Rapid Purification and Characterization of Mutant Origin Recognition Complexes in Saccharomyces cerevisiae.

abstract：:Type three secretion system (T3SS) is essential for Ralstonia solanacearum to cause disease in host plants and we previously screened AroG1 as a candidate with impact on the T3SS expression. Here, we focused on two putative DAHP synthases of AroG1 and AroG2, which control the first step of the shikimate pathway, a com...

journal_title：Frontiers in microbiology

authors： Zhang W,Li J,Shi X,Hikichi Y,Zhang Y,Ohnishi K

更新日期：2019-02-12 00:00:00

abstract：:Schizophyllum commune is a filamentous basidiomycete causing white-rot in many wood species with the help of a broad range of enzymes including multicopper oxidases such as laccases and laccase-like oxidases. Since these enzymes exhibit a broad substrate range, their ability to oxidatively degrade black slate was inve...

journal_title：Frontiers in microbiology

authors： Kirtzel J,Madhavan S,Wielsch N,Blinne A,Hupfer Y,Linde J,Krause K,Svatoš A,Kothe E

更新日期：2018-10-24 00:00:00

abstract：:Dissimilatory metal-reducing bacteria are widespread in terrestrial ecosystems, especially in anaerobic soils and sediments. Thermodynamically, dissimilatory metal reduction is more favorable than sulfate reduction and methanogenesis but less favorable than denitrification and aerobic respiration. It is critical to un...

journal_title：Frontiers in microbiology

authors： Friedman ES,McPhillips LE,Werner JJ,Poole AC,Ley RE,Walter MT,Angenent LT

更新日期：2016-01-11 00:00:00

abstract：:While vegetation has intensively been surveyed on mountain summits, limited knowledge exists about the diversity and community structure of soil biota. Here, we study how climatic variables, vegetation, parent material, soil properties, and slope aspect affect the soil microbiome on 10 GLORIA (Global Observation Resea...

journal_title：Frontiers in microbiology

authors： Adamczyk M,Hagedorn F,Wipf S,Donhauser J,Vittoz P,Rixen C,Frossard A,Theurillat JP,Frey B

更新日期：2019-05-15 00:00:00

abstract：:While multicellular magnetotactic prokaryotes (MMPs) are ubiquitous in marine environments, the diversity of MMPs in sediments of coral reef ecosystems has rarely been reported. In this study, we made an investigation on the diversity and characteristics of MMPs in sediments at 11 stations in coral reef habitats of th...

journal_title：Frontiers in microbiology

authors： Teng Z,Zhang Y,Zhang W,Pan H,Xu J,Huang H,Xiao T,Wu LF

更新日期：2018-09-11 00:00:00

abstract：:Probiotic bacteria have been associated with various health benefits and included in overwhelming number of foods. Today, probiotic supplements are consumed with increasing regularity and record a rapidly growing economic value. With billions of heterogeneous populations of probiotics per serving, probiotic supplement...

journal_title：Frontiers in microbiology

authors： Wang Y,Jiang Y,Deng Y,Yi C,Wang Y,Ding M,Liu J,Jin X,Shen L,He Y,Wu X,Chen X,Sun C,Zheng M,Zhang R,Ye H,An H,Wong A

更新日期：2020-02-28 00:00:00

abstract：:Food-associated microbes can reach the gut as viable cells and interact with the human host providing potential health benefits. In this study, we evaluated the impact on cell viability and the adhesion ability of 22 Lactobacillus plantarum strains, mainly isolated from fermented foods, on a Normal-derived Colon Mucos...

journal_title：Frontiers in microbiology

authors： Garcia-Gonzalez N,Prete R,Battista N,Corsetti A

更新日期：2018-10-08 00:00:00

abstract：:The translation of enterovirus 71 (EV71) is mediated by an internal ribosome entry site (IRES)-dependent manner. EV71 IRES comprises five highly structured domains (domains II-VI) in the 5'-untranslated region of the viral mRNA. A conserved AUG triplet residing in domain VI is proposed to be the ribosome entry site. I...

