当前位置： SCI文献检索 > Science Signaling期刊下所有文献 > Pressure-induced oxidative activation of PKG enables vasoregulation by Ca2+ sparks and BK channels.

Pressure-induced oxidative activation of PKG enables vasoregulation by Ca2+ sparks and BK channels.

Abstract：

:Activation of Ca2+-sensitive, large-conductance potassium (BK) channels in vascular smooth muscle cells (VSMCs) by local, ryanodine receptor-mediated Ca2+ signals (Ca2+ sparks) acts as a brake on pressure-induced (myogenic) vasoconstriction-a fundamental mechanism that regulates blood flow in small resistance arteries. We report that physiological intraluminal pressure within resistance arteries activated cGMP-dependent protein kinase (PKG) in VSMCs through oxidant-induced formation of an intermolecular disulfide bond between cysteine residues. Oxidant-activated PKG was required to trigger Ca2+ sparks, BK channel activity, and vasodilation in response to pressure. VSMCs from arteries from mice expressing a form of PKG that could not be activated by oxidants showed reduced Ca2+ spark frequency, and arterial preparations from these mice had decreased pressure-induced activation of BK channels. Thus, the absence of oxidative activation of PKG disabled the BK channel-mediated negative feedback regulation of vasoconstriction. Our results support the concept of a negative feedback control mechanism that regulates arterial diameter through mechanosensitive production of oxidants to activate PKG and enhance Ca2+ sparks.

journal_name

Sci Signal

journal_title

Science signaling

authors

Khavandi K,Baylie RA,Sugden SA,Ahmed M,Csato V,Eaton P,Hill-Eubanks DC,Bonev AD,Nelson MT,Greenstein AS

doi

10.1126/scisignal.aaf6625

subject

Has Abstract

pub_date

2016-10-11 00:00:00

pages

ra100

issue

449

eissn

1945-0877

issn

1937-9145

journal_volume

9

pub_type

杂志文章

相关文献

Science Signaling文献大全
  • Redundant and specialized roles for diacylglycerol kinases α and ζ in the control of T cell functions.

    abstract：:The diacylglycerol kinases (DGKs) attenuate diacylglycerol (DAG)-mediated signals by catalyzing the conversion of DAG to phosphatidic acid. In T lymphocytes, the antigen-stimulated generation of DAG links signal strength to the intensity and duration of signaling by the Ras-extracellular signal-regulated kinase (ERK) ...

    journal_title：Science signaling

    pub_type： 杂志文章,评审

    doi：10.1126/scisignal.aaa0974

    authors： Mérida I,Andrada E,Gharbi SI,Ávila-Flores A

    更新日期：2015-04-28 00:00:00

  • Hypusine biosynthesis in β cells links polyamine metabolism to facultative cellular proliferation to maintain glucose homeostasis.

    abstract：:Deoxyhypusine synthase (DHPS) uses the polyamine spermidine to catalyze the hypusine modification of the mRNA translation factor eIF5A and promotes oncogenesis through poorly defined mechanisms. Because germline deletion of Dhps is embryonically lethal, its role in normal postnatal cellular function in vivo remains un...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aax0715

    authors： Levasseur EM,Yamada K,Piñeros AR,Wu W,Syed F,Orr KS,Anderson-Baucum E,Mastracci TL,Maier B,Mosley AL,Liu Y,Bernal-Mizrachi E,Alonso LC,Scott D,Garcia-Ocaña A,Tersey SA,Mirmira RG

    更新日期：2019-12-03 00:00:00

  • The early synthesis of p35 and activation of CDK5 in LPS-stimulated macrophages suppresses interleukin-10 production.

    abstract：:Interleukin-10 (IL-10) is an important anti-inflammatory cytokine that is produced primarily by macrophages. We investigated mechanisms by which the timing of IL-10 production was controlled in macrophages and found that cyclin-dependent kinase 5 (CDK5) activity was markedly increased in lipopolysaccharide (LPS)-stimu...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aab3156

    authors： Na YR,Jung D,Gu GJ,Jang AR,Suh YH,Seok SH

    更新日期：2015-11-24 00:00:00

  • The pseudokinase MLKL activates PAD4-dependent NET formation in necroptotic neutrophils.

