Data management and data enrichment for systems biology projects.

abstract：:Yarrowia lipolytica can accumulate large amounts of storage lipids and has considerable potential for the production of polyunsaturated fatty acids and other lipids for biofuels. When the nitrogen source is exhausted in the medium, the key intermediate, citrate, is converted to acetyl-CoA by ATP:citrate lyase (ACL) fo...

journal_title：Journal of biotechnology

authors： Zhang H,Zhang L,Chen H,Chen YQ,Chen W,Song Y,Ratledge C

更新日期：2014-12-20 00:00:00

abstract：:The xylanase gene from Gloeophyllum trabeum was cloned and expressed in Pichia pastoris GS115. Xyl10g has a molecular weight of approximately 50kDa, and exhibits maximum specific activity at 70°C and a broad range of pH 4.0-7.0. Purified recombinant Xyl10g efficiently degraded popping-pretreated corn stover and newspa...

journal_title：Journal of biotechnology

authors： Kim HM,Lee KH,Kim KH,Lee DS,Nguyen QA,Bae HJ

更新日期：2014-02-20 00:00:00

abstract：:Based on the prototype experiment of treating herb wastewater by Up flow Anaerobic Sludge Bed and Anaerobic Filter reactor (UASBAF), an artificial neural network (ANN) model which adopts a back propagation algorithm with momentum and adaptive learning rate was established. And the effect of each parameter to the perfo...

journal_title：Journal of biotechnology

authors： Shi Y,Zhao XT,Zhang YM,Ren NQ

更新日期：2009-10-12 00:00:00

abstract：:Functional screening for lipolytic enzymes from a metagenomic library (origin: Jae Sawn hot spring, Thailand) resulted in isolation of a novel patatin-like phospholipase (PLP) and an esterase (Est1). PLP contained four conserved domains similar to other patatin-like proteins with lipid acyl hydrolase activity. Likewis...

journal_title：Journal of biotechnology

authors： Tirawongsaroj P,Sriprang R,Harnpicharnchai P,Thongaram T,Champreda V,Tanapongpipat S,Pootanakit K,Eurwilaichitr L

更新日期：2008-01-01 00:00:00

abstract：:The PRNP gene encodes a cellular protein named prion, whose misfolded form has been implicated in a number of neuropathic diseases in mammals such as the Bovine Spongiform Encephalopathy (BSE) in cattle. BSE has brought devastating impact on the world economy and human health. Recently, several groups have performed t...

journal_title：Journal of biotechnology

authors： Wang S,Zhang K,Ding F,Zhao R,Li S,Li R,Xu L,Song C,Dai Y,Li N

更新日期：2013-02-20 00:00:00

abstract：:The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine ...

journal_title：Journal of biotechnology

authors： Capiaumont J,Legrand C,Carbonell D,Dousset B,Belleville F,Nabet P

更新日期：1995-02-21 00:00:00

abstract：:Indigo is an insoluble blue dye, which generates serious pollution in its production process. Increasing focus has come to the biosynthesis of indigo that are more environment-preserved and high-efficient. Hence, this study was designed to explore the specific role of various deep eutectic solvents (DESs) on cytochrom...

journal_title：Journal of biotechnology

authors： Sun H,Xin R,Qu D,Yao F

更新日期：2020-11-10 00:00:00

abstract：:Recombinant human granulocyte colony stimulating factor (rhG-CSF) and its PEGylated product "mono-PEG20-GCSF" have already been widely used for treatment of all kinds of neutropenia. However, the high required dosage of mono-PEG20-GCSF made it relatively expensive in clinical use. We postulated that an N-terminal site...

journal_title：Journal of biotechnology

authors： Zhai Y,Zhao Y,Lei J,Su Z,Ma G

更新日期：2009-07-15 00:00:00

abstract：:siRNA based therapeutics have become the next frontier in molecular medicine. Though exosomes emerge as a promising drug delivery vehicle for siRNAs, significant hurdle remains in finding safe and effective loading methods. Traditional methods of loading exogenous siRNAs in exosomes are marked by certain limitations l...

journal_title：Journal of biotechnology

authors： Shandilya S,Rani P,Onteru SK,Singh D

更新日期：2020-07-20 00:00:00

abstract：:A critical step in the development of robust Agrobacterium tumefaciens-mediated transformation system in recalcitrant grain legume, cowpea is the establishment of optimal conditions for efficient T-DNA delivery into target tissue and recovery of transgenic plants. A dramatic increase in efficiency of T-DNA delivery wa...

