essential for Virulence and Hypovirus RNA Accumulation.

    abstract::The vacuolar H+-ATPases (V-ATPases) are conserved ATP-dependent proton pumps that acidify intracellular compartments in eukaryotic cells. The role of Cpvma1, a V-ATPase catalytic subunit A of Cryphonectria parasitica, was investigated by generating cpvma1-overexpressing and cpvma1-silenced strains. The mutant strains ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-08-18-0289-R

    authors: Li R,Bai S,He Y,Chen Q,Yao Y,Wang J,Chen B

    更新日期:2019-08-01 00:00:00

  • Transcriptional Reprogramming of the Mycoparasitic Fungus Ampelomyces quisqualis During the Powdery Mildew Host-Induced Germination.

    abstract::Ampelomyces quisqualis is a mycoparasite of a diverse range of phytopathogenic fungi associated with the powdery mildew disease. Among them are several Erysiphaceae species with great economic impact on high-value crops such as grape. Due to its ability to parasitize and prevent the spread of powdery mildews, A. quisq...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-01-14-0013-R

    authors: Siozios S,Tosi L,Ferrarini A,Ferrari A,Tononi P,Bellin D,Maurhofer M,Gessler C,Delledonne M,Pertot I

    更新日期:2015-02-01 00:00:00

  • Biotic, molecular, and phylogenetic characterization of bean calico mosaic virus, a distinct begomovirus species with affiliation in the squash leaf curl virus cluster.

    abstract::ABSTRACT Bean calico mosaic virus (BCMoV), a whitefly-transmitted geminivirus from Sonora, Mexico, was purified, and the genome components were cloned and sequenced. Purified viral fractions and cloned genome components were infectious by biolistic inoculation to bean, completing Koch's postulates for both. The B biot...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.4.273

    authors: Brown JK,Ostrow KM,Idris AM,Stenger DC

    更新日期:1999-04-01 00:00:00

  • F240 of β2-Tubulin Explains why Fusarium graminearum is Less Sensitive to Carbendazim than Botrytis cinerea.

    abstract::β-Tubulin is the target of benzimidazole fungicides, the most widely used of which is carbendazim (methyl benzimidazol-2-ylcarbamate [MBC]). MBC sensitivity is determined by the differential affinity of MBC for β-tubulins. However, the mechanism of less sensitivity of Fusarium graminearum to MBC compared with other fu...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-09-17-0295-R

    authors: Zhu Y,Liang X,Li Y,Duan Y,Zheng Z,Wang J,Zhou M

    更新日期:2018-03-01 00:00:00

  • Inhibitory effects of antioxidant reagent in reactive oxygen species generation and penetration of appressoria of Alternaria alternata Japanese pear pathotype.

    abstract::In the Japanese pear pathotype of Alternaria alternata, H2O2 is generated solely from penetration pegs and not from other portions of subcuticular hyphae within the pectin layers of host leaves. A close association between H2O2 generation and fungal aggressiveness is expected because the pegs are important for fungal ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-100-9-0840

    authors: Hyon GS,Ikeda K,Hosogi N,Shinogi T,Park P

    更新日期:2010-09-01 00:00:00

  • Identification of Transcripts Involved in Resistance Responses to Leaf Spot Disease Caused by Cercosporidium personatum in Peanut (Arachis hypogaea).

    abstract::ABSTRACT Late leaf spot disease caused by Cercosporidium personatum is one of the most destructive foliar diseases of peanut (Arachis hypogaea) worldwide. The objective of this research was to identify resistance genes in response to leaf spot disease using microarray and real-time polymerase chain reaction (PCR). To ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-95-0381

    authors: Luo M,Dang P,Bausher MG,Holbrook CC,Lee RD,Lynch RE,Guo BZ

    更新日期:2005-04-01 00:00:00

  • Association of Effector Six6 with Vascular Wilt Symptoms Caused by Fusarium oxysporum on Soybean.

    abstract::The Fusarium oxysporum species complex (FOSC) is a widely distributed group of fungi that includes both pathogenic and nonpathogenic isolates. In a previous study, isolates within the FOSC collected primarily from soybean were assessed for the presence of 12 fungal effector genes. Although none of the assayed genes wa...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-03-16-0118-R

    authors: Lanubile A,Ellis ML,Marocco A,Munkvold GP

    更新日期:2016-11-01 00:00:00

  • Characterization of Bois noir isolates by restriction fragment length polymorphism of a Stolbur-specific putative membrane protein gene.