    abstract：:Neutrophil extracellular trap (NET) formation can generate short-term, functional anucleate cytoplasts and trigger loss of cell viability. We demonstrated that the necroptotic cell death effector mixed lineage kinase domain-like (MLKL) translocated from the cytoplasm to the plasma membrane and stimulated downstream NA...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aao1716

    authors： D'Cruz AA,Speir M,Bliss-Moreau M,Dietrich S,Wang S,Chen AA,Gavillet M,Al-Obeidi A,Lawlor KE,Vince JE,Kelliher MA,Hakem R,Pasparakis M,Williams DA,Ericsson M,Croker BA

    更新日期：2018-09-04 00:00:00

  • Rounding up the usual suspects in O2 sensing: CO, NO, and H2S!

    abstract：:Oxygen sensing by the carotid body is essential in vertebrates to adapt to reduced arterial oxygen tension. In this issue of Science Signaling, Yuan et al. report an intricate signaling system to transduce a physical parameter--oxygen tension--into a biological cellular signal (neural discharge) through changes in the...

    journal_title：Science signaling

    pub_type： 评论,杂志文章

    doi：10.1126/scisignal.aab1665

    authors： Fandrey J

    更新日期：2015-04-21 00:00:00

  • Long-term potentiation modulates synaptic phosphorylation networks and reshapes the structure of the postsynaptic interactome.

    abstract：:The postsynaptic site of neurons is composed of more than 1500 proteins arranged in protein-protein interaction complexes, the composition of which is modulated by protein phosphorylation through the actions of complex signaling networks. Components of these networks function as key regulators of synaptic plasticity, ...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aaf6716

    authors： Li J,Wilkinson B,Clementel VA,Hou J,O'Dell TJ,Coba MP

    更新日期：2016-08-09 00:00:00

  • Tumor suppression by PTEN requires the activation of the PKR-eIF2alpha phosphorylation pathway.

    abstract：:Inhibition of protein synthesis by phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2) at Ser(51) occurs as a result of the activation of a family of kinases in response to various forms of stress. Although some consequences of eIF2alpha phosphorylation are cytoprotective, phospho...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.2000389

    authors： Mounir Z,Krishnamoorthy JL,Robertson GP,Scheuner D,Kaufman RJ,Georgescu MM,Koromilas AE

    更新日期：2009-12-22 00:00:00

  • An "inordinate fondness for transporters" explained?

    abstract：:An often-asked question is, Why are there so many different transporters in a cell to take up a particular substrate? At least part of the answer comes from work on the possible competitive advantage of dual-transporter systems. In such systems, low-affinity transporters function when a nutrient is plentiful in the en...

    journal_title：Science signaling

    pub_type： 杂志文章,评审

    doi：10.1126/scisignal.2002837

    authors： Eide DJ

    更新日期：2012-02-07 00:00:00

  • FZD5 is a Gαq-coupled receptor that exhibits the functional hallmarks of prototypical GPCRs.

    abstract：:Frizzleds (FZDs) are a group of seven transmembrane-spanning (7TM) receptors that belong to class F of the G protein-coupled receptor (GPCR) superfamily. FZDs bind WNT proteins to stimulate diverse signaling cascades involved in embryonic development, stem cell regulation, and adult tissue homeostasis. Frizzled 5 (FZD...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aar5536

    authors： Wright SC,Cañizal MCA,Benkel T,Simon K,Le Gouill C,Matricon P,Namkung Y,Lukasheva V,König GM,Laporte SA,Carlsson J,Kostenis E,Bouvier M,Schulte G,Hoffmann C

    更新日期：2018-12-04 00:00:00

  • Quantitative analysis of competitive cytokine signaling predicts tissue thresholds for the propagation of macrophage activation.

    abstract：:Toll-like receptor (TLR) signaling regulates macrophage activation and effector cytokine propagation in the constrained environment of a tissue. In macrophage populations, TLR4 stimulates the dose-dependent transcription of nuclear factor κB (NF-κB) target genes. However, using single-RNA counting, we found that indiv...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aaf3998

    authors： Bagnall J,Boddington C,England H,Brignall R,Downton P,Alsoufi Z,Boyd J,Rowe W,Bennett A,Walker C,Adamson A,Patel NMX,O'Cualain R,Schmidt L,Spiller DG,Jackson DA,Müller W,Muldoon M,White MRH,Paszek P

