Aspergillus niger Secretes Citrate to Increase Iron Bioavailability.

Abstract:

:Aspergillus niger has an innate ability to secrete various organic acids, including citrate. The conditions required for A. niger citrate overproduction are well described, but the physiological reasons underlying extracellular citrate accumulation are not yet fully understood. One of the less understood culture conditions is the requirement of growth-limiting iron concentrations. While this has been attributed to iron-dependent citrate metabolizing enzymes, this straightforward relationship does not always hold true. Here, we show that an increase in citrate secretion under iron limited conditions is a physiological response consistent with a role of citrate as A. niger iron siderophore. We found that A. niger citrate secretion increases with decreasing amounts of iron added to the culture medium and, in contrast to previous findings, this response is independent of the nitrogen source. Differential transcriptomics analyses of the two A. niger mutants NW305 (gluconate non-producer) and NW186 (gluconate and oxalate non-producer) revealed up-regulation of the citrate biosynthesis gene citA under iron limited conditions compared to iron replete conditions. In addition, we show that A. niger can utilize Fe(III) citrate as iron source. Finally, we discuss our findings in the general context of the pH-dependency of A. niger organic acid production, offering an explanation, besides competition, for why A. niger organic acid production is a sequential process influenced by the external pH of the culture medium.

journal_name

Front Microbiol

authors

Odoni DI,van Gaal MP,Schonewille T,Tamayo-Ramos JA,Martins Dos Santos VAP,Suarez-Diez M,Schaap PJ

doi

10.3389/fmicb.2017.01424

subject

Has Abstract

pub_date

2017-08-02 00:00:00

pages

1424

issn

1664-302X

journal_volume

8

pub_type

杂志文章
  • The Gut Microbiome as a Major Regulator of the Gut-Skin Axis.

    abstract::The adult intestine hosts a myriad of diverse bacterial species that reside mostly in the lower gut maintaining a symbiosis with the human habitat. In the current review, we describe the neoteric advancement in our comprehension of how the gut microbiota communicates with the skin as one of the main regulators in the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.01459

    authors: Salem I,Ramser A,Isham N,Ghannoum MA

    更新日期:2018-07-10 00:00:00

  • Expanding genomics of mycorrhizal symbiosis.

    abstract::The mycorrhizal symbiosis between soil fungi and plant roots is a ubiquitous mutualism that plays key roles in plant nutrition, soil health, and carbon cycling. The symbiosis evolved repeatedly and independently as multiple morphotypes [e.g., arbuscular mycorrhizae (AM), ectomycorrhizal (ECM)] in multiple fungal clade...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00582

    authors: Kuo A,Kohler A,Martin FM,Grigoriev IV

    更新日期:2014-11-04 00:00:00

  • Isolation, Identification, and Complete Genome Assembly of an Endophytic Bacillus velezensis YB-130, Potential Biocontrol Agent Against Fusarium graminearum.

    abstract::Wheat scab caused by F. graminearum is a highly destructive disease that leads to yield reduction and mycotoxin contamination of grains. In this study, an endophytic bacterium of strain YB-130 was isolated from surface sterilized wheat spikes with scab symptoms and identified as Bacillus velezensis by whole genome ann...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.598285

    authors: Xu W,Zhang L,Goodwin PH,Xia M,Zhang J,Wang Q,Liang J,Sun R,Wu C,Yang L

    更新日期:2020-12-03 00:00:00

  • Genomic Features for Desiccation Tolerance and Sugar Biosynthesis in the Extremophile Gloeocapsopsis sp. UTEX B3054.

    abstract::For tolerating extreme desiccation, cyanobacteria are known to produce both compatible solutes at intracellular level and a copious amount of exopolysaccharides as a protective coat. However, these molecules make cyanobacterial cells refractory to a broad spectrum of cell disruption methods, hindering genome sequencin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00950

    authors: Urrejola C,Alcorta J,Salas L,Vásquez M,Polz MF,Vicuña R,Díez B

    更新日期:2019-05-07 00:00:00

  • Semi-Quantification of Total Campylobacter and Salmonella During Egg Incubations Using a Combination of 16S rDNA and Specific Pathogen Primers for qPCR.

