当前位置： SCI文献检索 > ACTA PHARMACOLOGICA SINICA期刊下所有文献 > Chinese medicine CGA formula ameliorates DMN-induced liver fibrosis in rats via inhibiting MMP2/9, TIMP1/2 and the TGF-β/Smad signaling pathways.

Chinese medicine CGA formula ameliorates DMN-induced liver fibrosis in rats via inhibiting MMP2/9, TIMP1/2 and the TGF-β/Smad signaling pathways.

Abstract：

AIM:Chinese medicine CGA formula consists of polysaccharide from Cordyceps sinensis mycelia (CS-PS), gypenosides and amygdalin, which is derived from Fuzheng Huayu (FZHY) capsule for treating liver fibrosis. In this study we attempted to confirm the therapeutic effects of CGA formula in dimethylnitrosamine (DMN)-induced liver fibrosis in rats, and to identify the mechanisms of anti-fibrotic actions. METHODS:Rats were injected with DMN (10 mg·kg(-1)·d(-1), ip) for 3 consecutive days per week over a 4-week period. The rats then were orally administered with CGA formula (CS-PS 60 mg·kg(-1)·d(-1), gypenosides 50 mg·kg(-1)·d(-1) and amygdalin 80 mg·kg(-1)·d(-1)) daily in the next 2 weeks. CS-PS, gypenosides or amygdalin alone were administered as individual component controls, whereas colchicine and FZHY were used as positive controls. Serum biomarkers were measured. Hepatic injury, collagen deposition and stellate cell activation were examined. The MMP activities, expression of TIMP protein and proteins involved in the TGF-β1/Smad signaling pathways in liver tissues were assayed. RESULTS:In DMN-treated rats, administration of CGA formula significantly decreased serum ALT, AST and total bilirubin and hepatic hydroxyproline levels, increased serum albumin level, and attenuated liver fibrosis as shown by histological examination. Furthermore, these effects were comparable to those caused by administration of FZHY, and superior to those caused by administration of colchicine or the individual components of CGA formula. Moreover, administration of CGA formula significantly decreased the protein levels of α-SMA, TGF-β1, TGF-β1 receptor (TβR-I), p-TβR-I, p-TβR-II, p-Smad2, p-Smad3, TIMP1 and TIMP2, as well as MMP2 and MMP9 activities in liver tissues of DMN-treated rats. CONCLUSION:Chinese medicine CGA formula ameliorates DMN-induced liver fibrosis in rats, and this effect was likely associated with the down-regulation of MMP2/9 activities, TIMP1/2 protein expression and the TGF-β1/Smad signaling pathways in the liver.

journal_name

Acta Pharmacol Sin

journal_title

Acta pharmacologica Sinica

authors

Li XM,Peng JH,Sun ZL,Tian HJ,Duan XH,Liu L,Ma X,Feng Q,Liu P,Hu YY

doi

10.1038/aps.2016.35

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

783-93

issue

6

eissn

1671-4083

issn

1745-7254

pii

aps201635

journal_volume

37

pub_type

杂志文章

相关文献

ACTA PHARMACOLOGICA SINICA文献大全
  • Simvastatin suppresses vascular inflammation and atherosclerosis in ApoE(-/-) mice by downregulating the HMGB1-RAGE axis.

    abstract：AIM:High mobility group box protein 1 (HMGB1) and receptor for the advanced glycation end product (RAGE) play pivotal roles in vascular inflammation and atherosclerosis. The aim of this study was to determine whether the HMGB1-RAGE axis was involved in the actions of simvastatin on vascular inflammation and atheroscler...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2013.8

    authors： Liu M,Yu Y,Jiang H,Zhang L,Zhang PP,Yu P,Jia JG,Chen RZ,Zou YZ,Ge JB

    更新日期：2013-06-01 00:00:00

  • Localized Ca2+ uncaging induces Ca2+ release through IP3R in smooth muscle.

    abstract：AIM:Our previous study indicated that there are two types of Ca2+ release events seen in intact mouse bladder tissue. In this study our aim is to investigate the mechanism that underlies the phenomena of Ca2+ release in smooth muscle. METHODS:Single cells were isolated and tissue segments were prepared by cutting the ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2006.00389.x

    authors： Wang M,Chen Z,Xing Y,Zhang X,Dong XZ,Ji GJ

    更新日期：2006-07-01 00:00:00

  • NK3 and NK4 of HGF enhance filamin production via STAT pathway, but not NK1 and NK2 in human breast cancer cells.

