Cell Wall-Associated Virulence Factors Contribute to Increased Resilience of Old Cryptococcus neoformans Cells.

Abstract:

:As Cryptococcus neoformans mother cells generationally age, their cell walls become thicker and cell-wall associated virulence factors are upregulated. Antiphagocytic protein 1 (App1), and laccase enzymes (Lac1 and Lac2) are virulence factors known to contribute to virulence of C. neoformans during infection through inhibition of phagocytic uptake and melanization. Here we show that these cell-wall-associated proteins are not only significantly upregulated in old C. neoformans cells, but also that their upregulation likely contributes to the increased resistance to antifungal and host-mediated killing during infection and to the subsequent accumulation of old cells. We found that old cells melanize to a greater extent than younger cells and as a consequence, old melanized cells are more resistant to killing by amphotericin B compared to young melanized cells. A decrease in melanization of old lacΔ mutants lead to a decrease in old-cell resilience, indicating that age-related melanization is contributing to the overall resilience of older cells and is being mediated by laccase genes. Additionally, we found that older cells are more resistant to macrophage phagocytosis, but this resistance is lost when APP1 is knocked out, indicating that upregulation of APP1 in older cells is in part responsible for their increased resistance to phagocytosis by macrophages. Finally, infections with old cells in the Galleria mellonella model support our conclusions, as loss of the APP1, LAC1, and LAC2 gene ablates the enhanced virulence of old cells, indicating their importance in age-dependent resilience.

journal_name

Front Microbiol

authors

Orner EP,Bhattacharya S,Kalenja K,Hayden D,Del Poeta M,Fries BC

doi

10.3389/fmicb.2019.02513

subject

Has Abstract

pub_date

2019-11-07 00:00:00

pages

2513

issn

1664-302X

journal_volume

10

pub_type

杂志文章
  • Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein From Halobacillus andaensis and Functional Analysis of Its Conserved Arginine/Aspartate Residues.

    abstract::Arginine-aspartate-aspartate (RDD) family, representing a category of transmembrane proteins containing one highly conserved arginine and two highly conserved aspartates, has been functionally uncharacterized as yet. Here we present the characterization of a member of this family designated RDD from the moderate halop...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00807

    authors: Shao L,Abdel-Motaal H,Chen J,Chen H,Xu T,Meng L,Zhang Z,Meng F,Jiang J

    更新日期:2018-04-25 00:00:00

  • Biosurveillance of avian influenza and Newcastle disease viruses in the Barda region of Azerbaijan using real time RT-PCR and hemagglutination inhibition.

    abstract::The Azerbaijan State Veterinary Control Service (SVCS) has conducted active serological surveillance for avian influenza (AI) in poultry since 2006, when the first outbreak of AI H5N1 occurred in Azerbaijan. Samples are collected from September to May annually and tested using a hemagglutination inhibition (HI) assay ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01128

    authors: Zeynalova S,Guliyev F,Vatani M,Abbasov B

    更新日期:2015-11-06 00:00:00

  • PNA Length Restriction of Antibacterial Activity of Peptide-PNA Conjugates in Escherichia coli Through Effects of the Inner Membrane.

    abstract::Peptide Nucleic Acid (PNA)-peptide conjugates targeting essential bacterial genes are showing promise as antisense antimicrobials in drug discovery. Optimization has focused on selection of target genes and exact localization around the ribosome binding site, but surprisingly a length optimum around 10-12 nucleobases ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01032

    authors: Goltermann L,Yavari N,Zhang M,Ghosal A,Nielsen PE

    更新日期:2019-05-24 00:00:00

  • Modulation of Plant Defense System in Response to Microbial Interactions.

    abstract::At different stages throughout their life cycle, plants often encounter several pathogenic microbes that challenge plant growth and development. The sophisticated innate plant immune system prevents the growth of harmful microbes via two interconnected defense strategies based on pathogen perception. These strategies ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.01298

    authors: Nishad R,Ahmed T,Rahman VJ,Kareem A

    更新日期:2020-07-03 00:00:00

  • Algae-based oral recombinant vaccines.

    abstract::Recombinant subunit vaccines are some of the safest and most effective vaccines available, but their high cost and the requirement of advanced medical infrastructure for administration make them impractical for many developing world diseases. Plant-based vaccines have shifted that paradigm by paving the way for recomb...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00060

    authors: Specht EA,Mayfield SP

    更新日期:2014-02-17 00:00:00

  • Novel Extracellular Electron Transfer Channels in a Gram-Positive Thermophilic Bacterium.