    abstract::Rapid molecular techniques that evaluate eggs for the presence of foodborne pathogens is an essential component to poultry food safety monitoring. Interestingly, it is not just table eggs that contribute to outbreaks of foodborne disease. Broiler layer production actively contributes to sustaining of foodborne pathoge...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02454

    authors: Rothrock MJ Jr,Feye KM,Kim SA,Park SH,Locatelli A,Hiett KL,Gamble J,Sellers H,Ricke SC

    更新日期:2018-11-02 00:00:00

  • Strong Inference in Mathematical Modeling: A Method for Robust Science in the Twenty-First Century.

    abstract::While there are many opinions on what mathematical modeling in biology is, in essence, modeling is a mathematical tool, like a microscope, which allows consequences to logically follow from a set of assumptions. Only when this tool is applied appropriately, as microscope is used to look at small items, it may allow to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01131

    authors: Ganusov VV

    更新日期:2016-07-22 00:00:00

  • Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein From Halobacillus andaensis and Functional Analysis of Its Conserved Arginine/Aspartate Residues.

    abstract::Arginine-aspartate-aspartate (RDD) family, representing a category of transmembrane proteins containing one highly conserved arginine and two highly conserved aspartates, has been functionally uncharacterized as yet. Here we present the characterization of a member of this family designated RDD from the moderate halop...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00807

    authors: Shao L,Abdel-Motaal H,Chen J,Chen H,Xu T,Meng L,Zhang Z,Meng F,Jiang J

    更新日期:2018-04-25 00:00:00

  • Same, same but different: symbiotic bacterial associations in GBR sponges.

    abstract::Symbioses in marine sponges involve diverse consortia of microorganisms that contribute to the health and ecology of their hosts. The microbial communities of 13 taxonomically diverse Great Barrier Reef (GBR) sponge species were assessed by DGGE and 16S rRNA gene sequencing to determine intra and inter species variati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00444

    authors: Webster NS,Luter HM,Soo RM,Botté ES,Simister RL,Abdo D,Whalan S

    更新日期:2013-01-18 00:00:00

  • Source Tracking Based on Core Genome SNV and CRISPR Typing of Salmonella enterica Serovar Heidelberg Isolates Involved in Foodborne Outbreaks in Québec, 2012.

    abstract::Whole-genome sequencing (WGS) is the method of choice for bacterial subtyping and it is rapidly replacing the more traditional methods such as pulsed-field gel electrophoresis (PFGE). Here we used the high-resolution core genome single nucleotide variant (cgSNV) typing method to characterize clinical and food from Sal...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01317

    authors: Yousfi K,Usongo V,Berry C,Khan RH,Tremblay DM,Moineau S,Mulvey MR,Doualla-Bell F,Fournier E,Nadon C,Goodridge L,Bekal S

    更新日期:2020-06-17 00:00:00

  • A Pilin Region Affecting Host Range of the Pseudomonas aeruginosa RNA Phage, PP7.

    abstract::The host range of a phage is determined primarily by phage-receptor interaction. Here, we profiled the host range of an RNA leviphage, PP7 that requires functional type IV pilus (TFP) in order to enter into its host bacterium, Pseudomonas aeruginosa. Out of 25 twitching-proficient P. aeruginosa strains, 4 with group I...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00247

    authors: Kim ES,Bae HW,Cho YH

    更新日期:2018-02-16 00:00:00

  • Negative Plant-Soil Feedback Driven by Re-assemblage of the Rhizosphere Microbiome With the Growth of Panax notoginseng.

