Utility of GATA-3 in the work-Up of breast adenocarcinoma and its differential diagnosis in serous effusions:: A Cell-Block Microarray Study.

Abstract:

BACKGROUND:GATA-3 is a transcription factor involved in human tissue growth and differentiation. It is a potential marker for breast carcinoma origin in metastasis and predictive of good prognosis. We aim to evaluate the role of GATA3 in determining the breast origin of metastatic adenocarcinoma in malignant effusions using immunohistochemistry on cell-block microarray in comparison with ER and PR results. METHODS:Cell blocks from 100 cases of malignant and reactive serous effusions with confirmed diagnosis were selected; 28 mammary carcinomas, 64 extra-mammary adenocarcinomas (gastrointestinal, pulmonary, gynecologic), and 8 reactive mesothelium proliferation as control. Immunohistochemistry on cell-block microarray was used. Microarray slides were stained for GATA-3, ER and PR. Nuclear staining of >1% was considered positive. RESULTS:GATA3, ER and PR were positive in 25 (89%), 20 (71%) and 16 (57%) of breast carcinoma cases, respectively. All non-breast cancer cases were negative for GATA-3 with the exception of one Müllerian adenocarcinoma (1.6%). The calculated sensitivity, specificity and positive predictive value (PPV) of GATA3 reactivity in determining the breast origin of metastatic adenocarcinoma was 89.3% (95% CI: 71.7-97.7%), 98.6% (95% CI: 91.6-99.9%) and 96.2% (95% CI: 80.4-99.9%), respectively. GATA3 positivity was associated with ER or PR positivity in 84% of cases. CONCLUSIONS:GATA3 is a useful marker in determining the breast origin of metastatic adenocarcinoma in malignant serous effusions. Reactivity to GATA3 may indicate good prognosis. Diagn. Cytopathol. 2016;44:731-736. © 2016 Wiley Periodicals, Inc.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

El Hag MI,Ha J,Farag R,El Hag AM,Michael CW

doi

10.1002/dc.23521

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

731-6

issue

9

eissn

8755-1039

issn

1097-0339

journal_volume

44

pub_type

杂志文章
  • Recurrent respiratory papillomatosis: cytopathological findings in an unusual case.

    abstract::Recurrent respiratory papillomatosis (RRP) primarily is a disease of children and adolescents. Most patients experience spontaneous regression at a later age, while others continue to be affected throughout their lives. Here, we present the case of a 26-yr-old man who presented with persistent cough, worsening dyspnea...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20134

    authors: Parwani AV,Sheth S,Ali SZ

    更新日期:2004-12-01 00:00:00

  • Unusual presentation of disseminated histoplasmosis in an immunocompetent patient.

    abstract::Disseminated histoplasmosis (DH) is a systemic granulomatous disease caused by the fungus Histoplasma capsulatum. This disease occurs mainly in immunocompromised individuals with CD 4 counts less than 200 cell/µL and is rarely noted in immunocompetent person. In India, this disease has been reported from several parts...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23742

    authors: Kumari M,Udayakumar M,Kaushal M,Madaan GB

    更新日期:2017-09-01 00:00:00

  • Cultured anaplastic cell lines as immunocytochemistry controls: a comparison of ThinPrep-processed smears and conventional air-dried cytospins.

    abstract::Cultured anaplastic cell lines with previously characterized phenotypes are considered to be the best positive controls for immunocytochemistry. We assessed the validity of using anaplastic cell line cytospins as positive controls for immunocytochemistry performed on ThinPrep-processed clinical samples. We compared Th...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2059

    authors: Wiatrowska BA,Berner A,Torlakovic G,Emilsen E,Mykelbost GL,Torlakovic E

    更新日期:2001-11-01 00:00:00

  • Diagnosis of histoplasmosis in urine cytology: reactive urothelial changes, a diagnostic pitfall. Case report and literature review of urinary tract infections.

    abstract::Histoplasmosis not uncommonly causes systemic infection, particularly in immunocompromised patients. In systemic infection, the urinary tract is often involved, although the diagnosis of histoplasmosis in urine cytologic specimens has never been reported. Urinary tract histoplasmosis may present with gross hematuria, ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10049

    authors: Mukunyadzi P,Johnson M,Wyble JG,Scott M

    更新日期:2002-04-01 00:00:00

  • Bilateral primary lymphoma of the breast: a case report initially diagnosed by FNAC.

    abstract::Primary lymphoma of the breast (PLB) is a rare disease, representing 0.04-0.5% of all malignant breast neoplasms. We present a patient with bilateral breast involvement by a high-grade diffuse large B-cell lymphoma, which was diagnosed initially by fine-needle aspiration cytology (FNAC). Mammography revealed a diffuse...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20184

    authors: Maounis N,Ellina E,Papadaki T,Ioannides G,Kiale K,Finokaliotis N,Blana AK

