Census of Candidatus Liberibacter asiaticus population inside the phloem of citrus trees.

Abstract:

:Candidatus Liberibacter asiaticus (CLas) is the predominant causal agent of citrus huanglongbing (HLB). The pathogen population size in local tissues and the whole plant are critical for the development of disease symptoms via pathogenicity factors and causing metabolic burden to the host. However, the total population size of CLas in a whole plant and the ratio of CLas vs. citrus cells in local tissues have not been addressed previously. The total CLas population size for 2.5-year-old Valencia sweet orange trees was quantified using quantitative PCR to be approximately 1.74 x 109, whereas that of 7 and 20-year-old sweet orange trees were estimated to be 4.3 x 1010, and 6.0 x 1010, respectively. The majority of CLas cells were distributed in the leaf tissues (55.58%), followed by that in the branch tissues (36.78%), feeder roots (4.75%), trunk (2.39%), and structural root (0.51%) tissues. The ratios of citrus cells vs. CLas cells for branch, leaf, trunk, feeder root, and structural root samples were approximately 39, 44, 153, 191, and 561, respectively, representing the metabolic burden of CLas in different organs. Approximately 0.01% of the total citrus phloem volume was estimated to be occupied by CLas. The CLas titer inside the leaf was estimated to be approximately 1.64 x 106 cells/leaf or 9.2 x 104 cells cm-2 in leaves, approximately 104 times less than that of typical apoplastic bacterial pathogens. This study provides quantitative estimates of phloem colonization by bacterial pathogens and further understands the biology and virulence mechanism of CLas.

journal_name

Phytopathology

journal_title

Phytopathology

authors

N C Vasconcelos F,Li J,Pang Z,Vincent C,Wang N

doi

10.1094/PHYTO-09-20-0388-R

subject

Has Abstract

pub_date

2020-10-22 00:00:00

eissn

0031-949X

issn

1943-7684

pub_type

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