journal_title：Frontiers in microbiology

authors： Su YS,Tsai AH,Ho YF,Huang SY,Liu YC,Hwang LH

更新日期：2018-06-19 00:00:00

abstract：:To investigate the diversity of butane-oxidizing bacteria in soils contaminated by long-term light hydrocarbon microseepage and the influence of butane on the soil microbial community, a quantitative study and identification of butane-oxidizing bacteria (BOB) in soils at the Puguang gas field were performed by DNA-bas...

journal_title：Frontiers in microbiology

authors： Deng Y,Deng C,Yang J,Li B,Wang E,Yuan H

更新日期：2018-07-17 00:00:00

abstract：:Climate, soil, and grape varieties are the primary characteristics of terroir and lead to the definition of various appellations of origin. However, the microbiota associated with grapes are also affected by these conditions and can leave a footprint in a wine that will be part of the characteristics of terroir. Thus,...

journal_title：Frontiers in microbiology

authors： Padilla B,García-Fernández D,González B,Izidoro I,Esteve-Zarzoso B,Beltran G,Mas A

更新日期：2016-06-15 00:00:00

abstract：:Yersinia pestis, responsible for causing fulminant plague, has evolved clonally from the enteric pathogen, Y. pseudotuberculosis, which in contrast, causes a relatively benign enteric illness. An ~97% nucleotide identity over 75% of their shared protein coding genes is maintained between these two pathogens, leaving m...

journal_title：Frontiers in microbiology

authors： Martínez-Chavarría LC,Vadyvaloo V

更新日期：2015-09-17 00:00:00

abstract：:Colonization of the root surface, or rhizoplane, is one of the first steps for soil-borne bacteria to become established in the plant microbiome. However, the relative contributions of processes, such as bacterial attachment and proliferation is not well characterized, and this limits our ability to comprehend the com...

journal_title：Frontiers in microbiology

authors： Carroll D,Holden N,Gifford ML,Dupuy LX

更新日期：2020-09-25 00:00:00

abstract：:Streptococcus suis serotype 2 (SS2) is an emerging human pathogen worldwide. A large outbreak occurred in the summer of 2005 in China. Serum samples from this outbreak revealed that levels of the main proinflammatory cytokines were significantly higher in patients with streptococcal toxic-shock-like syndrome (STSLS) t...

journal_title：Frontiers in microbiology

authors： Bi L,Pian Y,Chen S,Ren Z,Liu P,Lv Q,Zheng Y,Zhang S,Hao H,Yuan Y,Jiang Y

更新日期：2015-06-26 00:00:00

abstract：:Microalgae and probiotics such as Bifidobacterium and Lactobacillus genera are associated with human beneficial effects. The aim of this study was to evaluate the activity of Chlorella sorokiniana on Bifidobacterium longum and Lactobacillus plantarum viability in a dairy product (flan) and its microbial effect against...

journal_title：Frontiers in microbiology

authors： Cantú-Bernal S,Domínguez-Gámez M,Medina-Peraza I,Aros-Uzarraga E,Ontiveros N,Flores-Mendoza L,Gomez-Flores R,Tamez-Guerra P,González-Ochoa G

更新日期：2020-05-12 00:00:00

abstract：:In filamentous fungi such as Fusarium graminearum, disruption of multiple genes of interest in the same strain (e.g., to test for redundant gene function) is a difficult task due to the limited availability of reliable selection markers. We have created a series of transformation vectors that allow antibiotic-based se...

journal_title：Frontiers in microbiology

authors： Twaruschek K,Spörhase P,Michlmayr H,Wiesenberger G,Adam G

更新日期：2018-09-11 00:00:00

abstract：:Azole-resistant (azole-R) Aspergillus is an increasing challenge worldwide. Patients with cystic fibrosis (CF) are at risk of Aspergillus colonization and disease due to a favorable lung environment for microorganisms. We performed a nationwide study in 2018 of azole-non-susceptible Aspergillus in CF patients and comp...

