Prevalence of extended-spectrum cephalosporin-resistant Escherichia coli in a farrowing farm: ST1121 clone harboring IncHI2 plasmid contributes to the dissemination of bla CMY-2.

Abstract:

:During a regular monitoring of antimicrobial resistance in a farrowing farm in Southern China, 117 Escherichia coli isolates were obtained from sows and piglets. Compared with the isolates from piglets, the isolates from sows exhibited higher resistance rates to the tested cephalosporins. Correspondingly, the total detection rate of the bla CMY-2/bla CTX-M genes in the sow isolates (34.2%) was also significantly higher than that of the piglet isolates (13.6%; p < 0.05). The bla CMY-2 gene had a relatively high prevalence (11.1%) in the E. coli isolates. MLST and PFGE analysis revealed the clonal spread of ST1121 E. coli in most (7/13) of the bla CMY-2-positive isolates. An indistinguishable IncHI2 plasmid harboring bla CMY-2 was also identified in each of the seven ST1121 E. coli isolates. Complete sequence analysis of this IncHI2 plasmid (pEC5207) revealed that pEC5207 may have originated through recombination of an IncHI2 plasmid with a bla CMY-2-carrying IncA/C plasmid like pCFSAN007427_01. In addition to bla CMY-2, pEC5207 also carried other resistance determinants for aminoglycosides (aacA7), sulfonamides (sul1), as well as heavy metals ions, such as Cu and Ag. The susceptibility testing showed that the pEC5207 can mediate both antibiotic and heavy metal resistance. This highlights the role of pEC5207 in co-selection of bla CMY-2-positive isolates under the selective pressure of heavy metals, cephalosporins, and other antimicrobials. In conclusion, clonal spread of an ST1121 type E. coli strain harboring an IncHI2 plasmid contributed to the dissemination of bla CMY-2 in a farrowing farm in Southern China. We also have determined the first complete sequence analysis of a bla CMY-2-carrying IncHI2 plasmid.

journal_name

Front Microbiol

authors

Deng H,Si HB,Zeng SY,Sun J,Fang LX,Yang RS,Liu YH,Liao XP

doi

10.3389/fmicb.2015.01210

subject

Has Abstract

pub_date

2015-11-03 00:00:00

pages

1210

issn

1664-302X

journal_volume

6

pub_type

杂志文章
  • Rapid Purification and Characterization of Mutant Origin Recognition Complexes in Saccharomyces cerevisiae.

    abstract::Purification of the origin recognition complex (ORC) from wild-type budding yeast cells more than two decades ago opened up doors to analyze the initiation of eukaryotic chromosomal DNA replication biochemically. Although revised methods to purify ORC from overproducing cells were reported later, purification of mutan...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00521

    authors: Kawakami H,Ohashi E,Tsurimoto T,Katayama T

    更新日期:2016-04-18 00:00:00

  • Post-translational Modification-Based Regulation of HIV Replication.

    abstract::Human immunodeficiency virus (HIV) relies heavily on the host cellular machinery for production of viral progeny. To exploit cellular proteins for replication and to overcome host factors with antiviral activity, HIV has evolved a set of regulatory and accessory proteins to shape an optimized environment for its repli...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02131

    authors: Chen L,Keppler OT,Schölz C

    更新日期:2018-09-11 00:00:00

  • Human Microbe-Disease Association Prediction With Graph Regularized Non-Negative Matrix Factorization.

    abstract::A microbe is a microscopic organism which may exists in its single-celled form or in a colony of cells. In recent years, accumulating researchers have been engaged in the field of uncovering microbe-disease associations since microbes are found to be closely related to the prevention, diagnosis, and treatment of many ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02560

    authors: He BS,Peng LH,Li Z

    更新日期:2018-11-01 00:00:00

  • Peptidoglycan Muropeptides: Release, Perception, and Functions as Signaling Molecules.

    abstract::Peptidoglycan (PG) is an essential molecule for the survival of bacteria, and thus, its biosynthesis and remodeling have always been in the spotlight when it comes to the development of antibiotics. The peptidoglycan polymer provides a protective function in bacteria, but at the same time is continuously subjected to ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.00500

    authors: Irazoki O,Hernandez SB,Cava F

    更新日期:2019-03-28 00:00:00

  • The Naphthalene Catabolic Genes of Pseudomonas putida BS3701: Additional Regulatory Control.

    abstract::Pseudomonas microorganisms are used for bioremediation of soils contaminated with petroleum hydrocarbons. The overall remediation efficiency is largely dependent on the presence of macro- and micronutrients. Widely varying concentrations of available nitrogen and iron (Fe) in soils were shown to affect residual hydroc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01217

    authors: Pozdnyakova-Filatova I,Petrikov K,Vetrova A,Frolova A,Streletskii R,Zakharova M

