A Screening Method for the Isolation of Polyhydroxyalkanoate-Producing Purple Non-sulfur Photosynthetic Bacteria from Natural Seawater.

Abstract:

:Polyhydroxyalkanoates (PHAs) are a family of biopolyesters accumulated by a variety of microorganisms as carbon and energy storage under starvation conditions. We focused on marine purple non-sulfur photosynthetic bacteria as host microorganisms for PHA production and developed a method for their isolation from natural seawater. To identify novel PHA-producing marine purple non-sulfur photosynthetic bacteria, natural seawaters were cultured in nutrient-rich medium for purple non-sulfur photosynthetic bacteria, and twelve pink- or red-pigmented colonies were picked up. Gas chromatography mass spectrometry analysis revealed that four isolates synthesized PHA at levels ranging from 0.5 to 24.4 wt% of cell dry weight. The 16S ribosomal RNA sequence analysis revealed that one isolate (HM2) showed 100% identity to marine purple non-sulfur photosynthetic bacteria. In conclusion, we have demonstrated in this study that PHA-producing marine purple non-sulfur photosynthetic bacteria can be isolated from natural seawater under nutrient-rich conditions.

journal_name

Front Microbiol

authors

Higuchi-Takeuchi M,Morisaki K,Numata K

doi

10.3389/fmicb.2016.01509

subject

Has Abstract

pub_date

2016-09-21 00:00:00

pages

1509

issn

1664-302X

journal_volume

7

pub_type

杂志文章
  • Characterization of Novel Reassortant Influenza A (H5N2) Viruses Isolated from Poultry in Eastern China, 2015.

    abstract::Recently, novel variants of H5 highly pathogenic avian influenza viruses (AIVs) have been frequently isolated from poultry and wild birds in Asia, Europe and North America. Live poultry markets (LPMs) play an important role in the dissemination of influenza viruses. Four H5N2 AIVs were isolated from poultry during sur...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00741

    authors: Wu H,Lu R,Peng X,Peng X,Cheng L,Liu F,Wu N

    更新日期:2017-04-25 00:00:00

  • The Global Regulators Lrp, LeuO, and HexA Control Secondary Metabolism in Entomopathogenic Bacteria.

    abstract::Photorhabdus luminescens TTO1 and Xenorhabdus nematophila HGB081 are insect pathogenic bacteria and producers of various structurally diverse bioactive natural products. In these entomopathogenic bacteria we investigated the role of the global regulators Lrp, LeuO, and HexA in the production of natural products. Lrp i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00209

    authors: Engel Y,Windhorst C,Lu X,Goodrich-Blair H,Bode HB

    更新日期:2017-02-17 00:00:00

  • Automated quantification of the phagocytosis of Aspergillus fumigatus conidia by a novel image analysis algorithm.

    abstract::Studying the pathobiology of the fungus Aspergillus fumigatus has gained a lot of attention in recent years. This is due to the fact that this fungus is a human pathogen that can cause severe diseases, like invasive pulmonary aspergillosis in immunocompromised patients. Because alveolar macrophages belong to the first...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00549

    authors: Kraibooj K,Schoeler H,Svensson CM,Brakhage AA,Figge MT

    更新日期:2015-06-09 00:00:00

  • Rhizosphere Bacterial Community Response to Continuous Cropping of Tibetan Barley.

    abstract::Long-term continuous cropping influences the nutrient of soil and microbiome of the rhizosphere, resulting in the yield decrease of crops. Tibetan barley is a dominant cereal crop cultivated at high altitudes in Tibet. Its growth and yield are negatively affected by continuous cropping; however, the response of the rh...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.551444

    authors: Yao Y,Yao X,An L,Bai Y,Xie D,Wu K

    更新日期:2020-11-30 00:00:00

  • Corrigendum: Elevated Gut Microbiome-Derived Propionate Levels Are Associated With Reduced Sterile Lung Inflammation and Bacterial Immunity in Mice.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2019.00159.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2019.00518

    authors: Tian X,Hellman J,Horswill AR,Crosby HA,Francis KP,Prakash A

    更新日期:2019-03-26 00:00:00

  • Resistome in Lake Bolonha, Brazilian Amazon: Identification of Genes Related to Resistance to Broad-Spectrum Antibiotics.

    abstract::Resistance to antibiotics is one of the most relevant public health concerns in the world. Aquatic environments play an important role because they are reservoirs for antibiotic resistance genes and antibiotic-resistant strains, contributing to the spread of resistance. The present study investigated the resistome in ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00067

    authors: Alves J,Dias L,Mateus J,Marques J,Graças D,Ramos R,Seldin L,Henriques I,Silva A,Folador A