journal_title：Frontiers in microbiology

authors： Risum M,Hare RK,Gertsen JB,Kristensen L,Johansen HK,Helweg-Larsen J,Abou-Chakra N,Pressler T,Skov M,Jensen-Fangel S,Arendrup MC

更新日期：2020-08-13 00:00:00

abstract：:Shigella is an important human food-borne zoonosis bacterial pathogen, and can cause clinically severe diarrhea. There is an urgent need to develop a specific, sensitive, and rapid methodology for detection of this pathogen. In this study, loop-mediated isothermal amplification (LAMP) combined with magnetic immunocapt...

journal_title：Frontiers in microbiology

authors： Zhang L,Wei Q,Han Q,Chen Q,Tai W,Zhang J,Song Y,Xia X

更新日期：2018-02-06 00:00:00

abstract：:Bifidobacterium species are used as probiotics to provide beneficial effects to humans. These effects are specific to some species or subspecies of Bifidobacterium. However, some Bifidobacterium species or subspecies are not distinguished because similarity of 16S rRNA and housekeeping gene sequences within Bifidobact...

journal_title：Frontiers in microbiology

authors： Kim HB,Kim E,Yang SM,Lee S,Kim MJ,Kim HY

更新日期：2020-08-28 00:00:00

abstract：:The increased use of molecular identification methods and mass spectrometry has revealed that Acinetobacter spp. of the A. baumannii (Ab) group other than A. baumannii are increasingly being recovered from human samples and may pose a health challenge if neglected. In this study 76 isolates of 5 species within the Ab ...

journal_title：Frontiers in microbiology

authors： Cosgaya C,Ratia C,Marí-Almirall M,Rubio L,Higgins PG,Seifert H,Roca I,Vila J

更新日期：2019-10-24 00:00:00

abstract：:Human breast milk is widely recognized as the best source of nutrients for healthy growth and development of infants; it contains a diverse microbiota. Here, we characterized the diversity of the microbiota in the breast milk of East Asian women and assessed whether delivery mode influenced the microbiota in the milk ...

journal_title：Frontiers in microbiology

authors： Li SW,Watanabe K,Hsu CC,Chao SH,Yang ZH,Lin YJ,Chen CC,Cao YM,Huang HC,Chang CH,Tsai YC

更新日期：2017-05-30 00:00:00

abstract：:Microorganisms have a wonderful ability to adapt rapidly to new or altered environmental conditions. Enzymes are the basis of metabolism in all living organisms and, therefore, enzyme adaptation plays a crucial role in the adaptation of microorganisms. Comparisons of homology and parallel beneficial mutations in an en...

journal_title：Frontiers in microbiology

authors： Suenaga H

更新日期：2015-09-22 00:00:00

abstract：:Host-cell expression of the ezrin protein is required for CXCR4 (X4)-tropic HIV-1 infection. Ezrin function is regulated by phosphorylation at threonine-567. This study investigates the role of ezrin phosphorylation in HIV-1 infection and virion release. We analyzed the effects of ezrin mutations involving substitutio...

journal_title：Frontiers in microbiology

authors： Kamiyama H,Izumida M,Umemura Y,Hayashi H,Matsuyama T,Kubo Y

更新日期：2018-08-27 00:00:00

abstract：:Human endogenous retroviruses (ERVs) have been found to be associated with different diseases, e.g., multiple sclerosis (MS). Most human ERVs integrated in our genome are not competent to replicate and these sequences are presumably silent. However, transcription of human ERVs can be reactivated, e.g., by hypoxia. Int...

