Evaluation of flow cytometric immunophenotyping and DNA analysis for detection of malignant cells in serosal cavity fluids.

Abstract:

:The serosal cavities are frequent sites of tumor metastasis. The distinction between carcinoma cells, inflammatory cells, and reactive or malignant mesothelial cells can be difficult in cytology. Multicolor flow cytometry (FCM) provides the opportunity to evaluate multiple antigens simultaneously, making it possible to characterize various cell populations. In this study, we aimed to assess the diagnostic accuracy of FCM immunophenotyping and DNA in comparison with serum tumor markers and classic cytology for detection of malignant cells in pleural and ascitic fluids. One hundred and nineteen samples of body cavity fluids were analyzed. Immunophenotyping was performed by four-color immunofluorescent staining using monoclonal antibodies against Ber-EP4, cytokeratin, CD3, and CD45. The DNA analysis by FCM was also performed. In addition, serum CA19-9, CEA, AFP, and CA125 were analyzed. Ber-EP4 marker had the highest sensitivity (73%) and specificity (95.5%) in the detection of carcinoma cells in serous fluid and correlated with cytology in most of cases (73%). The mean of DI differed statistically in patients with malignant effusions than in benign one. DI showed no difference in fluids due to infiltration of malignant epithelial cells or hematopoietic malignancy or due to hepatocellular carcinoma developing in cirrhotic liver. Thus, flow cytometry appears to aid not only in the detection of malignant cells but also in the characterization of cell type. On the other hand, although DNA ploidy examination had better sensitivity; it had no advantage over conventional cytopathological examination in identification of malignant cells.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Sayed DM,el-Attar MM,Hussein AA

doi

10.1002/dc.21047

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

498-504

issue

7

eissn

8755-1039

issn

1097-0339

journal_volume

37

pub_type

杂志文章
  • Ultrasound-guided fine-needle aspiration biopsy remains a valid approach in the evaluation of nonpalpable breast lesions.

    abstract::The use of ultrasound-guided fine-needle aspiration (FNA) biopsy for nonpalpable breast lesions varies considerably. This retrospective study stresses the role of breast FNA in evaluating sonographically suspicious nonpalpable breast masses using a probabilistic reporting system. One hundred and eight consecutive ultr...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20068

    authors: Liao J,Davey DD,Warren G,Davis J,Moore AR,Samayoa LM

    更新日期:2004-05-01 00:00:00

  • Papanicolaou's nebular cells are related to HPV infection and HSIL.

    abstract::Metaplastic cells with nebular cytoplasmic changes in the cervical smear are classified in the Dutch coding system for cervical screening as KOPAC O8 cells. Since these nebulated cells are already documented by Papanicolaou, we refer to these cells as Papanicolaou's nebular cells. We examined the simultaneous presence...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21523

    authors: Roeters AM,Boon ME,van Haaften M,Vernooij F,Bontekoe TR,Heintz AP

    更新日期:2012-03-01 00:00:00

  • Combining fine needle aspiration with brushing cytology has improved yields in diagnosing pancreatic ductal adenocarcinoma.

    abstract::The aim of this retrospective study is to evaluate the diagnostic yields of combining fine needle aspiration (FNA) with brushing cytology (BC) in clinical work-up of pancreatic ductal adenocarcinoma. The study included a total of 97 patients who underwent both FNA and BC along with histologic/clinical follow-up (F/U)....

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21062

    authors: Jing X,Wamsteker EJ,Li H,Pu RT

    更新日期:2009-08-01 00:00:00

  • Barr body in fine needle aspiration cytology of ovarian malignancies.

    abstract::The Barr body is the inactive X chromosome in a female somatic cell. It is readily identified as plano-convex structure of 2-3 micron in diameter on the periphery of the nuclear membrane. The aim of this study is to evaluate the significance of Barr body count in malignant ovarian tumors on fine needle aspiration cyto...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21688

    authors: Agrawal P,Dey P

    更新日期:2012-11-01 00:00:00

  • Fine-needle aspiration of primary gallbladder carcinoma.

    abstract::Primary carcinoma of the gallbladder is a rare neoplasm that is frequently difficult to diagnose preoperatively. The obstacles to diagnosis include vague symptoms and the relative inaccessibility of the gallbladder and cystic duct to biopsy. Cytological descriptions of gallbladder carcinoma are sparse and are largely ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0339(199608)15:2<151::AID-DC14>

    authors: Dodd LG,Moffatt EJ,Hudson ER,Layfield LJ

    更新日期:1996-08-01 00:00:00

  • Osteitis fibrosa cystica (brown tumor) of the spine with cord compression: report of a case with needle aspiration biopsy findings.