    更新日期:2020-02-04 00:00:00

  • Effects of Polymorphisms in the SjSP-13 Gene of Schistosoma japonicum on Its Diagnostic Efficacy and Immunogenicity.

    abstract::Schistosomiasis japonica is one of the most prevalent parasitic diseases in China. The scarcity of effective diagnostic tools is a major factor that contributes to the high prevalence of schistosomiasis japonica. SjSP-13 is a promising serological diagnostic biomarker of the disease. However, it is unclear whether pol...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01695

    authors: Xu X,Cui X,Zhu L,Li Z,Zhang Y,Ma L,Pan W

    更新日期:2018-07-25 00:00:00

  • Alkyl Protocatechuate-Loaded Nanostructured Lipid Systems as a Treatment Strategy for Paracoccidioides brasiliensis and Paracoccidioides lutzii In Vitro.

    abstract::Dodecyl protocatechuate (dodecyl) is a derivative of protocatechuic acid (3,4-dihydroxybenzoic acid) that possesses anti-oxidant and antifungal properties. Nanostructured lipid systems (NLS) can potentiate the action of many antifungal agents, reducing the required dose and side effects by improving their activity. Th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01048

    authors: Medina-Alarcón KP,Singulani JL,Voltan AR,Sardi JCO,Petrônio MS,Santos MB,Polaquini CR,Regasini LO,Bolzani VS,da Silva DHS,Chorilli M,Mendes-Giannini MJS,Fusco-Almeida AM

    更新日期:2017-06-12 00:00:00

  • viGEN: An Open Source Pipeline for the Detection and Quantification of Viral RNA in Human Tumors.

    abstract::An estimated 17% of cancers worldwide are associated with infectious causes. The extent and biological significance of viral presence/infection in actual tumor samples is generally unknown but could be measured using human transcriptome (RNA-seq) data from tumor samples. We present an open source bioinformatics pipeli...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01172

    authors: Bhuvaneshwar K,Song L,Madhavan S,Gusev Y

    更新日期:2018-06-05 00:00:00

  • Meta-Analysis of Vaginal Microbiome Data Provides New Insights Into Preterm Birth.

    abstract::Preterm birth (PTB) is defined as the birth of an infant before 37 weeks of gestational age. It is the leading cause of perinatal morbidity and mortality worldwide. In this study, we present a comprehensive meta-analysis of vaginal microbiome in PTB. We integrated raw longitudinal 16S rRNA vaginal microbiome data from...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00476

    authors: Kosti I,Lyalina S,Pollard KS,Butte AJ,Sirota M

    更新日期:2020-04-08 00:00:00

  • Important Role of a Putative Lytic Transglycosylase Cj0843c in β-Lactam Resistance in Campylobacter jejuni.

    abstract::Beta-lactam antibiotics are an important class of antibiotics for treating bacterial infections. Despite prevalent β-lactam resistance in Campylobacter jejuni, the leading bacterial cause of human diarrhea in developed countries, molecular mechanism of β-lactam resistance in C. jejuni is still largely unknown. In this...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01292

    authors: Zeng X,Gillespie B,Lin J

    更新日期:2015-11-17 00:00:00

  • The Capacity of the Fecal Microbiota From Malawian Infants to Ferment Resistant Starch.

    abstract::In Low and Middle-Income Countries (LMIC), weaning is associated with environmentally acquired and inflammation-associated enteric disorders. Dietary intake of high amylose maize starch (HAMS) can promote commensal fermentative bacteria and drive the production of short chain fatty acids (SCFAs). By stabilizing commen...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01459

    authors: Wang Y,Mortimer EK,Katundu KGH,Kalanga N,Leong LEX,Gopalsamy GL,Christophersen CT,Richard AC,Shivasami A,Abell GCJ,Young GP,Rogers GB

    更新日期:2019-06-26 00:00:00

  • Molecular Characterization of Reduced Susceptibility to Biocides in Clinical Isolates of Acinetobacter baumannii.

    abstract::Active efflux is regarded as a common mechanism for antibiotic and biocide resistance. However, the role of many drug efflux pumps in biocide resistance in Acinetobacter baumannii remains unknown. Using biocide-resistant A. baumannii clinical isolates, we investigated the incidence of 11 known/putative antimicrobial r...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01836

    authors: Lin F,Xu Y,Chang Y,Liu C,Jia X,Ling B

    更新日期:2017-09-26 00:00:00

  • The Inhibitory Activities and Antiviral Mechanism of Medicinal Plant Ingredient Quercetin Against Grouper Iridovirus Infection.