    更新日期:2005-02-01 00:00:00

  • Fine-needle aspiration of breast lesions: role and accuracy in a review of 7,495 cases.

    abstract::In the past 10 years, 7,495 cytological breast fine-needle aspirations (FNAs) were performed (4,756 FNAs of solid nodes and 2,739 of cystic nodes). Of these, 2,099 cases underwent surgery; 650 (31%) had histologically proven carcinoma. Sensitivity was 83.9%, specificity was 99.5%, the predictive value for negative res...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840030206

    authors: Fessia L,Botta G,Arisio R,Verga M,Aimone V

    更新日期:1987-06-01 00:00:00

  • Pregnancy-associated anaplastic large-cell lymphoma of the breast: a rare mimic of ductal carcinoma.

    abstract::Anaplastic large-cell lymphoma (ALCL) is a rare T-cell lymphoma typically seen in children and young adults. It has been described in numerous sites; however, the breast is one of the least common locations. We herein report a case of ALCL arising in the breast of a 36-yr-old pregnant woman. To our knowledge this is t...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20442

    authors: Iyengar P,Reid-Nicholson M,Moreira AL

    更新日期:2006-04-01 00:00:00

  • Utility of cell blocks in the diagnosis of thyroid aspirates.

    abstract::Cell blocks (CBs) are often prepared with fine-needle aspirates (FNAs) from multiple organs as an adjunct to smears in the diagnosis of aspirated lesions. However, the literature contains few reports on their utility with regard to specific organ sites. At our institution, CBs are made routinely on FNAs when there is ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20385

    authors: Sanchez N,Selvaggi SM

    更新日期:2006-02-01 00:00:00

  • The diagnostic utility of cell block in fine needle aspiration cytology of palpable breast lesions in a Nigerian tertiary health institution.

    abstract:BACKGROUND:Although fine needle aspiration cytology (FNAC) is highly accurate for detecting breast malignancies, concerns remain among cytopathologists about false-positive and false-negative diagnoses. Cell block (CB) preparations have been advocated by some cytopathologists as one of the methods to improve and consol...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24576

    authors: Obiajulu FJN,Daramola AO,Anunobi CC,Ikeri NZ,Abdulkareem FB,Banjo AA

    更新日期:2020-12-01 00:00:00

  • Ciliated muconodular papillary tumors of the lung: Cytologic features and diagnostic pitfalls in intraoperative examinations.

    abstract::Ciliated muconodular papillary tumors (CMPTs) of the lung are rare, likely benign neoplastic lesions. Here we describe a case of a CMPT, focusing on its cytologic features, which to our knowledge have not been reported previously. Owing to dull back pain, a 69-year-old male non-smoker underwent CT, which revealed a 1....

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24169

    authors: Mikubo M,Maruyama R,Kakinuma H,Yoshida T,Satoh Y

    更新日期:2019-07-01 00:00:00

  • Evaluation of flow cytometric immunophenotyping and DNA analysis for detection of malignant cells in serosal cavity fluids.

    abstract::The serosal cavities are frequent sites of tumor metastasis. The distinction between carcinoma cells, inflammatory cells, and reactive or malignant mesothelial cells can be difficult in cytology. Multicolor flow cytometry (FCM) provides the opportunity to evaluate multiple antigens simultaneously, making it possible t...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21047

    authors: Sayed DM,el-Attar MM,Hussein AA

    更新日期:2009-07-01 00:00:00

  • GATA3 expression in metastatic urothelial carcinoma in fine needle aspiration cell blocks: a review of 25 cases.

    abstract::GATA binding protein 3 is a zinc finger transcription factor with high affinity for urothelial tissue and is a promising immunohistochemical marker in detection of urothelial carcinomas (UC). We studied its usefulness in metastatic high-grade UC. This study was performed on cell blocks (CB) of fine needle aspirates fr...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23131

    authors: Siddiqui MT,Seydafkan S,Cohen C

    更新日期:2014-09-01 00:00:00

  • Cytomorphology of giant cell glioblastoma: Report of a case and brief review of literature.

    abstract::Giant cell glioblastoma is a histological variant of glioblastoma that accounts for less than 1% of intracranial tumors and to 5% of glioblastoma. They occur at any age and are likely to affect the younger as well the older age group unlike the conventional glioblastoma multiforme (GBM). They are often located subcort...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.21648

    authors: Jaiswal S,Vij M,Jaiswal AK,Srivastava AK,Behari S,Pandey R

    更新日期:2012-05-01 00:00:00

  • The cytopathology of mycobacterial infection.