    abstract::A case of osteitis fibrosa cystica or brown tumor of bone in a patient presenting with acute spinal cord compression that was suggested initially by needle aspiration biopsy of the spine is described. Following the aspiration biopsy, excision of vertebral lesions, cord decompression, and spinal fusion were successfull...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840060512

    authors: Kashkari S,Kelly TR,Bethem D,Pepe RG

    更新日期:1990-01-01 00:00:00

  • Bleeding from Wharton's duct after fine-needle aspiration biopsy on submandibular gland.

    abstract::The case of a fine-needle aspiration biopsy (FNAB) of the patient's salivary gland lesions is considered a rapid, simple, reliable, minimal invasive procedure with markedly rare complications. Herein, we report a case of bleeding from the region of the Wharton's duct after a procedure of a FNAB on the patient's subman...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24030

    authors: Lee DH,Yoon TM,Lee JK,Lim SC

    更新日期:2018-11-01 00:00:00

  • Fine-needle aspiration cytology and immunocytochemistry of orbital masses.

    abstract::A series of 85 fine-needle aspiration (FNA) biopsies from orbital space occupying lesions of 82 patients are reviewed. A total of 32 benign lesions and 49 malignant lesions were conclusively diagnosed. In two cases the aspirates were insufficient for diagnosis. Of two cases, which were cytologically suspicious for lym...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20375

    authors: Tani E,Seregard S,Rupp G,Söderlund V,Skoog L

    更新日期:2006-01-01 00:00:00

  • Bladder-washing cytology of metastatic ovarian granulosa cell tumor.

    abstract::An 86-year-old Caucasian female presented with two weeks history of discomfort discharging urine, occasional hematuria, and suprapubic pain. The patient had a history of left salpingo-oophorectomy for an ovarian tumor, performed four years earlier. Ultrasound showed a solid mass surrounding the orifice of the left ure...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.10095

    authors: Vodovnik A

    更新日期:2002-06-01 00:00:00

  • Pericardial effusion as primary manifestation of metastatic cutaneous adenoid cystic carcinoma: diagnostic cytopathology from an exfoliative sample.

    abstract::Adenoid cystic carcinoma (ACC) occurs not only as a tumor of salivary glands, but also in very unusual locations, such as in the skin. Only very few cases of primary cutaneous of ACC have metastasized to the lymph nodes and lungs. We present a 53-year-old man with metastasis of the pericardium from a primary cutaneous...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20818

    authors: Benchetritt M,Butori C,Long E,Ilie M,Ferrari E,Hofman P

    更新日期:2008-05-01 00:00:00

  • A case of endocervical minimal deviation adenocarcinoma with varicolored cytopathologic features on Pap smear.

    abstract::We report a case of minimal deviation adenocarcinoma (MDA) of the uterine cervix showing varicolored cytopathologic features on Papanicolaou (Pap) smear, which may indicate features suggestive of lobular endocervical glandular hyperplasia (LEGH)-adenocarcinoma sequence. A 57-year-old woman presented with a profuse amo...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.23932

    authors: Omori M,Kondo T,Nakazawa K,Shinohara S,Watanabe Y,Fukasawa H,Nakazawa T,Hashi A,Hirata S

    更新日期:2018-08-01 00:00:00

  • The Bethesda System for Reporting Thyroid Cytology (TBSRTC): From look-backs to look-ahead.

    abstract::The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) was formalized in October 2007 by experts in thyroidology at the National Institute of Health in Bethesda, Maryland. The first edition of the TBSRTC book was published in 2010 and the second edition in 2018. The TBSRTC is widely employed in cytology prac...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24385

    authors: Baloch Z,LiVolsi VA

    更新日期:2020-10-01 00:00:00

  • Clinical evaluation, imaging studies, indications for cytologic study, and preprocedural requirements for duct brushing studies and pancreatic FNA: the Papanicolaou Society of Cytopathology recommendations for pancreatic and biliary cytology.

    abstract::The Papanicolaou Society of Cytopathology has developed a set of guidelines for pancreaticobiliary cytology including indications for endoscopic ultrasound (EUS) and fine-needle aspiration (FNA) biopsy, techniques for EUS-FNA, terminology and nomenclature to be used for pancreaticobiliary disease, ancillary testing, a...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,实务指引

    doi:10.1002/dc.23095

    authors: Adler D,Max Schmidt C,Al-Haddad M,Barthel JS,Ljung BM,Merchant NB,Romagnuolo J,Shaaban AM,Simeone D,Bishop Pitman M,Field A,Layfield LJ,Papanicolaou Society of Cytopathology.