    abstract：AIM:The purpose of this study was to reveal the effects of hepatocyte growth factor (HGF) variants on human breast cancer cells and the differential signaling pathways of the variants in controlling cell proliferation and invasion. METHODS:Four HGF variants (NK1, NK2, NK3, and NK4) were created by gene engineering, an...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2008.00799.x

    authors： Yin YL,Chen HL,Kuo HM,He SP

    更新日期：2008-06-01 00:00:00

  • Recombinant adeno-associated virus-mediated human kallikrein gene therapy protects against hypertensive target organ injuries through inhibiting cell apoptosis.

    abstract：AIM:Overexpression of human tissue kallikrein (HK), mediated by recombinant adeno-associated virus (rAAV), decreased blood pressure in spontaneous hypertensive rats (SHRs) and reduced injury to the heart, aorta and kidney. In this study, we used both an in vivo animal model and in vitro cell culture system to investiga...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2009.114

    authors： Yan JT,Wang T,Wang DW

    更新日期：2009-09-01 00:00:00

  • Hydrogen sulfide: third gaseous transmitter, but with great pharmacological potential.

    abstract：:Hydrogen sulfide (H2S), which is well known traditionally as a toxic gas, has been proven to be produced endogenously by 3 enzymes in mammalian tissues and plays important roles in physiological and pathophysiological conditions. In the central nervous system, H2S functions as not only a neuromodulator, but also a neu...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1111/j.1745-7254.2007.00629.x

    authors： Chen CQ,Xin H,Zhu YZ

    更新日期：2007-11-01 00:00:00

  • Pharmacokinetic characteristics of L-valyl-ara-C and its implication on the oral delivery of ara-C.

    abstract：AIM:To evaluate the pharmacokinetic characteristics of L-valyl-ara-C, a peptidomimetic prodrug of ara-C. METHODS:After the synthesis of L-valyl-ara-C, the in vitro stability of L-valyl-ara-C was examined in various biological media. Plasma pharmacokinetic profiles of ara-C and L-valyl-ara-C were also evaluated in rats...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2007.00474.x

    authors： Cheon EP,Han HK

    更新日期：2007-02-01 00:00:00

  • The novel squamosamide derivative FLZ enhances BDNF/TrkB/CREB signaling and inhibits neuronal apoptosis in APP/PS1 mice.

    abstract：AIM:The aim of this study was to study the effects of compound FLZ, a novel cyclic derivative of squamosamide from Annona glabra, on brain-derived neurotrophic factor (BDNF)/tropomyosin receptor kinase B (TrkB)/cAMP response element-binding protein (CREB) signaling and neuronal apoptosis in the hippocampus of the amylo...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2010.3

    authors： Li N,Liu GT

    更新日期：2010-03-01 00:00:00

  • Cycloastragenol upregulates SIRT1 expression, attenuates apoptosis and suppresses neuroinflammation after brain ischemia.

    abstract：:Cycloastragenol (CAG) is the active form of astragaloside IV isolated from Astragalus Radix, which displays multiple pharmacological effects. Silent information regulator 1 (SIRT1), a class III histone deacetylase, has been shown to play an important role in neuroprotection against cerebral ischemia. In this study, we...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/s41401-020-0386-6

    authors： Li M,Li SC,Dou BK,Zou YX,Han HZ,Liu DX,Ke ZJ,Wang ZF

    更新日期：2020-08-01 00:00:00

  • Affinity of anticancer drug, daunomycin, to core histones in solution: comparison of free and cross-linked proteins.

    abstract：AIM:The interaction of anthracycline anticancer drugs with chromatin, nucleosomes and histone H1 has been extensively studied. In the present study, for the first time, we have investigated the binding of anthracycline antibiotic, daunomycin, to free and cross-linked thymus core histones (CL-core) in solution and in th...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2007.00542.x

    authors： Rabbani A,Abdosamadi S,Sari-Saraf N

    更新日期：2007-05-01 00:00:00

  • Effect of theophylline on airway inflammation in asthma.