    更新日期：2018-07-24 00:00:00

  • Tumors block pain with CXCL12.

    abstract：:The chemokine CXCL12 released from early stage pancreatic cancer recruits Schwann cells and suppresses pain signaling. ...

    journal_title：Science signaling

    pub_type： 评论,杂志文章

    doi：10.1126/scisignal.aam7233

    authors： Gough NR

    更新日期：2017-01-10 00:00:00

  • TGF-β-mediated enhancement of TH17 cell generation is inhibited by bone morphogenetic protein receptor 1α signaling.

    abstract：:The cytokines of the transforming growth factor-β (TGF-β) family promote the growth and differentiation of multiple tissues, but the role of only the founding member, TGF-β, in regulating the immune responses has been extensively studied. TGF-β is critical to prevent the spontaneous activation of self-reactive T cells...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aar2125

    authors： Browning LM,Pietrzak M,Kuczma M,Simms CP,Kurczewska A,Refugia JM,Lowery DJ,Rempala G,Gutkin D,Ignatowicz L,Muranski P,Kraj P

    更新日期：2018-08-28 00:00:00

  • Inositol pyrophosphates as mammalian cell signals.

    abstract：:Inositol pyrophosphates are highly energetic inositol polyphosphate molecules present in organisms from slime molds and yeast to mammals. Distinct classes of enzymes generate different forms of inositol pyrophosphates. The biosynthesis of these substances principally involves phosphorylation of inositol hexakisphospha...

    journal_title：Science signaling

    pub_type： 杂志文章,评审

    doi：10.1126/scisignal.2001958

    authors： Chakraborty A,Kim S,Snyder SH

    更新日期：2011-08-23 00:00:00

  • Brx mediates the response of lymphocytes to osmotic stress through the activation of NFAT5.

    abstract：:Extracellular hyperosmolarity, or osmotic stress, generally caused by differences in salt and macromolecule concentrations across the plasma membrane, occurs in lymphoid organs and at inflammatory sites. The response of immune cells to osmotic stress is regulated by nuclear factor of activated T cells 5 (NFAT5), a tra...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.2000081

    authors： Kino T,Takatori H,Manoli I,Wang Y,Tiulpakov A,Blackman MR,Su YA,Chrousos GP,DeCherney AH,Segars JH

    更新日期：2009-02-10 00:00:00

  • Chronic activation of mTOR complex 1 is sufficient to cause hepatocellular carcinoma in mice.

    abstract：:The mammalian target of rapamycin (mTOR) complex 1 (mTORC1) is a nutrient-sensitive protein kinase that is aberrantly activated in many human cancers. Whether dysregulation of mTORC1 signaling in normal tissues increases the risk for cancer, however, is unknown. We focused on hepatocellular carcinoma, which has been l...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.2002739

    authors： Menon S,Yecies JL,Zhang HH,Howell JJ,Nicholatos J,Harputlugil E,Bronson RT,Kwiatkowski DJ,Manning BD

    更新日期：2012-03-27 00:00:00

  • Hug and hold tight: Dimerization controls the turnover of the ubiquitin-conjugating enzyme UBE2S.

    abstract：:Precise control of the activity and abundance of ubiquitin-conjugating enzymes (E2s) ensures fidelity in ubiquitin chain synthesis. In this issue of Science Signaling, Liess et al. demonstrate that the human anaphase-promoting complex (APC/C)-associated E2 UBE2S adopts an autoinhibited dimeric state that increases the...

    journal_title：Science signaling

    pub_type： 杂志文章,评审

    doi：10.1126/scisignal.abd9892

    authors： Bremm A

    更新日期：2020-10-20 00:00:00

  • Drosophila toll pathway: the new model.