    abstract::Mycobacterial infection, tuberculosis (TB) in particular, remains one of the world's deadliest communicable diseases in adults and particularly in children, in low and middle income countries. The combination of human immunodeficiency virus (HIV) and TB is often lethal with TB accounting for 25% of deaths in the HIV p...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.23410

    authors: Michelow P,Omar T,Field A,Wright C

    更新日期:2016-03-01 00:00:00

  • Inspissated cyst fluid in endoscopic ultrasound-guided fine needle aspiration of pancreatic cysts.

    abstract:BACKGROUND:Inspissated cyst fluid may be identified on pancreatic cyst aspiration cytology. We report on the cytomorphologic characteristics of inspissated cyst fluid on EUS-FNA of pancreatic cysts and correlate this finding with histopathology or multimodal (cytology, cyst fluid analysis, molecular pathology, imaging)...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23912

    authors: Chebib I,Albanese E,Scourtas A,Pitman MB

    更新日期:2018-05-01 00:00:00

  • Mites in pulmonary cytology specimens.

    abstract::Mites were observed in 55 pulmonary specimens examined routinely over a 4.5-yr period. The 55 specimens included 33 spontaneous and 12 aerosol-induced sputum samples, nine bronchial washings, and one fine-needle aspirate. Two specimens contained only fragments of mites; however, in most cases the mites were relatively...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840050416

    authors: Farley ML,Mabry LC,Hieger LR

    更新日期:1989-01-01 00:00:00

  • Fine-needle aspiration cytology in the diagnosis of superficial lymphadenopathy: a 5-year Brazilian experience.

    abstract::To determine the accuracy of fine-needle aspiration (FNA) in the diagnostic assessment of lymphadenopathies, a retrospective study was conducted on 627 cases of FNA of the lymph nodes performed at Department of Pathology, UNIFESP (Federal University of São Paulo), between 1997 and 2001. Cytology results were compared ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20373

    authors: Martins MR,Santos Gda C

    更新日期:2006-02-01 00:00:00

  • Cytology of sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease).

    abstract::Sinus histiocytosis with massive lymphadenopathy (SHML) is a benign, self-limiting condition of unknown etiology, which generally presents as massive bilateral cervical lymphadenopathy. It is important to distinguish SHML from other causes of histiocytosis because of the different treatment modalities. This study was ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(20000301)22:3<181::aid-dc1

    authors: Deshpande AH,Nayak S,Munshi MM

    更新日期:2000-03-01 00:00:00

  • Detection of apoptotic cells in cytology specimens: an application of TdT-mediated dUTP-biotin nick end labeling to cell smears.

    abstract::We applied TdT-mediated deoxyuridine triphosphate (dUTP)-biotin nick end labeling (TUNEL) to cytologic smears in order to detect the cells undergoing apoptosis. These smears were obtained by scraping the cut surface of 9 cases of carcinoma, including renal-cell carcinoma (3 cases), esophageal squamous-cell carcinoma (...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199806)18:6<398::aid-dc3>3

    authors: Sasano H,Yamaki H,Nagura H

    更新日期:1998-06-01 00:00:00

  • Pleomorphic adenoma with bizarre myoepithelial cells: A diagnostic challenge on cytologic smear.

    abstract::The diagnosis on fine needle aspiration of salivary gland tumors with a myoepithelial component is challenging because myoepithelial cells can have a wide cytomorphologic spectrum. The authors report a case of a pleomorphic adenoma of the parotid gland that expands the spectrum of appearances that myoepithelial cells ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24301

    authors: Taweevisit M,Thorner PS

    更新日期:2019-12-01 00:00:00

  • Cytology of sclerosing epithelioid fibrosarcoma in pleural effusion.

    abstract::We report a case of sclerosing epithelioid fibrosarcoma focusing on its cytological features in pleural effusion. A 32-year-old man had noticed a tumor in his left buttock 5 years earlier but had not sought treatment because the tumor had been painless. He visited our hospital because the tumor had gradually increased...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21310

    authors: Tsuchido K,Yamada M,Satou T,Otsuki Y,Shimizu S,Kobayashi H

    更新日期:2010-10-01 00:00:00

  • Primary pancreatic leiomyosarcoma with metastasis to the liver diagnosed by endoscopic ultrasound-guided fine needle aspiration and fine needle biopsy: A Case Report and Review of Literature.

    abstract::Primary pancreatic leiomyosarcomas are rare tumors of the pancreas that are usually diagnosed after resection or by biopsy. One case in the literature has utilized endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) cytology. We report a second case of a primary pancreatic leiomyosarcoma that yielded diagnos...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/dc.23540

    authors: Reyes MC,Huang X,Bain A,Ylagan L

    更新日期:2016-12-01 00:00:00

  • Cytological typing of primary lung cancer: study of 100 cases with autopsy confirmation.