    abstract::There is a concerted understanding of the accumulation of soil pathogens as the major driving factor of negative plant-soil feedback (NPSF). However, our knowledge of the connection between plant growth, pathogen build-up and soil microbiome assemblage is limited. In this study, significant negative feedback between t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01597

    authors: Luo L,Guo C,Wang L,Zhang J,Deng L,Luo K,Huang H,Liu Y,Mei X,Zhu S,Yang M

    更新日期:2019-07-26 00:00:00

  • Involvement of a Membrane-Bound Amphiphilic Helix in Substrate Discrimination and Binding by an Escherichia coli S2P Peptidase RseP.

    abstract::Intramembrane proteases (IMPs) are a unique class of proteases that catalyze the proteolysis within the membrane and regulate diverse cellular processes in various organisms. RseP, an Escherichia coli site-2 protease (S2P) family IMP, is involved in the regulation of an extracytoplasmic stress response through the cle...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.607381

    authors: Miyake T,Hizukuri Y,Akiyama Y

    更新日期:2020-11-27 00:00:00

  • Nutrient Composition Promotes Switching between Pellicle and Bottom Biofilm in Salmonella.

    abstract::Salmonella is one of the most frequently reported causes of foodborne illness worldwide. Non-typhoidal serovars cause gastroenteritis in humans. Salmonella can grow on surfaces forming biofilms, contributing to its persistence since biofilms are difficult to eradicate due to the high resistance to antimicrobials and d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02160

    authors: Paytubi S,Cansado C,Madrid C,Balsalobre C

    更新日期:2017-11-07 00:00:00

  • Population Kinetics and Mechanistic Aspects of Saccharomyces cerevisiae Growth in Relation to Selenium Sulfide Nanoparticle Synthesis.

    abstract::Biosynthesis of nanoparticles (NPs) by microorganisms is a cost- and energy-effective approach. However, how the production of NPs affects the population of producing organism remains as an unresolved question. The present study aimed to evaluate the kinetics of Saccharomyces cerevisiae growth in relation to synthesis...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01019

    authors: Asghari-Paskiabi F,Imani M,Eybpoosh S,Rafii-Tabar H,Razzaghi-Abyaneh M

    更新日期:2020-05-21 00:00:00

  • Excretion of antibiotic resistance genes by dairy calves fed milk replacers with varying doses of antibiotics.

    abstract::Elevated levels of antibiotic resistance genes (ARGs) in soil and water have been linked to livestock farms and in some cases feed antibiotics may select for antibiotic resistant gut microbiota. The purpose of this study was to examine the establishment of ARGs in the feces of calves receiving milk replacer containing...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00139

    authors: Thames CH,Pruden A,James RE,Ray PP,Knowlton KF

    更新日期:2012-04-10 00:00:00

  • Exploring the Genome and Phenotype of Multi-Drug Resistant Klebsiella pneumoniae of Clinical Origin.

    abstract::Klebsiella pneumoniae is an important nosocomial pathogen with an extraordinary resistant phenotype due to a combination of acquired resistant-elements and efflux mechanisms. In this study a detailed molecular characterization of 11 K. pneumoniae isolates of clinical origin was carried out. Eleven clinical isolates we...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01913

    authors: Anes J,Hurley D,Martins M,Fanning S

    更新日期:2017-10-23 00:00:00

  • The Anti-mycobacterial Activity of a Diterpenoid-Like Molecule Operates Through Nitrogen and Amino Acid Starvation.

    abstract::A library of 14 minimally cytotoxic diterpenoid-like compounds (CC50 > 70 μM on HepG2 human liver cells) was screened against Mycobacterium smegmatis, Staphylococcus aureus, and Escherichia coli to determine antimicrobial activity. Some compounds with a phenethyl alcohol (PE) core substituted with a β-cyclocitral deri...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01444

    authors: Crusco A,Baptista R,Bhowmick S,Beckmann M,Mur LAJ,Westwell AD,Hoffmann KF