    abstract::Singapore grouper iridovirus (SGIV) causes high mortality rates in mariculture, and effective treatments against SGIV infection are urgently required. Illicium verum Hook. f. (I. verum) is a well-known medicinal plant with a variety of biological activities. The natural ingredient quercetin isolated from I. verum coul...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.586331

    authors: Liu M,Yu Q,Xiao H,Li M,Huang Y,Zhang Q,Li P

    更新日期:2020-10-16 00:00:00

  • Micronutrient Requirements and Sharing Capabilities of the Human Gut Microbiome.

    abstract::The human gut microbiome harbors a diverse array of metabolic pathways contributing to its development and homeostasis via a complex web of diet-dependent metabolic interactions within the microbial community and host. Genomics-based reconstruction and predictive modeling of these interactions would provide a framewor...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01316

    authors: Rodionov DA,Arzamasov AA,Khoroshkin MS,Iablokov SN,Leyn SA,Peterson SN,Novichkov PS,Osterman AL

    更新日期:2019-06-12 00:00:00

  • Effect of Bifidobacterium breve in Combination With Different Antibiotics on Clostridium difficile.

    abstract::While combinations of probiotics with antibiotics have exhibited beneficial and adverse effects in the treatment of Clostridium difficile infection (CDI), no substantive explanation has been provided for these effects. In this study, C. difficile ATCC 9689 (CD) was treated with Bifidobacterium breve (YH68) in combinat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02953

    authors: Yang J,Yang H

    更新日期:2018-12-04 00:00:00

  • Evaluation of surrogate animal models of melioidosis.

    abstract::Burkholderia pseudomallei is the Gram-negative bacterial pathogen responsible for the disease melioidosis. B. pseudomallei establishes disease in susceptible individuals through multiple routes of infection, all of which may proceed to a septicemic disease associated with a high mortality rate. B. pseudomallei opportu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2010.00141

    authors: Warawa JM

    更新日期:2010-12-29 00:00:00

  • Chemically-Mediated Interactions Between Macroalgae, Their Fungal Endophytes, and Protistan Pathogens.

    abstract::Filamentous fungi asymptomatically colonize the inner tissues of macroalgae, yet their ecological roles remain largely underexplored. Here, we tested if metabolites produced by fungal endophytes might protect their host against a phylogenetically broad spectrum of protistan pathogens. Accordingly, the cultivable funga...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03161

    authors: Vallet M,Strittmatter M,Murúa P,Lacoste S,Dupont J,Hubas C,Genta-Jouve G,Gachon CMM,Kim GH,Prado S

    更新日期:2018-12-21 00:00:00

  • Thaumarchaeal ammonium oxidation and evidence for a nitrogen cycle in a subsurface radioactive thermal spring in the Austrian Central Alps.

    abstract::Previous studies had suggested the presence of ammonium oxidizing Thaumarchaeota as well as nitrite oxidizing Bacteria in the subsurface spring called Franz Josef Quelle (FJQ), a slightly radioactive thermal mineral spring with a temperature of 43.6-47°C near the alpine village of Bad Gastein, Austria. The microbiolog...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00225

    authors: Gerbl FW,Weidler GW,Wanek W,Erhardt A,Stan-Lotter H

    更新日期:2014-05-16 00:00:00

  • Diversity of D-Amino Acid Utilizing Bacteria From Kongsfjorden, Arctic and the Metabolic Pathways for Seven D-Amino Acids.

    abstract::D-amino acids (DAAs) are an important component of the refractory dissolved organic matter pool in the ocean. Microbes play a vital role in promoting the recycling of DAAs in the ocean. However, the diversity of marine DAA-utilizing bacteria and how they metabolize DAAs are seldom studied. Here, by enrichment culture ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02983

    authors: Yu Y,Yang J,Zheng LY,Sheng Q,Li CY,Wang M,Zhang XY,McMinn A,Zhang YZ,Song XY,Chen XL

    更新日期:2020-01-10 00:00:00

  • Beyond phage display: non-traditional applications of the filamentous bacteriophage as a vaccine carrier, therapeutic biologic, and bioconjugation scaffold.

    abstract::For the past 25 years, phage display technology has been an invaluable tool for studies of protein-protein interactions. However, the inherent biological, biochemical, and biophysical properties of filamentous bacteriophage, as well as the ease of its genetic manipulation, also make it an attractive platform outside t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00755

    authors: Henry KA,Arbabi-Ghahroudi M,Scott JK

    更新日期:2015-08-04 00:00:00

  • Cell Cycle Regulatory Functions of the KSHV Oncoprotein LANA.

    abstract::Manipulation of cell cycle is a commonly employed strategy of viruses for achieving a favorable cellular environment during infection. Kaposi's sarcoma-associated herpesvirus (KSHV), the primary etiological agent of several human malignancies including Kaposi's sarcoma, and primary effusion lymphoma, encodes several o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00334

    authors: Wei F,Gan J,Wang C,Zhu C,Cai Q

    更新日期:2016-03-30 00:00:00

  • Vibrio campbellii hmgA-mediated pyomelanization impairs quorum sensing, virulence, and cellular fitness.