    更新日期:2020-06-05 00:00:00

  • Metagenomic Analysis of Bacteria, Fungi, Bacteriophages, and Helminths in the Gut of Giant Pandas.

    abstract::To obtain full details of gut microbiota, including bacteria, fungi, bacteriophages, and helminths, in giant pandas (GPs), we created a comprehensive microbial genome database and used metagenomic sequences to align against the database. We delineated a detailed and different gut microbiota structures of GPs. A total ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01717

    authors: Yang S,Gao X,Meng J,Zhang A,Zhou Y,Long M,Li B,Deng W,Jin L,Zhao S,Wu D,He Y,Li C,Liu S,Huang Y,Zhang H,Zou L

    更新日期:2018-07-31 00:00:00

  • The Atypical Guanylate Kinase MoGuk2 Plays Important Roles in Asexual/Sexual Development, Conidial Septation, and Pathogenicity in the Rice Blast Fungus.

    abstract::Guanylate kinases (GKs), which convert guanosine monophosphate into guanosine diphosphate (GDP), are important for growth and mannose outer chain elongation of cell wall N-linked glycoproteins in yeast. Here, we identified the ortholog of Saccharomyces cerevisiae GK Guk1, named MoGuk1 and a novel family of fungal GKs ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02467

    authors: Cai X,Zhang X,Li X,Liu M,Liu X,Wang X,Zhang H,Zheng X,Zhang Z

    更新日期:2017-12-11 00:00:00

  • Production of the Plant Hormone Auxin by Salmonella and Its Role in the Interactions with Plants and Animals.

    abstract::The ability of human enteric pathogens to colonize plants and use them as alternate hosts is now well established. Salmonella, similarly to phytobacteria, appears to be capable of producing the plant hormone auxin via an indole-3-pyruvate decarboxylase (IpdC), a key enzyme of the IPyA pathway. A deletion of the Salmon...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02668

    authors: Cox CE,Brandl MT,de Moraes MH,Gunasekera S,Teplitski M

    更新日期:2018-01-12 00:00:00

  • Does Mucosal B1 Activation Result in the Accumulation of Peak IgM During Chronic Intrarectal SIVmac239 Exposure to Protect Chinese-Origin Rhesus Macaques From Disease Progression?

    abstract::Human immunodeficiency virus (HIV) infection is characterized by a dynamic process and highly variable progression. Although extensive comparisons have been reported between the minority of non-progressors (NPGs) and the majority of progressors (PGs), the underlying mechanism is still unclear. One reason for this is t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00357

    authors: Cong Z,Tong L,Wang Y,Su A,Chen T,Wei Q,Xue J,Qin C

    更新日期:2020-03-19 00:00:00

  • Heterologous Production of a Novel Cyclic Peptide Compound, KK-1, in Aspergillus oryzae.

    abstract::A novel cyclic peptide compound, KK-1, was originally isolated from the plant-pathogenic fungus Curvularia clavata. It consists of 10 amino acid residues, including five N-methylated amino acid residues, and has potent antifungal activity. Recently, the genome-sequencing analysis of C. clavata was completed, and the b...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00690

    authors: Yoshimi A,Yamaguchi S,Fujioka T,Kawai K,Gomi K,Machida M,Abe K

    更新日期:2018-04-09 00:00:00

  • Disentangling the Drivers of Diversity and Distribution of Fungal Community Composition in Wastewater Treatment Plants Across Spatial Scales.

    abstract::Activated sludge microbial community composition is a key bio-indicator of the sustainability of wastewater treatment systems. Therefore, a thorough understanding of the activated sludge microbial community dynamics is critical for environmental engineers to effectively manage the wastewater treatment plants (WWTPs). ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01291

    authors: Zhang H,Feng J,Chen S,Li B,Sekar R,Zhao Z,Jia J,Wang Y,Kang P

    更新日期:2018-06-18 00:00:00

  • Fold modulating function: bacterial toxins to functional amyloids.

    abstract::Many bacteria produce cytolytic toxins that target host cells or other competing microbes. It is well known that environmental factors control toxin expression, however, recent work suggests that some bacteria manipulate the fold of these protein toxins to control their function. The β-sheet rich amyloid fold is a hig...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00401

    authors: Syed AK,Boles BR

    更新日期:2014-08-01 00:00:00

  • Beneficial Effects of Spices in Food Preservation and Safety.

    abstract::Spices have been used since ancient times. Although they have been employed mainly as flavoring and coloring agents, their role in food safety and preservation have also been studied in vitro and in vivo. Spices have exhibited numerous health benefits in preventing and treating a wide variety of diseases such as cance...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01394

    authors: Gottardi D,Bukvicki D,Prasad S,Tyagi AK

    更新日期:2016-09-21 00:00:00

  • Iron - A Key Nexus in the Virulence of Aspergillus fumigatus.

