Effects of Polymorphisms in the SjSP-13 Gene of Schistosoma japonicum on Its Diagnostic Efficacy and Immunogenicity.

Abstract:

:Schistosomiasis japonica is one of the most prevalent parasitic diseases in China. The scarcity of effective diagnostic tools is a major factor that contributes to the high prevalence of schistosomiasis japonica. SjSP-13 is a promising serological diagnostic biomarker of the disease. However, it is unclear whether polymorphisms in SjSP-13 affect its diagnostic efficacy and immunogenicity. Here, we found the SjSP-13 gene was highly polymorphic, and all the alleles of the gene were clustered into two clades, clade A and B. SjSP-13.6 and SjSP-13.25, the representative alleles of clade A and B, were produced in Escherichia coli. The diagnostic value of SjSP-13.6 (AUC = 0.983 ± 0.006), was found to be similar to the SjSP-13.25 (AUC = 0.973 ± 0.009) by receiver operating characteristic (ROC) analysis. SjSP-13.6 and SjSP-13.25 have the same specificity (96.7%), while the sensitivity of SjSP-13.6 (90.4%) is slightly but not significantly higher than SjSP-13.25 (85.2%). The combination use of the two alleles (SjSP-13.6/25) didn't increase the diagnostic performance of SjSP-13 as the AUC value of SjSP-13.6/25 is 0.977 ± 0.009, lower than individual SjSP-13.6 (AUC = 0.983 ± 0.006). In addition, we found the immunogenicity of clade A alleles is significantly higher than clade B in Schistosoma japonicum naturally infected animals and patients, as the mean antibody levels of SjSP-13.6 was significantly higher than SjSP-13.25. We conclude that polymorphisms of the SjSP-13 gene should not affect its diagnostic efficacy, and it is not necessary to combine the alleles of the two clades for diagnosis of schistosomiasis.

journal_name

Front Microbiol

authors

Xu X,Cui X,Zhu L,Li Z,Zhang Y,Ma L,Pan W

doi

10.3389/fmicb.2018.01695

subject

Has Abstract

pub_date

2018-07-25 00:00:00

pages

1695

issn

1664-302X

journal_volume

9

pub_type

杂志文章
  • Immunodeficiency Promotes Adaptive Alterations of Host Gut Microbiome: An Observational Metagenomic Study in Mice.

    abstract::The crosstalk between the gut microbiota and immune state of the host is an essential focus in academia and clinics. To explore the dynamic role of the microbiota in response to immune deficiency, we comprehensively assessed the microbiome of 90 mouse fecal samples, across three time points including two immunodeficie...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02415

    authors: Zheng S,Zhao T,Yuan S,Yang L,Ding J,Cui L,Xu M

    更新日期:2019-11-01 00:00:00

  • Update on Pyrin Functions and Mechanisms of Familial Mediterranean Fever.

    abstract::Mutations in the MEFV gene, which encodes the protein named pyrin (also called marenostrin or TRIM20), are associated with the autoinflammatory disease familial Mediterranean fever (FMF). Recent genetic and immunologic studies uncovered novel functions of pyrin and raised several new questions in relation to FMF patho...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00456

    authors: Manukyan G,Aminov R

    更新日期:2016-03-31 00:00:00

  • Different Transcriptional Responses from Slow and Fast Growth Rate Strains of Listeria monocytogenes Adapted to Low Temperature.

    abstract::Listeria monocytogenes has become one of the principal foodborne pathogens worldwide. The capacity of this bacterium to grow at low temperatures has opened an interesting field of study in terms of the identification and classification of new strains of L. monocytogenes with different growth capacities at low temperat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00229

    authors: Cordero N,Maza F,Navea-Perez H,Aravena A,Marquez-Fontt B,Navarrete P,Figueroa G,González M,Latorre M,Reyes-Jara A

    更新日期:2016-03-01 00:00:00

  • Genomics of Serratia marcescens Isolates Causing Outbreaks in the Same Pediatric Unit 47 Years Apart: Position in an Updated Phylogeny of the Species.