    abstract::Melanization due to the inactivation of the homogentisate-1,2-dioxygenase gene (hmgA) has been demonstrated to increase stress resistance, persistence, and virulence in some bacterial species but such pigmented mutants have not been observed in pathogenic members of the Vibrio Harveyi clade. In this study, we used Vib...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00379

    authors: Wang Z,Lin B,Mostaghim A,Rubin RA,Glaser ER,Mittraparp-Arthorn P,Thompson JR,Vuddhakul V,Vora GJ

    更新日期:2013-12-11 00:00:00

  • Challenging Oil Bioremediation at Deep-Sea Hydrostatic Pressure.

    abstract::The Deepwater Horizon accident has brought oil contamination of deep-sea environments to worldwide attention. The risk for new deep-sea spills is not expected to decrease in the future, as political pressure mounts to access deep-water fossil reserves, and poorly tested technologies are used to access oil. This also a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01203

    authors: Scoma A,Yakimov MM,Boon N

    更新日期:2016-08-03 00:00:00

  • Changes in the Fungal Microbiome of Maize During Hermetic Storage in the United States and Kenya.

    abstract::Prior to harvest, maize kernels are invaded by a diverse population of fungal organisms that comprise the microbiome of the grain mass. Poor post-harvest practices and improper drying can lead to the growth of mycotoxigenic storage fungi and deterioration of grain quality. Hermetic storage bags are a low-cost technolo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02336

    authors: Lane B,Sharma S,Niu C,Maina AW,Wagacha JM,Bluhm BH,Woloshuk CP

    更新日期:2018-10-02 00:00:00

  • Analysis of Difference in Microbial Community and Physicochemical Indices Between Surface and Central Parts of Chinese Special-Flavor Baijiu Daqu.

    abstract::Special-flavor Baijiu is a unique Baijiu in Jiangxi Province, China, whose uniqueness mainly depends on the unique production process of special-flavor Baijiu Daqu. However, the microbial structure and physicochemical indices of different parts of the special-flavor Baijiu Daqu are still unknown. This greatly reduces ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.592421

    authors: Chen Y,Li K,Liu T,Li R,Fu G,Wan Y,Zheng F

    更新日期:2021-01-14 00:00:00

  • A Mosaic of Geothermal and Marine Features Shapes Microbial Community Structure on Deception Island Volcano, Antarctica.

    abstract::Active volcanoes in Antarctica contrast with their predominantly cold surroundings, resulting in environmental conditions capable of selecting for versatile and extremely diverse microbial communities. This is especially true on Deception Island, where geothermal, marine, and polar environments combine to create an ex...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00899

    authors: Bendia AG,Signori CN,Franco DC,Duarte RTD,Bohannan BJM,Pellizari VH

    更新日期:2018-05-07 00:00:00

  • Induction of Salt Tolerance in Arabidopsis thaliana by Volatiles From Bacillus amyloliquefaciens FZB42 via the Jasmonic Acid Signaling Pathway.

    abstract::Previously, we showed that Bacillus amyloliquefaciens FZB42 can confer salt tolerance in plants by root inoculation under salt stress condition, and the FZB42 volatile organic compounds (VOCs) promoted plant growth and development under non-salt stress condition. In the present study, we investigated the mechanism tha...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.562934

    authors: Liu S,Tian Y,Jia M,Lu X,Yue L,Zhao X,Jin W,Wang Y,Zhang Y,Xie Z,Wang R

    更新日期:2020-11-12 00:00:00

  • Long-Term Oil Contamination Alters the Molecular Ecological Networks of Soil Microbial Functional Genes.

    abstract::With knowledge on microbial composition and diversity, investigation of within-community interactions is a further step to elucidate microbial ecological functions, such as the biodegradation of hazardous contaminants. In this work, microbial functional molecular ecological networks were studied in both contaminated a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00060

    authors: Liang Y,Zhao H,Deng Y,Zhou J,Li G,Sun B

    更新日期:2016-02-03 00:00:00

  • Phosphorus Deficiency Inhibits Cell Division But Not Growth in the Dinoflagellate Amphidinium carterae.

    abstract::Phosphorus (P) is an essential nutrient element for the growth of phytoplankton. How P deficiency affects population growth and the cell division cycle in dinoflagellates has only been studied in some species, and how it affects photosynthesis and cell growth remains poorly understood. In the present study, we investi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00826

    authors: Li M,Shi X,Guo C,Lin S

    更新日期:2016-06-01 00:00:00