journal_title：Journal of biotechnology

authors： Solleti SK,Bakshi S,Sahoo L

更新日期：2008-05-20 00:00:00

abstract：:Rebuilding enzymes with high activities, stability and reusability has been constantly pursued for application of enzymaticcatalysis in wide field. Rebuilding enzymes by chemical approaches, including immobilization and chemicalmodification, represents a simple but effective route. Here, a class of highly stable and a...

journal_title：Journal of biotechnology

authors： Li L,Li H,Yan B,Yu S

更新日期：2019-02-10 00:00:00

abstract：:Insertion of foreign DNA into plant genomes occurs randomly and with low frequency. Hence, a selectable marker is generally required to identify transgenic plants. Until now, all selection systems have been based on the use of non-plant genes, derived from microorganisms and usually conferring antibiotic or herbicide ...

journal_title：Journal of biotechnology

authors： Leyman B,Avonce N,Ramon M,Van Dijck P,Iturriaga G,Thevelein JM

更新日期：2006-02-10 00:00:00

abstract：:In this paper we report the development of a recombinant strain of the yeast Pichia pastoris, which secretes an anti-carcinoembryonic antigen single chain Fv (scFv) antibody fragment to the culture supernatant as a biologically active protein, at levels of 1.2 g l(-1). The yeast scFv was purified by IMAC, with a final...

journal_title：Journal of biotechnology

authors： Freyre FM,Vázquez JE,Ayala M,Canaán-Haden L,Bell H,Rodríguez I,González A,Cintado A,Gavilondo JV

更新日期：2000-01-21 00:00:00

abstract：:A stably transformed insect cell expression system is superior to a baculovirus expression system, since the expression system is sustained and there is no cell lysis, but the isolation of cell lines producing recombinant proteins is time-consuming and laborious. In this study, we developed a technique for the rapid i...

journal_title：Journal of biotechnology

authors： Kato T,Yoshizuka K,Park EY

更新日期：2010-05-17 00:00:00

abstract：:Different systems are being studied and used to prepare recombinant proteins for pharmaceutical use. The blood, and still more the milk, from transgenic animals appear a very attractive source of pharmaceuticals. The cells from these animals are expected to produce well-matured proteins in potentially huge amounts. Se...

journal_title：Journal of biotechnology

authors： Houdebine LM

更新日期：1994-05-31 00:00:00

abstract：:Acetogenic bacteria like Acetobacterium woodii represent an ancient group of anaerobic microorganisms which use hydrogen and carbon dioxide to produce acetate. Cell concentrations and space-time yields are usually low in gas fermentations. A standard stirred‑tank bioreactor with continuous gas supply was equipped with...

journal_title：Journal of biotechnology

authors： Kantzow C,Mayer A,Weuster-Botz D

更新日期：2015-10-20 00:00:00

abstract：:A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) is a novel family of extracellular proteases supposedly involved in inflammation, angiogenesis, development and coagulation. To overexpress the active ADAMTS proteins, we designed a chimeric molecule composed of a catalytic domain of ADAMTS-1...

journal_title：Journal of biotechnology

authors： Sugiura T

更新日期：2003-02-13 00:00:00

abstract：:4-vinylguaiacol, a kind of volatile phenolic compound with tobacco flavor, is widely used as a component of edible flavor and intermediate of medicine. Ferulic acid is usually used as substrate for the biosynthesis of 4-vinylguaiacol. However, the price of ferulic acid is high, leading to high production cost. In this...

journal_title：Journal of biotechnology

authors： Sun LH,Lv SW,Yu F,Li SN,He LY

更新日期：2018-09-10 00:00:00

abstract：:The first complete genome sequence of Bacillus glycinifermentans B-27 was determined by SMRT sequencing generating a genome sequence with a total length of 4,607,442 bases. Based on this sequence 4738 protein-coding sequences were predicted and used to identify gene clusters that are related to the production of secon...

journal_title：Journal of biotechnology

authors： Stadermann KB,Blom J,Borgmeier C,Sciberras N,Herbold S,Kipker M,Meurer G,Molck S,Petri D,Pelzer S,Schneider J

更新日期：2017-09-10 00:00:00

abstract：:AL (SpA A domain-PpL B3 domain), LD5 (PpL B3 domain-SpA D domain-PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L-D-L) and LD3 (PpL B3 domain-SpA D domain-PpL B3 domain, L-D-L) are novel evolved Ig binding molecules (NEIBMs) derived from the in vitro molecular evolution of combinatorial phage libraries displaying rando...