    abstract::Iron is an essential but, in excess, toxic nutrient. Therefore, fungi evolved fine-tuned mechanisms for uptake and storage of iron, such as the production of siderophores (low-molecular mass iron-specific chelators). In Aspergillus fumigatus, iron starvation causes extensive transcriptional remodeling involving two ce...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00028

    authors: Haas H

    更新日期:2012-02-06 00:00:00

  • Different Transcriptional Responses from Slow and Fast Growth Rate Strains of Listeria monocytogenes Adapted to Low Temperature.

    abstract::Listeria monocytogenes has become one of the principal foodborne pathogens worldwide. The capacity of this bacterium to grow at low temperatures has opened an interesting field of study in terms of the identification and classification of new strains of L. monocytogenes with different growth capacities at low temperat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00229

    authors: Cordero N,Maza F,Navea-Perez H,Aravena A,Marquez-Fontt B,Navarrete P,Figueroa G,González M,Latorre M,Reyes-Jara A

    更新日期:2016-03-01 00:00:00

  • Interaction of antibacterial compounds with RND efflux pumps in Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa infections are becoming increasingly difficult to treat due to intrinsic antibiotic resistance and the propensity of this pathogen to accumulate diverse resistance mechanisms. Hyperexpression of efflux pumps of the Resistance-Nodulation-Cell Division (RND)-type multidrug efflux pumps (e.g., MexAB-Op...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00660

    authors: Dreier J,Ruggerone P

    更新日期:2015-07-08 00:00:00

  • Aspergillus niger Secretes Citrate to Increase Iron Bioavailability.

    abstract::Aspergillus niger has an innate ability to secrete various organic acids, including citrate. The conditions required for A. niger citrate overproduction are well described, but the physiological reasons underlying extracellular citrate accumulation are not yet fully understood. One of the less understood culture condi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01424

    authors: Odoni DI,van Gaal MP,Schonewille T,Tamayo-Ramos JA,Martins Dos Santos VAP,Suarez-Diez M,Schaap PJ

    更新日期:2017-08-02 00:00:00

  • Dual Repressive Function by Cip1, a Budding Yeast Analog of p21, in Cell-Cycle START Regulation.

    abstract::Cip1, a newly identified yeast analog of p21, is a Cln3-CDK inhibitor that negatively regulates cell-cycle START. However, its function remains poorly understood. In this study, we found that deletion of CLN3 did not result in bypass of G1-phase arrest caused by Cip1 overexpression. Cip1 depletion in cln3-null mutants...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01623

    authors: Li P,Liu X,Hao Z,Jia Y,Zhao X,Xie D,Dong J,Zeng F

    更新日期:2020-07-09 00:00:00

  • A New Whole Genome Culture-Independent Diagnostic Test (WG-CIDT) for Rapid Detection of Salmonella in Lettuce.

    abstract::The rapid detection of foodborne microbial pathogens contaminating fresh fruits and vegetables during the intervening period between harvest and consumption could revolutionize microbial quality assurance of food usually consumed raw and those with a limited shelf life. We have developed a sensitive, shotgun whole gen...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00602

    authors: Ogunremi D,Dupras AA,Naushad S,Gao R,Duceppe MO,Omidi K,Márquez IG,Huang H,Goodridge L,Lévesque RC,Hasan NA,Dadlani M,Dixon B,Magierowski S,Masson L

    更新日期:2020-04-17 00:00:00

  • Rhinovirus Biology, Antigenic Diversity, and Advancements in the Design of a Human Rhinovirus Vaccine.

    abstract::Human rhinovirus (HRV) remains a leading cause of several human diseases including the common cold. Despite considerable research over the last 60 years, development of an effective vaccine to HRV has been viewed by many as unfeasible due, in part, to the antigenic diversity of circulating HRVs in nature. Over 150 ant...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02412

    authors: Stobart CC,Nosek JM,Moore ML

    更新日期:2017-12-05 00:00:00

  • Anchors for effectors: subversion of phosphoinositide lipids by legionella.

    abstract::The facultative intracellular pathogen Legionella pneumophila replicates in free-living amoebae and macrophages within a distinct compartment, the "Legionella-containing vacuole" (LCV). LCV formation involves phosphoinositide (PI) glycerolipids, which are key factors controlling vesicle trafficking pathways and membra...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00091

    authors: Hilbi H,Weber S,Finsel I

    更新日期:2011-04-27 00:00:00

  • A Pilot Study of the Humoral Response Against the AntiSense Protein (ASP) in HIV-1-Infected Patients.

    abstract::The existence of an antisense Open Reading Frame (ORF) that encodes a putative AntiSense Protein (ASP) on the proviral genome of Human Immunodeficiency Virus type 1 (HIV-1) was a source of debate for 30 years. During the last years, some progresses have been made to characterize the cellular immune response against AS...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00020

    authors: Savoret J,Chazal N,Moles JP,Tuaillon E,Boufassa F,Meyer L,Lecuroux C,Lambotte O,Van De Perre P,Mesnard JM,Gross A