    abstract::In order to assess the accuracy of bronchial aspiration cytology in typing lung cancer, tissue sections from 100 autopsy cases of lung cancer were compared with the cytology features observed in the same patients prior to death. There was 100% accuracy in the cytology of small-cell carcinoma; 90% in squamous-cell carc...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840070104

    authors: DiBonito L,Colautti I,Patriarca S,Falconieri G,Barbazza R,Vielh P

    更新日期:1991-01-01 00:00:00

  • Cytologic diagnosis of true thymic hyperplasia by combined radiologic imaging and aspiration cytology: a case report including flow cytometric analysis.

    abstract::True thymic hyperplasia (TH) is an age-dependent increase in size and weight of the thymus gland, which by definition maintains a normal histologic architecture. TH can mimic other important diseases, including lymphofollicular hyperplasia, thymoma, lymphoma, and germ-cell tumors. Traditionally, separating these entit...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/1097-0339(200012)23:6<417::aid-dc12>3.0.co

    authors: Hoerl HD,Wojtowycz M,Gallagher HA,Kurtycz DF

    更新日期:2000-12-01 00:00:00

  • Cytology, flow cytometry, image analysis, and interphase cytogenetics by fluorescence in situ hybridization in the diagnosis of transitional cell carcinoma in bladder washes: a comparative study.

    abstract::The diagnosis of transitional cell carcinoma (TCC) in bladder washes is a diagnostic challenge to cytology. This study assessed the role of flow cytometry (FCM), image analysis (IA), and interphase cytogenetics by fluorescence in situ hybridization (FISH) as adjuncts in the cytodiagnosis of TCC in bladder washes. Fort...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840130307

    authors: Cajulis RS,Haines GK 3rd,Frias-Hidvegi D,McVary K,Bacus JW

    更新日期:1995-10-01 00:00:00

  • Utility of fine-needle aspiration cytology in the classification of leprosy.

    abstract::The role of fine-needle aspiration cytology (FNAC) in the diagnosis of benign skin lesions has been restricted primarily to the evaluation of bacteriologic and morphologic indices in leprosy. This study was undertaken to evaluate the efficacy of FNAC in the diagnosis and classification of lepromatous lesions. Aspirate...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.1068

    authors: Rao IS,Singh MK,Gupta SD,Pandhi RK,Kapila K

    更新日期:2001-05-01 00:00:00

  • Cytologic study of renal angiomyolipoma by fine-needle aspiration biopsy: report of four cases.

    abstract::Four cases of renal angiomyolipoma (AML) diagnosed by fine-needle aspiration biopsy (FNAB) are reported. One case was associated with the clinical complex of tuberous sclerosis. The tumors were solitary in two cases and multiple and bilateral in the fourth. The cytologic features of the four cases were similar to thos...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840100110

    authors: Tallada N,Martinez S,Raventos A

    更新日期:1994-01-01 00:00:00

  • Effectiveness of AutoPap system location-guided screening in the evaluation of cervical cytology smears.

    abstract::Location-guided screening is a feature of the AutoPap primary screening software. Areas of the smear most likely to contain an abnormality are identified for prompt review by the cytotechnologist, thereby facilitating diagnostic accuracy and reducing laboratory workload. A two-armed retrospective study comprising 6,00...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20081

    authors: Stevens MW,Milne AJ,Parkinson IH,Nespolon WW,Fazzalari NL,Arora N,Dodd TJ

    更新日期:2004-08-01 00:00:00

  • Cytologic findings in microglandular hyperplasia of the cervix.

    abstract::A case of florid microglandular hyperplasia of the uterine cervix that contained cells with severe atypia suggesting malignancy on a Papanicolaou smear is reviewed. Findings from 25 additional smears from patients with microglandular hyperplasia are reported. These sometimes contained glandular cells with varying degr...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840070308

    authors: Yahr LJ,Lee KR

    更新日期:1991-01-01 00:00:00

  • Fine needle aspiration of secondary synovial sarcoma of the thyroid gland.

    abstract::Synovial sarcomas (SS) of the head and neck region are extremely rare and arise in only 5% of cases. We present a case of secondary SS of the thyroid originally diagnosed as medullary carcinoma on fine needle aspiration (FNA). A 41-year-old man presented with several weeks of dysphonia and a left thyroid mass. FNA of ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23327

    authors: Murro D,Slade JM,Syed S,Gattuso P

    更新日期:2015-11-01 00:00:00