    abstract::Biogenic transformation of Fe minerals, associated with extracellular electron transfer (EET), allows microorganisms to exploit high-potential refractory electron acceptors for energy generation. EET-capable thermophiles are dominated by hyperthermophilic archaea and Gram-positive bacteria. Information on their EET pa...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.597818

    authors: Gavrilov SN,Zavarzina DG,Elizarov IM,Tikhonova TV,Dergousova NI,Popov VO,Lloyd JR,Knight D,El-Naggar MY,Pirbadian S,Leung KM,Robb FT,Zakhartsev MV,Bretschger O,Bonch-Osmolovskaya EA

    更新日期:2021-01-11 00:00:00

  • Catabolism of Alkylphenols in Rhodococcus via a Meta-Cleavage Pathway Associated With Genomic Islands.

    abstract::The bacterial catabolism of aromatic compounds has considerable promise to convert lignin depolymerization products to commercial chemicals. Alkylphenols are a key class of depolymerization products whose catabolism is not well-elucidated. We isolated Rhodococcus rhodochrous EP4 on 4-ethylphenol and applied genomic an...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01862

    authors: Levy-Booth DJ,Fetherolf MM,Stewart GR,Liu J,Eltis LD,Mohn WW

    更新日期:2019-08-20 00:00:00

  • Growth and membrane fluidity of food-borne pathogen Listeria monocytogenes in the presence of weak acid preservatives and hydrochloric acid.

    abstract::This study addresses a major issue in microbial food safety, the elucidation of correlations between acid stress and changes in membrane fluidity of the pathogen Listeria monocytogenes. In order to assess the possible role that membrane fluidity changes play in L. monocytogenes tolerance to antimicrobial acids (acetic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00152

    authors: Diakogiannis I,Berberi A,Siapi E,Arkoudi-Vafea A,Giannopoulou L,Mastronicolis SK

    更新日期:2013-06-14 00:00:00

  • Molecular Detection of Azole-Resistant Aspergillus fumigatus in Clinical Samples.

    abstract::Aspergillus diseases are often caused by Aspergillus fumigatus. Azoles are the mainstay of therapy, but the management of aspergillosis is hampered by the emergence of azole resistance. Rapid detection of azole resistance might benefit treatment outcome by early treatment modifications. However, the yield of fungal cu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.00515

    authors: Buil JB,Zoll J,Verweij PE,Melchers WJG

    更新日期:2018-03-21 00:00:00

  • First Report of Vibrio tubiashii Associated with a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile.

    abstract::The VPAP30 strain was isolated as the highly predominant bacteria from an episode of massive larval mortality occurring in a commercial culture of the Chilean scallop Argopecten purpuratus. The main aims of this study were, to characterize and identify the pathogenic strain using biochemical and molecular methods to d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,收录出版

    doi:10.3389/fmicb.2016.01473

    authors: Rojas R,Miranda CD,Santander J,Romero J

    更新日期:2016-09-20 00:00:00

  • Bacterial Microcompartment-Dependent 1,2-Propanediol Utilization Stimulates Anaerobic Growth of Listeria monocytogenes EGDe.

    abstract::Bacterial microcompartments (BMCs) are proteinaceous organelles that optimize specific metabolic pathways referred to as metabolosomes involving transient production of toxic volatile metabolites such as aldehydes. Previous bioinformatics analysis predicted the presence of BMCs in 23 bacterial phyla including foodborn...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02660

    authors: Zeng Z,Smid EJ,Boeren S,Notebaart RA,Abee T

    更新日期:2019-11-15 00:00:00

  • Discovery of Novel Biosynthetic Gene Cluster Diversity From a Soil Metagenomic Library.

    abstract::Soil microorganisms historically have been a rich resource for natural product discovery, yet the majority of these microbes remain uncultivated and their biosynthetic capacity is left underexplored. To identify the biosynthetic potential of soil microorganisms using a culture-independent approach, we constructed a la...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.585398

    authors: Santana-Pereira ALR,Sandoval-Powers M,Monsma S,Zhou J,Santos SR,Mead DA,Liles MR

    更新日期:2020-12-07 00:00:00

  • Colonization and Internalization of Salmonella enterica and Its Prevalence in Cucumber Plants.

    abstract::Consumption of cucumbers (Cucumis sativus var. sativus) has been linked to several foodborne outbreaks involving Salmonella enterica. The purpose of this work was to investigate the efficiency of colonization and internalization of S. enterica into cucumber plants by various routes of contamination. Produce-associated...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01135

    authors: Burris KP,Simmons OD,Webb HM,Deese LM,Moore RG,Jaykus LA,Zheng J,Reed E,Ferreira CM,Brown EW,Bell RL

    更新日期:2020-05-29 00:00:00

  • PGE2 Augments Inflammasome Activation and M1 Polarization in Macrophages Infected With Salmonella Typhimurium and Yersinia enterocolitica.

