Framework for Quantification of the Dynamics of Root Colonization by Pseudomonas fluorescens Isolate SBW25.

Abstract:

:Colonization of the root surface, or rhizoplane, is one of the first steps for soil-borne bacteria to become established in the plant microbiome. However, the relative contributions of processes, such as bacterial attachment and proliferation is not well characterized, and this limits our ability to comprehend the complex dynamics of microbial communities in the rhizosphere. The work presented here addresses this knowledge gap. A model system was developed to acquire quantitative data on the colonization process of lettuce (Lactuca sativa L. cultivar. All Year Round) roots by Pseudomonas fluorescens isolate SBW25. A theoretical framework is proposed to calculate attachment rate and quantify the relative contribution of bacterial attachment to colonization. This allows the assessment of attachment rates on the root surface beyond the short time period during which it can be quantified experimentally. All techniques proposed are generic and similar analyses could be applied to study various combinations of plants and bacteria, or to assess competition between species. In the future this could allow for selection of microbial traits that improve early colonization and maintenance of targeted isolates in cropping systems, with potential applications for the development of biological fertilizers.

journal_name

Front Microbiol

authors

Carroll D,Holden N,Gifford ML,Dupuy LX

doi

10.3389/fmicb.2020.585443

subject

Has Abstract

pub_date

2020-09-25 00:00:00

pages

585443

issn

1664-302X

journal_volume

11

pub_type

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