    abstract：AIM:To investigate whether low dose theophylline has an anti-inflammatory effect in asthma. METHODS:Nineteen asthmatic subjects were given 200 mg sustained-release theophylline preparation twice daily for 4 weeks. The mean serum concentration of theophylline was 7.9 mg/L. The percentage of eosinophils, EG2+ eosinophil...

    journal_title：Acta pharmacologica Sinica

    pub_type： 临床试验,杂志文章

    doi：

    authors： Yang J,Tu HY,Li QQ

    更新日期：2001-05-01 00:00:00

  • M2 muscarinic receptor of spinal cord mediated increase of nNOS expression in locus coeruleus during morphine withdrawal.

    abstract：AIM:To investigate the effects of different muscarinic receptor (M) subtypes in the spinal cord on the scores of naloxone-precipitated morphine-withdrawal symptoms and the changes of nNOS expression in locus coeruleus (LC). METHODS:nNOS immunohistochemistry, intrathecal injection (it), and antisense oligonucleotides (...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Chou WB,Zeng YM,Duan SM,Zhou WH,Gu J,Yang GD

    更新日期：2002-08-01 00:00:00

  • Evaluation of doxorubicin-loaded pH-sensitive polymeric micelle release from tumor blood vessels and anticancer efficacy using a dorsal skin-fold window chamber model.

    abstract：AIM:To evaluation the doxorubicin (DOX)-loaded pH-sensitive polymeric micelle release from tumor blood vessels into tumor interstitium using an animal vessel visibility model, the so-called dorsal skin-fold window chamber model. METHODS:DOX-loaded pH-sensitive polyHis-b-PEG micelles and DOX-loaded pH-insensitive PLLA-...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2014.12

    authors： Jin ZH,Jin MJ,Jiang CG,Yin XZ,Jin SX,Quan XQ,Gao ZG

    更新日期：2014-06-01 00:00:00

  • Immunomodulatory effects of lactoferrin.

    abstract：:Lactoferrin (Lf) is an iron-binding glycoprotein of the transferrin family, which is expressed in most biological fluids with particularly high levels in mammalian milk. Its multiple activities lie in its capacity to bind iron and to interact with the molecular and cellular components of hosts and pathogens. Lf can bi...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2013.200

    authors： Siqueiros-Cendón T,Arévalo-Gallegos S,Iglesias-Figueroa BF,García-Montoya IA,Salazar-Martínez J,Rascón-Cruz Q

    更新日期：2014-05-01 00:00:00

  • Oriental herbs as a source of novel anti-androgen and prostate cancer chemopreventive agents.

    abstract：:Androgen and androgen receptor (AR) signaling are crucial for the genesis of prostate cancer (PCa), which can often develop into androgen-ligand-independent diseases that are lethal to the patients. Recent studies show that even these hormone-refractory PCa require ligand-independent AR signaling for survival. As curr...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1111/j.1745-7254.2007.00683.x

    authors： Lu J,Kim SH,Jiang C,Lee H,Guo J

    更新日期：2007-09-01 00:00:00

  • Efficacy-oriented compatibility for component-based Chinese medicine.

    abstract：:Single-target drugs have not achieved satisfactory therapeutic effects for complex diseases involving multiple factors. Instead, innovations in recent drug research and development have revealed the emergence of compound drugs, such as cocktail therapies and "polypills", as the frontier in new drug development. A trad...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1038/aps.2015.8

    authors： Zhang JH,Zhu Y,Fan XH,Zhang BL

    更新日期：2015-06-01 00:00:00

  • Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia.

    abstract：AIM:To study the expressions of vascular endothelial growth factor (VEG F), angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2), Tie-1, and Tie-2 in C57BL/6 mouse brain after permanent focal cerebral ischemia. METHODS:The mRNA levels of VEGF, Ang-1, Ang-2, Tie-1, and Tie-2 were measured by semiquantitative reverse transcri...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Wang RG,Zhu XZ

    更新日期：2002-05-01 00:00:00

  • Discovery of potent beta-secretase (bace-1) inhibitors by the synthesis of isophthalamide-containing hybrids.