    更新日期:2020-01-24 00:00:00

  • Relationships between Cytokine Profiles and Signaling Pathways (IκB Kinase and p38 MAPK) in Parainfluenza Virus-Infected Lung Fibroblasts.

    abstract::Respiratory viruses such as parainfluenza virus (PIV) in individuals with certain genetic predispositions in early life are associated with the induction of wheezing, which can progress to the development of asthma. It has been suggested that aberrant production of various cytokines due to viral infection are associat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2010.00124

    authors: Yoshizumi M,Kimura H,Okayama Y,Nishina A,Noda M,Tsukagoshi H,Kozawa K,Kurabayashi M

    更新日期:2010-11-15 00:00:00

  • Gut Microbiome Contributes to Liver Fibrosis Impact on T Cell Receptor Immune Repertoire.

    abstract::Gut microbiota (GM) modifies the intrahepatic immune microenvironment, but the underlying mechanisms remain poorly understood. Liver fibrosis-associated imprinting is predicted to be reflected in GM. This study investigated the link between GM and the intrahepatic T cell receptor (TCR) immune repertoire (IR), and whet...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.571847

    authors: Liang Q,Zhang M,Hu Y,Zhang W,Zhu P,Chen Y,Xue P,Li Q,Wang K

    更新日期:2020-11-27 00:00:00

  • The Role of Strigolactone in the Cross-Talk Between Arabidopsis thaliana and the Endophytic Fungus Mucor sp.

    abstract::Over the last years the role of fungal endophytes in plant biology has been extensively studied. A number of species were shown to positively affect plant growth and fitness, thus attempts have been made to utilize these microorganisms in agriculture and phytoremediation. Plant-fungi symbiosis requires multiple metabo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00441

    authors: Rozpądek P,Domka AM,Nosek M,Ważny R,Jędrzejczyk RJ,Wiciarz M,Turnau K

    更新日期:2018-03-19 00:00:00

  • Inoculation Pneumonia Caused by Coagulase Negative Staphylococcus.

    abstract:Rationale:Although frequently retrieved in tracheal secretions of critically ill patients on mechanical ventilation, the existence of pneumonia caused by coagulase-negative staphylococci (CoNS) remains controversial. Objective:To assess whether Staphylococcus haemolyticus (S. haemolyticus) inoculated in mice's trachea...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02198

    authors: Shi MM,Monsel A,Rouby JJ,Xu YP,Zhu YG,Qu JM

    更新日期:2019-10-04 00:00:00

  • Atorvastatin Reduces the Survival of Candida albicans-Infected BALB/c Mice.

    abstract::Several antimicrobial and immunosuppressive effects have been attributed to the statins class of antihyperlipidemia drugs. Several studies have also indicated clinical benefits for the use of statins during the management of infections and sepsis. To assess whether the immunosuppressive effects of statins outweigh the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01474

    authors: Rahal EA,Constantin WN,Zeidan N,Abdelnoor AM

    更新日期:2015-12-22 00:00:00

  • Novel Butane-Oxidizing Bacteria and Diversity of bmoX Genes in Puguang Gas Field.

    abstract::To investigate the diversity of butane-oxidizing bacteria in soils contaminated by long-term light hydrocarbon microseepage and the influence of butane on the soil microbial community, a quantitative study and identification of butane-oxidizing bacteria (BOB) in soils at the Puguang gas field were performed by DNA-bas...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01576

    authors: Deng Y,Deng C,Yang J,Li B,Wang E,Yuan H

    更新日期:2018-07-17 00:00:00

  • First Insights Into Bacterial Gastrointestinal Tract Communities of the Eurasian Beaver (Castor fiber).

    abstract::The Eurasian or European beaver (Castor fiber) is the second-largest living rodent after the capybara. It is a semi-aquatic animal known for building dams and lodges. They strictly feed on lignocellulose-rich plants and correspondingly harbor cellulolytic microbial communities in their digestive tract. In this study, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01646

    authors: Pratama R,Schneider D,Böer T,Daniel R

    更新日期:2019-07-25 00:00:00

  • Comparison among the Quantification of Bacterial Pathogens by qPCR, dPCR, and Cultural Methods.

    abstract::The demand for rapid methods for the quantification of pathogens is increasing. Among these methods, those based on nucleic acids amplification (quantitative PCRs) are the most widespread worldwide. Together with the qPCR, a new approach named digital PCR (dPCR), has rapidly gained importance. The aim of our study was...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01174

    authors: Ricchi M,Bertasio C,Boniotti MB,Vicari N,Russo S,Tilola M,Bellotti MA,Bertasi B

    更新日期:2017-06-28 00:00:00