    abstract::Bois noir phytoplasma (BNp), widespread in wine-producing areas of Europe and endemic in France and Italy, is classified in the 16SrXII-A subgroup, whose members are referred to as Stolbur phytoplasmas. The 16S rDNA gene of Stolbur phytoplasma shows low variability, and few non-ribosomal genes are available as markers...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-99-6-0711

    authors: Pacifico D,Alma A,Bagnoli B,Foissac X,Pasquini G,Tessitori M,Marzachì C

    更新日期:2009-06-01 00:00:00

  • A methodology to detect and quantify five pathogens causing potato tuber decay using real-time quantitative polymerase chain reaction.

    abstract::ABSTRACT Late blight (Phytophthora infestans), pink rot (Phytophthora erythroseptica), leak (Pythium ultimum), dry rot (Fusarium sambucinum), and soft rot (Erwinia carotovora subsp. carotovora and subsp. atroseptica) are particularly damaging diseases of stored potato tubers worldwide. In this study, we present a meth...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-96-1037

    authors: Atallah ZK,Stevenson WR

    更新日期:2006-09-01 00:00:00

  • Networks in plant epidemiology: from genes to landscapes, countries, and continents.

    abstract::There is increasing use of networks in ecology and epidemiology, but still relatively little application in phytopathology. Networks are sets of elements (nodes) connected in various ways by links (edges). Network analysis aims to understand system dynamics and outcomes in relation to network characteristics. Many exi...

    journal_title:Phytopathology

    pub_type: 杂志文章,评审

    doi:10.1094/PHYTO-07-10-0192

    authors: Moslonka-Lefebvre M,Finley A,Dorigatti I,Dehnen-Schmutz K,Harwood T,Jeger MJ,Xu X,Holdenrieder O,Pautasso M

    更新日期:2011-04-01 00:00:00

  • Sequence and simple-sequence repeat analyses of the fungal pathogen Seiridium cardinale indicate California is the most likely source of the cypress canker epidemic for the Mediterranean region.

    abstract::Seiridium cardinale is the pathogenic fungus of unknown origin responsible for a world pandemic known as cypress canker affecting several species of Cupressaceae in both the Northern and Southern Hemisphere. In this study, a comparative genetic analysis of worldwide populations was performed using sequence analysis of...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-05-11-0144

    authors: Della Rocca G,Eyre CA,Danti R,Garbelotto M

    更新日期:2011-12-01 00:00:00

  • Segregation of distinct variants from Citrus tristeza virus isolate SY568 using aphid transmission.

    abstract::A well-studied severe isolate of Citrus tristeza virus (CTV) known as SY568 has previously been shown to contain multiple variants of the virus which differ in their genetic and biological characters. Aphid transmission was used in an attempt to segregate some of these variants for further characterization. Resulting ...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-99-10-1168

    authors: Velazquez-Monreal JJ,Mathews DM,Dodds JA

    更新日期:2009-10-01 00:00:00

  • Cacao diseases: a global perspective from an industry point of view.

    abstract::ABSTRACT Diseases of cacao, Theobroma cacao, account for losses of more than 30% of the potential crop. These losses have caused a steady decline in production and a reduction in bean quality in almost all the cacao-producing areas in the world, especially in small-holder farms in Latin America and West Africa. The mo...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-12-1658

    authors: Hebbar PK

    更新日期:2007-12-01 00:00:00

  • Diversity of Xanthomonas campestris Isolates from Symptomatic Crucifers in New York State.

    abstract::To assess the diversity of Xanthomonas campestris spp. infecting crucifers in New York, 154 isolates were collected over 10 years across the state. The goal was to determine if isolates of the pathogen were overwintering in New York and serving as primary inoculum in subsequent years, or if novel isolates were enterin...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-06-15-0134-R

    authors: Lange HW,Tancos MA,Carlson MO,Smart CD

    更新日期:2016-02-01 00:00:00

  • An integrated badnavirus is prevalent in fig germplasm.

    abstract::Fig mosaic occurs worldwide and is the most common and important viral disease of fig. In the quest to identify the causal agent of the disease, several new viruses have been identified, including a new DNA virus, the subject of this communication. Phylogenetic analysis placed the virus, provisionally named Fig badnav...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-12-11-0351

    authors: Laney AG,Hassan M,Tzanetakis IE

    更新日期:2012-12-01 00:00:00

  • Role of phenology in host susceptibility and within-plant spread of stem rust during reproductive development of perennial ryegrass.