    abstract::The first documented nosocomial outbreak caused by Serratia marcescens in Spain occurred in 1969 at the neonatal intensive care unit (NICU) of the tertiary La Paz Children's Hospital in Madrid, Spain, and based on the available phenotyping techniques at this time, it was considered as a monoclonal outbreak. Only 47 ye...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00451

    authors: Saralegui C,Ponce-Alonso M,Pérez-Viso B,Moles Alegre L,Escribano E,Lázaro-Perona F,Lanza VF,de Pipaón MS,Rodríguez JM,Baquero F,Del Campo R

    更新日期:2020-03-31 00:00:00

  • Construction and Use of Transposon MycoTetOP 2 for Isolation of Conditional Mycobacteria Mutants.

    abstract::Mycobacteria are unique in many aspects of their biology. The development of genetic tools to identify genes critical for their growth by forward genetic analysis holds great promises to advance our understanding of their cellular, physiological and biochemical processes. Here we report the development of a novel tran...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03091

    authors: Riggs-Shute SD,Falkinham JO,Yang Z

    更新日期:2020-01-21 00:00:00

  • Rv2031c of Mycobacterium tuberculosis: a master regulator of Rv2028-Rv2031 (HspX) operon.

    abstract::Genes belonging to the same operon are transcribed as a single mRNA molecule in all prokaryotes. The genes of the same operon are presumed to be involved in similar metabolic and physiological processes. Hence, computational analysis of constituent proteins could provide important clues to the functional relationships...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00351

    authors: Mushtaq K,Sheikh JA,Amir M,Khan N,Singh B,Agrewala JN

    更新日期:2015-04-27 00:00:00

  • Chemical Dispersant Enhances Microbial Exopolymer (EPS) Production and Formation of Marine Oil/Dispersant Snow in Surface Waters of the Subarctic Northeast Atlantic.

    abstract::A notable feature of the Deepwater Horizon oil spill was the unprecedented formation of marine oil snow (MOS) that was observed in large quantities floating on the sea surface and that subsequently sedimented to the seafloor. Whilst the physical and chemical processes involved in MOS formation remain unclear, some stu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00553

    authors: Suja LD,Chen X,Summers S,Paterson DM,Gutierrez T

    更新日期:2019-03-20 00:00:00

  • Experimental Aspects Suggesting a "Fluxus" of Information in the Virions of Herpes Simplex Virus Populations.

    abstract::Our perspective on nature has changed throughout history and at the same time has affected directly or indirectly our perception of biological processes. In that sense, the "fluxus" of information in a viral population arises a result of a much more complex process than the encoding of a protein by a gene, but as the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02625

    authors: Scolaro LA,Roldan JS,Theaux C,Damonte EB,Carlucci MJ

    更新日期:2017-12-22 00:00:00

  • A novel assay for the detection of bioactive volatiles evaluated by screening of lichen-associated bacteria.

    abstract::Volatile organic compounds (VOCs) produced by microorganisms are known both for their effect on pathogens and their role as mediators in various interactions and communications. Previous studies have demonstrated the importance of VOCs for ecosystem functioning as well as their biotechnological potential, but screenin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00398

    authors: Cernava T,Aschenbrenner IA,Grube M,Liebminger S,Berg G

    更新日期:2015-05-01 00:00:00

  • Laser-induced speckle scatter patterns in Bacillus colonies.

    abstract::Label-free bacterial colony phenotyping technology called BARDOT (Bacterial Rapid Detection using Optical scattering Technology) provided successful classification of several different bacteria at the genus, species, and serovar level. Recent experiments with colonies of Bacillus species provided strikingly different ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00537

    authors: Kim H,Singh AK,Bhunia AK,Bae E

    更新日期:2014-10-14 00:00:00

  • Expanding the Current Knowledge About the Role of Interleukin-10 to Major Concerning Bacteria.

