Characterization of a variant of Xanthomonas citri subsp. citri that triggers a host-specific defense response.

Abstract:

:Citrus is an economically important fruit crop that is severely afflicted by Asiatic citrus bacterial canker (CBC), a disease caused by the phytopathogen Xanthomonas citri subsp. citri (X. citri). To gain insight into the molecular epidemiology of CBC, 42 Xanthomonas isolates were collected from a range of Citrus spp. across 17 different orchards in Tucumán, Argentina and subjected to molecular, biochemical, and pathogenicity tests. Analysis of genome-specific X. citri markers and DNA polymorphisms based on repetitive elements-based polymerase chain reaction showed that all 42 isolates belonged to X. citri. Interestingly, pathogenicity tests showed that one isolate, which shares >90% genetic similarity to the reference strain X. citri T, has host range specificity. This new variant of X. citri subsp. citri, named X. citri A(T), which is deficient in xanthan production, induces an atypical, noncankerous chlorotic phenotype in Citrus limon and C. paradisi and weak cankerous lesions in C. aurantifolia and C. clementina leaves. In C. limon, suppression of canker development is concomitant with an oxidative burst; xanthan is not implicated in the phenotype induced by this interaction, suggesting that other bacterial factors would be involved in triggering the defense response.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Chiesa MA,Siciliano MF,Ornella L,Roeschlin RA,Favaro MA,Delgado NP,Sendín LN,Orce IG,Ploper LD,Vojnov AA,Vacas JG,Filippone MP,Castagnaro AP,Marano MR

doi

10.1094/PHYTO-11-12-0287-R

subject

Has Abstract

pub_date

2013-06-01 00:00:00

pages

555-64

issue

6

eissn

0031-949X

issn

1943-7684

journal_volume

103

pub_type

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