    abstract：AIM:The aim of this study was to design and synthesize a series of high activity compounds against aspartyl protease beta-secretase (BACE-1) bearing hydroxyethylene (HE) framework. METHODS:First, we designed the small library based on our previous work and rational analysis. Subsequently, thirteen compounds were selec...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2008.26

    authors： Zhu YP,Xiao K,Yu HP,Ma LP,Xiong B,Zhang HY,Wang X,Li JY,Li J,Shen JK

    更新日期：2009-02-01 00:00:00

  • Resveratrol as a novel agent for treatment of multiple myeloma with matrix metalloproteinase inhibitory activity.

    abstract：AIM:To examine the in vitro antitumor activity of resveratrol against multiple myeloma (MM) cell lines (RPMI 8226, U266, and KM3), and the mechanisms involved. METHODS:The growth inhibition of resveratrol was determined by 3-(4, 5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The effect of resve...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2006.00343.x

    authors： Sun CY,Hu Y,Guo T,Wang HF,Zhang XP,He WJ,Tan H

    更新日期：2006-11-01 00:00:00

  • BCR-ABL1-positive microvesicles malignantly transform human bone marrow mesenchymal stem cells in vitro.

    abstract：:The intercellular communication between leukemia cells and bone marrow mesenchymal stem cells (BM-MSCs) plays more important role in chronic myeloid leukemia (CML) than we previously understood. Recently, we found that microvesicles released from human leukemia cell line K562 (K562-MVs) containing BCR-ABL1 mRNA malign...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2017.116

    authors： Fu FF,Zhu XJ,Wang HX,Zhang LM,Yuan GL,Chen ZC,Li QB

    更新日期：2017-11-01 00:00:00

  • Blocking effect of methylflavonolamine on human Na(V)1.5 channels expressed in Xenopus laevis oocytes and on sodium currents in rabbit ventricular myocytes.

    abstract：AIM:To investigate the blocking effects of methylflavonolamine (MFA) on human Na(V)1.5 channels expressed in Xenopus laevis oocytes and on sodium currents (I(Na)) in rabbit ventricular myocytes. METHODS:Human Na(V)1.5 channels were expressed in Xenopus oocytes and studied using the two-electrode voltage-clamp techniqu...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2010.8

    authors： Fan XR,Ma JH,Zhang PH,Xing JL

    更新日期：2010-03-01 00:00:00

  • Stereoselective metabolism of propafenone by human liver CYP3A4 expressed in transgenic Chinese hamster CHL cells lines.

    abstract：AIM:To investigate the stereoselective metabolism of propafenone (PPF) by human liver CYP3A4. METHODS:A chiral and an achiral HPLC were combined to determine the enantiomer of PPF in S9 incubates prepared from transgenic Chinese hamster CHL cells lines expressing CYP3A4. The time-dependent study was performed using in...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：

    authors： Zhou Q,Yao TW,Yu YN,Zeng S

    更新日期：2001-10-01 00:00:00

  • Sodium hydrosulfide alleviates lung inflammation and cell apoptosis following resuscitated hemorrhagic shock in rats.

    abstract：AIM:To investigate the protective effects of hydrogen sulfide (H2S) against inflammation, oxidative stress and apoptosis in a rat model of resuscitated hemorrhagic shock. METHODS:Hemorrhagic shock was induced in adult male SD rats by drawing blood from the femoral artery for 10 min. The mean arterial pressure was main...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2013.96

    authors： Xu DQ,Gao C,Niu W,Li Y,Wang YX,Gao CJ,Ding Q,Yao LN,Chai W,Li ZC

    更新日期：2013-12-01 00:00:00

  • Shenqi fuzheng injection attenuates irradiation-induced brain injury in mice via inhibition of the NF-κB signaling pathway and microglial activation.

    abstract：AIM:Radiation-induced brain injury (RIBI) is the most common and severe adverse effect induced by cranial radiation therapy (CRT). In the present study, we examined the effects of the traditional Chinese medicine Shenqi Fuzheng Injection (SFI) on RIBI in mice, and explored the underlying mechanisms. METHODS:C57BL/6J m...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2015.69

    authors： Zhang J,Tong F,Cai Q,Chen LJ,Dong JH,Wu G,Dong XR

    更新日期：2015-11-01 00:00:00

  • Anisomycin induces glioma cell death via down-regulation of PP2A catalytic subunit in vitro.

