Vegetative Compatibility Groups in Colletotrichum coccodes, the Causal Agent of Black Dot on Potato.

Abstract:

:ABSTRACT Black dot of potato, caused by Colletotrichum coccodes, is a disease of growing economic importance, but the degree of genetic diversity and pathogenic differentiation among isolates is unknown. Using nitrate auxotrophic (Nit) mutants, we characterized vegetative compatibility groups (VCG) diversity for C. coccodes for 110 isolates originating from Israel, The Netherlands, and France. We recovered frequencies of nit1 and NitM mutant classes at 38.5 and 7.2%, respectively, and selected 12 isolates as tester isolates. Using these testers, we defined four multimember VCGs at 7.3, 35.5, 20.0, and 10.0% frequency in this sample. Thirty isolates (27.3% of all tested isolates) could not be assigned to any of the major groups, and showed only self-compatibility. The frequency of recovery of Nit mutant sectors was highest in isolates from VCG4, with 50.9 and 13.6% recovery for nit1 and NitM, respectively. However, we did not detect differences in the frequency of mutant classes among the three countries of origin. In pathogenicity tests, isolates from VCG3 were the most aggressive to potato, as expressed by high stem colonization levels and sclerotia density on root and crown. These results suggest that there is significant VCG diversity in this species and that this VCG diversity may be correlated with pathogenic characteristics or specialization.

journal_name

Phytopathology

journal_title

Phytopathology

authors

Nitzan N,Hazanovsky M,Tal M,Tsror Lahkim L

doi

10.1094/PHYTO.2002.92.8.827

subject

Has Abstract

pub_date

2002-08-01 00:00:00

pages

827-32

issue

8

eissn

0031-949X

issn

1943-7684

journal_volume

92

pub_type

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