    更新日期:2014-04-01 00:00:00

  • Axillary lymph node metastasis: Fine-needle aspiration biopsy sensitivity as a function of node size, percent replacement of lymph node by tumor and tumor deposit size.

    abstract:BACKGROUND:Fine-needle aspiration (FNA) is commonly used to investigate lymphadenopathy of suspected metastatic origin. While diagnostic accuracy of FNA for lymph node disease is well described, the relationship between node size, percent tumor replacement, and size of metastatic deposit with diagnostic accuracy is les...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24617

    authors: Layfield LJ,Zhang T,Esebua M,Schmidt R

    更新日期:2021-02-01 00:00:00

  • Fascin stain as a potential marker of invasiveness in carcinomas of the urinary bladder: a retrospective study with biopsy and cytology correlation.

    abstract::The evaluation of invasion in urothelial carcinomas of the urinary bladder cannot be determined on cytology and can be particularly challenging in biopsy cases with limited sampling. Recent studies of bladder resection specimens suggest that fascin overexpression may be a marker of aggressive urothelial carcinomas and...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21429

    authors: McKnight R,Cohen C,Siddiqui MT

    更新日期:2011-09-01 00:00:00

  • Clinical performance of the aptima HPV assay in 4196 women with positive high-risk HPV and ASC-US cytology: A large women hospital experience.

    abstract:BACKGROUND:Despite Aptima assay as the latest US Food Drug Administration (FDA)-approved high-risk human papillomavirus (hrHPV) test has been implemented as an adjunct in cervical cancer screening for years, histological follow-up data remain limited with respect to its performance in women with ASC-US Pap tests and po...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.24592

    authors: Wang T,Pradhan D,Zhang H,Matsko J,Zhao C

    更新日期:2021-01-01 00:00:00

  • Fine-needle aspiration of supraclavicular lymph nodes.

    abstract::Malignancies from many primary sites may metastasize to supraclavicular lymph nodes (SCLN). We reviewed 100 fine-needle aspirations (FNAs) of SCLNs. There were three major types of malignancy detected by this method: adenocarcinoma (n = 40), squamous cell carcinoma (n = 14), and other malignancies (n = 29). Adenocarci...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-0339(199604)14:3<216::AID-DC4>3

    authors: Carson HJ,Candel AG,Gattuso P,Castelli MJ

    更新日期:1996-05-01 00:00:00

  • Nodular sclerosing classical Hodgkin lymphoma masquerading as acute suppurative-necrotizing lymphadenitis.

    abstract::The diagnosis of nodular sclerosing classical Hodgkin lymphoma (NSCHL) by fine-needle aspiration (FNA) biopsy has historically been a diagnostic challenge due to the usual paucicellularity of the specimen. This case report, and other previously published reports, suggests that there is another facet to the potentially...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21829

    authors: Florentine BD,Cohen AN

    更新日期:2014-03-01 00:00:00

  • Motile ciliary microorganisms in peritoneal fluid.

    abstract::We report a patient with chronic renal failure and ciliocytophthoria or "detached ciliary tufts" identified from her peritoneal fluid. The recognition of these rare structures is critical to avoid misdiagnosing a presumed protozoan infection and embarking on further costly investigations and unnecessary treatments. ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21505

    authors: Vila M,Thompson K,Erdem G

    更新日期:2011-08-01 00:00:00

  • Fine-needle aspiration of breast lesions: role and accuracy in a review of 7,495 cases.

    abstract::In the past 10 years, 7,495 cytological breast fine-needle aspirations (FNAs) were performed (4,756 FNAs of solid nodes and 2,739 of cystic nodes). Of these, 2,099 cases underwent surgery; 650 (31%) had histologically proven carcinoma. Sensitivity was 83.9%, specificity was 99.5%, the predictive value for negative res...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840030206

    authors: Fessia L,Botta G,Arisio R,Verga M,Aimone V

    更新日期:1987-06-01 00:00:00

  • Metastatic, sarcomatoid, and PSA- and PAP-negative prostatic carcinoma: diagnosis by fine-needle aspiration.

    abstract::Fine-needle aspiration of prostatic carcinoma usually yields an acinar carcinoma that is immunoreactive for prostatic-specific antigen (PSA) and prostatic acid phosphatase (PAP). We report on two FNAs of metastatic sarcomatoid prostatic carcinoma that were PSA- and PAP-negative. Our methods included a review of the me...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/1097-0339(200009)23:3<199::aid-dc12>3.0.co

    authors: Renshaw AA,Granter SR

    更新日期:2000-09-01 00:00:00

  • The aspiration cytology of pulmonary hamartomas.

    abstract::Cytodiagnosis of pulmonary hamartomas (PHs) by transthoracic fine-needle aspiration (FNA), successful in 11 of a series of 14 cases, has been found to rely less on the presence of mature cartilage than on the recognition of fibromyxoid fragments. These fragments, wispy yet discrete, consisting of variable numbers of f...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840050212

    authors: Dunbar F,Leiman G

    更新日期:1989-01-01 00:00:00

  • Fine-needle aspiration cytology of salivary duct carcinoma.