    abstract：AIM:To examine the effects of anisomycin on glioma cells and the related mechanisms in vitro. METHODS:The U251 and U87 human glioblastoma cell lines were tested. The growth of the cells was analyzed using a CCK-8 cell viability assay. Apoptosis was detected using a flow cytometry assay. The expression of proteins and ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2012.46

    authors： Li JY,Huang JY,Li M,Zhang H,Xing B,Chen G,Wei D,Gu PY,Hu WX

    更新日期：2012-07-01 00:00:00

  • Relationship between atrial natriuretic peptide-immunoreactive cells and microvessels in rat gastric mucosa.

    abstract：AIM:To investigate the ultrastructural localization of atrial natriuretic peptide (ANP)-synthesizing cells and the relationship between ANP-synthesizing cells and microvessels in rat gastric mucosa. METHODS:Immunohistochemistry techniques and postembedding immunoelectron microscopy techniques were used to validate the...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2006.00226.x

    authors： Li CH,Yang ZW,Yin ZR,Jin Z,Xing DG,Piao LH,Kim YC,Xu WX

    更新日期：2006-02-01 00:00:00

  • Application of EGFP-EGF fusions to explore mechanism of endocytosis of epidermal growth factor.

    abstract：AIM:To develop a simple method for monitoring protein localization of epidermal growth factor (EGF) in living cells. METHODS:Enhanced green fluorescent protein (EGFP) was used as an autofluorescent tag to label EGF ligands. SDS-PAGE and Western blot analysis were used to detect the expression of the EGFP-tagged EGF (E...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2007.00481.x

    authors： Jiang H,Zhang J,Shi BZ,Xu YH,Li ZH,Gu JR

    更新日期：2007-01-01 00:00:00

  • Perspectives of antimony compounds in oncology.

    abstract：:Antimony, a natural element that has been used as a drug for over more than 100 years, has remarkable therapeutic efficacy in patients with acute promyelocytic leukemia. This review focuses on recent advances in developing antimony anticancer agents with an emphasis on antimony coordination complexes, Sb (III) and Sb ...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章,评审

    doi：10.1111/j.1745-7254.2008.00818.x

    authors： Sharma P,Perez D,Cabrera A,Rosas N,Arias JL

    更新日期：2008-08-01 00:00:00

  • Activation of muscarinic receptors inhibits glutamate-induced GSK-3β overactivation in PC12 cells.

    abstract：AIM:To investigate the actions of the muscarinic agonist carbachol on glutamate-induced neurotoxicity in PC12 cells, and the underlying mechanisms. METHODS:PC12 cells were treated with different concentrations of glutamate for 24 or 48 h. The cell viability was measured using MTT assay, and the expression and activati...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1038/aps.2013.42

    authors： Ma K,Yang LM,Chen HZ,Lu Y

    更新日期：2013-07-01 00:00:00

  • High-throughput real-time assay based on molecular beacons for HIV-1 integrase 3'-processing reaction.

    abstract：AIM:To develop a high-throughput real-time assay based on molecular beacons to monitor the integrase 3'-processing reaction in vitro and apply it to inhibitor screening. METHODS:The recombinant human immunodeficiency virus (HIV)-1 integrase (IN) is incubated with a 38 mer oligonucleotide substrate, a sequence identica...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2007.00561.x

    authors： He HQ,Ma XH,Liu B,Zhang XY,Chen WZ,Wang CX,Cheng SH

    更新日期：2007-06-01 00:00:00

  • CJZ3, a lomerizine derivative, modulates P-glycoprotein function in rat brain microvessel endothelial cells.

    abstract：AIM:To investigate the modulatory effect of CJZ3, a lomerizine derivative, on P-glycoprotein (P-gp) function in rat brain microvessel endothelial cells (RBMEC). METHODS:RBMEC were isolated and cultured in Dulbecco modified Eagle medium/F12 (1:1) medium, and the amount of intracellular rhodamine 123 (Rh123) was determi...

    journal_title：Acta pharmacologica Sinica

    pub_type： 杂志文章

    doi：10.1111/j.1745-7254.2006.00294.x

    authors： Ji BS,He L,Li XQ,Liu GQ

    更新日期：2006-04-01 00:00:00