journal_title：Journal of biotechnology

authors： He T,Ding YY,Feng JJ,Chen QL,Zhu HM,Peng H,Rui B,Li XY,Cao MM,Pan W

更新日期：2014-08-20 00:00:00

abstract：:Extracts of the medicinal plant Hypericum perforatum are used to treat depression and skin irritation. A major API is hyperforin, characterized by sensitivity to light, oxygen and temperature. Total synthesis of hyperforin is challenging and its content in field-grown plants is variable. We have established in vitro c...

journal_title：Journal of biotechnology

authors： Gaid M,Haas P,Beuerle T,Scholl S,Beerhues L

更新日期：2016-03-20 00:00:00

abstract：:The genus Enterococcus occurs as native microbiota of fermented products due to its broad environmental distribution and its resistance to salt concentrations. Enterococcus faecalis F, a non-pathogenic strain isolated from a ripened cheese, has demonstrated useful enzymatic capabilities, a probiotic behavior and antib...

journal_title：Journal of biotechnology

authors： Serrano-Maldonado CE,Quirasco M

更新日期：2018-10-10 00:00:00

abstract：:Phycocyanin (PC) prepared from a cyanobacterium Spirulina platensis by the DEAE-DE52 cellulose column chromatography that was developed by gradient elution of 50-250 mM phosphate buffer (pH 7.0) was stabilized by its subunits cross-linked covalently with formaldehyde. The single blue band that the chemically stabilize...

journal_title：Journal of biotechnology

authors： Sun L,Wang S,Qiao Z

更新日期：2006-02-24 00:00:00

abstract：:Many recent epigenetic studies utilize the advantages of CRISPR/dCas9 based tools in linking certain epigenetic modification with gene expression regulation. Various multifactorial diseases often contain changed epigenetic signatures at many loci, so tools for simultaneously targeting different loci would significantl...

journal_title：Journal of biotechnology

authors： Josipović G,Zoldoš V,Vojta A

更新日期：2019-08-10 00:00:00

abstract：:Aptamers are single-stranded DNA or RNA oligonucleotides, which have been emerging as recognition elements in disease diagnostics and food control, including the detection of bacterial toxins. In this study, we employed the semi-automated just in time-selection to identify aptamers that bind to cholera toxin (CT) with...

journal_title：Journal of biotechnology

authors： Frohnmeyer E,Frisch F,Falke S,Betzel C,Fischer M

更新日期：2018-03-10 00:00:00

abstract：:Escherichia coli overexpressing a NAD(+)-dependent formate dehydrogenase (FDH) from Candida boidinii was grown in chemostat culture on various carbon sources at 0.05 h(-1) dilution rate, under anaerobic conditions using defined medium and compared to a control without the heterologous FDH pathway. Metabolic fluxes, NA...

journal_title：Journal of biotechnology

authors： Sánchez AM,Bennett GN,San KY

更新日期：2005-06-29 00:00:00

abstract：:It has been well recognized that many key aspects of cell cycle regulation are encoded into the size distributions of growing budding yeast populations due to the tight coupling between cell growth and cell division present in this organism. Several attempts have been made to model the cell size distribution of growin...

journal_title：Journal of biotechnology

authors： Hatzis C,Porro D

更新日期：2006-07-13 00:00:00

abstract：:With the objective of monitoring xenobiotic degrading bacteria in soil, a method for rapid extraction of DNA from soil, amenable to amplification by PCR, was developed. The method was based on lysis by freeze-thawing and subsequent addition of sodium dodecyl sulfate (SDS), hexadecyltrimethylammonium bromide and protei...

journal_title：Journal of biotechnology

authors： Wikström P,Wiklund A,Andersson AC,Forsman M

更新日期：1996-12-10 00:00:00

abstract：:Paenibacillus riograndensis is a Gram-positive rhizobacterium which exhibits plant growth promoting activities. It was isolated from the rhizosphere of wheat grown in the state of Rio Grande do Sul, Brazil. Here we announce the complete genome sequence of P. riograndensis strain SBR5(T). The genome of P. riograndensis...

journal_title：Journal of biotechnology

authors： Brito LF,Bach E,Kalinowski J,Rückert C,Wibberg D,Passaglia LM,Wendisch VF

更新日期：2015-08-10 00:00:00

abstract：:Enzymatic hydrolysis constitutes an attractive strategy for biorefining of abundant, low-cost agricultural by-products such as wheat bran and straw. However, to adopt such an approach, efficient enzymes are required, in particular xylanases. To promote heat-induced disorganization of the complex cell wall network in w...

journal_title：Journal of biotechnology

authors： Paës G,O'Donohue MJ

更新日期：2006-09-18 00:00:00