    更新日期:2019-06-25 00:00:00

  • Stuck in the Middle: Fibronectin-Binding Proteins in Gram-Positive Bacteria.

    abstract::Fibronectin is a multidomain glycoprotein found ubiquitously in human body fluids and extracellular matrices of a variety of cell types from all human tissues and organs, including intestinal epithelial cells. Fibronectin plays a major role in the regulation of cell migration, tissue repair, and cell adhesion. Importa...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01504

    authors: Hymes JP,Klaenhammer TR

    更新日期:2016-09-22 00:00:00

  • Efficacy of Live-Attenuated H9N2 Influenza Vaccine Candidates Containing NS1 Truncations against H9N2 Avian Influenza Viruses.

    abstract::H9N2 avian influenza virus is a zoonotic agent with a broad host range that can contribute genetic information to H5 or H7N9 subtype viruses, which are significant threats to both humans and birds. Thus, there is a great need for a vaccine to control H9N2 avian influenza. Three mutant viruses of an H9N2 virus A/chicke...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01086

    authors: Chen S,Zhu Y,Yang D,Yang Y,Shi S,Qin T,Peng D,Liu X

    更新日期:2017-06-14 00:00:00

  • Genomic Reconstruction of Carbohydrate Utilization Capacities in Microbial-Mat Derived Consortia.

    abstract::Two nearly identical unicyanobacterial consortia (UCC) were previously isolated from benthic microbial mats that occur in a heliothermal saline lake in northern Washington State. Carbohydrates are a primary source of carbon and energy for most heterotrophic bacteria. Since CO2 is the only carbon source provided, the c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01304

    authors: Leyn SA,Maezato Y,Romine MF,Rodionov DA

    更新日期:2017-07-13 00:00:00

  • Biocontrol Potentials of Antimicrobial Peptide Producing Bacillus Species: Multifaceted Antagonists for the Management of Stem Rot of Carnation Caused by Sclerotinia sclerotiorum.

    abstract::Bacillus species are widely exploited as biocontrol agents because of their efficiency in impeding various plant pathogens with multifaceted approach. In this study, Bacillus species were isolated from rhizosphere of various plants viz., carnations, cotton, turmeric, and bananas in Tamil Nadu state of India. Their pot...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00446

    authors: Vinodkumar S,Nakkeeran S,Renukadevi P,Malathi VG

    更新日期:2017-03-24 00:00:00

  • Comparative Functional Genomic Analysis of Two Vibrio Phages Reveals Complex Metabolic Interactions with the Host Cell.

    abstract::Sequencing and annotation was performed for two large double stranded DNA bacteriophages, φGrn1 and φSt2 of the Myoviridae family, considered to be of great interest for phage therapy against Vibrios in aquaculture live feeds. In addition, phage-host metabolic interactions and exploitation was studied by transcript pr...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01807

    authors: Skliros D,Kalatzis PG,Katharios P,Flemetakis E

    更新日期:2016-11-14 00:00:00

  • Focused Review: Cytotoxic and Antioxidant Potentials of Mangrove-Derived Streptomyces.

    abstract::Human life expectancy is rapidly increasing with an associated increasing burden of chronic diseases, such as neurodegenerative diseases and cancer. However, there is limited progress in finding effective treatment for these conditions. For this reason, members of the genus Streptomyces have been explored extensively ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2017.02065

    authors: Ser HL,Tan LT,Law JW,Chan KG,Duangjai A,Saokaew S,Pusparajah P,Ab Mutalib NS,Khan TM,Goh BH,Lee LH

    更新日期:2017-11-01 00:00:00

  • Natural Killer Cell Functions during the Innate Immune Response to Pathogenic Streptococci.