    abstract::Eicosanoids are cellular metabolites, which shape the immune response, including inflammatory processes in macrophages. The effects of these lipid mediators on inflammation and bacterial pathogenesis are not clearly understood. Certain eicosanoids are suspected to act as molecular sensors for the recruitment of neutro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02447

    authors: Sheppe AEF,Kummari E,Walker A,Richards A,Hui WW,Lee JH,Mangum L,Borazjani A,Ross MK,Edelmann MJ

    更新日期:2018-10-31 00:00:00

  • New Plasmodium vivax Genomes From the China-Myanmar Border.

    abstract::Plasmodium vivax is increasingly the dominant species of malaria in the Greater Mekong Subregion (GMS), which is pursuing regional malaria elimination. P. vivax lineages in the GMS are poorly characterized. Currently, P. vivax reference genomes are scarce due to difficulties in culturing the parasite and lack of high-...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01930

    authors: Brashear AM,Huckaby AC,Fan Q,Dillard LJ,Hu Y,Li Y,Zhao Y,Wang Z,Cao Y,Miao J,Guler JL,Cui L

    更新日期:2020-08-11 00:00:00

  • Microbial carbon mineralization in tropical lowland and montane forest soils of Peru.

    abstract::Climate change is affecting the amount and complexity of plant inputs to tropical forest soils. This is likely to influence the carbon (C) balance of these ecosystems by altering decomposition processes e.g., "positive priming effects" that accelerate soil organic matter mineralization. However, the mechanisms determi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00720

    authors: Whitaker J,Ostle N,McNamara NP,Nottingham AT,Stott AW,Bardgett RD,Salinas N,Ccahuana AJ,Meir P

    更新日期:2014-12-18 00:00:00

  • Explication of the Potential of 2-Hydroxy-4-Methoxybenzaldehyde in Hampering Uropathogenic Proteus mirabilis Crystalline Biofilm and Virulence.

    abstract::Proteus mirabilis is an important etiological agent of catheter-associated urinary tract infections (CAUTIs) owing to its efficient crystalline biofilm formation and virulence enzyme production. Hence, the present study explicated the antibiofilm and antivirulence efficacies of 2-hydroxy-4-methoxybenzaldehyde (HMB) ag...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02804

    authors: Durgadevi R,Abirami G,Alexpandi R,Nandhini K,Kumar P,Prakash S,Veera Ravi A

    更新日期:2019-12-10 00:00:00

  • The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments.

    abstract::The sheep intestinal tract is characterized by a diverse microbial ecosystem that is vital for the host to digest diet material. The importance of gut microbiota (GM) of animals has also been widely acknowledged because of its pivotal roles in the health and well-being of animals. However, there are no relevant studie...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00032

    authors: Zhang H,Shao M,Huang H,Wang S,Ma L,Wang H,Hu L,Wei K,Zhu R

    更新日期:2018-01-31 00:00:00

  • Emerging Trends in Biological Treatment of Wastewater From Unconventional Oil and Gas Extraction.

    abstract::Unconventional oil and gas exploration generates an enormous quantity of wastewater, commonly referred to as flowback and produced water (FPW). Limited freshwater resources and stringent disposal regulations have provided impetus for FPW reuse. Organic and inorganic compounds released from the shale/brine formation, m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.569019

    authors: Acharya SM,Chakraborty R,Tringe SG

    更新日期:2020-09-09 00:00:00

  • Comparative Analysis of Gut Microbiota Changes in Père David's Deer Populations in Beijing Milu Park and Shishou, Hubei Province in China.

    abstract::This study used 16S rRNA high-throughput sequencing technology to examine the differences in gut microbiota between the Père David's deer populations in the Beijing and Shishou areas of China in order to understand the effects of ex situ conservation on the intestinal microflora in the Père David's deer. Results: On t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01258

    authors: Zhang M,Shi M,Fan M,Xu S,Li Y,Zhang T,Cha M,Liu Y,Guo X,Chen Q,Li Y,Zhang S,Hu D,Liu S

    更新日期:2018-06-12 00:00:00

  • Biophysical Characterization of Membrane Phase Transition Profiles for the Discrimination of Outer Membrane Vesicles (OMVs) From Escherichia coli Grown at Different Temperatures.

    abstract::Dynamic Light Scattering (DLS), Small Angle X-ray Scattering (SAXS) and Transmission Electron Microscopy (TEM) are physical techniques widely employed to characterize the morphology and the structure of vesicles such as liposomes or human extracellular vesicles (exosomes). Bacterial extracellular vesicles are similar ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00290

    authors: Sarra A,Celluzzi A,Bruno SP,Ricci C,Sennato S,Ortore MG,Casciardi S,Del Chierico F,Postorino P,Bordi F,Masotti A

    更新日期:2020-02-27 00:00:00

  • Expanding the Current Knowledge About the Role of Interleukin-10 to Major Concerning Bacteria.