    abstract::Interleukin-10 (IL-10) is one of the most important anti-inflammatory cytokine produced during bacterial infection. Two related phenomena explain the importance of IL-10 production in this context: first, the wide range of cells able to produce this cytokine and second, the wide effects that it causes on target cells....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02047

    authors: Peñaloza HF,Noguera LP,Riedel CA,Bueno SM

    更新日期:2018-09-18 00:00:00

  • Role of Bacteriophages in the Implementation of a Sustainable Dairy Chain.

    abstract::The growing human population is currently facing an unprecedented challenge regarding global food sustainability. Thus, it is of paramount to maintain food production and quality while avoiding a negative impact on climate change and the environment at large. Along the food chain, several practices could compromise fu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.00012

    authors: Gutiérrez D,Fernández L,Rodríguez A,García P

    更新日期:2019-01-22 00:00:00

  • Genomic Characterization of Extended-Spectrum Cephalosporin-Resistant Salmonella enterica in the Colombian Poultry Chain.

    abstract::Salmonella enterica serovars have been isolated from Colombian broilers and broiler meat. The aim of this study was to investigate the diversity of ESBL/pAmpC genes in extended-spectrum cephalosporin resistant Salmonella enterica and the phylogeny of ESBL/pAmpC-carrying Salmonella using Whole Genome Sequencing (WGS). ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02431

    authors: Castellanos LR,van der Graaf-van Bloois L,Donado-Godoy P,León M,Clavijo V,Arévalo A,Bernal JF,Mevius DJ,Wagenaar JA,Zomer A,Hordijk J

    更新日期:2018-10-26 00:00:00

  • The Dynamic Distribution of Small-Tail Han Sheep Microbiota across Different Intestinal Segments.

    abstract::The sheep intestinal tract is characterized by a diverse microbial ecosystem that is vital for the host to digest diet material. The importance of gut microbiota (GM) of animals has also been widely acknowledged because of its pivotal roles in the health and well-being of animals. However, there are no relevant studie...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00032

    authors: Zhang H,Shao M,Huang H,Wang S,Ma L,Wang H,Hu L,Wei K,Zhu R

    更新日期:2018-01-31 00:00:00

  • A Homeobox Transcription Factor UvHOX2 Regulates Chlamydospore Formation, Conidiogenesis, and Pathogenicity in Ustilaginoidea virens.

    abstract::Rice false smut fungus (teleomorph: Villosiclava virens; anamorph: Ustilaginoidea virens) can generate chlamydospores and survive winter under field conditions. The chlamydospore is considered as an important infection source of the disease. However, little is known about the regulatory mechanism of the chlamydospore ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01071

    authors: Yu J,Yu M,Song T,Cao H,Pan X,Yong M,Qi Z,Du Y,Zhang R,Yin X,Liu Y

    更新日期:2019-06-20 00:00:00

  • Groundwater Isolation Governs Chemistry and Microbial Community Structure along Hydrologic Flowpaths.

    abstract::This study deals with the effects of hydrodynamic functioning of hard-rock aquifers on microbial communities. In hard-rock aquifers, the heterogeneous hydrologic circulation strongly constrains groundwater residence time, hydrochemistry, and nutrient supply. Here, residence time and a wide range of environmental facto...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01457

    authors: Ben Maamar S,Aquilina L,Quaiser A,Pauwels H,Michon-Coudouel S,Vergnaud-Ayraud V,Labasque T,Roques C,Abbott BW,Dufresne A

    更新日期:2015-12-22 00:00:00

  • Glycan cross-feeding activities between bifidobacteria under in vitro conditions.

    abstract::Bifidobacteria colonize the gut of various mammals, including humans, where they may metabolize complex, diet-, and host-derived carbohydrates. The glycan-associated metabolic features encoded by bifidobacteria are believed to be strongly influenced by cross-feeding activities due to the co-existence of strains with d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01030

    authors: Turroni F,Özcan E,Milani C,Mancabelli L,Viappiani A,van Sinderen D,Sela DA,Ventura M

    更新日期:2015-09-24 00:00:00

  • Rapid Detection of Extended-Spectrum β-Lactamases (ESBL) and AmpC β-Lactamases in Enterobacterales: Development of a Screening Panel Using the MALDI-TOF MS-Based Direct-on-Target Microdroplet Growth Assay.