    abstract::ABSTRACT Perennial ryegrass (Lolium perenne) was inoculated with urediniospores of Puccinia graminis subsp. graminicola at four stages of reproductive tiller development. All developmental stages, from expansion of the penultimate leaf through anthesis, were equally susceptible to infection measured as number of pustu...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2004.94.3.308

    authors: Pfender WF

    更新日期:2004-03-01 00:00:00

  • Anisotropy, in density and in distance, of the dispersal of yellow rust of wheat: experiments in large field plots and estimation.

    abstract::ABSTRACT Long-distance dispersal of spores generally presents anisotropy. This anisotropy can appear in the mean number of spores deposited along a given direction (anisotropy in density) and in the mean distance that a spore travels in a given direction (anisotropy in distance). Specific experiments together with a s...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-97-10-1315

    authors: Soubeyrand S,Enjalbert J,Sanchez A,Sache I

    更新日期:2007-10-01 00:00:00

  • Regional Assessment of Soybean Brown Stem Rot, Phytophthora sojae, and Heterodera glycines Using Area-Frame Sampling: Prevalence and Effects of Tillage.

    abstract::ABSTRACT The prevalence of brown stem rot (caused by Phialophora gregata), Heterodera glycines, and Phytophthora sojae in the north central United States was investigated during the fall of 1995 and 1996. Soybean fields were randomly selected using an area-frame sampling design in collaboration with the National Agric...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1999.89.3.204

    authors: Workneh F,Tylka GL,Yang XB,Faghihi J,Ferris JM

    更新日期:1999-03-01 00:00:00

  • Characterization of a variant of Xanthomonas citri subsp. citri that triggers a host-specific defense response.

    abstract::Citrus is an economically important fruit crop that is severely afflicted by Asiatic citrus bacterial canker (CBC), a disease caused by the phytopathogen Xanthomonas citri subsp. citri (X. citri). To gain insight into the molecular epidemiology of CBC, 42 Xanthomonas isolates were collected from a range of Citrus spp....

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-11-12-0287-R

    authors: Chiesa MA,Siciliano MF,Ornella L,Roeschlin RA,Favaro MA,Delgado NP,Sendín LN,Orce IG,Ploper LD,Vojnov AA,Vacas JG,Filippone MP,Castagnaro AP,Marano MR

    更新日期:2013-06-01 00:00:00

  • Draft Whole Genome Sequence Analyses on Pseudomonas syringae pv. actinidiae Hypersensitive Response Negative Strains Detected from Kiwifruit Bleeding Sap Samples.

    abstract::Kiwifruit bleeding sap samples, collected in Italian and Chilean orchards from symptomatic and asymptomatic plants, were evaluated for the presence of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker. The saps were sampled during the spring in both hemispheres, before the bud sprouting, during...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO-08-17-0278-R

    authors: Biondi E,Zamorano A,Vega E,Ardizzi S,Sitta D,De Salvador FR,Campos-Vargas R,Meneses C,Perez S,Bertaccini A,Fiore N

    更新日期:2018-05-01 00:00:00

  • Corn Seed Proteins Inhibitory to Aspergillus flavus and Aflatoxin Biosynthesis.

    abstract::ABSTRACT This study reports the presence of two fractions from corn seeds inhibitory to aflatoxin formation. Using a sensitive laboratory assay that can measure both inhibition of fungal growth and inhibition of aflatoxin biosynthesis, we examined aqueous extracts from seeds of Tex6, a corn inbred shown to be highly r...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.1997.87.6.622

    authors: Huang Z,White DG,Payne GA

    更新日期:1997-06-01 00:00:00

  • An Evaluation of the Genetic Diversity of Xylella fastidiosa Isolated from Diseased Citrus and Coffee in São Paulo, Brazil.

    abstract::ABSTRACT Strains of Xylella fastidiosa, isolated from sweet orange trees (Citrus sinensis) and coffee trees (Coffea arabica) with symptoms of citrus variegated chlorosis and Requeima do Café, respectively, were indistinguishable based on repetitive extragenic palindromic polymerase chain reaction (PCR) and enterobacte...

    journal_title:Phytopathology

    pub_type: 杂志文章

    doi:10.1094/PHYTO.2001.91.6.599

    authors: Qin X,Miranda VS,Machado MA,Lemos EG,Hartung JS

    更新日期:2001-06-01 00:00:00