    abstract：:In Drosophila, recognition of microbe-specific molecules (such as bacterial peptidoglycans) activates serine protease cascades that converge to activate the Toll pathway. Recent data show that the serine protease Grass, which is activated downstream of pattern recognition receptors and was initially thought to be a co...

    journal_title：Science signaling

    pub_type： 杂志文章,评审

    doi：10.1126/scisignal.252jc1

    authors： Ashok Y

    更新日期：2009-01-06 00:00:00

  • A large bioactive BMP ligand with distinct signaling properties is produced by alternative proconvertase processing.

    abstract：:Dimers of conventional transforming growth factor-β (TGF-β) and bone morphogenetic protein (BMP) ligands are composed of two 100- to 140-amino acid peptides that are produced through the proteolytic processing of a proprotein precursor by proconvertases, such as furin. We report the identification of an evolutionarily...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.2002549

    authors： Akiyama T,Marqués G,Wharton KA

    更新日期：2012-04-03 00:00:00

  • Structural basis for activation and inhibition of class I phosphoinositide 3-kinases.

    abstract：:Phosphoinositide 3-kinases (PI3Ks) are implicated in a broad spectrum of cellular activities, such as growth, proliferation, differentiation, migration, and metabolism. Activation of class I PI3Ks by mutation or overexpression correlates with the development and maintenance of various human cancers. These PI3Ks are he...

    journal_title：Science signaling

    pub_type： 杂志文章,评审

    doi：10.1126/scisignal.2002165

    authors： Vadas O,Burke JE,Zhang X,Berndt A,Williams RL

    更新日期：2011-10-18 00:00:00

  • Loss of MCU prevents mitochondrial fusion in G1-S phase and blocks cell cycle progression and proliferation.

    abstract：:The role of the mitochondrial Ca2+ uniporter (MCU) in physiologic cell proliferation remains to be defined. Here, we demonstrated that the MCU was required to match mitochondrial function to metabolic demands during the cell cycle. During the G1-S transition (the cycle phase with the highest mitochondrial ATP output),...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aav1439

    authors： Koval OM,Nguyen EK,Santhana V,Fidler TP,Sebag SC,Rasmussen TP,Mittauer DJ,Strack S,Goswami PC,Abel ED,Grumbach IM

    更新日期：2019-04-30 00:00:00

  • The role of EHD proteins at the neuronal synapse.

    abstract：:Eps15 homology domain (EHD) proteins are conserved adenosine triphosphatases that are involved in membrane remodeling. EHD family members are structurally similar to the guanosine triphosphatase (GTPase) dynamin, and both are essential for the fission step of clathrin-mediated endocytosis. This Journal Club highlights...

    journal_title：Science signaling

    pub_type： 杂志文章,评审

    doi：10.1126/scisignal.2002989

    authors： Ioannou MS,Marat AL

    更新日期：2012-04-24 00:00:00

  • Regulation of type I interferon: It's HIP to be K2.

    abstract：:Uncontrolled expression of type I interferon (IFN-I) drives autoimmunity, necessitating the need for tight regulation. In this issue, Cao et al reveal a role for the kinase HIPK2 in the transcriptional control of IFN-I during antiviral immune responses. ...

    journal_title：Science signaling

    pub_type： 评论,杂志文章,评审

    doi：10.1126/scisignal.aaw8549

    authors： Best SM,Ponia SS

    更新日期：2019-03-19 00:00:00

  • Itk controls the spatiotemporal organization of T cell activation.

    abstract：:During T cell activation by antigen-presenting cells (APCs), the diverse spatiotemporal organization of components of T cell signaling pathways modulates the efficiency of activation. Here, we found that loss of the tyrosine kinase interleukin-2 (IL-2)-inducible T cell kinase (Itk) in mice altered the spatiotemporal d...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.2001821

    authors： Singleton KL,Gosh M,Dandekar RD,Au-Yeung BB,Ksionda O,Tybulewicz VL,Altman A,Fowell DJ,Wülfing C

    更新日期：2011-10-04 00:00:00

  • The HIF-1 alpha-C/EBP alpha axis.

    abstract：:The hypoxia inducible factors (HIFs) and CCAAT/enhancer binding protein alpha (C/EBP alpha) are transcription factors that mediate adaptive responses to hypoxia and control aspects of energy metabolism, respectively. New evidence suggests that when HIF-1 alpha and C/EBP alpha interact, they bring about reciprocal func...