    abstract::Introduction: Antibiotic resistant bacteria are a growing concern worldwide. Extended-spectrum β-lactamases (ESBL) represent the most common resistance mechanism of Gram-negative bacteria against β-lactams, underlining the need for adequate diagnostic methods that provide reliable information in the shortest time poss...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00013

    authors: Correa-Martínez CL,Idelevich EA,Sparbier K,Kostrzewa M,Becker K

    更新日期:2019-01-24 00:00:00

  • Molecular Networking-Based Metabolome and Bioactivity Analyses of Marine-Adapted Fungi Co-cultivated With Phytopathogens.

    abstract::Fungi represent a rich source of bioactive metabolites and some are marketed as alternatives to synthetic agrochemicals against plant pathogens. However, the culturability of fungal strains in artificial laboratory conditions is still limited and the standard mono-cultures do not reflect their full spectrum chemical d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02072

    authors: Oppong-Danquah E,Parrot D,Blümel M,Labes A,Tasdemir D

    更新日期:2018-09-06 00:00:00

  • Zika Virus Infection at Different Pregnancy Stages: Anatomopathological Findings, Target Cells and Viral Persistence in Placental Tissues.

    abstract::Zika virus (ZIKV) infection in humans has been associated with congenital malformations and other neurological disorders, such as Guillain-Barré syndrome. The mechanism(s) of ZIKV intrauterine transmission, the cell types involved, the most vulnerable period of pregnancy for severe outcomes from infection and other ph...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02266

    authors: de Noronha L,Zanluca C,Burger M,Suzukawa AA,Azevedo M,Rebutini PZ,Novadzki IM,Tanabe LS,Presibella MM,Duarte Dos Santos CN

    更新日期:2018-09-25 00:00:00

  • An In vitro Study of Bio-Control and Plant Growth Promotion Potential of Salicaceae Endophytes.

    abstract::Microbial communities in the endosphere of Salicaceae plants, poplar (Populus trichocarpa) and willow (Salix sitchensis), have been demonstrated to be important for plant growth promotion, protection from biotic and abiotic stresses, and degradation of toxic compounds. Our study aimed to investigate bio-control activi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00386

    authors: Kandel SL,Firrincieli A,Joubert PM,Okubara PA,Leston ND,McGeorge KM,Mugnozza GS,Harfouche A,Kim SH,Doty SL

    更新日期:2017-03-13 00:00:00

  • Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South Korea.

    abstract::This study aimed to describe the distribution and characterization of fecal extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli isolates from healthy companion animals and cohabiting humans. A total of 968 rectal swab samples from 340 participants, including healthy companion animals and cohabiti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00674

    authors: Hong JS,Song W,Park HM,Oh JY,Chae JC,Jeong S,Jeong SH

    更新日期:2020-04-15 00:00:00

  • Diversity and Distribution of Prokaryotes within a Shallow-Water Pockmark Field.

    abstract::Pockmarks are crater-like depression on the seafloor associated with hydrocarbon ascent through muddy sediments in continental shelves around the world. In this study, we examine the diversity and distribution of benthic microbial communities at shallow-water pockmarks adjacent to the Middle Adriatic Ridge. We integra...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00941

    authors: Giovannelli D,d'Errico G,Fiorentino F,Fattorini D,Regoli F,Angeletti L,Bakran-Petricioli T,Vetriani C,Yücel M,Taviani M,Manini E

    更新日期:2016-06-17 00:00:00

  • Antimicrobial Resistance in Wildlife in Guadeloupe (French West Indies): Distribution of a Single bla CTX-M-1/IncI1/ST3 Plasmid Among Humans and Wild Animals.

    abstract::Limited data are available on the contribution of wildlife to the spread of antibacterial resistance. We determined the prevalence of resistance to antibiotics in Escherichia coli isolates collected from wild animals in 2013 and 2014 and the genetic basis for resistance to third-generation cephalosporin in Guadeloupe....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01524

    authors: Guyomard-Rabenirina S,Reynaud Y,Pot M,Albina E,Couvin D,Ducat C,Gruel G,Ferdinand S,Legreneur P,Le Hello S,Malpote E,Sadikalay S,Talarmin A,Breurec S

