Abstract:
:There are estimated to be >300,000 plant species, producing >200,000 metabolites. Many of these metabolites are restricted to specific plant lineages and are referred to as "specialized" metabolites. These serve varied functions in plants including defense against biotic and abiotic stresses, plant-plant and plant-microbe communication, and pollinator attraction. These compounds also have important applications in agriculture, medicine, skin care, and in diverse aspects of human culture. The specialized metabolic repertoire of plants can vary even within and between closely related species, in terms of the number and classes of specialized metabolites as well as their structural variants. This phenotypic variation can be exploited to discover the underlying variation in the metabolic enzymes. We describe approaches for using the diversity of specialized metabolites and variation in enzyme structure and function to identify novel enzymatic activities and understand the structural basis for these differences. The knowledge obtained from these studies will provide new modules for the synthetic biology toolbox.
journal_name
Methods Enzymoljournal_title
Methods in enzymologyauthors
Fan P,Moghe GD,Last RLdoi
10.1016/bs.mie.2016.02.023subject
Has Abstractpub_date
2016-01-01 00:00:00pages
1-17eissn
0076-6879issn
1557-7988pii
S0076-6879(16)00107-5journal_volume
576pub_type
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journal_title:Methods in enzymology
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pub_type: 杂志文章
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