    journal_title：Science signaling

    pub_type： 杂志文章,评审

    doi：10.1126/scisignal.143jc2

    authors： Janardhan HP

    更新日期：2008-10-28 00:00:00

  • AML suppresses hematopoiesis by releasing exosomes that contain microRNAs targeting c-MYB.

    abstract：:Exosomes are paracrine regulators of the tumor microenvironment and contain complex cargo. We previously reported that exosomes released from acute myeloid leukemia (AML) cells can suppress residual hematopoietic stem and progenitor cell (HSPC) function indirectly through stromal reprogramming of niche retention facto...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aaf2797

    authors： Hornick NI,Doron B,Abdelhamed S,Huan J,Harrington CA,Shen R,Cambronne XA,Chakkaramakkil Verghese S,Kurre P

    更新日期：2016-09-06 00:00:00

  • Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localization.

    abstract：:The deubiquitylating enzyme OTUB1 is present in all tissues and targets many substrates, in both the cytosol and nucleus. We found that casein kinase 2 (CK2) phosphorylated OTUB1 at Ser(16) to promote its nuclear accumulation in cells. Pharmacological inhibition or genetic ablation of CK2 blocked the phosphorylation o...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aaa0441

    authors： Herhaus L,Perez-Oliva AB,Cozza G,Gourlay R,Weidlich S,Campbell DG,Pinna LA,Sapkota GP

    更新日期：2015-04-14 00:00:00

  • A direct screen for c-di-GMP modulators reveals a Salmonella Typhimurium periplasmic ʟ-arginine-sensing pathway.

    abstract：:Cyclic-di-GMP (c-di-GMP) is a bacterial second messenger that transduces internal and external signals and regulates bacterial motility and biofilm formation. Some organisms encode more than 100 c-di-GMP-modulating enzymes, but only for a few has a signal been defined that modulates their activity. We developed and ap...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aaa1796

    authors： Mills E,Petersen E,Kulasekara BR,Miller SI

    更新日期：2015-06-09 00:00:00

  • Dynamic palmitoylation controls the microdomain localization of the DKK1 receptors CKAP4 and LRP6.

    abstract：:Dickkopf1 (DKK1) was originally identified as an antagonist of Wnt signaling that binds to and induces the clathrin-mediated endocytosis of the Wnt coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP5/6). DKK1 also binds to cytoskeleton-associated protein 4 (CKAP4), which was originally identifi...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aat9519

    authors： Sada R,Kimura H,Fukata Y,Fukata M,Yamamoto H,Kikuchi A

    更新日期：2019-11-19 00:00:00

  • FOXP1 potentiates Wnt/β-catenin signaling in diffuse large B cell lymphoma.

    abstract：:The transcription factor FOXP1 (forkhead box protein P1) is a master regulator of stem and progenitor cell biology. In diffuse large B cell lymphoma (DLBCL), copy number amplifications and chromosomal translocations result in overexpression of FOXP1. Increased abundance of FOXP1 in DLBCL is a predictor of poor prognos...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.2005654

    authors： Walker MP,Stopford CM,Cederlund M,Fang F,Jahn C,Rabinowitz AD,Goldfarb D,Graham DM,Yan F,Deal AM,Fedoriw Y,Richards KL,Davis IJ,Weidinger G,Damania B,Major MB

    更新日期：2015-02-03 00:00:00

  • A nanoscale reorganization of the IL-15 receptor is triggered by NKG2D in a ligand-dependent manner.

    abstract：:Natural killer group 2D (NKG2D), an activating receptor on natural killer (NK) cells and a subset of T cells, recognizes stress-inducible proteins, including MICA and ULBP2, which are present on infected or transformed cells. Whether each NKG2D ligand (NKG2DL) has a distinct biological role is not clear. Using superre...

    journal_title：Science signaling

    pub_type： 杂志文章

    doi：10.1126/scisignal.aal3606

    authors： Bálint Š,Lopes FB,Davis DM

    更新日期：2018-04-10 00:00:00