    abstract::Dendritic cells (DCs) and NK cells play a crucial role in the first phase of host defense against infections. Group B Streptococcus (GBS) and Streptococcus suis are encapsulated streptococci causing severe systemic inflammation, leading to septicemia and meningitis. Yet, the involvement of NK cells in the innate immun...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01196

    authors: Lemire P,Galbas T,Thibodeau J,Segura M

    更新日期:2017-06-29 00:00:00

  • Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry-Based Metabolomics Reveals Metabolic Alterations in the Mouse Cerebellum During Toxoplasma gondii Infection.

    abstract::Toxoplasma gondii is a protozoan parasite with a remarkable neurotropism. We recently showed that T. gondii infection can alter the global metabolism of the cerebral cortex of mice. However, the impact of T. gondii infection on the metabolism of the cerebellum remains unknown. Here we apply metabolomic profiling to di...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01555

    authors: Ma J,He JJ,Hou JL,Zhou CX,Elsheikha HM,Zhu XQ

    更新日期:2020-07-10 00:00:00

  • The Potential Role of Arbuscular Mycorrhizal Fungi in the Restoration of Degraded Lands.

    abstract::Experiences worldwide reveal that degraded lands restoration projects achieve little success or fail. Hence, understanding the underlying causes and accordingly, devising appropriate restoration mechanisms is crucial. In doing so, the ever-increasing aspiration and global commitments in degraded lands restoration coul...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01095

    authors: Asmelash F,Bekele T,Birhane E

    更新日期:2016-07-26 00:00:00

  • Simultaneous Discovery of Positive and Negative Interactions Among Rhizosphere Bacteria Using Microwell Recovery Arrays.

    abstract::Understanding microbe-microbe interactions is critical to predict microbiome function and to construct communities for desired outcomes. Investigation of these interactions poses a significant challenge due to the lack of suitable experimental tools available. Here we present the microwell recovery array (MRA), a new ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.601788

    authors: Barua N,Herken AM,Stern KR,Reese S,Powers RL,Morrell-Falvey JL,Platt TG,Hansen RR

    更新日期:2021-01-05 00:00:00

  • Genomics of Serratia marcescens Isolates Causing Outbreaks in the Same Pediatric Unit 47 Years Apart: Position in an Updated Phylogeny of the Species.

    abstract::The first documented nosocomial outbreak caused by Serratia marcescens in Spain occurred in 1969 at the neonatal intensive care unit (NICU) of the tertiary La Paz Children's Hospital in Madrid, Spain, and based on the available phenotyping techniques at this time, it was considered as a monoclonal outbreak. Only 47 ye...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00451

    authors: Saralegui C,Ponce-Alonso M,Pérez-Viso B,Moles Alegre L,Escribano E,Lázaro-Perona F,Lanza VF,de Pipaón MS,Rodríguez JM,Baquero F,Del Campo R

    更新日期:2020-03-31 00:00:00

  • Machine Learning Approaches for Epidemiological Investigations of Food-Borne Disease Outbreaks.

    abstract::Foodborne diseases (FBDs) are infections of the gastrointestinal tract caused by foodborne pathogens (FBPs) such as bacteria [Salmonella, Listeria monocytogenes and Shiga toxin-producing E. coli (STEC)] and several viruses, but also parasites and some fungi. Artificial intelligence (AI) and its sub-discipline machine ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.01722

    authors: Vilne B,Meistere I,Grantiņa-Ieviņa L,Ķibilds J

    更新日期:2019-08-06 00:00:00

  • Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk.

    abstract::Nisin, an important bacteriocin from Lactococcus lactis subsp., is primarily active against various Gram-positive bacteria. Leucocin C, produced by Leuconostoc carnosum 4010, is a class IIa bacteriocin used to inhibit the growth of Listeria monocytogenes. Because two bacteriocins have different modes of action, the co...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00547

    authors: Fu Y,Mu D,Qiao W,Zhu D,Wang X,Liu F,Xu H,Saris P,Kuipers OP,Qiao M

    更新日期:2018-03-23 00:00:00