    abstract::Interleukin-10 (IL-10) is one of the most important anti-inflammatory cytokine produced during bacterial infection. Two related phenomena explain the importance of IL-10 production in this context: first, the wide range of cells able to produce this cytokine and second, the wide effects that it causes on target cells....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02047

    authors: Peñaloza HF,Noguera LP,Riedel CA,Bueno SM

    更新日期:2018-09-18 00:00:00

  • Bacillus cereus cell response upon exposure to acid environment: toward the identification of potential biomarkers.

    abstract::Microorganisms are able to adapt to different environments and evolve rapidly, allowing them to cope with their new environments. Such adaptive response and associated protections toward other lethal stresses, is a crucial survival strategy for a wide spectrum of microorganisms, including food spoilage bacteria, patho...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2013.00284

    authors: Desriac N,Broussolle V,Postollec F,Mathot AG,Sohier D,Coroller L,Leguerinel I

    更新日期:2013-10-02 00:00:00

  • Importance of Promyelocytic Leukema Protein (PML) for Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication.

    abstract::Many DNA virus replication-related proteins are associated with promyelocytic leukemia protein (PML), a component of nuclear domain 10 (ND10), which has been investigated for its potential involvement in viral replication. In the case of Kaposi's sarcoma-associated herpesvirus (KSHV) lytic gene products, K8 (K-bZIP), ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02324

    authors: Hossain MG,Ohsaki E,Honda T,Ueda K

    更新日期:2018-10-08 00:00:00

  • Sulfate-Reducing Bacteria as an Effective Tool for Sustainable Acid Mine Bioremediation.

    abstract::Mining industries produce vast waste streams that pose severe environmental pollution challenge. Conventional techniques of treatment are usually inefficient and unsustainable. Biological technique employing the use of microorganisms is a competitive alternative to treat mine wastes and recover toxic heavy metals. Mic...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.01986

    authors: Ayangbenro AS,Olanrewaju OS,Babalola OO

    更新日期:2018-08-22 00:00:00

  • Identification of the Genes Required for the Culture of Liberibacter crescens, the Closest Cultured Relative of the Liberibacter Plant Pathogens.

    abstract::Here Tn5 random transposon mutagenesis was used to identify the essential elements for culturing Liberibacter crescens BT-1 that can serve as antimicrobial targets for the closely related pathogens of citrus, Candidatus Liberibacter asiaticus (Las) and tomato and potato, Candidatus Liberibacter solanacearum (Lso). In ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00547

    authors: Lai KK,Davis-Richardson AG,Dias R,Triplett EW

    更新日期:2016-04-20 00:00:00

  • The symbiont side of symbiosis: do microbes really benefit?

    abstract::Microbial associations are integral to all eukaryotes. Mutualism, the interaction of two species for the benefit of both, is an important aspect of microbial associations, with evidence that multicellular organisms in particular benefit from microbes. However, the microbe's perspective has largely been ignored, and it...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00510

    authors: Garcia JR,Gerardo NM

    更新日期:2014-09-26 00:00:00

  • Biocontrol Traits Correlate With Resistance to Predation by Protists in Soil Pseudomonads.

    abstract::Root-colonizing bacteria can support plant growth and help fend off pathogens. It is clear that such bacteria benefit from plant-derived carbon, but it remains ambiguous why they invest in plant-beneficial traits. We suggest that selection via protist predation contributes to recruitment of plant-beneficial traits in ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.614194

    authors: Amacker N,Gao Z,Agaras BC,Latz E,Kowalchuk GA,Valverde CF,Jousset A,Weidner S

    更新日期:2020-12-15 00:00:00

  • Long term seasonal dynamics of synechococcus population structure in the gulf of aqaba, northern red sea.

    abstract::Spatial patterns of marine Synechococcus diversity across ocean domains have been reported on extensively. However, much less is known of seasonal and multiannual patterns of change in Synechococcus community composition. Here we report on the genotypic diversity of Synechococcus populations in the Gulf of Aqaba, Nort...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00131

    authors: Post AF,Penno S,Zandbank K,Paytan A,Huse SM,Welch DM

    更新日期:2011-06-20 00:00:00

  • Effects of Polymorphisms in the SjSP-13 Gene of Schistosoma japonicum on Its Diagnostic Efficacy and Immunogenicity.

    abstract::Schistosomiasis japonica is one of the most prevalent parasitic diseases in China. The scarcity of effective diagnostic tools is a major factor that contributes to the high prevalence of schistosomiasis japonica. SjSP-13 is a promising serological diagnostic biomarker of the disease. However, it is unclear whether pol...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01695

    authors: Xu X,Cui X,Zhu L,Li Z,Zhang Y,Ma L,Pan W

    更新日期:2018-07-25 00:00:00