Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran.

Abstract:

INTRODUCTION:The aim of this study was to detect the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene in Escherichia coliisolates. METHODS:Phenotypic screening of 376 E. coli isolates for ESBL was conducted using disk diffusion. ESBL-producing isolates were tested using PCR and specific primers. The bla(CTX-M) cluster was identified using the RFLP method, and its genotype was sequenced. RESULTS:From 202 ESBL-producing E. coli , 185 (91.5%) possessed CTX-M genes. CTX-M-1 subtypes were found in 98% of the isolates. The bla(CTX-M) gene was identical to CTX-M-15. CONCLUSIONS:A high prevalence of CTX-M-1-producing E. coli apparently exists in Shiraz, Iran.

journal_name

Rev Soc Bras Med Trop

authors

Zamani K,Emami A,Bazargani A,Moattari A

doi

10.1590/0037-8682-0315-2014

subject

Has Abstract

pub_date

2015-07-01 00:00:00

pages

479-82

issue

4

eissn

0037-8682

issn

1678-9849

pii

S0037-86822015000400479

journal_volume

48

pub_type

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