    abstract::Salivary duct carcinoma (SDC) is an extremely rare and highly aggressive primary epithelial neoplasm of the salivary glands associated with increased tendency for lymph node and vascular metastases. Histologically, this lesion resembles intraductal cribriform and comedo carcinoma of the breast. Fine-needle aspiration ...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2840110111

    authors: Elsheikh TM,Bernacki EG Jr,Pisharodi L

    更新日期:1994-01-01 00:00:00

  • Chondroid syringoma diagnosed by fine needle aspiration cytology.

    abstract::Chondroid syringoma is a rare benign skin adnexal tumor of eccrine/apocrine origin affecting commonly the head and neck region. It used to be previously called as mixed tumor of skin because of the presence of both the epithelial and mesenchymal components. There are only few case reports describing the fine needle as...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21159

    authors: Kumar B

    更新日期:2010-01-01 00:00:00

  • Concurrent pleural infiltration by chronic lymphocytic leukemia and adenocarcinoma of unknown primary site diagnosed by effusion cytology.

    abstract::Synchronous malignancies in a pleural effusion are rare. A case of concurrent pleural infiltration by adenocarcinoma of unknown primary site and chronic lymphocytic leukemia (CLL) is presented in this case study, which was diagnosed by effusion cytology. Pleural effusion is not an uncommon complication in patients wit...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.22889

    authors: Vrettos I,Kamposioras K,Peridis S,Aninos D,Kazika S,Spathis A,Karakitsos P,Papadopoulos A

    更新日期:2014-02-01 00:00:00

  • Cytologic diagnosis of salivary duct carcinoma: a review of seven cases.

    abstract::The cytomorphologic findings in fine-needle aspirates from 7 cases of salivary duct carcinoma (SDC) are reviewed and correlated with the histologic features. Malignant cytologic characteristics are clear in this tumor, and no false-negative results were obtained. But the absence of cribriform or papillary groups sugge...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-0339(199808)19:2<120::aid-dc11>

    authors: García-Bonafé M,Catala I,Tarragona J,Tallada N

    更新日期:1998-08-01 00:00:00

  • Follicular variant of papillary carcinoma: cytologic findings on FNAB samples-experience with 16 cases.

    abstract::Between January 1, 1992 and December 31, 1997, a cytopathological diagnosis of follicular variant of papillary thyroid carcinoma (FVPC) was made on a series of 16 out of 18 patients with palpable nodules who underwent fine-needle aspiration biopsy (FNAB) in our Department. The results of aspiration biopsy were followe...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.2010

    authors: Fulciniti F,Benincasa G,Vetrani A,Palombini L

    更新日期:2001-08-01 00:00:00

  • Unusual presentation of granulocytic sarcoma in the breast: a case report and review of the literature.

    abstract::This is a case of granulocytic sarcoma presenting as bilateral breast masses in a 40-yr-old woman with concurrent unsuspected chronic myeloid leukemia diagnosed by fine-needle aspiration. The granulocytic differentiation was recognized on Diff-Quik-stained cytology smears and confirmed rapidly on flow cytometry on the...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章,评审

    doi:10.1002/1097-0339(200101)24:1<53::aid-dc1009>3.0.c

    authors: Ngu IW,Sinclair EC,Greenaway S,Greenberg ML

    更新日期:2001-01-01 00:00:00

  • Effectiveness of AutoPap system location-guided screening in the evaluation of cervical cytology smears.

    abstract::Location-guided screening is a feature of the AutoPap primary screening software. Areas of the smear most likely to contain an abnormality are identified for prompt review by the cytotechnologist, thereby facilitating diagnostic accuracy and reducing laboratory workload. A two-armed retrospective study comprising 6,00...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.20081

    authors: Stevens MW,Milne AJ,Parkinson IH,Nespolon WW,Fazzalari NL,Arora N,Dodd TJ

    更新日期:2004-08-01 00:00:00

  • Parathyroid fine-needle aspiration cytology in the evaluation of parathyroid adenoma: cytologic findings from 53 patients.

    abstract::This study was designed to assess the utility of fine-needle aspiration cytology (FNAC) in the preoperative localization of parathyroid adenoma (PA). Fifty-seven samples from fifty-three cases of PA (four patients had bilateral disease) were obtained by ultrasound (US)-guided fine-needle aspiration. Parathyroid hormon...

    journal_title:Diagnostic cytopathology

    pub_type: 杂志文章

    doi:10.1002/dc.21020

    authors: Agarwal AM,Bentz JS,Hungerford R,Abraham D

    更新日期:2009-06-01 00:00:00