    更新日期:2020-07-10 00:00:00

  • Comparative Analysis of Virulence and Toxin Expression of Vancomycin-Intermediate and Vancomycin-Sensitive Staphylococcus aureus Strains.

    abstract::Previous studies on vancomycin-intermediate Staphylococcus aureus (VISA) have mainly focused on drug resistance, the evolution of differences in virulence between VISA and vancomycin-sensitive S. aureus (VSSA) requires further investigation. To address this issue, in this study, we compared the virulence and toxin pro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.596942

    authors: Jin Y,Yu X,Zhang S,Kong X,Chen W,Luo Q,Zheng B,Xiao Y

    更新日期:2020-10-30 00:00:00

  • Halotolerant PGPR Stenotrophomonas maltophilia BJ01 Induces Salt Tolerance by Modulating Physiology and Biochemical Activities of Arachis hypogaea.

    abstract::Arachis hypogaea (Peanut) is one of the most important cash crops grown for food and oil production. Salinity is a major constraint for loss of peanut productivity, and halotolerant plant growth promoting bacteria not only enhance plant-growth but also provide tolerance against salt stress. The potential of halotolera...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.568289

    authors: Alexander A,Singh VK,Mishra A

    更新日期:2020-10-14 00:00:00

  • The Diverse Functional Roles of Elongation Factor Tu (EF-Tu) in Microbial Pathogenesis.

    abstract::Elongation factor thermal unstable Tu (EF-Tu) is a G protein that catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome inside living cells. Structural and biochemical studies have described the complex interactions needed to effect canonical function. However, EF-Tu has evolved the capacity to execute...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.02351

    authors: Harvey KL,Jarocki VM,Charles IG,Djordjevic SP

    更新日期:2019-10-24 00:00:00

  • Isolation and Molecular Characterization of Novel Chlorpyrifos and 3,5,6-trichloro-2-pyridinol-degrading Bacteria from Sugarcane Farm Soils.

    abstract::Chlorpyrifos (CP) is one of the most widely used organophosphate pesticides in agriculture worldwide, but its extensive use has led to the contamination of various soil and water systems. Microbial bioremediation is considered to be one of the most viable options for the removal of CP from the environment; however, li...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00518

    authors: Rayu S,Nielsen UN,Nazaries L,Singh BK

    更新日期:2017-04-04 00:00:00

  • Characterization of Chemosynthetic Microbial Mats Associated with Intertidal Hydrothermal Sulfur Vents in White Point, San Pedro, CA, USA.

    abstract::The shallow-sea hydrothermal vents at White Point (WP) in Palos Verdes on the southern California coast support microbial mats and provide easily accessed settings in which to study chemolithoautotrophic sulfur cycling. Previous studies have cultured sulfur-oxidizing bacteria from the WP mats; however, almost nothing ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01163

    authors: Miranda PJ,McLain NK,Hatzenpichler R,Orphan VJ,Dillon JG

    更新日期:2016-07-27 00:00:00

  • Functional Biology and Molecular Mechanisms of Host-Pathogen Interactions for Aflatoxin Contamination in Groundnut (Arachis hypogaea L.) and Maize (Zea mays L.).

    abstract::Aflatoxins are secondary metabolites produced by soilborne saprophytic fungus Aspergillus flavus and closely related species that infect several agricultural commodities including groundnut and maize. The consumption of contaminated commodities adversely affects the health of humans and livestock. Aflatoxin contaminat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.00227

    authors: Soni P,Gangurde SS,Ortega-Beltran A,Kumar R,Parmar S,Sudini HK,Lei Y,Ni X,Huai D,Fountain JC,Njoroge S,Mahuku G,Radhakrishnan T,Zhuang W,Guo B,Liao B,Singam P,Pandey MK,Bandyopadhyay R,Varshney RK

    更新日期:2020-03-03 00:00:00