journal_title：Frontiers in microbiology

authors： Brütting C,Narasimhan H,Hoffmann F,Kornhuber ME,Staege MS,Emmer A

更新日期：2018-02-21 00:00:00

abstract：:Human urine was considered sterile for a long time. However, 416 species have been previously cultured, including only 40 anaerobic species. Here, we used culturomics, particularly those targeting anaerobes, to better understand the urinary microbiota. By testing 435 urine samples, we isolated 450 different bacterial ...

journal_title：Frontiers in microbiology

authors： Dubourg G,Morand A,Mekhalif F,Godefroy R,Corthier A,Yacouba A,Diakite A,Cornu F,Cresci M,Brahimi S,Caputo A,Lechevallier E,Tsimaratos M,Moal V,Lagier JC,Raoult D

更新日期：2020-10-16 00:00:00

abstract：:As Cryptococcus neoformans mother cells generationally age, their cell walls become thicker and cell-wall associated virulence factors are upregulated. Antiphagocytic protein 1 (App1), and laccase enzymes (Lac1 and Lac2) are virulence factors known to contribute to virulence of C. neoformans during infection through i...

journal_title：Frontiers in microbiology

authors： Orner EP,Bhattacharya S,Kalenja K,Hayden D,Del Poeta M,Fries BC

更新日期：2019-11-07 00:00:00

abstract：:Helicobacter pylori (H. pylori) is well-known to be involved in gastric carcinogenesis, associated with deregulation of cell proliferation and epigenetic changes in cancer-related genes. H. pylori infection is largely acquired during childhood, persisting long-term in about half of infected individuals, a subset of wh...

journal_title：Frontiers in microbiology

authors： George S,Lucero Y,Torres JP,Lagomarcino AJ,O'Ryan M

更新日期：2020-02-12 00:00:00

abstract：:The Formyl-peptide receptor-2 (FPR2) is a seven transmembrane G protein-coupled receptor, which plays an important role in sensing of bacteria and modulation of immune responses. FPR2 is also used by viruses for their own profit. Annexin A1, one of the multiple ligands of FPR2, is incorporated in the budding virus mem...

journal_title：Frontiers in microbiology

authors： Alessi MC,Cenac N,Si-Tahar M,Riteau B

更新日期：2017-09-05 00:00:00

abstract：:The pathogenic bacterium Helicobacter pylori is genetically highly diverse and a major risk factor for the development of peptic ulcer disease and gastric adenocarcinoma in humans. During evolution, H. pylori has acquired multiple type IV secretion systems (T4SSs), and then adapted for various purposes. These T4SSs re...

journal_title：Frontiers in microbiology

authors： Fischer W,Tegtmeyer N,Stingl K,Backert S

更新日期：2020-07-10 00:00:00

abstract：:A library of 14 minimally cytotoxic diterpenoid-like compounds (CC50 > 70 μM on HepG2 human liver cells) was screened against Mycobacterium smegmatis, Staphylococcus aureus, and Escherichia coli to determine antimicrobial activity. Some compounds with a phenethyl alcohol (PE) core substituted with a β-cyclocitral deri...

journal_title：Frontiers in microbiology

authors： Crusco A,Baptista R,Bhowmick S,Beckmann M,Mur LAJ,Westwell AD,Hoffmann KF

更新日期：2019-06-25 00:00:00

abstract：:Helicobacter pylori does not encode the classical DsbA/DsbB oxidoreductases that are crucial for oxidative folding of extracytoplasmic proteins. Instead, this microorganism encodes an untypical two proteins playing a role in disulfide bond formation - periplasmic HP0231, which structure resembles that of EcDsbC/DsbG, ...

journal_title：Frontiers in microbiology

authors： Bocian-Ostrzycka KM,Łasica AM,Dunin-Horkawicz S,Grzeszczuk MJ,Drabik K,Dobosz AM,Godlewska R,Nowak E,Collet JF,Jagusztyn-Krynicka EK

更新日期：2015-10-08 00:00:00